ATE187178T1 - Gel auf basis eines derivat von glykosaminoglykan; copolymers aus acrylamid und diesem derivat; verfahren zum nachweis von enzymen - Google Patents
Gel auf basis eines derivat von glykosaminoglykan; copolymers aus acrylamid und diesem derivat; verfahren zum nachweis von enzymenInfo
- Publication number
- ATE187178T1 ATE187178T1 AT95111522T AT95111522T ATE187178T1 AT E187178 T1 ATE187178 T1 AT E187178T1 AT 95111522 T AT95111522 T AT 95111522T AT 95111522 T AT95111522 T AT 95111522T AT E187178 T1 ATE187178 T1 AT E187178T1
- Authority
- AT
- Austria
- Prior art keywords
- glycosaminoglycan
- derivative
- gel
- acrylamide
- degrading enzyme
- Prior art date
Links
- 229920002683 Glycosaminoglycan Polymers 0.000 title abstract 7
- 102000004190 Enzymes Human genes 0.000 title abstract 5
- 108090000790 Enzymes Proteins 0.000 title abstract 5
- 229920001577 copolymer Polymers 0.000 title abstract 4
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- 238000001962 electrophoresis Methods 0.000 abstract 2
- 125000003903 2-propenyl group Chemical group [H]C([*])([H])C([H])=C([H])[H] 0.000 abstract 1
- SQDAZGGFXASXDW-UHFFFAOYSA-N 5-bromo-2-(trifluoromethoxy)pyridine Chemical compound FC(F)(F)OC1=CC=C(Br)C=N1 SQDAZGGFXASXDW-UHFFFAOYSA-N 0.000 abstract 1
- 229920002567 Chondroitin Polymers 0.000 abstract 1
- 229920001287 Chondroitin sulfate Polymers 0.000 abstract 1
- 230000015556 catabolic process Effects 0.000 abstract 1
- DLGJWSVWTWEWBJ-HGGSSLSASA-N chondroitin Chemical compound CC(O)=N[C@@H]1[C@H](O)O[C@H](CO)[C@H](O)[C@@H]1OC1[C@H](O)[C@H](O)C=C(C(O)=O)O1 DLGJWSVWTWEWBJ-HGGSSLSASA-N 0.000 abstract 1
- 229940059329 chondroitin sulfate Drugs 0.000 abstract 1
- 150000001875 compounds Chemical class 0.000 abstract 1
- 238000006731 degradation reaction Methods 0.000 abstract 1
- 238000006911 enzymatic reaction Methods 0.000 abstract 1
- 229920002401 polyacrylamide Polymers 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
- C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
- C08B37/0069—Chondroitin-4-sulfate, i.e. chondroitin sulfate A; Dermatan sulfate, i.e. chondroitin sulfate B or beta-heparin; Chondroitin-6-sulfate, i.e. chondroitin sulfate C; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
- C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
- C08B37/0075—Heparin; Heparan sulfate; Derivatives thereof, e.g. heparosan; Purification or extraction methods thereof
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44747—Composition of gel or of carrier mixture
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2400/00—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
- G01N2400/10—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
- G01N2400/38—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence, e.g. gluco- or galactomannans, Konjac gum, Locust bean gum or Guar gum
- G01N2400/40—Glycosaminoglycans, i.e. GAG or mucopolysaccharides, e.g. chondroitin sulfate, dermatan sulfate, hyaluronic acid, heparin, heparan sulfate, and related sulfated polysaccharides
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Polymers & Plastics (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Materials Engineering (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Electrochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Dispersion Chemistry (AREA)
- Genetics & Genomics (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Dermatology (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP19128594 | 1994-07-22 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE187178T1 true ATE187178T1 (de) | 1999-12-15 |
Family
ID=16272027
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT95111522T ATE187178T1 (de) | 1994-07-22 | 1995-07-21 | Gel auf basis eines derivat von glykosaminoglykan; copolymers aus acrylamid und diesem derivat; verfahren zum nachweis von enzymen |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US6228998B1 (de) |
| EP (1) | EP0693499B1 (de) |
| AT (1) | ATE187178T1 (de) |
| DE (1) | DE69513579T2 (de) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6063378A (en) * | 1997-08-22 | 2000-05-16 | Seikagaku Corporation | Therapeutic agent for herniated intervertebral disc |
| DE19818077A1 (de) * | 1998-04-22 | 1999-10-28 | Biotechnolog Forschung Gmbh | Immobilisierte Substrate, damit gefertigte Trenngele und Verfahren zum Nachweis von Enzymaktivitäten nach elektrophoretischer Protein-Trennung |
| JP3493608B2 (ja) * | 2001-11-28 | 2004-02-03 | 学校法人慶應義塾 | 医用高分子及びその用途 |
| JP4828795B2 (ja) * | 2002-03-11 | 2011-11-30 | モメンタ ファーマシューティカルズ インコーポレイテッド | 硫酸化多糖類の分析 |
| US8673333B2 (en) | 2002-09-25 | 2014-03-18 | The Johns Hopkins University | Cross-linked polymer matrices, and methods of making and using same |
| US9139876B1 (en) | 2007-05-03 | 2015-09-22 | Momenta Pharmacueticals, Inc. | Method of analyzing a preparation of a low molecular weight heparin |
| GB0720030D0 (en) * | 2007-10-12 | 2007-11-21 | Cambridge Entpr Ltd | Depolymerase assay |
| WO2011090948A1 (en) * | 2010-01-19 | 2011-07-28 | Momenta Pharmaceuticals, Inc. | Evaluating heparin preparations |
| WO2012115952A1 (en) | 2011-02-21 | 2012-08-30 | Momenta Pharmaceuticals, Inc. | Evaluating heparin preparations |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1206666A (en) | 1982-02-19 | 1986-06-24 | Samuel Nochumson | Copolymer of acrylamide and polysaccharide resin as electrophoretic gel medium |
| US4859581A (en) * | 1986-03-10 | 1989-08-22 | Board Of Regents, The University Of Texas System | Endoglycosidase assay |
| JP2726275B2 (ja) * | 1988-08-24 | 1998-03-11 | 生化学工業株式会社 | グリコサミノグリカン分解酵素の精製法 |
| JP2975632B2 (ja) | 1990-03-30 | 1999-11-10 | 生化学工業株式会社 | グリコサミノグリカン修飾プロテイン |
| WO1992001720A1 (fr) | 1990-07-24 | 1992-02-06 | Seikagaku Kogyo Kabushiki Kaisha | Glycosaminoglycane se combinant a un phospholipide ou a un lipide, production de ce compose et inhibiteur de metastase du cancer |
-
1995
- 1995-07-20 US US08/504,675 patent/US6228998B1/en not_active Expired - Lifetime
- 1995-07-21 DE DE69513579T patent/DE69513579T2/de not_active Expired - Fee Related
- 1995-07-21 EP EP95111522A patent/EP0693499B1/de not_active Expired - Lifetime
- 1995-07-21 AT AT95111522T patent/ATE187178T1/de not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| DE69513579T2 (de) | 2000-04-27 |
| US6228998B1 (en) | 2001-05-08 |
| EP0693499B1 (de) | 1999-12-01 |
| EP0693499A1 (de) | 1996-01-24 |
| DE69513579D1 (de) | 2000-01-05 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |