ATE191934T1 - Verfahren zur isothermer amplifikation von nukleinsäure molekülen - Google Patents
Verfahren zur isothermer amplifikation von nukleinsäure molekülenInfo
- Publication number
- ATE191934T1 ATE191934T1 AT96906256T AT96906256T ATE191934T1 AT E191934 T1 ATE191934 T1 AT E191934T1 AT 96906256 T AT96906256 T AT 96906256T AT 96906256 T AT96906256 T AT 96906256T AT E191934 T1 ATE191934 T1 AT E191934T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acid
- acid molecules
- isothermal amplification
- amplification
- amplifying
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6862—Ligase chain reaction [LCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Crystals, And After-Treatments Of Crystals (AREA)
- Steroid Compounds (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/383,327 US5591609A (en) | 1992-08-04 | 1995-02-03 | Methods for the isothermal amplification of nucleic acid molecules |
| US08/533,852 US5614389A (en) | 1992-08-04 | 1995-09-26 | Methods for the isothermal amplification of nucleic acid molecules |
| PCT/US1996/001379 WO1996023904A1 (en) | 1995-02-03 | 1996-02-01 | Methods for the isothermal amplification of nucleic acid molecules |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE191934T1 true ATE191934T1 (de) | 2000-05-15 |
Family
ID=27010138
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT96906256T ATE191934T1 (de) | 1995-02-03 | 1996-02-01 | Verfahren zur isothermer amplifikation von nukleinsäure molekülen |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US5614389A (de) |
| EP (1) | EP0807186B1 (de) |
| JP (2) | JPH10513058A (de) |
| AT (1) | ATE191934T1 (de) |
| AU (1) | AU4969996A (de) |
| CA (1) | CA2212185C (de) |
| DE (1) | DE69607830T2 (de) |
| WO (1) | WO1996023904A1 (de) |
Families Citing this family (82)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1994003624A1 (en) * | 1992-08-04 | 1994-02-17 | Auerbach Jeffrey I | Methods for the isothermal amplification of nucleic acid molecules |
| US6261808B1 (en) | 1992-08-04 | 2001-07-17 | Replicon, Inc. | Amplification of nucleic acid molecules via circular replicons |
| US5834202A (en) | 1992-08-04 | 1998-11-10 | Replicon, Inc. | Methods for the isothermal amplification of nucleic acid molecules |
| US5733733A (en) * | 1992-08-04 | 1998-03-31 | Replicon, Inc. | Methods for the isothermal amplification of nucleic acid molecules |
| US6143557A (en) * | 1995-06-07 | 2000-11-07 | Life Technologies, Inc. | Recombination cloning using engineered recombination sites |
| US6720140B1 (en) | 1995-06-07 | 2004-04-13 | Invitrogen Corporation | Recombinational cloning using engineered recombination sites |
| EP0937098B1 (de) | 1995-06-07 | 2002-08-14 | Invitrogen Corporation | Rekombinatorische klonierung in vitro unter verwendung genmanipulierter rekombinationsorte |
| US6964861B1 (en) * | 1998-11-13 | 2005-11-15 | Invitrogen Corporation | Enhanced in vitro recombinational cloning of using ribosomal proteins |
| US5854033A (en) | 1995-11-21 | 1998-12-29 | Yale University | Rolling circle replication reporter systems |
| DK0862656T3 (da) | 1995-11-21 | 2001-04-09 | Univ Yale | Unimolekylær segmentamplifikation og -detektering |
| CA2276251A1 (en) | 1996-11-20 | 1998-05-28 | The Regents Of The University Of Michigan | Microfabricated isothermal nucleic acid amplification devices and methods |
| US6482590B1 (en) | 1996-12-20 | 2002-11-19 | Aventis Behring Gmbh | Method for polynucleotide amplification |
| US5851808A (en) * | 1997-02-28 | 1998-12-22 | Baylor College Of Medicine | Rapid subcloning using site-specific recombination |
| CN101125873A (zh) * | 1997-10-24 | 2008-02-20 | 茵维特罗根公司 | 利用具重组位点的核酸进行重组克隆 |
| US7351578B2 (en) * | 1999-12-10 | 2008-04-01 | Invitrogen Corp. | Use of multiple recombination sites with unique specificity in recombinational cloning |
| DE69836092T2 (de) | 1997-10-24 | 2007-05-10 | Invitrogen Corp., Carlsbad | Rekombinatorisches klonieren unter verwendung von nukleinsaüren mit rekombinationsstellen |
| US6054274A (en) * | 1997-11-12 | 2000-04-25 | Hewlett-Packard Company | Method of amplifying the signal of target nucleic acid sequence analyte |
| WO2000004193A1 (en) | 1998-07-20 | 2000-01-27 | Yale University | Method for detecting nucleic acids using target-mediated ligation of bipartite primers |
| US6951719B1 (en) * | 1999-08-11 | 2005-10-04 | Proteus S.A. | Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained |
| US6991922B2 (en) * | 1998-08-12 | 2006-01-31 | Proteus S.A. | Process for in vitro creation of recombinant polynucleotide sequences by oriented ligation |
| WO2000015779A2 (en) | 1998-09-15 | 2000-03-23 | Yale University | Molecular cloning using rolling circle amplification |
| JP2002525078A (ja) * | 1998-09-15 | 2002-08-13 | イェール ユニバーシティ | 人工的長末端反復配列 |
| US7135284B1 (en) * | 1999-02-05 | 2006-11-14 | Integrated Dna Technologies, Inc. | Primer extension methods for production of high specific activity nucleic acid probes |
| DE60042969D1 (de) * | 1999-03-02 | 2009-10-29 | Life Technologies Corp | Zubereitungen und methoden zur verwendung in rekombinatorischem klonen von nukleinsäuren |
| US6890726B1 (en) | 1999-04-06 | 2005-05-10 | Oklahoma Medical Research Foundation | Method for selecting recombinase variants with altered specificity |
| ATE318932T1 (de) | 1999-08-13 | 2006-03-15 | Univ Yale | Binär kodierte sequenzmarker |
| ATE391789T1 (de) | 1999-12-10 | 2008-04-15 | Invitrogen Corp | Verwendung einer vielzahl von rekombinationsstellen mit einzigartiger spezifität beim rekombinatorischen klonen |
| US7244560B2 (en) * | 2000-05-21 | 2007-07-17 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
| US20060166227A1 (en) * | 2000-06-20 | 2006-07-27 | Stephen Kingsmore | Protein expression profiling |
| US6323009B1 (en) * | 2000-06-28 | 2001-11-27 | Molecular Staging, Inc. | Multiply-primed amplification of nucleic acid sequences |
| US6686157B2 (en) | 2000-06-30 | 2004-02-03 | Molecular Staging Inc. | Signal amplification with lollipop probes |
| US7198924B2 (en) * | 2000-12-11 | 2007-04-03 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
| US6573051B2 (en) * | 2001-03-09 | 2003-06-03 | Molecular Staging, Inc. | Open circle probes with intramolecular stem structures |
| US20030077804A1 (en) * | 2001-04-19 | 2003-04-24 | Invitrogen Corporation | Compositions and methods for recombinational cloning of nucleic acid molecules |
| CA2448505A1 (en) * | 2001-05-21 | 2002-11-28 | Invitrogen Corporation | Compositions and methods for use in isolation of nucleic acid molecules |
| US8137911B2 (en) | 2001-05-22 | 2012-03-20 | Cellscript, Inc. | Preparation and use of single-stranded transcription substrates for synthesis of transcription products corresponding to target sequences |
| RU2180311C1 (ru) * | 2001-07-05 | 2002-03-10 | Мешалкин Георгий Алексеевич | Защитная пробка |
| EP1470250A2 (de) * | 2001-07-15 | 2004-10-27 | Keck Graduate Institute | Genexpressionsanalyse mit nicking-substanzen |
| US7553619B2 (en) * | 2002-02-08 | 2009-06-30 | Qiagen Gmbh | Detection method using dissociated rolling circle amplification |
| DK1499738T3 (da) | 2002-02-21 | 2008-11-17 | Asm Scient Inc | Recombinase-polymerease-amplifikation |
| US7399590B2 (en) | 2002-02-21 | 2008-07-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| US8030000B2 (en) | 2002-02-21 | 2011-10-04 | Alere San Diego, Inc. | Recombinase polymerase amplification |
| US7211382B2 (en) * | 2002-04-09 | 2007-05-01 | Orchid Cellmark Inc. | Primer extension using modified nucleotides |
| US20040219516A1 (en) * | 2002-07-18 | 2004-11-04 | Invitrogen Corporation | Viral vectors containing recombination sites |
| AU2003297557B2 (en) | 2002-11-21 | 2009-02-26 | Cellscript, Inc. | Methods for using primers that encode one strand of a double-stranded promoter |
| US20040121338A1 (en) * | 2002-12-19 | 2004-06-24 | Alsmadi Osama A. | Real-time detection of rolling circle amplification products |
| US9487823B2 (en) * | 2002-12-20 | 2016-11-08 | Qiagen Gmbh | Nucleic acid amplification |
| US6977153B2 (en) | 2002-12-31 | 2005-12-20 | Qiagen Gmbh | Rolling circle amplification of RNA |
| US8043834B2 (en) | 2003-03-31 | 2011-10-25 | Qiagen Gmbh | Universal reagents for rolling circle amplification and methods of use |
| WO2005028615A2 (en) * | 2003-06-26 | 2005-03-31 | Invitrogen Corporation | Methods and compositions for detecting promoter activity and expressing fusion proteins |
| GB0318110D0 (en) * | 2003-08-01 | 2003-09-03 | Isaeo Ltd | Methods and kits for detecting an enzyme capable of modifying a nucleic acid |
| DE602004027538D1 (de) * | 2003-12-01 | 2010-07-15 | Life Technologies Corp | Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon |
| EP1817591A2 (de) * | 2004-11-23 | 2007-08-15 | The Government of the United States of America, as represented by the Secretary of Health and Human Services | Verfahren und zusammensetzungen zur ex-vivo-hochdurchsatzdetektion von protein-protein-wechselwirkungen |
| EP1863908B1 (de) * | 2005-04-01 | 2010-11-17 | Qiagen GmbH | Reverse transkription und amplifikation von rna bei simultaner degradierung von dna |
| US7989166B2 (en) * | 2005-04-12 | 2011-08-02 | In Situ Rcp A/S | Circle probes and their use in the identification of biomolecules |
| DE602006017192D1 (de) * | 2005-04-12 | 2010-11-11 | Olink Ab | Verfahren zur herstellung von oligonukleotiden |
| EP2829615B1 (de) | 2005-07-25 | 2018-05-09 | Alere San Diego, Inc. | Kit zur Multiplexierung einer Rekombinase-Polymerase-Verstärkung |
| EP1762627A1 (de) | 2005-09-09 | 2007-03-14 | Qiagen GmbH | Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion |
| EP1934339B1 (de) * | 2005-10-06 | 2011-12-14 | Lucigen Corporation | Temperaturstabile virale polymerasen und verwendungsverfahren |
| EP2029782B1 (de) | 2006-05-04 | 2014-11-26 | Alere San Diego, Inc. | Rekombinase-polymerase-verstärkung |
| DE102006020885A1 (de) * | 2006-05-05 | 2007-11-08 | Qiagen Gmbh | Einführung von Sequenzelementen in Nukleinsäuren |
| US7501254B2 (en) | 2006-07-20 | 2009-03-10 | Ghc Technologies, Inc. | Methods and compositions for amplification and capture of nucleic acid sequences |
| EP2079850B1 (de) * | 2006-09-20 | 2016-02-24 | Colorado State University Research Foundation | Erkennung chromosomaler inversionen |
| US9689031B2 (en) | 2007-07-14 | 2017-06-27 | Ionian Technologies, Inc. | Nicking and extension amplification reaction for the exponential amplification of nucleic acids |
| EP2664678B1 (de) | 2008-10-24 | 2014-10-08 | Epicentre Technologies Corporation | Transposonendenzusammensetzungen und Verfahren zur Modifizierung von Nukleinsäuren |
| US9080211B2 (en) * | 2008-10-24 | 2015-07-14 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
| JP5847076B2 (ja) * | 2009-05-20 | 2016-01-20 | バイオサイト インコーポレイテッド | Dnaグリコシラーゼ/リアーゼおよびapエンドヌクレアーゼ基質 |
| WO2010141940A1 (en) | 2009-06-05 | 2010-12-09 | Alere San Diego, Inc. | Recombinase polymerase amplification reagents and kits |
| EP2369325A1 (de) | 2010-03-12 | 2011-09-28 | Eppendorf Ag | Arrayanalyse für Online-Detektion |
| HK1199745A1 (en) | 2011-09-09 | 2015-07-17 | The Board Of Trustees Of The Leland Stanford Junior University | Methods for obtaining a sequence |
| US9352312B2 (en) | 2011-09-23 | 2016-05-31 | Alere Switzerland Gmbh | System and apparatus for reactions |
| CA3096342C (en) | 2012-06-08 | 2023-04-04 | Ionian Technologies, Llc | Nucleic acid amplifications |
| US10017759B2 (en) | 2014-06-26 | 2018-07-10 | Illumina, Inc. | Library preparation of tagged nucleic acid |
| SG11201703779VA (en) | 2014-11-11 | 2017-06-29 | Illumina Inc | Polynucleotide amplification using crispr-cas systems |
| EP3656873A3 (de) | 2016-05-11 | 2020-07-29 | Illumina, Inc. | Polynukleotidanreicherung und -amplifikation unter verwendung von argonautensystemen |
| WO2018129368A2 (en) | 2017-01-06 | 2018-07-12 | Editas Medicine, Inc. | Methods of assessing nuclease cleavage |
| US10914729B2 (en) | 2017-05-22 | 2021-02-09 | The Trustees Of Princeton University | Methods for detecting protein binding sequences and tagging nucleic acids |
| US20260218294A1 (en) * | 2020-11-20 | 2026-07-30 | Axbio Inc. | Methods, systems, and compositions for nucleic acid sequencing |
| KR102809622B1 (ko) | 2021-03-09 | 2025-05-19 | 일루미나, 인코포레이티드 | 세포에서 단백질-코딩 변이체의 발현 분석 |
| KR20230154078A (ko) | 2021-03-09 | 2023-11-07 | 일루미나, 인코포레이티드 | CAS-gRNA 리보핵단백질을 사용한 게놈 라이브러리 제작 및 표적화된 후성적 검정 |
| EP4614146A3 (de) | 2021-08-11 | 2025-11-12 | Illumina, Inc. | Nachweis von analyten unter verwendung gezielter epigenetischer tests, proximitätsinduzierter tagmentation, stranginvasion, -einschränkung oder ligation |
| US20260000702A1 (en) | 2024-06-26 | 2026-01-01 | Flagship Pioneering Innovations Vii, Llc | Therapeutic circular dna forms |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4582788A (en) * | 1982-01-22 | 1986-04-15 | Cetus Corporation | HLA typing method and cDNA probes used therein |
| US4673640A (en) * | 1984-04-30 | 1987-06-16 | Biotechnica International, Inc. | Regulated protein production using site-specific recombination |
| US4683194A (en) * | 1984-05-29 | 1987-07-28 | Cetus Corporation | Method for detection of polymorphic restriction sites and nucleic acid sequences |
| CA1293460C (en) * | 1985-10-07 | 1991-12-24 | Brian Lee Sauer | Site-specific recombination of dna in yeast |
| IL86724A (en) * | 1987-06-19 | 1995-01-24 | Siska Diagnostics Inc | Methods and kits for amplification and testing of nucleic acid sequences |
| EP0359789B1 (de) * | 1988-01-21 | 1993-08-04 | Genentech, Inc. | Verstärkung und nachweis von nukleinsäuresequenzen |
| CA1340807C (en) * | 1988-02-24 | 1999-11-02 | Lawrence T. Malek | Nucleic acid amplification process |
| CA2012983A1 (en) * | 1989-03-27 | 1990-09-27 | Sydney Brenner | Process for nucleic acid detection by binary amplification |
| WO1994003624A1 (en) * | 1992-08-04 | 1994-02-17 | Auerbach Jeffrey I | Methods for the isothermal amplification of nucleic acid molecules |
| US6683202B2 (en) * | 2001-02-22 | 2004-01-27 | Tokyo Ohka, Kogyo Co., Ltd. | Fluorine-containing monomeric ester compound for base resin in photoresist composition |
-
1995
- 1995-09-26 US US08/533,852 patent/US5614389A/en not_active Expired - Lifetime
-
1996
- 1996-02-01 EP EP96906256A patent/EP0807186B1/de not_active Expired - Lifetime
- 1996-02-01 WO PCT/US1996/001379 patent/WO1996023904A1/en not_active Ceased
- 1996-02-01 DE DE69607830T patent/DE69607830T2/de not_active Expired - Lifetime
- 1996-02-01 CA CA002212185A patent/CA2212185C/en not_active Expired - Fee Related
- 1996-02-01 JP JP8523724A patent/JPH10513058A/ja not_active Withdrawn
- 1996-02-01 AU AU49699/96A patent/AU4969996A/en not_active Abandoned
- 1996-02-01 AT AT96906256T patent/ATE191934T1/de not_active IP Right Cessation
-
2007
- 2007-03-09 JP JP2007061113A patent/JP2007190028A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| CA2212185C (en) | 2008-01-22 |
| CA2212185A1 (en) | 1996-08-08 |
| EP0807186B1 (de) | 2000-04-19 |
| EP0807186A1 (de) | 1997-11-19 |
| WO1996023904A1 (en) | 1996-08-08 |
| US5614389A (en) | 1997-03-25 |
| DE69607830D1 (de) | 2000-05-25 |
| DE69607830T2 (de) | 2000-11-30 |
| JPH10513058A (ja) | 1998-12-15 |
| AU4969996A (en) | 1996-08-21 |
| JP2007190028A (ja) | 2007-08-02 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |