ATE242780T1 - Verfahren zur isolierung von nukleinsäure und vorrichtung hierfür - Google Patents

Verfahren zur isolierung von nukleinsäure und vorrichtung hierfür

Info

Publication number
ATE242780T1
ATE242780T1 AT99947774T AT99947774T ATE242780T1 AT E242780 T1 ATE242780 T1 AT E242780T1 AT 99947774 T AT99947774 T AT 99947774T AT 99947774 T AT99947774 T AT 99947774T AT E242780 T1 ATE242780 T1 AT E242780T1
Authority
AT
Austria
Prior art keywords
nucleic acid
filter
retentate
retained
isolation
Prior art date
Application number
AT99947774T
Other languages
English (en)
Inventor
Andrew Martin Mitchell
Neil James Butt
Christopher Peter Jones
Original Assignee
Whatman Bioscience Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9822141.9A external-priority patent/GB9822141D0/en
Application filed by Whatman Bioscience Ltd filed Critical Whatman Bioscience Ltd
Application granted granted Critical
Publication of ATE242780T1 publication Critical patent/ATE242780T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT99947774T 1998-10-09 1999-10-08 Verfahren zur isolierung von nukleinsäure und vorrichtung hierfür ATE242780T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB9822141.9A GB9822141D0 (en) 1998-10-09 1998-10-09 Isolation method and apparatus
GB9918193A GB2346370B (en) 1998-10-09 1999-08-02 Nucleic acid isolation method and apparatus
PCT/GB1999/003357 WO2000021973A1 (en) 1998-10-09 1999-10-08 Isolation method for nucleic acid and apparatus

Publications (1)

Publication Number Publication Date
ATE242780T1 true ATE242780T1 (de) 2003-06-15

Family

ID=26314493

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99947774T ATE242780T1 (de) 1998-10-09 1999-10-08 Verfahren zur isolierung von nukleinsäure und vorrichtung hierfür

Country Status (6)

Country Link
US (2) US20020010323A1 (de)
EP (1) EP1119576B1 (de)
AT (1) ATE242780T1 (de)
AU (1) AU6114199A (de)
DE (1) DE69908795T2 (de)
WO (1) WO2000021973A1 (de)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6844158B1 (en) * 1997-12-22 2005-01-18 Hitachi Chemical Co., Ltd. Direct RT-PCR on oligonucleotide-immobilized PCR microplates
US6958392B2 (en) 1998-10-09 2005-10-25 Whatman, Inc. Methods for the isolation of nucleic acids and for quantitative DNA extraction and detection for leukocyte evaluation in blood products
US20030170669A1 (en) * 2000-04-11 2003-09-11 Garvin Alex M. Method of nucleic acid recovery
GB2363381B (en) 2000-06-12 2004-11-24 Cambridge Molecular Tech Solution
WO2002014560A1 (en) * 2000-08-16 2002-02-21 Whatman, Inc. Transfusion medicine leukodepletion filter devices as a source of genetic material for genotyping studies
WO2002066637A2 (en) * 2000-10-31 2002-08-29 Hitachi Chemical Research Center, Inc. Method for collecting and using nuclear mrna
US7285199B2 (en) * 2000-10-31 2007-10-23 Hitachi Chemical Research Center, Inc. Apparatus and method for electrophoretic microspot concentration
US20030003600A1 (en) * 2001-07-02 2003-01-02 Fuji Photo Film Co., Ltd. Biochemical analysis kit and method for exposing stimulable phosphor sheet
CN100487116C (zh) * 2001-07-09 2009-05-13 旭化成株式会社 应用非织造织物纯化和检测核酸的方法
GB0129662D0 (en) 2001-12-11 2002-01-30 Whatman Internat Ltd Stabilisation of double-stranded nucleic acids
FR2838066B1 (fr) * 2002-04-09 2004-06-25 Genesystems Procede et automate d'extraction d'acides nucleiques a partir d'un melange complexe
US7745180B2 (en) 2002-04-24 2010-06-29 Hitachi Chemical Co., Ltd. Device and method for high-throughput quantification of mRNA from whole blood
WO2003091407A2 (en) * 2002-04-24 2003-11-06 Hitachi Chemical Research Center, Inc. Device and method for high-throughput quantification of mrna from whole blood
US20060094015A1 (en) * 2002-10-04 2006-05-04 Whatman Inc. Methods and materials for using chemical compounds as a tool for nucleic acid storage on media of nucleic acid purification systems
US20050227225A1 (en) * 2004-04-07 2005-10-13 Roche Molecular Systems, Inc. Stabilization of biomolecules in samples
DE102005047736B4 (de) * 2005-09-29 2008-08-14 Aj Innuscreen Gmbh Verfahren und System zur Isolierung von Nukleinsäuren aus beliebigen komplexen Ausgangsmaterialien
US9428746B2 (en) 2007-10-31 2016-08-30 Akonni Biosystems, Inc. Method and kit for purifying nucleic acids
US7759112B2 (en) * 2007-10-31 2010-07-20 Akonni Biosystems, Inc. Apparatus, system, and method for purifying nucleic acids
US10125388B2 (en) 2007-10-31 2018-11-13 Akonni Biosystems, Inc. Integrated sample processing system
US20090111193A1 (en) 2007-10-31 2009-04-30 Cooney Christopher G Sample preparation device
CN103173432B (zh) 2011-12-22 2020-08-04 通用电气公司 分离核酸的方法及装置
KR101915675B1 (ko) 2012-02-07 2018-11-06 주식회사 미코바이오메드 초고속 핵산 추출 장치, 및 이를 이용하는 핵산 추출 방법
WO2014151177A1 (en) 2013-03-15 2014-09-25 3M Innovative Properties Company Sample concentrator and method of use
EP3394293B1 (de) * 2015-12-22 2021-05-26 Canon U.S. Life Sciences, Inc. Sample-to-answer-system zum nachweis von mikroorganismen mit zielanreicherung, amplifizierung und nachweis
CN105624152B (zh) * 2016-03-01 2019-02-19 中国人民解放军第二军医大学 一种用于核酸扩增的酵母样真菌总dna无仪器提取方法
GB201703383D0 (en) 2017-03-02 2017-04-19 Gargle Tech Ltd Testing for particulates
EP3619533B1 (de) 2017-05-05 2022-09-28 bioMérieux Erkennungsverfahren einer zellulären immunantwort
KR102114148B1 (ko) * 2018-01-03 2020-05-22 재단법인 구미전자정보기술원 식품으로부터 미생물을 검출하는 방법
EP3846939B1 (de) 2018-09-05 2023-05-03 Hero Scientific Ltd Prüfung auf feinstoffteilchen
EP4118412A1 (de) 2020-03-11 2023-01-18 Hero Scientific Ltd Prüfvorrichtungen
US20230098151A1 (en) * 2020-03-11 2023-03-30 Hero Scientific Ltd. Testing for viruses and cellular biomarkers
EP4165385B1 (de) 2021-01-06 2024-04-17 Hero Scientific Ltd Vorrichtungen zur probenentnahme durch filtration
KR102412078B1 (ko) 2021-12-23 2022-06-22 홍승범 다공성 이온 전하 결정체를 이용한 핵산 추출방법

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3639949A1 (de) * 1986-11-22 1988-06-09 Diagen Inst Molekularbio Verfahren zur trennung von langkettigen nukleinsaeuren
US5652141A (en) * 1990-10-26 1997-07-29 Oiagen Gmbh Device and process for isolating nucleic acids from cell suspension
DE4034036C2 (de) * 1990-10-26 1994-03-03 Diagen Inst Molekularbio Vorrichtung und Verfahren zur Isolierung von Nukleinsäuren aus Zellsuspensionen
US5234824A (en) * 1990-11-13 1993-08-10 Specialty Laboratories, Inc. Rapid purification of DNA
US5187083A (en) * 1990-11-13 1993-02-16 Specialty Laboratories, Inc. Rapid purification of DNA
DE69719409T2 (de) * 1996-06-18 2004-01-15 Rikagaku Kenkyusho Verfahren zur Reinigung von DNS
AU730718B2 (en) * 1996-09-18 2001-03-15 Promega Corporation Improved filtration system and method

Also Published As

Publication number Publication date
DE69908795T2 (de) 2004-04-22
EP1119576A1 (de) 2001-08-01
WO2000021973A1 (en) 2000-04-20
AU6114199A (en) 2000-05-01
US20040054163A1 (en) 2004-03-18
EP1119576B1 (de) 2003-06-11
US20020010323A1 (en) 2002-01-24
DE69908795D1 (de) 2003-07-17

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