ATE164883T1 - Verfahren zur vorbereitung von doppel strangiger rna und seine anwendungen - Google Patents

Verfahren zur vorbereitung von doppel strangiger rna und seine anwendungen

Info

Publication number
ATE164883T1
ATE164883T1 AT93902352T AT93902352T ATE164883T1 AT E164883 T1 ATE164883 T1 AT E164883T1 AT 93902352 T AT93902352 T AT 93902352T AT 93902352 T AT93902352 T AT 93902352T AT E164883 T1 ATE164883 T1 AT E164883T1
Authority
AT
Austria
Prior art keywords
pct
double stranded
stranded rna
applications
date jun
Prior art date
Application number
AT93902352T
Other languages
English (en)
Inventor
Thierry Livache
Brigitte Fouque
Robert Teoule
Original Assignee
Cis Bio Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cis Bio Int filed Critical Cis Bio Int
Application granted granted Critical
Publication of ATE164883T1 publication Critical patent/ATE164883T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT93902352T 1991-12-18 1992-12-18 Verfahren zur vorbereitung von doppel strangiger rna und seine anwendungen ATE164883T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9115710A FR2685346B1 (fr) 1991-12-18 1991-12-18 Procede de preparation d'arn double-brin, et ses applications.

Publications (1)

Publication Number Publication Date
ATE164883T1 true ATE164883T1 (de) 1998-04-15

Family

ID=9420171

Family Applications (1)

Application Number Title Priority Date Filing Date
AT93902352T ATE164883T1 (de) 1991-12-18 1992-12-18 Verfahren zur vorbereitung von doppel strangiger rna und seine anwendungen

Country Status (7)

Country Link
US (1) US5795715A (de)
EP (1) EP0618966B1 (de)
JP (1) JP3515108B2 (de)
AT (1) ATE164883T1 (de)
DE (1) DE69225074T2 (de)
FR (1) FR2685346B1 (de)
WO (1) WO1993012229A1 (de)

Families Citing this family (100)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6605712B1 (en) * 1990-12-20 2003-08-12 Arch Development Corporation Gene transcription and ionizing radiation: methods and compositions
FR2706465B1 (fr) * 1993-06-11 1995-08-25 Heliosynthese Sa Procédé de production et d'extraction de superoxyde-dismutases thermostables à partir d'une culture de micro-organismes photosynthétiques.
US6458940B2 (en) * 1995-06-06 2002-10-01 Hybridon, Inc. HPV-specific oligonucleotides
US20030055240A1 (en) * 1995-06-06 2003-03-20 Roberts Peter C. HPV specific oligonucleotides
US6509149B2 (en) * 1995-06-06 2003-01-21 Hybridon, Inc. HPV-specific oligonucleotides
US6274369B1 (en) * 1996-02-02 2001-08-14 Invitrogen Corporation Method capable of increasing competency of bacterial cell transformation
US6506559B1 (en) 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA
CN101818145A (zh) * 1998-03-20 2010-09-01 联邦科学和工业研究组织 控制基因表达
AUPP249298A0 (en) 1998-03-20 1998-04-23 Ag-Gene Australia Limited Synthetic genes and genetic constructs comprising same I
AU776150B2 (en) * 1999-01-28 2004-08-26 Medical College Of Georgia Research Institute, Inc. Composition and method for (in vivo) and (in vitro) attenuation of gene expression using double stranded RNA
US20040138168A1 (en) * 1999-04-21 2004-07-15 Wyeth Methods and compositions for inhibiting the function of polynucleotide sequences
IL145778A0 (en) * 1999-04-21 2002-07-25 American Home Prod Methods and compositions for inhibiting the function of polynucleotide sequences
US7056661B2 (en) * 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6423885B1 (en) 1999-08-13 2002-07-23 Commonwealth Scientific And Industrial Research Organization (Csiro) Methods for obtaining modified phenotypes in plant cells
EP1235842A4 (de) * 1999-10-15 2003-04-23 Univ Massachusetts Gene des rns-interferenzweges als hilfsmittel der gezielten gentischen interferenz
US6326193B1 (en) 1999-11-05 2001-12-04 Cambria Biosciences, Llc Insect control agent
KR20080023768A (ko) 2000-03-30 2008-03-14 화이트헤드 인스티튜트 포 바이오메디칼 리서치 Rna 간섭의 rna 서열 특이적인 매개체
US7700359B2 (en) * 2000-06-02 2010-04-20 Novartis Vaccines And Diagnostics, Inc. Gene products differentially expressed in cancerous cells
US6465217B1 (en) * 2000-07-05 2002-10-15 Paradigm Genetics, Inc. Methods and compositions for the modulation of chorismate synthase and chorismate mutase expression or activity in plants
US20080242627A1 (en) * 2000-08-02 2008-10-02 University Of Southern California Novel rna interference methods using dna-rna duplex constructs
US20020173478A1 (en) * 2000-11-14 2002-11-21 The Trustees Of The University Of Pennsylvania Post-transcriptional gene silencing by RNAi in mammalian cells
CN100523215C (zh) 2000-12-01 2009-08-05 马普科技促进协会 介导rna干涉的小rna分子
US20020132257A1 (en) * 2001-01-31 2002-09-19 Tony Giordano Use of post-transcriptional gene silencing for identifying nucleic acid sequences that modulate the function of a cell
US8034791B2 (en) 2001-04-06 2011-10-11 The University Of Chicago Activation of Egr-1 promoter by DNA damaging chemotherapeutics
US20040242523A1 (en) * 2003-03-06 2004-12-02 Ana-Farber Cancer Institue And The Univiersity Of Chicago Chemo-inducible cancer gene therapy
ATE541937T1 (de) * 2001-04-06 2012-02-15 Univ Chicago Chemotherapeutische einleitung der egr-1-promoter-aktivität in gentherapie
AU2003303395A1 (en) * 2001-05-22 2004-07-22 Dahl, Gary, A. Target-dependent transcription using deletion mutants of n4 rna polymerase
AUPR621501A0 (en) 2001-07-06 2001-08-02 Commonwealth Scientific And Industrial Research Organisation Delivery of ds rna
TWI335938B (en) * 2001-08-15 2011-01-11 Rna replication and amplification
EP1451325B1 (de) 2001-11-09 2011-05-18 BASF Plant Science GmbH Mit stress assoziierte transkriptionsfaktor-spezifische polypeptide und verfahren zur verwendung in pflanzen
ES2335089T3 (es) 2001-11-09 2010-03-22 Basf Plant Science Gmbh Polipeptidos de proteina quinasa relacionados con el estres y metodos de uso en las plantas.
EP1472375B1 (de) 2002-01-08 2009-04-01 Novartis Vaccines and Diagnostics, Inc. In kanzerösen mammazellen differentiell exprimierte genprodukte und ihre verwendungsverfahren
US20030166282A1 (en) * 2002-02-01 2003-09-04 David Brown High potency siRNAS for reducing the expression of target genes
EP1478656B1 (de) 2002-02-01 2009-09-16 Life Technologies Corporation Oligonukleotidzusammensetzungen mit verbesserter effizienz
US20060009409A1 (en) 2002-02-01 2006-01-12 Woolf Tod M Double-stranded oligonucleotides
US20040248094A1 (en) * 2002-06-12 2004-12-09 Ford Lance P. Methods and compositions relating to labeled RNA molecules that reduce gene expression
WO2003106630A2 (en) * 2002-06-12 2003-12-24 Ambion, Inc. Methods and compositions relating to polypeptides with rnase iii domains that mediate rna interference
WO2004013288A2 (en) 2002-08-01 2004-02-12 City Of Hope Methods and kits for synthesis of sirna expression cassettes
US7618948B2 (en) 2002-11-26 2009-11-17 Medtronic, Inc. Devices, systems and methods for improving and/or cognitive function through brain delivery of siRNA
US7829694B2 (en) * 2002-11-26 2010-11-09 Medtronic, Inc. Treatment of neurodegenerative disease through intracranial delivery of siRNA
US6852494B2 (en) * 2003-01-10 2005-02-08 Linden Technologies, Inc. Nucleic acid amplification
EP1604022A2 (de) * 2003-03-06 2005-12-14 Oligo Engine, Inc. Veränderung der genexpression mit dns-rns hybriden
CN103131673A (zh) 2003-04-15 2013-06-05 巴斯福植物科学有限公司 编码非生物胁迫反应相关蛋白质的核酸序列,以及提高对环境胁迫耐性的植物和植物细胞
BRPI0410787A (pt) * 2003-05-21 2006-06-20 Univ Texas inibição da proteìna cinase c-mu (pkd) como um tratamento para hipertrofia cardìaca e insuficiência cardìaca
WO2005095622A2 (en) * 2004-03-26 2005-10-13 Van Andel Research Institute c-met siRNA ADENOVIRUS VECTORS INHIBIT CANCER CELL GROWTH, INVASION AND TUMORIGENICITY
WO2005103254A1 (en) * 2004-04-22 2005-11-03 Yissum Research Development Company Of The Hebrew University Of Jerusalem UNIVERSAL TARGET SEQUENCES FOR siRNA GENE SILENCING
NL1026335C2 (nl) * 2004-06-04 2005-12-06 Univ Delft Tech Werkwijze voor het maken van een dubbelstrengs polyribonucleotidenvolgorde met overhangend uiteinde, alsmede een werkwijze voor het vormen van een dubbelstrengs polynucleotidenconstruct en een toepassing.
WO2006032708A2 (en) 2004-09-24 2006-03-30 Basf Plant Science Gmbh Nucleic acid sequences encoding proteins associated with abiotic stress response and plant cells and plants with increased tolerance to environmental stress
EP1794304B1 (de) 2004-09-24 2013-06-19 BASF Plant Science GmbH Pflanzenzellen und pflanzen mit erhöhter umweltstresstoleranz
US20060142228A1 (en) * 2004-12-23 2006-06-29 Ambion, Inc. Methods and compositions concerning siRNA's as mediators of RNA interference
DE202005004135U1 (de) * 2005-03-11 2005-05-19 Klocke Verpackungs-Service Gmbh Mehrkomponentenverpackung mit Applikator
CA2610644A1 (en) * 2005-05-31 2006-12-07 Devgen Nv Rnai for control of insects and arachnids
CA2612016A1 (en) 2005-06-17 2006-12-21 Basf Plant Science Gmbh Lecitin-like protein kinase stress-related polypeptides and methods of use in plants
CN101228277B (zh) 2005-07-18 2013-11-27 巴斯福植物科学有限公司 过表达accdp基因的植物中的产量增加
US20100162434A1 (en) 2005-07-18 2010-06-24 Basf Plant Science Gmbh Yield Increase in Plants Overexpressing the SHSRP Genes
CA2619411A1 (en) 2005-08-12 2007-02-22 Basf Plant Science Gmbh Nucleic acid sequences encoding proteins associated with abiotic stress response and plant cells and plants with increased tolerance to environmental stress
WO2007047408A2 (en) * 2005-10-12 2007-04-26 Pathologica, Llc. Promac signature application
US8808698B2 (en) * 2006-02-03 2014-08-19 The Regents Of The University Of California Methods for inhibition of lymphangiogenesis and tumor metastasis
EP2343377A3 (de) 2006-03-24 2011-11-16 BASF Plant Science GmbH Mit der abiotischen Stressreaktion assoziierte Proteine und Homologe
JP2010500011A (ja) 2006-08-08 2010-01-07 ガンサー ハートマン 5’リン酸オリゴヌクレオチドの構造および使用
BRPI0719824A2 (pt) 2006-10-13 2014-06-03 Basf Plant Science Gmbh Método para produzir uma célula vegetal transgênica, uma planta ou uma parte da mesma, célula hospedeira transformada, célula vegetal transgênica, uma planta ou uma parte da mesma, semente, tecido vegetal, material de propagação, material colhido ou uma planta, e, uso de uma molécula de ácido nucleico.
KR20090119910A (ko) 2007-02-16 2009-11-20 바스프 플랜트 사이언스 게엠베하 외떡잎 식물에서 배-특이적 발현의 조절을 위한 핵산 서열
US20100251416A1 (en) 2007-05-22 2010-09-30 Basf Plant Science Gmbh Plants with increased tolerance and/or resistance to environmental stress and increased biomass production
EP2152890A1 (de) * 2007-05-23 2010-02-17 MannKind Corporation Multicistronische vektoren und verfahren zu deren konstruktion
BRPI0817005A2 (pt) 2007-09-18 2019-09-24 Basf Plant Science Gmbh método para produzir uma célula de planta transgênica, uma planta ou uma parte da mesma com rendimento aumentado, molécula de ácido nucleico, construção de ácido nucleico, vetor, célula hospedeira, processo para produzir um polipeptídeo, polipeptídeo, anticorpo, núcleo de célula de planta transgênica, célula de planta, tecido de planta, material de propagação, material colhido, planta ou parte da mesma, semente, processo para identificar um composto, método para produzir uma composição agrícola, composição, e, uso de uma molécula de ácido nucleico
EP2594645A3 (de) 2007-09-21 2013-11-06 BASF Plant Science GmbH Pflanzen mit erhöhtem Ertrag
BRPI0821748A2 (pt) 2007-12-19 2019-09-24 Basf Plant Science Gmbh método para produzir uma planta com rendimento aumentado, molécula isolada de ácido nucleico, construção de ácido nucleico, vetor, processo para produzir um polipeptídeo, polipeptídeo, anticorpo, núcleo de célula de planta, célula de planta, tecido de planta, material de propagação, semente, pólen, progênie, ou uma parte de planta, ou uma planta com rendimento aumento, processo para a indentificação de um composto, método para a produção de uma composição agrícola, composição, polipeptídeo ou molécula de ácido nucleico, uso dos ácidos nucléicos, e, método para a indentificação de uma planta com um rendimento aumentado
EP2245464B1 (de) 2008-01-25 2016-12-14 MultiVir Inc. P53-biomarker
WO2009097508A2 (en) 2008-01-30 2009-08-06 Mcanulty, Jonathan Methods and compositions for wound healing
AU2009218478A1 (en) * 2008-02-27 2009-09-03 Basf Plant Science Gmbh Plants with increased yield
US9738680B2 (en) 2008-05-21 2017-08-22 Rheinische Friedrich-Wilhelms-Universität Bonn 5′ triphosphate oligonucleotide with blunt end and uses thereof
CA2734347A1 (en) 2008-08-19 2010-02-25 Basf Plant Science Gmbh Plants with increased yield by increasing or generating one or more activities in a plant or a part thereof
EP2337853A1 (de) 2008-09-23 2011-06-29 BASF Plant Science Company GmbH Pflanzen mit erhöhtem ertrag (lt)
DE112009002577T5 (de) 2008-10-23 2012-06-21 Basf Plant Science Gmbh Pflanzen mit erhöhtem Ertrag (NUE)
WO2010046471A2 (en) 2008-10-23 2010-04-29 Basf Plant Science Gmbh A method for producing a transgenic cell with increased gamma-aminobutyric acid (gaba) content
EP2186822A1 (de) 2008-11-07 2010-05-19 Sanofi-Aventis Verwendung von Inhibitoren der Plac8-Aktivität zur Modulation von Adipogenese
EP2236607A1 (de) 2009-04-02 2010-10-06 Sanofi-Aventis Verwendung von Hemmern der St3Gla6-Aktivität zur Adipogenesemodulation
EP2253705A1 (de) 2009-05-20 2010-11-24 Sanofi-Aventis Verwendung von Hemmern der Zdhhc2-Aktivität zur Adipogenesemodulation
DE112010003032T5 (de) 2009-07-23 2012-08-02 Basf Plant Science Company Gmbh Pflanzen mit erhöhtem Ertrag
US9409983B2 (en) 2009-07-23 2016-08-09 The Board Of Trustess Of The University Of Illinois Methods and compositions involving PBEF inhibitors for lung inflammation conditions and diseases
EP2308892A1 (de) 2009-10-01 2011-04-13 Sanofi-Aventis Verwendung von siRNA gegen Sipa1l1 zur Reduzierung von Lipogenese
MX2012005719A (es) 2009-11-17 2012-07-30 Basf Plant Science Co Gmbh Plantas con rendimiento aumentado.
WO2016187217A2 (en) 2015-05-18 2016-11-24 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for treating aging-associated impairments
EP2531261B1 (de) 2010-02-01 2016-08-31 The Board of Trustees of the Leland Stanford Junior University Verfahren zur diagnose und behandlung von nicht-insulinabhängiger diabetes mellitus
WO2013005211A2 (en) 2011-07-05 2013-01-10 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Boron complexing plant materials and uses thereof cross-reference to related applications
WO2013036799A2 (en) 2011-09-09 2013-03-14 Fred Hutchinson Cancer Research Center Methods and compositions involving nkg2d inhibitors and cancer
WO2014045126A2 (en) 2012-09-18 2014-03-27 Uti Limited Partnership Treatment of pain by inhibition of usp5 de-ubiquitinase
EP2712870A1 (de) 2012-09-27 2014-04-02 Rheinische Friedrich-Wilhelms-Universität Bonn Neuartige RIG-I-Liganden und Herstellungsverfahren dafür
WO2014074503A1 (en) 2012-11-06 2014-05-15 Imbed Biosciences, Inc. Methods and compositions for wound healing
EP2961853B1 (de) 2013-02-28 2018-09-19 The Board of Regents of The University of Texas System Verfahren zur klassifizierung einer krebserkrankung als anfälligkeit für tmepai-gerichtete therapien und zur heilung solcher krebserkrankungen
US20170002064A1 (en) 2013-11-08 2017-01-05 The Board Of Regents Of The University Of Texas System Vh4 antibodies against gray matter neuron and astrocyte
US10265372B2 (en) 2014-08-12 2019-04-23 The Regents Of The University Of California Molecular composition for enhancing and rejuvenating maintenance and repair of mammalian tissues
US10278986B2 (en) 2014-08-14 2019-05-07 The Regents Of The University Of Colorado, A Body Corporate Antibody-siRNA conjugates and uses therefor
EP3144014A1 (de) 2015-09-21 2017-03-22 Commissariat A L'energie Atomique Et Aux Energies Alternatives Synthetische tödliche arzneimittelkombination zur behandlung von nierenzellenkarzinom
KR102637746B1 (ko) 2016-07-29 2024-02-16 임베드 바이오사이언시스 아이엔씨. 상처 치유를 위한 방법 및 조성물
CA3053392A1 (en) 2017-02-28 2018-09-07 The Board Of Trustees Of The Leland Stanford Junior University Antifibrotic activity of cd47 blockade
CN119506283A (zh) 2017-09-15 2025-02-25 联邦科学技术研究组织 Rna分子
CN113614535B (zh) 2018-10-31 2024-08-16 亚利桑那大学评议会 用于辐射诱导的肺损伤的生物标志物和使用方法
US20230266325A1 (en) 2020-06-30 2023-08-24 Lunglife Ai, Inc. Methods for detecting lung cancer
EP4532772A1 (de) 2022-05-24 2025-04-09 Lunglife Ai, Inc. Verfahren zum nachweis zirkulierender genetisch abnormaler zellen

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4766072A (en) * 1985-07-17 1988-08-23 Promega Corporation Vectors for in vitro production of RNA copies of either strand of a cloned DNA sequence
EP0473576A4 (en) * 1989-05-19 1993-03-10 Hem Research, Inc. Short therapeutic dsrna of defined structure
ATE184321T1 (de) * 1990-01-10 1999-09-15 Chiron Diagnostics Corp Dns-abhängige rns-polymerase-transkripte als reportermoleküle zur signalverstärkung in nukleinsäure-hybridisierungsuntersuchungen

Also Published As

Publication number Publication date
DE69225074T2 (de) 1998-10-29
JP3515108B2 (ja) 2004-04-05
EP0618966A1 (de) 1994-10-12
DE69225074D1 (de) 1998-05-14
US5795715A (en) 1998-08-18
FR2685346B1 (fr) 1994-02-11
WO1993012229A1 (fr) 1993-06-24
EP0618966B1 (de) 1998-04-08
JPH07505762A (ja) 1995-06-29
FR2685346A1 (fr) 1993-06-25

Similar Documents

Publication Publication Date Title
DE69225074D1 (de) Verfahren zur vorbereitung von doppel strangiger rna und seine anwendungen
AU692403B2 (en) Analysis of gene expression by display of 3'-end restriction fragments of cdnas
EP0777747B1 (de) Nukleotid-sequenzierungsmethode
DE69307503D1 (de) Chemisches verfahren zur analyse von dns-sequenzen
DE68925717D1 (de) Verfahren und Reagenzkombination zur Bestimmung von Nukleotidsequenzen
EP1757694A3 (de) Prostata-spezifisches Membranantigen
ATE73502T1 (de) Verfahren und ausstattung zur bestimmung von spezifischen nukleotid-variationen und genetischen polymorphismen in nukleinsaeuren.
ATE204332T1 (de) Positionelle sequenzierung durch hybridisierung
EP0969102A3 (de) Primer, Sätze und Restriktions Fragmente und dessen Benutzung in selektive Restriktionsfragmentenamplifikation
EP0359789A1 (de) Verstärkung und nachweis von nukleinsäuresequenzen.
DE60220025D1 (de) Methoden und zusammensetzungen zur vervielfältigung von rna sequenzen
DE69428252D1 (de) Verfahren zur Verbesserung der Nukleinsäureamplifizierung
WO1993016094A3 (en) Applications of fluorescent n-nucleosides and fluorescent structural analogs of n-nucleosides
AU1022488A (en) T7 dna polymerase
WO1998026093A3 (en) Fluorescent nucleotide analog hairpin formation for detection of nucleic acid hybridization
DE60116651D1 (de) Methode zur amplifizierung und möglicher charakterisierung von nukleinsäuren
ATE542917T1 (de) Zusammensetzungen, verfahren und kits zur bestimmung des vorhandenseins von trichomonas vaginalis in einer testprobe
CA2264967A1 (en) Method of dna sequencing
DE69815370D1 (de) Fluoreszend-konjugaten von nukleosiden und von nukleotiden, ihrer verfahren zur herstellung und ihrer verwendungen
WO2001088196A3 (en) Sequence based screening
WO2000026406A1 (en) METHOD FOR INDEXING AND DETERMINING THE RELATIVE CONCENTRATION OF EXPRESSED MESSENGER RNAs
WO2002072881A3 (en) Method for the amplification and detection of hbv dna using a transcription based amplification
EP0286958A3 (de) Verfahren und Mittel zur Bestimmung von Nucleinsäuren
WO2005059505A3 (es) Método y kit para genotipificación de hla-b27 basados en la pcr en tiempo real
Palmirotta et al. Origin and gender determination of dried blood on a statue of the Virgin Mary