ATE161042T1 - Serumfreies medium für die kultur von postnatalen zentralen neuronen - Google Patents

Serumfreies medium für die kultur von postnatalen zentralen neuronen

Info

Publication number: ATE161042T1
Authority: AT; Austria
Prior art keywords: medium; central neurons; serum; culture; free medium
Prior art date: 1994-02-18

Application number

AT95101935T

Other languages

English (en)

Inventor

Hiroshi Saito

Hiroshi Katsuki

Fumio Kawahara

Original Assignee

Kyorin Seiyaku Kk

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1994-02-18

Filing date

1995-02-13

Publication date

1997-12-15

1995-02-13 Application filed by Kyorin Seiyaku Kk filed Critical Kyorin Seiyaku Kk

1997-12-15 Application granted granted Critical

1997-12-15 Publication of ATE161042T1 publication Critical patent/ATE161042T1/de

Links

210000002569 neuron Anatomy 0.000 title abstract 2
239000012679 serum free medium Substances 0.000 title 1
239000002609 medium Substances 0.000 abstract 3
RJKFOVLPORLFTN-LEKSSAKUSA-N Progesterone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H](C(=O)C)[C@@]1(C)CC2 RJKFOVLPORLFTN-LEKSSAKUSA-N 0.000 abstract 2
NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 abstract 2
KIDHWZJUCRJVML-UHFFFAOYSA-N putrescine Chemical compound NCCCCN KIDHWZJUCRJVML-UHFFFAOYSA-N 0.000 abstract 2
101000693922 Bos taurus Albumin Proteins 0.000 abstract 1
239000006145 Eagle's minimal essential medium Substances 0.000 abstract 1
102000004877 Insulin Human genes 0.000 abstract 1
108090001061 Insulin Proteins 0.000 abstract 1
102000003777 Interleukin-1 beta Human genes 0.000 abstract 1
108090000193 Interleukin-1 beta Proteins 0.000 abstract 1
239000005700 Putrescine Substances 0.000 abstract 1
102000004338 Transferrin Human genes 0.000 abstract 1
108090000901 Transferrin Proteins 0.000 abstract 1
102100035140 Vitronectin Human genes 0.000 abstract 1
108010031318 Vitronectin Proteins 0.000 abstract 1
239000003636 conditioned culture medium Substances 0.000 abstract 1
229940125396 insulin Drugs 0.000 abstract 1
238000012423 maintenance Methods 0.000 abstract 1
238000000034 method Methods 0.000 abstract 1
229960003387 progesterone Drugs 0.000 abstract 1
239000000186 progesterone Substances 0.000 abstract 1
239000000758 substrate Substances 0.000 abstract 1
230000004083 survival effect Effects 0.000 abstract 1
239000012581 transferrin Substances 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0618—Cells of the nervous system
- C12N5/0619—Neurons
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/135—Platelet-derived growth factor [PDGF]
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/23—Interleukins [IL]
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/50—Cell markers; Cell surface determinants
- C12N2501/58—Adhesion molecules, e.g. ICAM, VCAM, CD18 (ligand), CD11 (ligand), CD49 (ligand)

Landscapes

Engineering & Computer Science (AREA)
Health & Medical Sciences (AREA)
Biomedical Technology (AREA)
Life Sciences & Earth Sciences (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Biotechnology (AREA)
Organic Chemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
Genetics & Genomics (AREA)
Chemical & Material Sciences (AREA)
Neurology (AREA)
Neurosurgery (AREA)
Microbiology (AREA)
Biochemistry (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Cell Biology (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)

AT95101935T 1994-02-18 1995-02-13 Serumfreies medium für die kultur von postnatalen zentralen neuronen ATE161042T1 (de)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
JP6044955A JPH07227278A (ja)	1994-02-18	1994-02-18	成熟中枢神経細胞用の無血清培地

Publications (1)

Publication Number	Publication Date
ATE161042T1 true ATE161042T1 (de)	1997-12-15

Family

ID=12705916

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT95101935T ATE161042T1 (de)	1994-02-18	1995-02-13	Serumfreies medium für die kultur von postnatalen zentralen neuronen

Country Status (7)

Country	Link
US (1)	US5780301A (de)
EP (1)	EP0668348B1 (de)
JP (1)	JPH07227278A (de)
AT (1)	ATE161042T1 (de)
DE (1)	DE69501165T2 (de)
DK (1)	DK0668348T3 (de)
ES (1)	ES2111346T3 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JP3093974B2 (ja) *	1995-06-27	2000-10-03	住友ベークライト株式会社	神経細胞用培養液、その製造方法及びこれを用いる神経細胞の培養方法
JP3072051B2 (ja) *	1996-06-10	2000-07-31	住友ベークライト株式会社	神経細胞用培養液、その製造方法及びこれを用いる神経細胞の培養方法
US6506576B2 (en)	2001-03-14	2003-01-14	Board Of Trustees Of The University Of Arkansas	Serum-and steroid-free culture media for cerebellar granule neurons
GB0501365D0 (en)	2005-01-21	2005-03-02	Promar As	Compositions

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5135866A (en) *	1989-03-03	1992-08-04	W. R. Grace & Co.-Conn.	Very low protein nutrient medium for cell culture
JPH05252941A (ja) *	1991-08-12	1993-10-05	Sakai Enetsukusu Kk	動物細胞培養用担体
US5405772A (en) *	1993-06-18	1995-04-11	Amgen Inc.	Medium for long-term proliferation and development of cells

1994
- 1994-02-18 JP JP6044955A patent/JPH07227278A/ja active Pending
1995
- 1995-02-13 DE DE69501165T patent/DE69501165T2/de not_active Expired - Fee Related
- 1995-02-13 ES ES95101935T patent/ES2111346T3/es not_active Expired - Lifetime
- 1995-02-13 AT AT95101935T patent/ATE161042T1/de not_active IP Right Cessation
- 1995-02-13 EP EP95101935A patent/EP0668348B1/de not_active Expired - Lifetime
- 1995-02-13 DK DK95101935T patent/DK0668348T3/da active
- 1995-02-13 US US08/387,321 patent/US5780301A/en not_active Expired - Fee Related

Also Published As

Publication number	Publication date
ES2111346T3 (es)	1998-03-01
DE69501165T2 (de)	1998-07-02
JPH07227278A (ja)	1995-08-29
DE69501165D1 (de)	1998-01-22
US5780301A (en)	1998-07-14
DK0668348T3 (da)	1998-08-10
EP0668348A1 (de)	1995-08-23
EP0668348B1 (de)	1997-12-10

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Legal Events

Date	Code	Title	Description
1998-06-15	UEP	Publication of translation of european patent specification
2007-11-15	REN	Ceased due to non-payment of the annual fee