ATE159762T1 - Verfahren zur dns-sequenzbestimmung mit dns- polymerase von thermus aquaticus - Google Patents

Verfahren zur dns-sequenzbestimmung mit dns- polymerase von thermus aquaticus

Info

Publication number
ATE159762T1
ATE159762T1 AT89910787T AT89910787T ATE159762T1 AT E159762 T1 ATE159762 T1 AT E159762T1 AT 89910787 T AT89910787 T AT 89910787T AT 89910787 T AT89910787 T AT 89910787T AT E159762 T1 ATE159762 T1 AT E159762T1
Authority
AT
Austria
Prior art keywords
thermus aquaticus
dna
sequence determination
dna polymerase
dna sequence
Prior art date
Application number
AT89910787T
Other languages
English (en)
Inventor
Michael A Innis
Kenneth B Myambo
David H Gelfand
Mary Ann D Brow
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22943169&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE159762(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of ATE159762T1 publication Critical patent/ATE159762T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/807—Apparatus included in process claim, e.g. physical support structures
    • Y10S436/808—Automated or kit

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT89910787T 1988-09-23 1989-09-19 Verfahren zur dns-sequenzbestimmung mit dns- polymerase von thermus aquaticus ATE159762T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/249,367 US5075216A (en) 1988-09-23 1988-09-23 Methods for dna sequencing with thermus aquaticus dna polymerase

Publications (1)

Publication Number Publication Date
ATE159762T1 true ATE159762T1 (de) 1997-11-15

Family

ID=22943169

Family Applications (1)

Application Number Title Priority Date Filing Date
AT89910787T ATE159762T1 (de) 1988-09-23 1989-09-19 Verfahren zur dns-sequenzbestimmung mit dns- polymerase von thermus aquaticus

Country Status (8)

Country Link
US (1) US5075216A (de)
EP (1) EP0437459B1 (de)
JP (1) JP2901194B2 (de)
AT (1) ATE159762T1 (de)
AU (1) AU647015B2 (de)
CA (1) CA1332561C (de)
DE (1) DE68928413T2 (de)
WO (1) WO1990003442A1 (de)

Families Citing this family (148)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5333675C1 (en) * 1986-02-25 2001-05-01 Perkin Elmer Corp Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
US5656493A (en) * 1985-03-28 1997-08-12 The Perkin-Elmer Corporation System for automated performance of the polymerase chain reaction
US5173411A (en) * 1987-01-14 1992-12-22 President And Fellows Of Harvard College Method for determining the nucleotide base sequence of a DNA molecule
US5547839A (en) * 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
KR100236506B1 (ko) * 1990-11-29 2000-01-15 퍼킨-엘머시터스인스트루먼츠 폴리머라제 연쇄 반응 수행 장치
DE4214112A1 (de) * 1991-08-02 1993-02-04 Europ Lab Molekularbiolog Neues verfahren zur sequenzierung von nukleinsaeuren
US5674679A (en) * 1991-09-27 1997-10-07 Amersham Life Science, Inc. DNA cycle sequencing
US5830642A (en) * 1992-04-03 1998-11-03 Amersham Life Science, Inc. Electrophoresis of nucleic acid fragments
US5314595A (en) * 1992-04-03 1994-05-24 United States Biochemical Corporation Electrophoresis of nucleic acid fragments
US6410277B1 (en) 1993-02-19 2002-06-25 Takara Shuzo Co., Ltd. DNA polymersases with enhanced length of primer extension
US7465539B1 (en) 1993-02-19 2008-12-16 Takara Bio, Inc. DNA polymerases with enhanced length of primer extension
US5436149A (en) * 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US5550040A (en) * 1993-06-23 1996-08-27 Hoffman-La Roche Inc. Method, reagents and kits for the detection of Neisseria gonorrhoeae
US5601990A (en) * 1994-09-13 1997-02-11 Thomas Jefferson University Methods of diagnosing colorectal tumors and metastasis thereof
WO1995011694A1 (en) * 1993-10-26 1995-05-04 Thomas Jefferson University Compositions that specifically bind to colorectal cancer cells and methods of using the same
US7097839B1 (en) * 1993-10-26 2006-08-29 Thomas Jefferson University ST receptor binding compounds and methods of using the same
US5879656A (en) * 1993-10-26 1999-03-09 Thomas Jefferson University Methods of treating metastatic colorectal cancer with ST receptor binding compounds
US7312052B1 (en) * 1993-12-08 2007-12-25 Stratagene California Polymerase compositions and uses thereof
US5480783A (en) * 1994-03-31 1996-01-02 The Perkin-Elmer Corporation Method for reducing background signals in DNA replication/detection assays
US6455251B1 (en) 1994-09-13 2002-09-24 Thomas Jefferson University Methods of and kits and compositions for diagnosing colorectal tumors and metastasis thereof
US5919617A (en) * 1994-12-21 1999-07-06 Miami University Methods and reagents for detecting fungal pathogens in a biological sample
CA2222744C (en) * 1995-05-31 2008-03-25 Amersham Life Science, Inc. Thermostable dna polymerases
US5876949A (en) * 1995-05-31 1999-03-02 The Trustees Of The University Of Pennsylvania Antibodies specific for fragile X related proteins and method of using the same
US6077664A (en) * 1995-06-07 2000-06-20 Promega Corporation Thermophilic DNA polymerases from Thermotoga neapolitana
US6001645A (en) * 1995-06-07 1999-12-14 Promega Corporation Thermophilic DNA polymerases from thermotoga neapolitana
US5750345A (en) * 1995-10-31 1998-05-12 Evanston Hospital Corporation Detection of human α-thalassemia mutations and their use as predictors of blood-related disorders
US5830657A (en) * 1996-05-01 1998-11-03 Visible Genetics Inc. Method for single-tube sequencing of nucleic acid polymers
US5888736A (en) * 1995-12-22 1999-03-30 Visible Genetics, Inc. Method, compositions and kit for detection and identification of microorganisms
US6168918B1 (en) 1996-01-31 2001-01-02 American Home Products Corp. Method of detecting foreign DNA integrated in eukaryotic chromosomes
US6413718B1 (en) 1996-05-01 2002-07-02 Visible Genetics Inc. Method for sequencing of nucleic acid polymers
US6602659B1 (en) 1996-05-03 2003-08-05 Thomas Jefferson University Methods of and kits and compositions for diagnosing colorectal tumors and metastasis thereof
US6455248B1 (en) 1996-05-20 2002-09-24 Miami University Reagents and kits for detecting fungal pathogens in a biological sample
US5910409A (en) * 1996-05-20 1999-06-08 Miami University Methods and reagents for detecting fungal pathogens in a biological sample
US5849546A (en) * 1996-09-13 1998-12-15 Epicentre Technologies Corporation Methods for using mutant RNA polymerases with reduced discrimination between non-canonical and canonical nucleoside triphosphates
US6855545B1 (en) 1996-10-04 2005-02-15 Lexicon Genetics Inc. Indexed library of cells containing genomic modifications and methods of making and utilizing the same
US6139833A (en) * 1997-08-08 2000-10-31 Lexicon Genetics Incorporated Targeted gene discovery
US6207371B1 (en) 1996-10-04 2001-03-27 Lexicon Genetics Incorporated Indexed library of cells containing genomic modifications and methods of making and utilizing the same
US7332338B2 (en) 1996-10-04 2008-02-19 Lexicon Pharmaceuticals, Inc. Vectors for making genomic modifications
US20040072243A1 (en) * 1996-10-11 2004-04-15 Lexicon Genetics Incorporated Indexed library of cells containing genomic modifications and methods of making and utilizing the same
US6291164B1 (en) 1996-11-22 2001-09-18 Invitrogen Corporation Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate
DE19653439A1 (de) * 1996-12-20 1998-07-02 Svante Dr Paeaebo Verfahren zur direkten, exponentiellen Amplifikation und Sequenzierung von DNA Molekülen und dessen Anwendung
US20030215857A1 (en) * 1996-12-20 2003-11-20 Roche Diagnostics Gmbh Method for the direct, exponential amplification and sequencing of DNA molecules and its application
US6605428B2 (en) 1996-12-20 2003-08-12 Roche Diagnostics Gmbh Method for the direct, exponential amplification and sequencing of DNA molecules and its application
DE19653494A1 (de) * 1996-12-20 1998-06-25 Svante Dr Paeaebo Verfahren zur entkoppelten, direkten, exponentiellen Amplifikation und Sequenzierung von DNA Molekülen unter Zugabe einer zweiten thermostabilen DNA Polymerase und dessen Anwendung
US5952200A (en) * 1997-02-06 1999-09-14 University Of South Carolina Method of diagnosing cancer in human cells using a reverse transcriptase-polymerase chain reaction for identifying the presence of stromelysin-3
WO1998035707A1 (en) 1997-02-18 1998-08-20 Thomas Jefferson University Compositions that bind to pancreatic cancer cells and methods of using the same
EP1007718B1 (de) 1997-03-21 2007-11-14 Stratagene California Polymerase-verbessernder faktor (pef)-enthaltende extrakte, pef proteinkomplexe, isoliertes pef protein und verfahren zur reinigung und identifizierung
US6235471B1 (en) 1997-04-04 2001-05-22 Caliper Technologies Corp. Closed-loop biochemical analyzers
US6391622B1 (en) 1997-04-04 2002-05-21 Caliper Technologies Corp. Closed-loop biochemical analyzers
CA2284612A1 (en) * 1997-04-04 1998-10-15 Michael Knapp Closed-loop biochemical analyzers
US7135333B1 (en) 1997-08-07 2006-11-14 Thomas Jefferson University Compositions that specifically bind to colorectal cancer cells and methods of using the same
US6120995A (en) * 1997-08-07 2000-09-19 Thomas Jefferson University Compositions that specifically bind to colorectal cancer cells and methods of using the same
US6103468A (en) * 1997-10-07 2000-08-15 Labatt Brewing Company Limited Rapid two-stage polymerase chain reaction method for detection of lactic acid bacteria in beer
WO1999040226A2 (en) * 1998-02-04 1999-08-12 Perkin-Elmer Corporation Determination of a genotype of an amplification product at multiple allelic sites
US6436707B1 (en) * 1998-03-27 2002-08-20 Lexicon Genetics Incorporated Vectors for gene mutagenesis and gene discovery
US6808921B1 (en) * 1998-03-27 2004-10-26 Lexicon Genetics Incorporated Vectors for gene mutagenesis and gene discovery
WO1999058724A1 (en) * 1998-05-08 1999-11-18 Life Technologies, Inc. A method for synthesizing a nucleic acid molecule using a ribonuclease
US7056713B1 (en) 1998-08-06 2006-06-06 Duke University Urate oxidase
US6333403B1 (en) * 1998-11-06 2001-12-25 Myriad Genetics, Inc. Chromosome 17p-linked prostate cancer susceptibility gene and a paralog and orthologous genes
EP1074633A1 (de) * 1999-07-05 2001-02-07 LION Bioscience AG Nukleotide und ihre Verwendung in der Sequenzierung von GC-reichen Templaten
GB9922971D0 (en) * 1999-09-29 1999-12-01 Secr Defence Reaction system
US6893819B1 (en) * 2000-11-21 2005-05-17 Stratagene California Methods for detection of a nucleic acid by sequential amplification
US7118860B2 (en) 1999-10-29 2006-10-10 Stratagene California Methods for detection of a target nucleic acid by capture
US7824859B2 (en) * 1999-10-29 2010-11-02 Cytyc Corporation Methods for detection of a target nucleic acid by forming a cleavage structure using an RNA polymerase
US7838225B2 (en) 1999-10-29 2010-11-23 Hologic, Inc. Methods for detection of a target nucleic acid by forming a cleavage structure using a reverse transcriptase
WO2001061015A2 (de) 2000-02-17 2001-08-23 Qiagen Gmbh Thermostable chimeric nucleic acid polymerases and uses thereof
US6436677B1 (en) 2000-03-02 2002-08-20 Promega Corporation Method of reverse transcription
US6632645B1 (en) 2000-03-02 2003-10-14 Promega Corporation Thermophilic DNA polymerases from Thermoactinomyces vulgaris
EP1274861B1 (de) * 2000-03-27 2009-12-23 Thomas Jefferson University Zusammensetzungen und methoden zur identifizierung von krebszellen
JP4721606B2 (ja) * 2000-03-30 2011-07-13 トヨタ自動車株式会社 単一核酸分子の塩基配列決定法
US20080242627A1 (en) * 2000-08-02 2008-10-02 University Of Southern California Novel rna interference methods using dna-rna duplex constructs
US7662791B2 (en) 2000-08-02 2010-02-16 University Of Southern California Gene silencing using mRNA-cDNA hybrids
WO2002022885A1 (en) * 2000-09-18 2002-03-21 Thomas Jefferson University Compositions and methods for identifying and targeting stomach and esophageal cancer cells
DE10049211A1 (de) * 2000-10-05 2002-04-18 Qiagen Gmbh Thermostabile Polymerase aus Thermococcus pacificus
GB0110476D0 (en) * 2001-04-30 2001-06-20 Secr Defence Reagent delivery system
US6905827B2 (en) 2001-06-08 2005-06-14 Expression Diagnostics, Inc. Methods and compositions for diagnosing or monitoring auto immune and chronic inflammatory diseases
US7235358B2 (en) 2001-06-08 2007-06-26 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US7026121B1 (en) 2001-06-08 2006-04-11 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US20030129709A1 (en) * 2001-11-02 2003-07-10 Olga Makarova Method for site-directed mutagenesis of nucleic acid molecules using a single primer
US7222059B2 (en) * 2001-11-15 2007-05-22 Siemens Medical Solutions Diagnostics Electrophoretic trace simulator
US20030180741A1 (en) 2001-12-21 2003-09-25 Holly Hogrefe High fidelity DNA polymerase compositions and uses therefor
BR0308161A (pt) * 2002-03-01 2006-06-06 Ravgen Inc métodos para a detecção de distúrbios genéticos
US6977162B2 (en) 2002-03-01 2005-12-20 Ravgen, Inc. Rapid analysis of variations in a genome
CN1656234B (zh) * 2002-03-20 2012-02-01 创新生物公司 包囊化核酸扩增反应混合物的微胶囊及其作为反应隔间进行平行反应的用途
US20030228611A1 (en) * 2002-05-01 2003-12-11 President And Fellows Of Harvard College Nucleic acid memory device
US7442506B2 (en) 2002-05-08 2008-10-28 Ravgen, Inc. Methods for detection of genetic disorders
US7727720B2 (en) 2002-05-08 2010-06-01 Ravgen, Inc. Methods for detection of genetic disorders
US20030210489A1 (en) * 2002-05-13 2003-11-13 Yec Co., Ltd. Data eraser and data erasing program
AU2004225520A1 (en) 2003-03-25 2004-10-14 Stratagene DNA polymerase fusions and uses thereof
US7820030B2 (en) 2003-04-16 2010-10-26 Handylab, Inc. System and method for electrochemical detection of biological compounds
DE602004028924D1 (de) 2003-04-21 2010-10-14 Roche Diagnostics Gmbh Polymorphismen des frzb gens und deren zusammenhang mit osteoporose
US7892745B2 (en) 2003-04-24 2011-02-22 Xdx, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
EP1641938A2 (de) * 2003-06-30 2006-04-05 AstraZeneca AB Nukleinsäurepolymerase-fluoreszenztests
US7179602B2 (en) * 2003-09-04 2007-02-20 Los Alamos National Security, Llc Methods for sequencing GC-rich and CCT repeat DNA templates
CN1860232A (zh) * 2003-09-30 2006-11-08 莱克康遗传公司 鉴别基因功能的方法和组合物
US20050244847A1 (en) * 2003-11-26 2005-11-03 Eppendorf Ag Methods and compositions for in vitro amplification of extrachromosomal nucleic acid
US8293471B2 (en) * 2004-01-28 2012-10-23 Marshall University Research Corporation Apparatus and method for a continuous rapid thermal cycle system
US7645575B2 (en) 2004-09-08 2010-01-12 Xdx, Inc. Genes useful for diagnosing and monitoring inflammation related disorders
EP1812604A4 (de) * 2004-11-03 2011-02-23 Third Wave Tech Inc Ein-schritt-nachweistest
US7051536B1 (en) * 2004-11-12 2006-05-30 Bio-Rad Laboratories, Inc. Thermal cycler with protection from atmospheric moisture
JP2006230342A (ja) * 2005-02-28 2006-09-07 Tokyo Univ Of Agriculture & Technology 一本鎖核酸の分離方法および装置並びにマイクロアレイおよびｄｎａチップ。
GB0514935D0 (en) * 2005-07-20 2005-08-24 Solexa Ltd Methods for sequencing a polynucleotide template
US7939645B2 (en) 2006-01-06 2011-05-10 Agilent Technologies, Inc Reaction buffer composition for nucleic acid replication with packed DNA polymerases
AU2007211847B2 (en) 2006-02-02 2011-01-27 Qiagen Instruments Ag Thermocycler and sample port
US10741034B2 (en) 2006-05-19 2020-08-11 Apdn (B.V.I.) Inc. Security system and method of marking an inventory item and/or person in the vicinity
WO2008021431A2 (en) 2006-08-14 2008-02-21 Xdx, Inc. Methods and compositions for diagnosing and monitoring the status of transplant rejection and immune disorders
EP2102367A2 (de) 2006-11-09 2009-09-23 XDX, Inc. Verfahren zur diagnose und überwachung des status von systemischem lupus erythematodes
US9938641B2 (en) * 2006-12-18 2018-04-10 Fluidigm Corporation Selection of aptamers based on geometry
AU2008222590B2 (en) * 2007-03-02 2013-01-17 Qiagen Instruments Ag Apparatus and method for nucleic acid amplification
US9352320B2 (en) * 2007-08-28 2016-05-31 Qiagen Instruments Ag Thermal cycling device with selectively openable sample port
CN101855365A (zh) * 2007-11-30 2010-10-06 科贝特研究私人有限公司 热循环装置
CA2715170A1 (en) * 2008-02-11 2009-08-20 Hadasit Medical Research Services & Development Limited Colon cancer associated transcript 1 (ccat-1) as a cancer marker
BRPI0908748A2 (pt) * 2008-03-15 2015-07-28 Hologic Inc Composições e métodos para análise de moléculas de ácido nucleico durante reações de amplificação
KR20110036638A (ko) 2008-07-25 2011-04-07 리차드 더블유. 와그너 단백질 스크리닝 방법
CA2638458A1 (en) * 2008-07-31 2010-01-31 Spartan Bioscience Inc. Thermal recycling by positioning relative to fixed-temperature source
US8524490B2 (en) * 2008-09-18 2013-09-03 X-Bar Diagnostic Systems, Inc. Fully automated portable DNA detection system
WO2010062776A2 (en) 2008-11-03 2010-06-03 Kapabiosystems Chimeric dna polymerases
CN102245761A (zh) 2008-11-03 2011-11-16 卡帕生物系统 修饰a型dna聚合酶
US20110070590A1 (en) 2009-09-22 2011-03-24 Jan Rohozinski Primers and Methods for Determining RhD Zygosity
JP2013518590A (ja) 2010-02-02 2013-05-23 アボツト・バイオテクノロジー・リミテツド ＴＮＦ−α阻害剤を用いる処置に対する応答性を予測するための方法および組成物
WO2011127136A1 (en) * 2010-04-06 2011-10-13 University Of Chicago Composition and methods related to modification of 5-hydroxymethylcytosine (5-hmc)
EP2560759B1 (de) 2010-04-20 2020-02-12 QIAGEN GmbH Temperatursteuerungsverfahren und -vorrichtung
US8618253B2 (en) 2010-05-25 2013-12-31 Samsung Techwin Co., Ltd. Modified RNAse H and detection of nucleic acid amplification
WO2012097318A2 (en) 2011-01-14 2012-07-19 Kap Abiosystems Modified dna polymerases for improved amplification
EP2686441B1 (de) 2011-03-18 2019-05-08 Eisai R&D Management Co., Ltd. Verfahren und verwendungen zur vorhersage der reaktion auf eribulin
IN2014CN03647A (de) 2011-10-20 2015-10-09 Novartis Ag
WO2013057308A2 (en) 2011-10-20 2013-04-25 Qiagen Instruments Ag Method for verifying a temperature measurement in a micro-environment and system for verifying a temperature measurement in a micro-environment
EP2827991B1 (de) 2012-03-23 2019-05-29 QIAGEN GmbH Thermocycler und verfahren zur erwärmung und kühlung
US20140024541A1 (en) * 2012-07-17 2014-01-23 Counsyl, Inc. Methods and compositions for high-throughput sequencing
EP2687294A1 (de) 2012-07-18 2014-01-22 Qiagen Instruments AG Rotor für einen Wärmeumwälzer und Wärmeumwälzer
BR112015013771B1 (pt) 2012-12-11 2022-10-04 Novartis Ag Composição que compreende um ativador do receptor alfa 7, nicotínico de acetilcolina (nachr) como biomarcador preditivo de resposta ao tratamento de ativador do dito receptor
US9963740B2 (en) 2013-03-07 2018-05-08 APDN (B.V.I.), Inc. Method and device for marking articles
EP3058339B1 (de) 2013-10-07 2019-05-22 APDN (B.V.I.) Inc. Multimodales bild und spektraler leser
NZ719726A (en) 2013-10-15 2021-07-30 Bio Molecular Systems Pty Ltd Improved thermocycler
US20170073754A1 (en) 2014-02-07 2017-03-16 Novartis Ag Impact of genetic factors on disease progression and response to anti-c5 antibody in geographic atrophy
CN106103121B (zh) 2014-03-18 2019-12-06 亚普蒂恩(B.V.I.)公司 用于安全应用的加密光学标记物
US10745825B2 (en) 2014-03-18 2020-08-18 Apdn (B.V.I.) Inc. Encrypted optical markers for security applications
CN108138243A (zh) 2015-09-18 2018-06-08 西门子医疗保健诊断公司 用于hiv分析的试剂和方法
CN108350407B (zh) 2015-12-11 2022-07-08 杰诺玛迪克斯公司 用于核酸扩增的管密封系统和方法
WO2017180302A1 (en) 2016-04-11 2017-10-19 Apdn (B.V.I.) Inc. Method of marking cellulosic products
US20200182884A1 (en) 2016-06-27 2020-06-11 Juno Therapeutics, Inc. Method of identifying peptide epitopes, molecules that bind such epitopes and related uses
EP3478702A4 (de) 2016-06-27 2020-03-18 Dana-Farber Cancer Institute, Inc. Verfahren zur messung der rna-translationsraten
JP7054397B2 (ja) 2016-06-27 2022-04-13 ジュノー セラピューティクス インコーポレイテッド Ｍｈｃ－ｅ拘束性エピトープ、結合分子ならびに関連する方法および使用
US10995371B2 (en) 2016-10-13 2021-05-04 Apdn (B.V.I.) Inc. Composition and method of DNA marking elastomeric material
US10920274B2 (en) 2017-02-21 2021-02-16 Apdn (B.V.I.) Inc. Nucleic acid coated submicron particles for authentication
US11851679B2 (en) 2017-11-01 2023-12-26 Juno Therapeutics, Inc. Method of assessing activity of recombinant antigen receptors
EP4363611A1 (de) 2021-06-30 2024-05-08 Dana-Farber Cancer Institute, Inc. Zusammensetzungen und verfahren zur anreicherung von nukleinsäuren mit lichtvermittelter vernetzung
WO2025049432A1 (en) 2023-08-28 2025-03-06 Intellia Therapeutics, Inc. Methods for editing hla-a in cells pre-screened for the absence of one or both alleles of hla-h*01

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
CA1339653C (en) * 1986-02-25 1998-02-03 Larry J. Johnson Appartus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
CA1338457C (en) * 1986-08-22 1996-07-16 Henry A. Erlich Purified thermostable enzyme
US4795699A (en) * 1987-01-14 1989-01-03 President And Fellows Of Harvard College T7 DNA polymerase
US4921794A (en) * 1987-01-14 1990-05-01 President And Fellows Of Harvard College T7 DNA polymerase
US4962020A (en) * 1988-07-12 1990-10-09 President And Fellows Of Harvard College DNA sequencing
WO1990008839A1 (en) * 1989-02-06 1990-08-09 Eastman Kodak Company Thermostable polymerase dna sequencing reaction concentrate
US5001050A (en) * 1989-03-24 1991-03-19 Consejo Superior Investigaciones Cientificas PHφ29 DNA polymerase

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EP0437459B1 (de) 1997-10-29
WO1990003442A1 (en) 1990-04-05
JPH04501205A (ja) 1992-03-05
DE68928413T2 (de) 1998-06-04
AU647015B2 (en) 1994-03-17
AU4300389A (en) 1990-04-18
CA1332561C (en) 1994-10-18
US5075216A (en) 1991-12-24
DE68928413D1 (de) 1997-12-04
JP2901194B2 (ja) 1999-06-07
EP0437459A1 (de) 1991-07-24

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