ATE161541T1 - Chemische-modifizierte oligonukleotide für site- spezifisches mutagenesis - Google Patents

Chemische-modifizierte oligonukleotide für site- spezifisches mutagenesis

Info

Publication number
ATE161541T1
ATE161541T1 AT94923258T AT94923258T ATE161541T1 AT E161541 T1 ATE161541 T1 AT E161541T1 AT 94923258 T AT94923258 T AT 94923258T AT 94923258 T AT94923258 T AT 94923258T AT E161541 T1 ATE161541 T1 AT E161541T1
Authority
AT
Austria
Prior art keywords
site
chemical
modified oligonucleotides
specific mutagenesis
molecule
Prior art date
Application number
AT94923258T
Other languages
English (en)
Inventor
Peter M Glazer
Pamela A Havre
Original Assignee
Univ Yale
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22176104&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE161541(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Univ Yale filed Critical Univ Yale
Application granted granted Critical
Publication of ATE161541T1 publication Critical patent/ATE161541T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/15—Nucleic acids forming more than 2 strands, e.g. TFOs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3511—Conjugate intercalating or cleaving agent

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Public Health (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Analytical Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Communicable Diseases (AREA)
  • Virology (AREA)
  • Oncology (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AT94923258T 1993-06-25 1994-06-24 Chemische-modifizierte oligonukleotide für site- spezifisches mutagenesis ATE161541T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US8308893A 1993-06-25 1993-06-25

Publications (1)

Publication Number Publication Date
ATE161541T1 true ATE161541T1 (de) 1998-01-15

Family

ID=22176104

Family Applications (1)

Application Number Title Priority Date Filing Date
AT94923258T ATE161541T1 (de) 1993-06-25 1994-06-24 Chemische-modifizierte oligonukleotide für site- spezifisches mutagenesis

Country Status (9)

Country Link
US (1) US7078389B2 (de)
EP (1) EP0705270B1 (de)
JP (1) JPH09503644A (de)
AT (1) ATE161541T1 (de)
AU (1) AU691194B2 (de)
CA (1) CA2166079C (de)
DE (1) DE69407590T2 (de)
ES (1) ES2113118T3 (de)
WO (1) WO1995001364A1 (de)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5849482A (en) * 1988-09-28 1998-12-15 Epoch Pharmaceuticals, Inc. Crosslinking oligonucleotides
US6136601A (en) * 1991-08-21 2000-10-24 Epoch Pharmaceuticals, Inc. Targeted mutagenesis in living cells using modified oligonucleotides
US5962426A (en) * 1993-06-25 1999-10-05 Yale University Triple-helix forming oligonucleotides for targeted mutagenesis
AU6043296A (en) * 1995-06-06 1996-12-24 Yale University Chemically modified oligonucleotide for site-directed mutage nesis
US7279463B2 (en) 1995-06-07 2007-10-09 Yale University Triple-helix forming oligonucleotides for targeted mutagenesis
US6124099A (en) * 1998-06-22 2000-09-26 The University Of Vermont And State Agricultural College Method for placing a photo-cross-linking agent at specific internal sites within the sequence of synthetic strands of ribonucleic acids
WO2001073001A2 (en) * 2000-03-24 2001-10-04 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Establishment of cellular manipulations which enhance oligo-mediated gene targeting
FR2823219B1 (fr) * 2001-04-10 2003-07-04 Pasteur Institut Mutants de la desoxycytidine kinase possedant une activite enzymatique elargie
US8658608B2 (en) 2005-11-23 2014-02-25 Yale University Modified triple-helix forming oligonucleotides for targeted mutagenesis
CA2693102C (en) 2007-07-31 2020-03-10 Verenium Corporation Tailored multi-site combinatorial assembly
US20110268810A1 (en) * 2009-11-02 2011-11-03 Yale University Polymeric materials loaded with mutagenic and recombinagenic nucleic acids
ES2966886T3 (es) 2014-05-09 2024-04-24 Univ Yale Partículas recubiertas con poliglicerol hiperramificado y métodos de elaboración y uso de las mismas
US11918695B2 (en) 2014-05-09 2024-03-05 Yale University Topical formulation of hyperbranched polymer-coated particles
US20200308590A1 (en) 2016-02-16 2020-10-01 Yale University Compositions and methods for treatment of cystic fibrosis
JP2019508037A (ja) 2016-02-16 2019-03-28 イェール ユニバーシティーＹａｌｅ Ｕｎｉｖｅｒｓｉｔ 標的化遺伝子編集を増強するための組成物およびその使用方法
WO2018187493A1 (en) 2017-04-04 2018-10-11 Yale University Compositions and methods for in utero delivery
US20210189431A1 (en) 2018-08-10 2021-06-24 Yale University Compositions and methods for embryonic gene editing in vitro
EP3844287B1 (de) 2018-08-31 2024-05-22 Yale University Zusammensetzungen und verfahren zur verbesserung der oligonukleotidbasierten geneditierung
CA3111186A1 (en) 2018-08-31 2020-03-05 Yale University Compositions and methods for enhancing triplex and nuclease-based gene editing
WO2020112195A1 (en) 2018-11-30 2020-06-04 Yale University Compositions, technologies and methods of using plerixafor to enhance gene editing
WO2020257779A1 (en) 2019-06-21 2020-12-24 Yale University Hydroxymethyl-modified gamma-pna compositions and methods of use thereof
WO2020257776A1 (en) 2019-06-21 2020-12-24 Yale University Peptide nucleic acid compositions with modified hoogsteen binding segments and methods of use thereof
US12496357B2 (en) 2019-07-31 2025-12-16 Yale University Compositions and methods for treating sickle cell disease
WO2021042060A1 (en) 2019-08-30 2021-03-04 Yale University Compositions and methods for delivery of nucleic acids to cells
US20220339294A1 (en) 2019-09-09 2022-10-27 Yale University Nanoparticles for selective tissue or cellular uptake

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4792562A (en) 1985-12-04 1988-12-20 Hoechst-Roussel Pharmaceuticals, Inc. N-(pyrrol-1-yl)pyridinamines having memory enhancing activity
EP0266099A3 (de) * 1986-10-28 1990-09-19 The Johns Hopkins University Alkyl- oder Arylphosphonat-Oligonukleosid, das sich mit Nukleinesäuren vernetzen kann oder das Nukleinsäure spalten kann
CA2006008C (en) * 1988-12-20 2000-02-15 Donald J. Kessler Method for making synthetic oligonucleotides which bind specifically to target sites on duplex dna molecules, by forming a colinear triplex, the synthetic oligonucleotides and methods of use
US5962426A (en) * 1993-06-25 1999-10-05 Yale University Triple-helix forming oligonucleotides for targeted mutagenesis

Also Published As

Publication number Publication date
WO1995001364A1 (en) 1995-01-12
US7078389B2 (en) 2006-07-18
DE69407590D1 (de) 1998-02-05
CA2166079A1 (en) 1995-01-12
AU691194B2 (en) 1998-05-14
CA2166079C (en) 2009-11-17
AU7318094A (en) 1995-01-24
DE69407590T2 (de) 1998-07-23
ES2113118T3 (es) 1998-04-16
JPH09503644A (ja) 1997-04-15
EP0705270A1 (de) 1996-04-10
EP0705270B1 (de) 1997-12-29
US20020028922A1 (en) 2002-03-07

Similar Documents

Publication Publication Date Title
DE69407590D1 (de) Chemische-modifizierte oligonukleotide für site-spezifisches mutagenesis
WO1996039195A3 (en) Chemically modified oligonucleotide for site-directed mutagenesis
EP0348458A4 (en) Dna and rna molecules stabilized by modifications of the 3'-terminal phosphodiester linkage and their use as nucleic acid probes and as therapeutic agents to block the expression of specifically targeted genes
Warpehoski et al. Sequence selectivity of DNA covalent modification
Wang et al. DNA supercoiling and its effects on DNA structure and function
DE69433626D1 (de) N-2 substituierte purine
Jones et al. Demography and phenology of gray whales and evaluation of whale-watching activities in Laguna San Ignacio, Baja California Sur, Mexico.
Li et al. Phosphorylating DNA with DNA
Braaten et al. Locations and contexts of sequences that hybridize to poly (dG-dT).(dC-dA) in mammalian ribosomal DNAs and two X-linked genes
Murray et al. The sequence specificity of bleomycin-induced DNA damage in intact cells.
DE69133329D1 (de) Markierte Oligonukleotide
ATE211769T1 (de) Oligonukleotide und verfahren zum nachweis von chlamydia trachomatis
DK0868530T3 (da) Kaskade-nucleinsyreamplificeringsreaktion
DE3578786D1 (de) Als sonden zum nachweis von schaedlichen, in chromosom-dns enthaltenen genen zu verwendende deoxyribonukleinsaeuremolekuele.
WO1999064582A3 (en) High-throughput screening of gene function using libraries for functional genomics applications
IL70765A0 (en) Methods and structures employing non-radioactive chemicallylabeled polynucleotides
DE69521517D1 (de) Prodrug-oligonukleotide
EP0439222A3 (en) Water-insoluble reagent, nucleic acid probe, test kit and diagnostic and purification methods
ZA853756B (en) Nucleic acid hybridization assay employing immobilized rna probes
Zito et al. Lead-catalyzed cleavage of ribonuclease P RNA as a probe for integrity of tertiary structure
DE69917636D1 (en) Polymerasesignalversuch
Kohwi Cationic metal-specific structures adopted by the poly (dG) region and the direct repeats in the chicken adult β A globin gene promoter
Kang et al. Metal ions cause the isomerization of certain intramolecular triplexes.
Barbe et al. Discriminated induction of SOS functions in Escherichia coli by alkylating agents
EP1090146A4 (de) Verwendung von dna-pk

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties