ATE170980T1 - Bestimmung von analyten durch übertragung von fluoreszenzenergie - Google Patents

Bestimmung von analyten durch übertragung von fluoreszenzenergie

Info

Publication number
ATE170980T1
ATE170980T1 AT91913255T AT91913255T ATE170980T1 AT E170980 T1 ATE170980 T1 AT E170980T1 AT 91913255 T AT91913255 T AT 91913255T AT 91913255 T AT91913255 T AT 91913255T AT E170980 T1 ATE170980 T1 AT E170980T1
Authority
AT
Austria
Prior art keywords
concentration
analytes
fluorochromes
fluorochrome
camp
Prior art date
Application number
AT91913255T
Other languages
English (en)
Inventor
Roger Y Tsien
Susan S Taylor
Stephen R Adams
Ying Ji
Original Assignee
Univ California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ California filed Critical Univ California
Application granted granted Critical
Publication of ATE170980T1 publication Critical patent/ATE170980T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/80—Fluorescent dyes, e.g. rhodamine

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Information Transfer Systems (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
  • Holo Graphy (AREA)
  • Two-Way Televisions, Distribution Of Moving Picture Or The Like (AREA)
  • Studio Devices (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT91913255T 1990-07-02 1991-07-01 Bestimmung von analyten durch übertragung von fluoreszenzenergie ATE170980T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US54799090A 1990-07-02 1990-07-02

Publications (1)

Publication Number Publication Date
ATE170980T1 true ATE170980T1 (de) 1998-09-15

Family

ID=24186980

Family Applications (1)

Application Number Title Priority Date Filing Date
AT91913255T ATE170980T1 (de) 1990-07-02 1991-07-01 Bestimmung von analyten durch übertragung von fluoreszenzenergie

Country Status (7)

Country Link
US (1) US5439797A (de)
EP (1) EP0537270B1 (de)
JP (1) JP3208486B2 (de)
AT (1) ATE170980T1 (de)
DE (1) DE69130171T2 (de)
DK (1) DK0537270T3 (de)
WO (1) WO1992000388A1 (de)

Families Citing this family (83)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9310978D0 (en) 1993-05-27 1993-07-14 Zeneca Ltd Compounds
US6482655B1 (en) * 1993-07-23 2002-11-19 University Of Utah Research Foundation Immunoassay procedure utilizing fluorogenic tracer antigens
US5861124A (en) * 1993-07-15 1999-01-19 Hamamatsu Photonics Kk Method and apparatus for detecting denaturation of nucleic acid
US6576419B1 (en) 1993-07-23 2003-06-10 University Of Utah Research Foundation Assay procedure using fluorogenic tracers
US6028190A (en) 1994-02-01 2000-02-22 The Regents Of The University Of California Probes labeled with energy transfer coupled dyes
US5625048A (en) * 1994-11-10 1997-04-29 The Regents Of The University Of California Modified green fluorescent proteins
US6008373A (en) 1995-06-07 1999-12-28 Carnegie Mellon University Fluorescent labeling complexes with large stokes shift formed by coupling together cyanine and other fluorochromes capable of resonance energy transfer
US6766183B2 (en) 1995-11-22 2004-07-20 Medtronic Minimed, Inc. Long wave fluorophore sensor compounds and other fluorescent sensor compounds in polymers
US7388092B2 (en) 1996-05-03 2008-06-17 Applera Corporation Oligonucleotides and analogs labeled with energy transfer dyes
US5945526A (en) 1996-05-03 1999-08-31 Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US7825237B2 (en) 1996-05-03 2010-11-02 Applied Biosystems, Llc Oligonucleotides and analogs labeled with energy transfer dyes
US5814449A (en) * 1996-05-28 1998-09-29 University Of Pittsburgh Homogenous affinity assay for quantitative drug and metabolite determination
US6416960B1 (en) 1996-08-08 2002-07-09 Prolume, Ltd. Detection and visualization of neoplastic tissues and other tissues
US6458547B1 (en) 1996-12-12 2002-10-01 Prolume, Ltd. Apparatus and method for detecting and identifying infectious agents
US6197928B1 (en) * 1997-03-14 2001-03-06 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes
US5998204A (en) * 1997-03-14 1999-12-07 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes
DE69840146D1 (de) * 1997-03-14 2008-12-04 Univ California Fluoreszenzprotein-sensoren zur erkennung von analyten
FR2764387B1 (fr) 1997-06-05 1999-07-23 Centre Nat Rech Scient Utilisation d'une proteine fluorescente pour la detection d'interactions entre une proteine cible et son ligand
US7105307B2 (en) 1997-08-30 2006-09-12 Cyclacel, Ltd. Compositions and methods for screening for modulators of enzymatic activity
GB9718358D0 (en) * 1997-08-30 1997-11-05 Univ Leeds Chemical modification
FR2768817B1 (fr) * 1997-09-19 1999-12-10 Cis Bio Int Methode homogene pour la detection et/ou la determination de l'activite phosphorylante d'un materiel biologique
US6485901B1 (en) 1997-10-27 2002-11-26 Boston Probes, Inc. Methods, kits and compositions pertaining to linear beacons
EP1025120B1 (de) * 1997-10-27 2010-08-18 Boston Probes, Inc. Sich auf "pna molecular beacons" beziehende verfahren, testsätze und zusammensetzungen
WO1999049293A2 (en) 1998-03-24 1999-09-30 Boston Probes, Inc. Methods, kits and compositions pertaining to detection complexes
AU765703B2 (en) 1998-03-27 2003-09-25 Bruce J. Bryan Luciferases, fluorescent proteins, nucleic acids encoding the luciferases and fluorescent proteins and the use thereof in diagnostics, high throughput screening and novelty items
DE60015319T2 (de) * 1999-02-25 2005-10-27 Cyclacel Ltd. Test zur gleichzeitigen Messung von verschiedenen enzymischen Aktivitäten
GB9904401D0 (en) * 1999-02-25 1999-04-21 Fluorescience Ltd Assay method
US6828106B2 (en) * 1999-02-26 2004-12-07 Cyclacel Limited Methods and compositions using coiled binding partners
US7166475B2 (en) * 1999-02-26 2007-01-23 Cyclacel Ltd. Compositions and methods for monitoring the modification state of a pair of polypeptides
US6670144B1 (en) 1999-02-26 2003-12-30 Cyclacel, Ltd. Compositions and methods for monitoring the phosphorylation of natural binding partners
US6656696B2 (en) 1999-02-26 2003-12-02 Cyclacel Compositions and methods for monitoring the phosphorylation of natural binding partners
US6972182B1 (en) * 1999-02-26 2005-12-06 Cyclacel, Ltd. Methods and compositions using coiled binding partners
US7060793B2 (en) * 1999-05-21 2006-06-13 The Regents Of The University Of California Circularly permuted fluorescent protein indicators
US6699687B1 (en) 1999-05-21 2004-03-02 The Regents Of The University Of California Circularly permuted fluorescent protein indicators
US6469154B1 (en) 1999-05-21 2002-10-22 The Regents Of The University Of California Fluorescent protein indicators
AU778150B2 (en) * 1999-05-28 2004-11-18 Gendaq Limited Molecular switches
US6573059B1 (en) 1999-06-04 2003-06-03 Rmf Dictagene S.A. Use of the regulatory subunit of the camp dependent protein kinase (PKA) from dictyostelium for camp measurements
CA2380238A1 (en) * 1999-07-27 2001-02-01 University Of Utah Research Foundation Homogeneous fluorescence method for assaying structural modifications of biomolecules
US6358684B1 (en) 1999-08-27 2002-03-19 Pe Corporation UV excitable fluorescent energy transfer dyes
US7332275B2 (en) 1999-10-13 2008-02-19 Sequenom, Inc. Methods for detecting methylated nucleotides
EP1305412A2 (de) 1999-10-14 2003-05-02 Clontech Laboratories Inc. Chromo/fluoroproteine aus anthozoa und verwendung der gleichen
AU2137101A (en) * 1999-12-22 2001-07-03 Biosignal Packard Inc. A bioluminescence resonance energy transfer (bret) fusion molecule and method ofuse
WO2001053479A2 (en) * 2000-01-24 2001-07-26 Sangamo Biosciences, Inc. Molecular switches ii
US7060506B2 (en) 2000-01-31 2006-06-13 Cyclacel, Ltd. Compositions and methods for monitoring the modification of modification dependent binding partner polypeptides
US7109315B2 (en) * 2000-03-15 2006-09-19 Bruce J. Bryan Renilla reniformis fluorescent proteins, nucleic acids encoding the fluorescent proteins and the use thereof in diagnostics, high throughput screening and novelty items
US6808874B2 (en) 2000-06-13 2004-10-26 Cyclacel Ltd. Methods of monitoring enzyme activity
AU2002251944A1 (en) * 2001-02-15 2002-09-04 Medtronic Minimed, Inc. Polymers functionalized with fluorescent boronate motifs
WO2002093129A2 (en) * 2001-05-15 2002-11-21 University Of Medicine & Dentistry Of New Jersey Methods for analyzing interactions between proteins in live and intact cells
JP3829252B2 (ja) * 2001-06-08 2006-10-04 独立行政法人理化学研究所 蛍光蛋白質
US7045361B2 (en) 2001-09-12 2006-05-16 Medtronic Minimed, Inc. Analyte sensing via acridine-based boronate biosensors
WO2003040670A2 (en) * 2001-11-07 2003-05-15 Sensor Technologies Llc Method of identifying energy transfer sensors for analytes
US6972326B2 (en) * 2001-12-03 2005-12-06 Molecular Probes, Inc. Labeling of immobilized proteins using dipyrrometheneboron difluoride dyes
DE60233347D1 (de) * 2001-12-19 2009-09-24 Univ Chicago Schnellreifende fluoreszierende proteine und verfahren zu deren anwendung
US20050181464A1 (en) * 2002-04-04 2005-08-18 Affinium Pharmaceuticals, Inc. Novel purified polypeptides from bacteria
WO2003093296A2 (en) 2002-05-03 2003-11-13 Sequenom, Inc. Kinase anchor protein muteins, peptides thereof, and related methods
US7195884B2 (en) * 2002-07-19 2007-03-27 Promega Corp. Methods and kits for transferases
CA2493993A1 (en) * 2002-08-01 2004-02-12 Sensor Technologies Llc Method of measuring molecular interactions
US7491545B2 (en) * 2002-10-01 2009-02-17 Richard Thompson Excitation ratiometric fluoroscent biosensor for zinc ion at picomolar levels
AU2003285844A1 (en) 2002-11-12 2004-06-03 Zakrytoe Aktsionernoe Obschestvo "Evrogen" Fluorescent proteins and chromoproteins from non-aequorea hydrozoa species and methods for using same
AU2003287115A1 (en) 2002-12-26 2004-07-22 Zakrytoe Aktsionernoe Obschestvo "Evrogen" Fluorescent proteins from copepoda species and methods for using same
US9068234B2 (en) 2003-01-21 2015-06-30 Ptc Therapeutics, Inc. Methods and agents for screening for compounds capable of modulating gene expression
EP1604011A4 (de) * 2003-01-21 2009-12-09 Ptc Therapeutics Inc Verfahren zur identifizierung von verbindungen, die die von nichttranslatierten bereichen abhängige genexpression modulieren, sowie verfahren zur verwendung davon
US8426194B2 (en) 2003-01-21 2013-04-23 Ptc Therapeutics, Inc. Methods and agents for screening for compounds capable of modulating VEGF expression
EP2319318A3 (de) 2003-03-27 2011-08-17 PTC Therapeutics, Inc. Verfahren zur Identifizierung von Verbindungen mit tRNA-Splicing Endonuklease als Aufhänger, und Verwendungen für diese Verbindungen als anti-Proliferationsmittel
WO2004087884A2 (en) 2003-03-27 2004-10-14 Ptc Therapeutics, Inc TARGETING ENZYMES OF THE tRNA SPLICING PATHWAY FOR IDENTIFICATION OF ANTI-FUNGAL AND/OR ANTI-PROLIFERATIVE MOLECULES
WO2004087070A2 (en) * 2003-03-27 2004-10-14 Ptc Therapeutics, Inc. METHODS OF IDENTIFYING COMPOUNDS THAT TARGET tRNA SPLICING ENDONUCLEASE AND USES OF SAID COMPOUNDS AS ANTI-FUNGAL AGENTS
JP4990621B2 (ja) * 2003-07-02 2012-08-01 ピーティーシー セラピューティクス，インコーポレーテッド Ｒｎａプロセシングタンパク質複合体及びその使用
US20050095174A1 (en) * 2003-10-31 2005-05-05 Wolf David E. Semipermeable sensors for detecting analyte
WO2005049868A1 (en) * 2003-11-17 2005-06-02 Pct Therapeutics, Inc. Methods and agents for screening for compounds capable of modulating her2 expression
WO2005100387A1 (en) 2004-04-07 2005-10-27 The University Of Chicago Monomeric red fluorescent proteins
US7465810B2 (en) * 2004-10-25 2008-12-16 Anaspec, Inc. Reactive 1,3′-crosslinked carbocyanine
US7417131B2 (en) * 2005-11-04 2008-08-26 Evrogen Joint Stock Company Modified green fluorescent proteins and methods for using same
EP1994149B1 (de) 2006-01-25 2010-09-29 Evrogen IP Neue fluoreszenzproteine und verfahren zur verwendung davon
US8563703B2 (en) 2006-01-25 2013-10-22 Evrogen IP Joint Stock Company Fluorescent proteins and methods for using same
US8680235B2 (en) * 2006-09-22 2014-03-25 Stowers Institute For Medical Research Branchiostoma derived fluorescent proteins
DE102007017051A1 (de) * 2007-04-11 2008-10-16 Humboldt-Universität Zu Berlin Dual-markierte Fluoreszenzsonden zur Detektion von Proteinen
US8283115B1 (en) 2007-06-20 2012-10-09 Ptc Therapeutics, Inc. Methods of screening for compounds for treating muscular dystrophy using UTRN mRNA translation regulation
US8283116B1 (en) 2007-06-22 2012-10-09 Ptc Therapeutics, Inc. Methods of screening for compounds for treating spinal muscular atrophy using SMN mRNA translation regulation
US8679749B2 (en) * 2007-11-01 2014-03-25 The University Of Chicago Red fluorescent proteins with enhanced bacterial expression, increased brightness and reduced aggregation
WO2011087789A2 (en) 2009-12-22 2011-07-21 Becton, Dickinson And Company Methods for the detection of microorganisms
EP2615111A1 (de) 2012-01-10 2013-07-17 Laboratorios del Dr. Esteve S.A. Verfahren zur Identifizierung von Liganden für Sigma-1-Rezeptoren
CN105062465B (zh) * 2015-07-31 2018-06-15 山东大学 一类环境敏感型的α1-肾上腺素能受体近红外荧光配体及其应用
PT109877A (pt) * 2017-01-26 2018-07-26 Inst Superior Tecnico Método ótico para a medição da concentração de oxigénio em sistemas de combustível.

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4828981A (en) * 1983-08-24 1989-05-09 Synbiotics Corporation Immunoassays for determining Dirofilaria immitis infection using antiidiotype monoclonal antibody reagents
US4822733A (en) * 1985-05-28 1989-04-18 Amoco Corporation Lifetime-resolved assay procedures
US4868103A (en) * 1986-02-19 1989-09-19 Enzo Biochem, Inc. Analyte detection by means of energy transfer

Also Published As

Publication number Publication date
JP3208486B2 (ja) 2001-09-10
EP0537270A1 (de) 1993-04-21
DK0537270T3 (da) 1999-06-07
JPH05508772A (ja) 1993-12-09
EP0537270A4 (en) 1996-07-24
US5439797A (en) 1995-08-08
EP0537270B1 (de) 1998-09-09
WO1992000388A1 (en) 1992-01-09
DE69130171D1 (de) 1998-10-15
DE69130171T2 (de) 1999-06-24

Similar Documents

Publication Publication Date Title
ATE170980T1 (de) Bestimmung von analyten durch übertragung von fluoreszenzenergie
Huber et al. Dual lifetime referencing as applied to a chloride optical sensor
Papkovsky Luminescent porphyrins as probes for optical (bio) sensors
Huber et al. Optical sensor for seawater salinity
Mayer et al. Dissolved protein fluorescence in two Maine estuaries
Kosch et al. Strategies to design pH optodes with luminescence decay times in the microsecond time regime
DE69313611T2 (de) Biospezifisches multiparameter-analyseverfahren
Buschmann et al. Spectroscopic study and evaluation of red-absorbing fluorescent dyes
Gunnlaugsson et al. Fluorescent photoinduced electron transfer (PET) sensors for anions; from design to potential application
Kocincova et al. Fiber-optic microsensors for simultaneous sensing of oxygen and pH, and of oxygen and temperature
US6197258B1 (en) Photoluminescent sensors of chemical analytes
DE50115064D1 (de) Sensor zur lumineszenz-optischen bestimmung eines analyten
Shimozono et al. Concatenation of cyan and yellow fluorescent proteins for efficient resonance energy transfer
Turel et al. Direct UV-LED lifetime pH sensor based on a semi-permeable sol–gel membrane immobilized luminescent Eu3+ chelate complex
ATE63002T1 (de) Testverfahren unter verwendung eines in eine zelle aufnehmbaren fluoreszierenden farbstoffs.
Lei et al. A ratiometric fluorescent probe for sensing hydrogen peroxide based on a hemicyanine–naphthol fluorophore
Huber et al. Nitrate-selective optical sensor applying a lipophilic fluorescent potential-sensitive dye
Fron et al. Revealing the excited-state dynamics of the fluorescent protein Dendra2
Nieva-Gomez et al. Membrane changes in Escherichia coli induced by colicin Ia and agents known to disrupt energy transduction
Grimm et al. Rhodamines with a chloronicotinic acid fragment for live cell superresolution STED microscopy
Benink et al. Direct pH measurements by using subcellular targeting of 5 (and 6-) carboxyseminaphthorhodafluor in mammalian cells
Shimizu et al. Colorimetric detection of ATP by a chlorophosphonazo III-based Mg2+ complex in aqueous solution via indicator displacement approach
US9193990B2 (en) Bioluminescent metal ion assay
GB2238117A (en) Environmental sampling.
Kirillova et al. Effect of halogenated fluorescent compounds on bioluminescent reactions

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties