ATE191934T1 - Verfahren zur isothermer amplifikation von nukleinsäure molekülen - Google Patents
Verfahren zur isothermer amplifikation von nukleinsäure molekülenInfo
- Publication number
- ATE191934T1 ATE191934T1 AT96906256T AT96906256T ATE191934T1 AT E191934 T1 ATE191934 T1 AT E191934T1 AT 96906256 T AT96906256 T AT 96906256T AT 96906256 T AT96906256 T AT 96906256T AT E191934 T1 ATE191934 T1 AT E191934T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acid
- acid molecules
- isothermal amplification
- amplification
- amplifying
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6862—Ligase chain reaction [LCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Steroid Compounds (AREA)
- Crystals, And After-Treatments Of Crystals (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/383,327 US5591609A (en) | 1992-08-04 | 1995-02-03 | Methods for the isothermal amplification of nucleic acid molecules |
| US08/533,852 US5614389A (en) | 1992-08-04 | 1995-09-26 | Methods for the isothermal amplification of nucleic acid molecules |
| PCT/US1996/001379 WO1996023904A1 (en) | 1995-02-03 | 1996-02-01 | Methods for the isothermal amplification of nucleic acid molecules |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE191934T1 true ATE191934T1 (de) | 2000-05-15 |
Family
ID=27010138
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT96906256T ATE191934T1 (de) | 1995-02-03 | 1996-02-01 | Verfahren zur isothermer amplifikation von nukleinsäure molekülen |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US5614389A (de) |
| EP (1) | EP0807186B1 (de) |
| JP (2) | JPH10513058A (de) |
| AT (1) | ATE191934T1 (de) |
| AU (1) | AU4969996A (de) |
| CA (1) | CA2212185C (de) |
| DE (1) | DE69607830T2 (de) |
| WO (1) | WO1996023904A1 (de) |
Families Citing this family (80)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5733733A (en) * | 1992-08-04 | 1998-03-31 | Replicon, Inc. | Methods for the isothermal amplification of nucleic acid molecules |
| US6261808B1 (en) | 1992-08-04 | 2001-07-17 | Replicon, Inc. | Amplification of nucleic acid molecules via circular replicons |
| US5834202A (en) | 1992-08-04 | 1998-11-10 | Replicon, Inc. | Methods for the isothermal amplification of nucleic acid molecules |
| WO1994003624A1 (en) * | 1992-08-04 | 1994-02-17 | Auerbach Jeffrey I | Methods for the isothermal amplification of nucleic acid molecules |
| NZ500843A (en) | 1995-06-07 | 2002-03-28 | Invitrogen Corp | A method of DNA recombination which is selectable to select for cells containing a product and against cells only harbouring the insert donor |
| US6143557A (en) * | 1995-06-07 | 2000-11-07 | Life Technologies, Inc. | Recombination cloning using engineered recombination sites |
| US6720140B1 (en) | 1995-06-07 | 2004-04-13 | Invitrogen Corporation | Recombinational cloning using engineered recombination sites |
| US6964861B1 (en) * | 1998-11-13 | 2005-11-15 | Invitrogen Corporation | Enhanced in vitro recombinational cloning of using ribosomal proteins |
| US6143495A (en) | 1995-11-21 | 2000-11-07 | Yale University | Unimolecular segment amplification and sequencing |
| US5854033A (en) | 1995-11-21 | 1998-12-29 | Yale University | Rolling circle replication reporter systems |
| AU746549B2 (en) | 1996-11-20 | 2002-05-02 | Becton Dickinson & Company | Microfabricated isothermal nucleic acid amplification devices and methods |
| US6482590B1 (en) | 1996-12-20 | 2002-11-19 | Aventis Behring Gmbh | Method for polynucleotide amplification |
| US5851808A (en) | 1997-02-28 | 1998-12-22 | Baylor College Of Medicine | Rapid subcloning using site-specific recombination |
| CN101125873A (zh) * | 1997-10-24 | 2008-02-20 | 茵维特罗根公司 | 利用具重组位点的核酸进行重组克隆 |
| US7351578B2 (en) * | 1999-12-10 | 2008-04-01 | Invitrogen Corp. | Use of multiple recombination sites with unique specificity in recombinational cloning |
| JP4303418B2 (ja) * | 1997-10-24 | 2009-07-29 | ライフ テクノロジーズ コーポレーション | 組換え部位を有する核酸を使用する組換えクローニング |
| US6054274A (en) * | 1997-11-12 | 2000-04-25 | Hewlett-Packard Company | Method of amplifying the signal of target nucleic acid sequence analyte |
| EP1098996A1 (de) | 1998-07-20 | 2001-05-16 | Yale University | Verfahren zum nachweis von nukleinsäuren unter verwendung zielgerichteter ligation von zweiteiligen primern |
| US6991922B2 (en) * | 1998-08-12 | 2006-01-31 | Proteus S.A. | Process for in vitro creation of recombinant polynucleotide sequences by oriented ligation |
| US6951719B1 (en) * | 1999-08-11 | 2005-10-04 | Proteus S.A. | Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained |
| WO2000015849A1 (en) | 1998-09-15 | 2000-03-23 | Yale University | Artificial long terminal repeat vectors |
| CA2342838A1 (en) * | 1998-09-15 | 2000-03-23 | Yale University | Molecular cloning using rolling circle amplification |
| US7135284B1 (en) * | 1999-02-05 | 2006-11-14 | Integrated Dna Technologies, Inc. | Primer extension methods for production of high specific activity nucleic acid probes |
| EP2119788A1 (de) * | 1999-03-02 | 2009-11-18 | Life Technologies Corporation | Zusammensetzungen und Verfahren zur Verwendung beim Rekombinationsklonen von Nukleinsäuren |
| US6890726B1 (en) | 1999-04-06 | 2005-05-10 | Oklahoma Medical Research Foundation | Method for selecting recombinase variants with altered specificity |
| JP2003527087A (ja) | 1999-08-13 | 2003-09-16 | イェール・ユニバーシティ | バイナリーコード化配列タグ |
| CN1757724B (zh) | 1999-12-10 | 2014-06-11 | 茵维特罗根公司 | 具有独特特异性的多个重组位点在重组克隆中的用途 |
| US7244560B2 (en) * | 2000-05-21 | 2007-07-17 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
| US20060166227A1 (en) * | 2000-06-20 | 2006-07-27 | Stephen Kingsmore | Protein expression profiling |
| US6323009B1 (en) * | 2000-06-28 | 2001-11-27 | Molecular Staging, Inc. | Multiply-primed amplification of nucleic acid sequences |
| US6686157B2 (en) | 2000-06-30 | 2004-02-03 | Molecular Staging Inc. | Signal amplification with lollipop probes |
| US7198924B2 (en) * | 2000-12-11 | 2007-04-03 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
| US6573051B2 (en) | 2001-03-09 | 2003-06-03 | Molecular Staging, Inc. | Open circle probes with intramolecular stem structures |
| CA2444195A1 (en) * | 2001-04-19 | 2002-10-31 | Invitrogen Corporation | Compositions and methods for recombinational cloning of nucleic acid molecules |
| JP2004532636A (ja) * | 2001-05-21 | 2004-10-28 | インヴィトロジェン コーポレーション | 核酸分子の単離に用いるための組成物および方法 |
| US8137911B2 (en) | 2001-05-22 | 2012-03-20 | Cellscript, Inc. | Preparation and use of single-stranded transcription substrates for synthesis of transcription products corresponding to target sequences |
| RU2180311C1 (ru) * | 2001-07-05 | 2002-03-10 | Мешалкин Георгий Алексеевич | Защитная пробка |
| AU2002365212A1 (en) * | 2001-07-15 | 2003-09-02 | Keck Graduate Institute | Gene expression analysis using nicking agents |
| US7553619B2 (en) * | 2002-02-08 | 2009-06-30 | Qiagen Gmbh | Detection method using dissociated rolling circle amplification |
| US7399590B2 (en) | 2002-02-21 | 2008-07-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| US8030000B2 (en) | 2002-02-21 | 2011-10-04 | Alere San Diego, Inc. | Recombinase polymerase amplification |
| CA2476481C (en) | 2002-02-21 | 2016-01-26 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| US7211382B2 (en) * | 2002-04-09 | 2007-05-01 | Orchid Cellmark Inc. | Primer extension using modified nucleotides |
| US20040219516A1 (en) * | 2002-07-18 | 2004-11-04 | Invitrogen Corporation | Viral vectors containing recombination sites |
| US7413857B2 (en) * | 2002-11-21 | 2008-08-19 | Epicentre Technologies | Methods for using riboprimers for strand displacement replication of target sequences |
| US20040121338A1 (en) * | 2002-12-19 | 2004-06-24 | Alsmadi Osama A. | Real-time detection of rolling circle amplification products |
| US9487823B2 (en) * | 2002-12-20 | 2016-11-08 | Qiagen Gmbh | Nucleic acid amplification |
| US6977153B2 (en) | 2002-12-31 | 2005-12-20 | Qiagen Gmbh | Rolling circle amplification of RNA |
| US8043834B2 (en) | 2003-03-31 | 2011-10-25 | Qiagen Gmbh | Universal reagents for rolling circle amplification and methods of use |
| US20050095615A1 (en) * | 2003-06-26 | 2005-05-05 | Welch Peter J. | Methods and compositions for detecting promoter activity and expressing fusion proteins |
| GB0318110D0 (en) * | 2003-08-01 | 2003-09-03 | Isaeo Ltd | Methods and kits for detecting an enzyme capable of modifying a nucleic acid |
| US8304189B2 (en) * | 2003-12-01 | 2012-11-06 | Life Technologies Corporation | Nucleic acid molecules containing recombination sites and methods of using the same |
| US20100022402A1 (en) * | 2004-11-23 | 2010-01-28 | Sancar Adhya | Methods and Compositions for the In Vitro High-Throughput Detection of Protein/Protein Interactions |
| US8309303B2 (en) * | 2005-04-01 | 2012-11-13 | Qiagen Gmbh | Reverse transcription and amplification of RNA with simultaneous degradation of DNA |
| WO2006108423A2 (en) * | 2005-04-12 | 2006-10-19 | In Situ Rcp A/S Under Founding | Methods for production of oligonucleotides |
| US7989166B2 (en) * | 2005-04-12 | 2011-08-02 | In Situ Rcp A/S | Circle probes and their use in the identification of biomolecules |
| EP1929049B1 (de) | 2005-07-25 | 2013-04-10 | Alere San Diego, Inc. | Verfahren zur multiplexierung einer rekombinase-polymerase-verstärkung |
| EP1762627A1 (de) | 2005-09-09 | 2007-03-14 | Qiagen GmbH | Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion |
| JP2009511019A (ja) * | 2005-10-06 | 2009-03-19 | ルシジェン コーポレイション | 耐熱性ウィルス性ポリメラーゼ及びその使用法 |
| JP2009535053A (ja) | 2006-05-04 | 2009-10-01 | エーエスエム サイエンティフィック， インコーポレイテッド | レコンビナーゼポリメラーゼ増幅 |
| DE102006020885A1 (de) * | 2006-05-05 | 2007-11-08 | Qiagen Gmbh | Einführung von Sequenzelementen in Nukleinsäuren |
| US7501254B2 (en) | 2006-07-20 | 2009-03-10 | Ghc Technologies, Inc. | Methods and compositions for amplification and capture of nucleic acid sequences |
| EP2079850B1 (de) * | 2006-09-20 | 2016-02-24 | Colorado State University Research Foundation | Erkennung chromosomaler inversionen |
| US9689031B2 (en) | 2007-07-14 | 2017-06-27 | Ionian Technologies, Inc. | Nicking and extension amplification reaction for the exponential amplification of nucleic acids |
| ES2743029T3 (es) | 2008-10-24 | 2020-02-18 | Epicentre Tech Corporation | Composiciones de extremo del transposón y métodos para modificar ácidos nucleicos |
| US9080211B2 (en) | 2008-10-24 | 2015-07-14 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
| US9469867B2 (en) | 2009-05-20 | 2016-10-18 | Alere San Diego, Inc. | DNA glycosylase/lyase and AP endonuclease substrates |
| EP3360974B1 (de) | 2009-06-05 | 2025-04-09 | Abbott Diagnostics Scarborough, Inc. | Reagentien zur rekombinase polymerase amplifikation |
| EP2369325A1 (de) | 2010-03-12 | 2011-09-28 | Eppendorf Ag | Arrayanalyse für Online-Detektion |
| EP2753715A4 (de) | 2011-09-09 | 2015-05-20 | Univ Leland Stanford Junior | Verfahren zur gewinnung einer sequenz |
| US9352312B2 (en) | 2011-09-23 | 2016-05-31 | Alere Switzerland Gmbh | System and apparatus for reactions |
| CN114214394A (zh) | 2012-06-08 | 2022-03-22 | 爱奥尼安技术公司 | 核苷酸扩增反应 |
| US10017759B2 (en) | 2014-06-26 | 2018-07-10 | Illumina, Inc. | Library preparation of tagged nucleic acid |
| CN107109495B (zh) | 2014-11-11 | 2021-12-24 | 伊鲁米那股份有限公司 | 使用crispr-cas系统的多核苷酸扩增 |
| EP3455372B1 (de) | 2016-05-11 | 2019-10-30 | Illumina, Inc. | Polynukleotidanreicherung und -amplifikation unter verwendung von argonautensystemen |
| EP3565907B1 (de) | 2017-01-06 | 2022-05-04 | Editas Medicine, Inc. | Verfahren zur beurteilung der nukleasespaltung |
| US10914729B2 (en) | 2017-05-22 | 2021-02-09 | The Trustees Of Princeton University | Methods for detecting protein binding sequences and tagging nucleic acids |
| BR112023018157A2 (pt) | 2021-03-09 | 2023-10-31 | Illumina Inc | Análise de expressão de variantes codificantes de proteína em células |
| KR20230154078A (ko) | 2021-03-09 | 2023-11-07 | 일루미나, 인코포레이티드 | CAS-gRNA 리보핵단백질을 사용한 게놈 라이브러리 제작 및 표적화된 후성적 검정 |
| ES3035187T3 (en) | 2021-08-11 | 2025-08-29 | Illumina Inc | Detection of analytes using targeted epigenetic assays, proximity-induced tagmentation, strand invasion, restriction, or ligation |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4582788A (en) * | 1982-01-22 | 1986-04-15 | Cetus Corporation | HLA typing method and cDNA probes used therein |
| US4673640A (en) * | 1984-04-30 | 1987-06-16 | Biotechnica International, Inc. | Regulated protein production using site-specific recombination |
| US4683194A (en) * | 1984-05-29 | 1987-07-28 | Cetus Corporation | Method for detection of polymorphic restriction sites and nucleic acid sequences |
| CA1293460C (en) * | 1985-10-07 | 1991-12-24 | Brian Lee Sauer | Site-specific recombination of dna in yeast |
| IL86724A (en) * | 1987-06-19 | 1995-01-24 | Siska Diagnostics Inc | Methods and kits for amplification and testing of nucleic acid sequences |
| JP2846018B2 (ja) * | 1988-01-21 | 1999-01-13 | ジェネンテク，インコーポレイテッド | 核酸配列の増幅および検出 |
| CA1340807C (en) * | 1988-02-24 | 1999-11-02 | Lawrence T. Malek | Nucleic acid amplification process |
| CA2012983A1 (en) * | 1989-03-27 | 1990-09-27 | Sydney Brenner | Process for nucleic acid detection by binary amplification |
| WO1994003624A1 (en) * | 1992-08-04 | 1994-02-17 | Auerbach Jeffrey I | Methods for the isothermal amplification of nucleic acid molecules |
| US6683202B2 (en) * | 2001-02-22 | 2004-01-27 | Tokyo Ohka, Kogyo Co., Ltd. | Fluorine-containing monomeric ester compound for base resin in photoresist composition |
-
1995
- 1995-09-26 US US08/533,852 patent/US5614389A/en not_active Expired - Lifetime
-
1996
- 1996-02-01 CA CA002212185A patent/CA2212185C/en not_active Expired - Fee Related
- 1996-02-01 AT AT96906256T patent/ATE191934T1/de not_active IP Right Cessation
- 1996-02-01 EP EP96906256A patent/EP0807186B1/de not_active Expired - Lifetime
- 1996-02-01 DE DE69607830T patent/DE69607830T2/de not_active Expired - Lifetime
- 1996-02-01 WO PCT/US1996/001379 patent/WO1996023904A1/en not_active Ceased
- 1996-02-01 JP JP8523724A patent/JPH10513058A/ja not_active Withdrawn
- 1996-02-01 AU AU49699/96A patent/AU4969996A/en not_active Abandoned
-
2007
- 2007-03-09 JP JP2007061113A patent/JP2007190028A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| US5614389A (en) | 1997-03-25 |
| CA2212185C (en) | 2008-01-22 |
| WO1996023904A1 (en) | 1996-08-08 |
| DE69607830D1 (de) | 2000-05-25 |
| AU4969996A (en) | 1996-08-21 |
| CA2212185A1 (en) | 1996-08-08 |
| EP0807186B1 (de) | 2000-04-19 |
| JP2007190028A (ja) | 2007-08-02 |
| DE69607830T2 (de) | 2000-11-30 |
| JPH10513058A (ja) | 1998-12-15 |
| EP0807186A1 (de) | 1997-11-19 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |