ATE192196T1 - Vorbereitung von nukleinsäure aus mononuklearen zellen - Google Patents
Vorbereitung von nukleinsäure aus mononuklearen zellenInfo
- Publication number
- ATE192196T1 ATE192196T1 AT93304440T AT93304440T ATE192196T1 AT E192196 T1 ATE192196 T1 AT E192196T1 AT 93304440 T AT93304440 T AT 93304440T AT 93304440 T AT93304440 T AT 93304440T AT E192196 T1 ATE192196 T1 AT E192196T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acid
- cells
- preparation
- mononuclear cells
- acid sequence
- Prior art date
Links
- 150000007523 nucleic acids Chemical class 0.000 title abstract 7
- 108020004707 nucleic acids Proteins 0.000 title abstract 5
- 102000039446 nucleic acids Human genes 0.000 title abstract 5
- 210000005087 mononuclear cell Anatomy 0.000 title abstract 2
- 210000004027 cell Anatomy 0.000 abstract 6
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 2
- VTLYFUHAOXGGBS-UHFFFAOYSA-N Fe3+ Chemical compound [Fe+3] VTLYFUHAOXGGBS-UHFFFAOYSA-N 0.000 abstract 1
- 230000003321 amplification Effects 0.000 abstract 1
- 238000005119 centrifugation Methods 0.000 abstract 1
- 239000008139 complexing agent Substances 0.000 abstract 1
- 229910001447 ferric ion Inorganic materials 0.000 abstract 1
- 230000002934 lysing effect Effects 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 230000003612 virological effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US89558792A | 1992-06-08 | 1992-06-08 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE192196T1 true ATE192196T1 (de) | 2000-05-15 |
Family
ID=25404721
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT93304440T ATE192196T1 (de) | 1992-06-08 | 1993-06-08 | Vorbereitung von nukleinsäure aus mononuklearen zellen |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US5639599A (de) |
| EP (1) | EP0574227B1 (de) |
| JP (1) | JP3494646B2 (de) |
| KR (1) | KR950701979A (de) |
| AT (1) | ATE192196T1 (de) |
| AU (2) | AU687352B2 (de) |
| CA (1) | CA2137563C (de) |
| DE (1) | DE69328459T2 (de) |
| DK (1) | DK0574227T3 (de) |
| ES (1) | ES2145030T3 (de) |
| WO (1) | WO1993025710A1 (de) |
Families Citing this family (44)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5714380A (en) | 1986-10-23 | 1998-02-03 | Amoco Corporation | Closed vessel for isolating target molecules and for performing amplification |
| US5789148A (en) * | 1994-08-31 | 1998-08-04 | Dendreon Corporation | Cell separation composition |
| US5582988A (en) * | 1994-09-15 | 1996-12-10 | Johnson & Johnson Clinical Diagnostics, Inc. | Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same |
| US5571673A (en) * | 1994-11-23 | 1996-11-05 | Hoffmann-La Roche Inc. | Methods for in-solution quenching of fluorescently labeled oligonucleotide probes |
| WO1997021488A1 (en) * | 1995-12-11 | 1997-06-19 | Dendreon Corporation | Cell separation composition, kit and method |
| US20050287583A1 (en) * | 1997-01-21 | 2005-12-29 | Promega Corporation | Methods and kits for isolating biological target materials using silica magnetic particles |
| US6027945A (en) * | 1997-01-21 | 2000-02-22 | Promega Corporation | Methods of isolating biological target materials using silica magnetic particles |
| US7632651B2 (en) | 1997-09-15 | 2009-12-15 | Mds Analytical Technologies (Us) Inc. | Molecular modification assays |
| US7745142B2 (en) | 1997-09-15 | 2010-06-29 | Molecular Devices Corporation | Molecular modification assays |
| US7070921B2 (en) | 2000-04-28 | 2006-07-04 | Molecular Devices Corporation | Molecular modification assays |
| US8337753B2 (en) | 1998-05-01 | 2012-12-25 | Gen-Probe Incorporated | Temperature-controlled incubator having a receptacle mixing mechanism |
| DE69940647D1 (de) | 1998-05-01 | 2009-05-07 | Gen Probe Inc | Automatische diagnostische Analysevorrichtung |
| US6268127B1 (en) * | 1999-02-03 | 2001-07-31 | Ortho-Clinical Diagnostics, Inc. | Method for preparing DNA from serum and plasma |
| US7297485B2 (en) * | 2001-10-15 | 2007-11-20 | Qiagen Gmbh | Method for nucleic acid amplification that results in low amplification bias |
| US7399590B2 (en) | 2002-02-21 | 2008-07-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| US8030000B2 (en) * | 2002-02-21 | 2011-10-04 | Alere San Diego, Inc. | Recombinase polymerase amplification |
| US9487823B2 (en) | 2002-12-20 | 2016-11-08 | Qiagen Gmbh | Nucleic acid amplification |
| US7955795B2 (en) * | 2003-06-06 | 2011-06-07 | Qiagen Gmbh | Method of whole genome amplification with reduced artifact production |
| US8043834B2 (en) * | 2003-03-31 | 2011-10-25 | Qiagen Gmbh | Universal reagents for rolling circle amplification and methods of use |
| CA2526409A1 (en) * | 2003-05-19 | 2004-12-02 | Brandeis University | Nucleic acid processing methods, kits and devices |
| US20050130177A1 (en) | 2003-12-12 | 2005-06-16 | 3M Innovative Properties Company | Variable valve apparatus and methods |
| US7939249B2 (en) * | 2003-12-24 | 2011-05-10 | 3M Innovative Properties Company | Methods for nucleic acid isolation and kits using a microfluidic device and concentration step |
| CA2898814C (en) | 2004-08-27 | 2015-12-29 | Gen-Probe Incorporated | Single-primer nucleic acid amplification methods |
| US7794659B2 (en) | 2005-03-10 | 2010-09-14 | Gen-Probe Incorporated | Signal measuring system having a movable signal measuring device |
| EP1921454B1 (de) | 2005-03-10 | 2015-08-12 | Gen-Probe Incorporated | Systeme und Verfahren zur Durchführung von Tests zum Nachweis oder zur Quantifizierung von Analyten |
| EP1863908B1 (de) | 2005-04-01 | 2010-11-17 | Qiagen GmbH | Reverse transkription und amplifikation von rna bei simultaner degradierung von dna |
| EP1762627A1 (de) | 2005-09-09 | 2007-03-14 | Qiagen GmbH | Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion |
| EP1978111B1 (de) | 2007-04-02 | 2013-03-27 | Gen-Probe Incorporated | Zusammensetzungen, Kits und zugehörige Verfahren zur Erkennung und/oder Überwachung von Pseudomonas aeruginosa |
| US8357538B2 (en) * | 2007-04-06 | 2013-01-22 | Qiagen Gaithersburg, Inc. | Automated assay and system |
| US8703492B2 (en) * | 2007-04-06 | 2014-04-22 | Qiagen Gaithersburg, Inc. | Open platform hybrid manual-automated sample processing system |
| AU2009262870B2 (en) | 2008-05-30 | 2014-03-20 | Gen-Probe Incorporated | Compositions, kits and related methods for the detection and/or monitoring of Salmonella |
| EP2910647A1 (de) | 2008-12-30 | 2015-08-26 | Gen-Probe Incorporated | Zusammensetzungen, Kits und damit verbundene Verfahren zum Nachweis und/oder Überwachen von Listerien |
| US8039613B2 (en) | 2009-08-28 | 2011-10-18 | Promega Corporation | Methods of purifying a nucleic acid and formulation and kit for use in performing such methods |
| US8222397B2 (en) * | 2009-08-28 | 2012-07-17 | Promega Corporation | Methods of optimal purification of nucleic acids and kit for use in performing such methods |
| US9953141B2 (en) | 2009-11-18 | 2018-04-24 | Becton, Dickinson And Company | Laboratory central control unit method and system |
| KR20130029128A (ko) | 2010-07-23 | 2013-03-21 | 베크만 컬터, 인코포레이티드 | 실시간 pcr용 용기 |
| KR20140091033A (ko) | 2011-11-07 | 2014-07-18 | 베크만 컬터, 인코포레이티드 | 검체 컨테이너 검출 |
| CN104040352B (zh) | 2011-11-07 | 2016-09-07 | 贝克曼考尔特公司 | 机械臂 |
| EP3373015A1 (de) | 2011-11-07 | 2018-09-12 | Beckman Coulter Inc. | Aliquotierungssystem und arbeitsablauf |
| US9910054B2 (en) | 2011-11-07 | 2018-03-06 | Beckman Coulter, Inc. | System and method for processing samples |
| US8973736B2 (en) | 2011-11-07 | 2015-03-10 | Beckman Coulter, Inc. | Magnetic damping for specimen transport system |
| WO2013070755A2 (en) | 2011-11-07 | 2013-05-16 | Beckman Coulter, Inc. | Centrifuge system and workflow |
| GB201618106D0 (en) * | 2016-10-26 | 2016-12-07 | Lift Biosciences Ltd | Cancer-killing cells |
| US10427162B2 (en) | 2016-12-21 | 2019-10-01 | Quandx Inc. | Systems and methods for molecular diagnostics |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3852424A (en) * | 1957-07-26 | 1974-12-03 | Ciba Geigy Corp | Purified ferrimycin and process for obtaining same |
| US3957435A (en) * | 1974-12-12 | 1976-05-18 | Miles Laboratories, Inc. | Passive hemagglutination test method and composition for use therein |
| US4767708A (en) | 1984-08-07 | 1988-08-30 | Carnegie Mellon University | Enzyme amplification and purification |
| US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4863964A (en) * | 1985-07-02 | 1989-09-05 | Biomedical Frontiers, Inc. | Method for the stabilization of deferoxamine to chelate free ions in physiological fluid |
| US4994368A (en) * | 1987-07-23 | 1991-02-19 | Syntex (U.S.A.) Inc. | Amplification method for polynucleotide assays |
| WO1989002476A1 (en) * | 1987-09-21 | 1989-03-23 | Ml Technology Ventures, L.P. | Homogeneous protection assay |
| EP0338591A3 (de) * | 1988-04-21 | 1991-09-04 | Microprobe Corporation | Verfahren zum Extrahieren vom Nukleinsäuren |
| CA2020958C (en) * | 1989-07-11 | 2005-01-11 | Daniel L. Kacian | Nucleic acid sequence amplification methods |
| WO1991001384A1 (en) * | 1989-07-11 | 1991-02-07 | Gen-Probe, Incorporated | Nucleic acid sequence amplification methods utilizing a transcription complex |
| US5284940A (en) * | 1990-11-14 | 1994-02-08 | Hri Research, Inc. | Preparation for nucleic acid samples |
| CA2115061A1 (en) * | 1991-08-06 | 1993-02-18 | David S. Sigman | Method of isolation of dna |
-
1993
- 1993-06-07 WO PCT/US1993/005472 patent/WO1993025710A1/en not_active Ceased
- 1993-06-07 AU AU45308/93A patent/AU687352B2/en not_active Ceased
- 1993-06-07 KR KR1019940704480A patent/KR950701979A/ko not_active Withdrawn
- 1993-06-07 CA CA002137563A patent/CA2137563C/en not_active Expired - Fee Related
- 1993-06-07 JP JP50167494A patent/JP3494646B2/ja not_active Expired - Fee Related
- 1993-06-08 DK DK93304440T patent/DK0574227T3/da active
- 1993-06-08 DE DE69328459T patent/DE69328459T2/de not_active Expired - Fee Related
- 1993-06-08 ES ES93304440T patent/ES2145030T3/es not_active Expired - Lifetime
- 1993-06-08 AT AT93304440T patent/ATE192196T1/de not_active IP Right Cessation
- 1993-06-08 EP EP93304440A patent/EP0574227B1/de not_active Expired - Lifetime
-
1994
- 1994-06-06 US US08/254,310 patent/US5639599A/en not_active Expired - Lifetime
-
1998
- 1998-01-15 AU AU52076/98A patent/AU713753B2/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| CA2137563C (en) | 2005-06-28 |
| EP0574227A2 (de) | 1993-12-15 |
| DE69328459D1 (de) | 2000-05-31 |
| KR950701979A (ko) | 1995-05-17 |
| JPH07507458A (ja) | 1995-08-24 |
| AU713753B2 (en) | 1999-12-09 |
| EP0574227B1 (de) | 2000-04-26 |
| JP3494646B2 (ja) | 2004-02-09 |
| US5639599A (en) | 1997-06-17 |
| ES2145030T3 (es) | 2000-07-01 |
| EP0574227A3 (en) | 1994-08-24 |
| AU687352B2 (en) | 1998-02-26 |
| AU5207698A (en) | 1998-04-23 |
| AU4530893A (en) | 1994-01-04 |
| AU5207498A (en) | 1998-03-12 |
| DK0574227T3 (da) | 2000-08-07 |
| CA2137563A1 (en) | 1993-12-23 |
| AU706083B2 (en) | 1999-06-10 |
| DE69328459T2 (de) | 2000-09-07 |
| WO1993025710A1 (en) | 1993-12-23 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification | ||
| REN | Ceased due to non-payment of the annual fee |