ATE167193T1 - Waessrige mehrphasige isolierung eines polypeptids - Google Patents

Waessrige mehrphasige isolierung eines polypeptids

Info

Publication number
ATE167193T1
ATE167193T1 AT94925830T AT94925830T ATE167193T1 AT E167193 T1 ATE167193 T1 AT E167193T1 AT 94925830 T AT94925830 T AT 94925830T AT 94925830 T AT94925830 T AT 94925830T AT E167193 T1 ATE167193 T1 AT E167193T1
Authority
AT
Austria
Prior art keywords
polypeptide
aqueous
enriched
phases
cells
Prior art date
Application number
AT94925830T
Other languages
English (en)
Inventor
Stuart Builder
Roger Hart
Philip Lester
John Ogez
David Reifsnyder
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Application granted granted Critical
Publication of ATE167193T1 publication Critical patent/ATE167193T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/803—Physical recovery methods, e.g. chromatography, grinding
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/804—Single cell protein
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/808—Materials and products related to genetic engineering or hybrid or fused cell technology, e.g. hybridoma, monoclonal products

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Endocrinology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Diabetes (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT94925830T 1993-08-20 1994-08-10 Waessrige mehrphasige isolierung eines polypeptids ATE167193T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/110,663 US5407810A (en) 1993-08-20 1993-08-20 Aqueous multiple-phase isolation of polypeptide

Publications (1)

Publication Number Publication Date
ATE167193T1 true ATE167193T1 (de) 1998-06-15

Family

ID=22334217

Family Applications (1)

Application Number Title Priority Date Filing Date
AT94925830T ATE167193T1 (de) 1993-08-20 1994-08-10 Waessrige mehrphasige isolierung eines polypeptids

Country Status (11)

Country Link
US (3) US5407810A (de)
EP (1) EP0714403B1 (de)
JP (1) JP3646195B2 (de)
AT (1) ATE167193T1 (de)
AU (1) AU673624B2 (de)
CA (1) CA2167910C (de)
DE (1) DE69411015T2 (de)
DK (1) DK0714403T3 (de)
ES (1) ES2119222T3 (de)
GR (1) GR3027656T3 (de)
WO (1) WO1995006059A1 (de)

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WO1996004556A1 (en) * 1994-08-01 1996-02-15 Hsu James T Lipoprotein cholesterol assays
EP0779927A1 (de) * 1994-09-08 1997-06-25 Chiron Corporation Methode zur gesteigerten herstellung von insulin-ähnlichem wachstumsfaktor
GB9618952D0 (en) 1996-09-11 1996-10-23 Sandoz Ltd Process
US6756484B1 (en) 1995-04-14 2004-06-29 Cephalon, Inc. IGF-I purification process
US5650496A (en) 1995-04-14 1997-07-22 Cephalon, Inc. IGF-I purification process
US5789213A (en) * 1995-05-11 1998-08-04 Health Research Inc. Method and compositions for high efficiency loading, transfection and fusion of cells by electric pulses
JP2002514890A (ja) * 1995-06-07 2002-05-21 カイロン コーポレイション 酵母宿主から真性のｉｇｆを精製するための方法
ATE301675T1 (de) * 1995-06-07 2005-08-15 Chiron Corp Methode zur lösung, reinigung und rückfaltung von protein
US7071313B1 (en) 1995-06-07 2006-07-04 Cephalon, Inc. Methods of purifying authentic IGF from yeast hosts
US7193042B1 (en) 1995-06-07 2007-03-20 Chiron Corporation Methods for purifying authentic IGF from yeast hosts
US5945515A (en) * 1995-07-31 1999-08-31 Chomczynski; Piotr Product and process for isolating DNA, RNA and proteins
SE9504019D0 (sv) * 1995-11-13 1995-11-13 Pharmacia Ab Method for production of proteins
US6136960A (en) * 1996-04-19 2000-10-24 Boris Y. Zaslavsky Method for evaluation of the ratio of amounts of biomolecules or their sub-populations in a mixture
US5734024A (en) * 1996-04-19 1998-03-31 Boris Y. Zaslavsky Method for determining the biological activity of recombinant human growth hormone
IT1291362B1 (it) * 1997-05-13 1999-01-07 Vectorpharma Int Composizioni farmaceutiche multicomponente bifasiche contenenti sostanze atte a modificare la partizione dei principi attivi
US20020052026A1 (en) * 1997-10-08 2002-05-02 Steven M. Vicik Methods of refolding proteins
US6265542B1 (en) 1997-10-24 2001-07-24 Genentech, Inc. Purification of molecules
AU5209399A (en) 1998-07-10 2000-02-01 Cornell Research Foundation Inc. Recombinant constructs and systems for secretion of proteins via type iii secretion systems
ES2297947T3 (es) 1998-09-22 2008-05-01 Genentech, Inc. Ucp4.
US7342102B2 (en) 1998-11-30 2008-03-11 Genentech, Inc. Uncoupling protein 5 (UCP5)
AU774760B2 (en) * 1998-11-30 2004-07-08 Genentech Inc. Ucp5
WO2000040610A1 (fr) * 1999-01-07 2000-07-13 Takeda Chemical Industries, Ltd. Procede de production de proteines activees
US6437101B1 (en) 1999-05-07 2002-08-20 Akzo Nobel N.V. Methods for protein purification using aqueous two-phase extraction
US7229789B1 (en) * 2001-01-19 2007-06-12 N.V. Organon Methods and compositions for extracting proteins from cells
EP1772464B1 (de) 2001-02-09 2009-08-12 Genentech, Inc. Verfahren zur Identifizierung von indirekten IGF-1-Agonisten
EP1314739A1 (de) * 2001-11-22 2003-05-28 Bayer Ag Verfahren zur Renaturierung rekombinanter, Disulfid-enthaltender Proteine bei hohen Proteinkonzentrationen in Gegenwart von Aminen
US7998705B2 (en) * 2002-08-06 2011-08-16 FUJIFILM Diosynth Biotechnologies U.S.A., Inc Increased dynamic binding capacity in ion exchange chromatography by addition of polyethylene glycol
US6924124B1 (en) * 2002-08-23 2005-08-02 Immunex Corporation Feeding strategies for cell culture
JP4836795B2 (ja) * 2003-05-19 2011-12-14 ブランデイズ ユニバーシティー 核酸プロセシング方法、キット、及び装置
US8193312B2 (en) 2004-01-28 2012-06-05 The Regents Of The University Of California Bone morphogenic protein binding peptide
WO2005072403A2 (en) 2004-01-28 2005-08-11 The Regents Of The University Of California Bone morphogenic protein binding peptide
US8415302B2 (en) 2004-01-28 2013-04-09 The Regents Of The University Of California Surgical applications for BMP binding protein
DE102004015965A1 (de) * 2004-04-01 2005-10-20 Aventis Pharma Gmbh Verfahren zur Gewinnung von Insulinen durch verbesserte Luftbegasung der Faltung
AU2005259269B2 (en) 2004-06-29 2010-12-09 Ares Trading S.A. Process for the purification of IL-18 binding protein
EP1853283B1 (de) * 2004-11-29 2015-09-16 The Regents of The University of California Aktivierung der extraktion einer demineralisierten knochenmatrix
CA2618068C (en) * 2005-08-05 2016-02-16 Amgen Inc. Stable aqueous protein or antibody pharmaceutical formulations and their preparation
US20070048786A1 (en) * 2005-09-01 2007-03-01 Arnon Chait Systems and methods for fractionation of protein mixtures
MX371080B (es) 2009-06-23 2020-01-15 Univ California Aumento de retención de bmp.
US10377999B2 (en) * 2014-09-26 2019-08-13 Xyleco, Inc. Solubilized enzyme and uses thereof
HUE056847T2 (hu) * 2014-10-01 2022-03-28 Impossible Foods Inc Eljárások nem denaturált fehérjék kivonására és tisztítására
JP2016172707A (ja) * 2015-03-18 2016-09-29 株式会社ＵｎｉＢｉｏ 植物体内で発現させた上皮細胞増殖因子の精製品の製造方法
US11051532B2 (en) 2017-09-22 2021-07-06 Impossible Foods Inc. Methods for purifying protein
US12011016B2 (en) 2020-09-14 2024-06-18 Impossible Foods Inc. Protein methods and compositions

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Also Published As

Publication number Publication date
US5695958A (en) 1997-12-09
US5407810A (en) 1995-04-18
EP0714403A1 (de) 1996-06-05
DK0714403T3 (da) 1999-03-22
DE69411015D1 (de) 1998-07-16
EP0714403B1 (de) 1998-06-10
ES2119222T3 (es) 1998-10-01
JP3646195B2 (ja) 2005-05-11
US5723310A (en) 1998-03-03
WO1995006059A1 (en) 1995-03-02
GR3027656T3 (en) 1998-11-30
JPH09501931A (ja) 1997-02-25
AU7561694A (en) 1995-03-21
AU673624B2 (en) 1996-11-14
DE69411015T2 (de) 1998-10-22
CA2167910C (en) 2009-10-06
CA2167910A1 (en) 1995-03-02

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