ATE157703T1 - Endoplasmatisches retikulum-ständige rekombinante dibasische endoprotease und deren verwendungen - Google Patents

Endoplasmatisches retikulum-ständige rekombinante dibasische endoprotease und deren verwendungen

Info

Publication number
ATE157703T1
ATE157703T1 AT92810964T AT92810964T ATE157703T1 AT E157703 T1 ATE157703 T1 AT E157703T1 AT 92810964 T AT92810964 T AT 92810964T AT 92810964 T AT92810964 T AT 92810964T AT E157703 T1 ATE157703 T1 AT E157703T1
Authority
AT
Austria
Prior art keywords
recombinant
permanent
dibasic
endoprotease
endoplasmatic reticulum
Prior art date
Application number
AT92810964T
Other languages
English (en)
Inventor
Bhabatosh Dr Chaudhuri
Christine Stephan
Peter Dr Seeboth
Howard Prof Dr Riezman
Original Assignee
Ciba Geigy Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ciba Geigy Ag filed Critical Ciba Geigy Ag
Application granted granted Critical
Publication of ATE157703T1 publication Critical patent/ATE157703T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/04—Fusion polypeptide containing a localisation/targetting motif containing an ER retention signal such as a C-terminal HDEL motif
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Endocrinology (AREA)
  • Diabetes (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
AT92810964T 1991-12-16 1992-12-08 Endoplasmatisches retikulum-ständige rekombinante dibasische endoprotease und deren verwendungen ATE157703T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP91810984 1991-12-16

Publications (1)

Publication Number Publication Date
ATE157703T1 true ATE157703T1 (de) 1997-09-15

Family

ID=8208912

Family Applications (1)

Application Number Title Priority Date Filing Date
AT92810964T ATE157703T1 (de) 1991-12-16 1992-12-08 Endoplasmatisches retikulum-ständige rekombinante dibasische endoprotease und deren verwendungen

Country Status (17)

Country Link
US (2) US5501975A (de)
EP (1) EP0548012B1 (de)
JP (1) JP3305781B2 (de)
KR (1) KR100270283B1 (de)
AT (1) ATE157703T1 (de)
AU (1) AU667852B2 (de)
CA (1) CA2085308C (de)
DE (1) DE69222013T2 (de)
DK (1) DK0548012T3 (de)
ES (1) ES2107520T3 (de)
FI (1) FI105482B (de)
GR (1) GR3025324T3 (de)
IL (1) IL104075A (de)
MX (1) MX9207291A (de)
NO (1) NO308619B1 (de)
NZ (1) NZ245469A (de)
ZA (1) ZA929711B (de)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9404270D0 (en) * 1994-03-05 1994-04-20 Delta Biotechnology Ltd Yeast strains and modified albumins
EP0812362A1 (de) * 1995-02-27 1997-12-17 Abbott Laboratories Plasmid zur expression von modifizierten rekombinanten proteinen in einem bakteriellen system
US5942254A (en) * 1995-02-27 1999-08-24 Abbott Laboratories Phosphorylated recombinant human β-casein expressed in a bacterial system
EP0812361A1 (de) * 1995-02-27 1997-12-17 Abbott Laboratories Verfahren zur expression von modifizierten rekombinanten proteinen in einem bakteriellen system
US5807702A (en) * 1995-02-27 1998-09-15 Abbott Laboratories Method for expressing phosphorylated recombinant human β-casein in a bacterial system
US5710044A (en) * 1995-02-27 1998-01-20 Abbott Laboratories Plasmid for expressing phosphorylated recombinant proteins in a bacterial system
AT404838B (de) * 1995-11-24 1999-03-25 Immuno Ag Herstellung von proteinen aus pro-proteinen durch fusionsproteine abgeleitet von furin oder furinanalogen
FR2751000B1 (fr) 1996-07-12 1998-10-30 Inst Nat Sante Rech Med Adn specifiques des bacteries de l'espece neisseria meningitidis, leurs procedes d'obtention et leurs applications biologiques
JP4463988B2 (ja) * 1998-11-06 2010-05-19 ノボ ノルディスク ヘルス ケア アクチェンゲゼルシャフト Ｖｉｉ因子の生産方法
EP2028275A3 (de) 2000-06-30 2009-05-06 VIB vzw Modifikation der Proteinglycosylierung in Pichia Pastoris
DE10221411B4 (de) * 2002-05-14 2004-07-08 GSF-Forschungszentrum für Umwelt und Gesundheit GmbH Rekombinantes Fowlpox-Virus
US20040117874A1 (en) * 2002-10-15 2004-06-17 Jianjun Yang Methods for accumulating translocated proteins
US7507573B2 (en) 2003-11-14 2009-03-24 Vib, Vzw Modification of protein glycosylation in methylotrophic yeast
AU2005323451B2 (en) * 2004-05-21 2010-11-18 Qiagen Sciences Llc Kits and processes for removing contaminants from nucleic acids in environmental and biological samples
US20080082338A1 (en) * 2006-09-29 2008-04-03 O'neil Michael P Systems and methods for secure voice identification and medical device interface
RU2344173C2 (ru) * 2006-10-27 2009-01-20 Институт биохимии им. А.Н. Баха Российской Академии Наук Кассета и рекомбинантная плазмида для зкспрессии и секреции механозависимого фактора роста человека (mgf), штамм saccharomyces cerevisiae - продуцент mgf и способ получения mgf
WO2014138371A1 (en) 2013-03-06 2014-09-12 Glaxosmithkline Llc Host cells and methods of use

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE78262T1 (de) * 1985-06-11 1992-08-15 Ciba Geigy Ag Hybrid-interferone.
US5391485A (en) * 1985-08-06 1995-02-21 Immunex Corporation DNAs encoding analog GM-CSF molecules displaying resistance to proteases which cleave at adjacent dibasic residues
FI100106B (fi) * 1986-12-05 1997-09-30 Novartis Ag Menetelmä plasminogeenin yksisäikeisen yhdistelmäaktivaattorin valmist amiseksi
JP2643968B2 (ja) * 1988-02-03 1997-08-25 サントリー株式会社 Ｋｅｘ２エンドプロテアーゼ及びその製造方法

Also Published As

Publication number Publication date
JP3305781B2 (ja) 2002-07-24
FI925678L (fi) 1993-06-17
IL104075A (en) 2003-01-12
ES2107520T3 (es) 1997-12-01
GR3025324T3 (en) 1998-02-27
NO924847L (no) 1993-06-17
JPH06197787A (ja) 1994-07-19
NO924847D0 (no) 1992-12-15
DK0548012T3 (da) 1998-04-14
ZA929711B (en) 1993-06-16
AU667852B2 (en) 1996-04-18
AU3007192A (en) 1993-06-17
US5618690A (en) 1997-04-08
US5501975A (en) 1996-03-26
KR100270283B1 (ko) 2000-11-01
EP0548012B1 (de) 1997-09-03
FI925678A0 (fi) 1992-12-14
DE69222013D1 (de) 1997-10-09
MX9207291A (es) 1993-07-01
DE69222013T2 (de) 1998-01-29
KR930013115A (ko) 1993-07-21
IL104075A0 (en) 1993-05-13
EP0548012A1 (de) 1993-06-23
CA2085308A1 (en) 1993-06-17
CA2085308C (en) 2003-12-02
NZ245469A (en) 1995-03-28
NO308619B1 (no) 2000-10-02
FI105482B (fi) 2000-08-31

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UEP Publication of translation of european patent specification