ATE159947T1 - Verfahren und trennsubstrat zur analyse von mononukleotiden und oligonukleotiden - Google Patents

Verfahren und trennsubstrat zur analyse von mononukleotiden und oligonukleotiden

Info

Publication number: ATE159947T1
Authority: AT; Austria
Prior art keywords: oligonucleotides; mononucleotides; analyzing; volume; substrate
Prior art date: 1994-01-07

Application number

AT95906176T

Other languages

English (en)

Inventor

Aharon S Cohen

Andre Bourque

Maria Vilenchik

Original Assignee

Hybridon Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1994-01-07

Filing date

1995-01-04

Publication date

1997-11-15

1995-01-04 Application filed by Hybridon Inc filed Critical Hybridon Inc

1997-11-15 Application granted granted Critical

1997-11-15 Publication of ATE159947T1 publication Critical patent/ATE159947T1/de

Links

239000000758 substrate Substances 0.000 title abstract 4
108091034117 Oligonucleotide Proteins 0.000 title abstract 3
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 title abstract 3
238000000034 method Methods 0.000 title abstract 2
238000000926 separation method Methods 0.000 title 1
239000003960 organic solvent Substances 0.000 abstract 2
XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 abstract 1
239000004202 carbamide Substances 0.000 abstract 1
239000007788 liquid Substances 0.000 abstract 1
229920000642 polymer Polymers 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44747—Composition of gel or of carrier mixture

Landscapes

Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Molecular Biology (AREA)
Engineering & Computer Science (AREA)
Genetics & Genomics (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Biotechnology (AREA)
Biomedical Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
Analytical Chemistry (AREA)
Zoology (AREA)
Physics & Mathematics (AREA)
General Engineering & Computer Science (AREA)
Wood Science & Technology (AREA)
Biophysics (AREA)
Microbiology (AREA)
Plant Pathology (AREA)
Crystallography & Structural Chemistry (AREA)
Dispersion Chemistry (AREA)
Chemical Kinetics & Catalysis (AREA)
Electrochemistry (AREA)
General Physics & Mathematics (AREA)
Immunology (AREA)
Pathology (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Saccharide Compounds (AREA)
Investigating Or Analysing Biological Materials (AREA)

AT95906176T 1994-01-07 1995-01-04 Verfahren und trennsubstrat zur analyse von mononukleotiden und oligonukleotiden ATE159947T1 (de)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US08/178,660 US5627277A (en)	1992-12-16	1994-01-07	Method for analyzing oligonucleotide analogs

Publications (1)

Publication Number	Publication Date
ATE159947T1 true ATE159947T1 (de)	1997-11-15

Family

ID=22653406

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT95906176T ATE159947T1 (de)	1994-01-07	1995-01-04	Verfahren und trennsubstrat zur analyse von mononukleotiden und oligonukleotiden

Country Status (8)

Country	Link
US (1)	US5627277A (de)
EP (1)	EP0738272B1 (de)
JP (1)	JPH09507574A (de)
AT (1)	ATE159947T1 (de)
AU (1)	AU1449395A (de)
CA (1)	CA2177958A1 (de)
DE (1)	DE69500988D1 (de)
WO (1)	WO1995018813A1 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JP2000502734A (ja) *	1995-12-28	2000-03-07	ノバルティスアクチエンゲゼルシャフト	分離剤として充填可能ポリマー溶液を使用するキャピラリー電気泳動分離法
AUPO403496A0 (en) *	1996-12-05	1997-01-02	Macquarie Research Limited	Electrophoresis separation methods
ES2255732T3 (es) *	1997-08-19	2006-07-01	Idera Pharmaceuticals, Inc.	Oligonucleotidos especificos de vih y procedimientos para su utilizacion.
US6465692B1 (en) *	1999-12-22	2002-10-15	Beckman Coulter, Inc.	Reagents for preparing a sample containing biomolecular analytes for capillary electrophoretic separation and methods for making the reagents
US20040222095A1 (en) *	2001-01-12	2004-11-11	Karger Barry L.	Dna separation using linear polymer solutions with dimethyl sulfoxide
EP1533612B1 (de)	2004-07-30	2007-04-11	Agilent Technologies, Inc.	Elektroforetische Gelmatrix enthaltend DMSO
US20260049958A1 (en) *	2022-10-25	2026-02-19	Synthon B.V.	Capillary gel electrophoresis separation of diastereomers of phosphorothioated oligonucleotides

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4865707A (en) *	1986-10-21	1989-09-12	Northeastern University	Capillary gel electrophoresis columns
US4865706A (en) *	1986-10-21	1989-09-12	Northeastern University	High performance microcapillary gel electrophoresis
US5112460A (en) *	1986-10-21	1992-05-12	Northeastern University	High performance microcapillary gel electrophoresis
US4816571A (en) *	1987-06-04	1989-03-28	Applied Biosystems, Inc.	Chemical capping by phosphitylation during oligonucleotide synthesis
US4965349A (en) *	1987-12-24	1990-10-23	Applied Biosystems, Inc.	Method of synthesizing oligonucleotides labeled with ammonia-labile groups on solid phase supports
US5262530A (en) *	1988-12-21	1993-11-16	Applied Biosystems, Inc.	Automated system for polynucleotide synthesis and purification
US5047524A (en) *	1988-12-21	1991-09-10	Applied Biosystems, Inc.	Automated system for polynucleotide synthesis and purification
EP0497448A1 (de) *	1991-02-01	1992-08-05	Beckman Instruments, Inc.	Kapillar-Gel-Elektrophorese in Anwesenheit von mindestens einem von Harnstoff verschiedenen Denaturierungsreagens
US5420265A (en) *	1992-12-16	1995-05-30	Hybridon, Inc.	Separation of phosphorothioate oligonucleotides by capillary gel electrophoresis
US5338428A (en) *	1993-05-28	1994-08-16	California Institute Of Technology	Poly(N-Acylalkylenimine) electrophoresis support media

1994
- 1994-01-07 US US08/178,660 patent/US5627277A/en not_active Expired - Fee Related
1995
- 1995-01-04 DE DE69500988T patent/DE69500988D1/de not_active Expired - Lifetime
- 1995-01-04 WO PCT/US1995/000163 patent/WO1995018813A1/en not_active Ceased
- 1995-01-04 AT AT95906176T patent/ATE159947T1/de not_active IP Right Cessation
- 1995-01-04 EP EP95906176A patent/EP0738272B1/de not_active Expired - Lifetime
- 1995-01-04 CA CA002177958A patent/CA2177958A1/en not_active Abandoned
- 1995-01-04 AU AU14493/95A patent/AU1449395A/en not_active Abandoned
- 1995-01-04 JP JP7518609A patent/JPH09507574A/ja active Pending

Also Published As

Publication number	Publication date
US5627277A (en)	1997-05-06
AU1449395A (en)	1995-08-01
EP0738272B1 (de)	1997-11-05
EP0738272A1 (de)	1996-10-23
JPH09507574A (ja)	1997-07-29
DE69500988D1 (de)	1997-12-11
WO1995018813A1 (en)	1995-07-13
CA2177958A1 (en)	1995-07-13

Legal Events

Date	Code	Title	Description
1997-12-15	REZ	Deleted due to withdrawal
1998-05-15	RER	Ceased as to paragraph 5 lit. 3 law introducing patent treaties

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