ATE194015T1 - Verfahren zur entkoppelten, direkten, exponentiellen amplifikation und sequenzierung von dna-molekülen unter zugabe einer zweiten thermostabile dna-polymerase und ihre anwendung - Google Patents

Verfahren zur entkoppelten, direkten, exponentiellen amplifikation und sequenzierung von dna-molekülen unter zugabe einer zweiten thermostabile dna-polymerase und ihre anwendung

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Publication number
ATE194015T1
ATE194015T1 AT97122379T AT97122379T ATE194015T1 AT E194015 T1 ATE194015 T1 AT E194015T1 AT 97122379 T AT97122379 T AT 97122379T AT 97122379 T AT97122379 T AT 97122379T AT E194015 T1 ATE194015 T1 AT E194015T1
Authority
AT
Austria
Prior art keywords
dna polymerase
sequencing
thermostable dna
decoupling
direct
Prior art date
Application number
AT97122379T
Other languages
English (en)
Inventor
Svante Erik Prof Dr Paeaebo
Christian Alexis Dr Kilger
Original Assignee
Roche Diagnostics Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Roche Diagnostics Gmbh filed Critical Roche Diagnostics Gmbh
Application granted granted Critical
Publication of ATE194015T1 publication Critical patent/ATE194015T1/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT97122379T 1996-12-20 1997-12-18 Verfahren zur entkoppelten, direkten, exponentiellen amplifikation und sequenzierung von dna-molekülen unter zugabe einer zweiten thermostabile dna-polymerase und ihre anwendung ATE194015T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19653494A DE19653494A1 (de) 1996-12-20 1996-12-20 Verfahren zur entkoppelten, direkten, exponentiellen Amplifikation und Sequenzierung von DNA Molekülen unter Zugabe einer zweiten thermostabilen DNA Polymerase und dessen Anwendung

Publications (1)

Publication Number Publication Date
ATE194015T1 true ATE194015T1 (de) 2000-07-15

Family

ID=7815667

Family Applications (1)

Application Number Title Priority Date Filing Date
AT97122379T ATE194015T1 (de) 1996-12-20 1997-12-18 Verfahren zur entkoppelten, direkten, exponentiellen amplifikation und sequenzierung von dna-molekülen unter zugabe einer zweiten thermostabile dna-polymerase und ihre anwendung

Country Status (5)

Country Link
US (3) US6225092B1 (de)
EP (1) EP0854196B1 (de)
JP (1) JPH10225297A (de)
AT (1) ATE194015T1 (de)
DE (2) DE19653494A1 (de)

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JP2000516468A (ja) 1996-08-14 2000-12-12 ライフ テクノロジーズ，インコーポレイテッド 核酸増幅および配列決定のための安定な組成物
US6828094B2 (en) * 1996-12-20 2004-12-07 Roche Diagnostics Gmbh Method for the uncoupled, direct, exponential amplification and sequencing of DNA molecules with the addition of a second thermostable DNA polymerase and its application
CA2283789C (en) * 1997-03-12 2005-07-05 Applied Biosystems, Llc Dna polymerases having improved labeled nucleotide incorporation properties
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6605434B1 (en) * 1999-03-16 2003-08-12 Human Genome Sciences, Inc. Direct bacterial lysate sequencing
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
CN1336960A (zh) * 1999-11-26 2002-02-20 株式会社百尼尔 采用多种dna聚合酶的dna测序方法和所用的试剂盒
US6436677B1 (en) * 2000-03-02 2002-08-20 Promega Corporation Method of reverse transcription
US6436641B1 (en) * 2000-04-17 2002-08-20 Visible Genetics Inc. Method and apparatus for DNA sequencing
WO2002072892A1 (en) 2001-03-12 2002-09-19 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
US20050042629A1 (en) 2002-09-13 2005-02-24 Applera Corporation Thermus scotoductus nucleic acid polymerases
US20030219770A1 (en) * 2001-11-08 2003-11-27 Eshleman James R. Methods and systems of nucleic acid sequencing
AU2002346498A1 (en) 2001-11-30 2003-06-17 Applera Corporation Thermus brockianus nucleic acid polymerases
WO2003068991A1 (en) * 2002-02-12 2003-08-21 Applera Corporation Polymerase compositions
WO2003071252A2 (en) * 2002-02-15 2003-08-28 Exact Sciences Corporation Methods for analysis of molecular events
AU2003269991A1 (en) * 2003-08-20 2005-03-10 Applera Corporation Polymerase compositions
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
EP2248911A1 (de) 2004-02-19 2010-11-10 Helicos Biosciences Corporation Verfahren und Zusammensetzungen zur Analyse von Polynukleotidsequenzen
US20080241827A1 (en) * 2004-05-10 2008-10-02 Exact Sciences Corporation Methods For Detecting A Mutant Nucleic Acid
US7476734B2 (en) 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
CA2566806A1 (en) 2004-05-25 2006-01-19 Helicos Biosciences Corporation Methods and devices for nucleic acid sequence determination
US7981607B2 (en) * 2004-08-27 2011-07-19 Esoterix Genetic Laboratories LLC Method for detecting recombinant event
EP1634965B1 (de) 2004-09-09 2010-01-20 Roche Diagnostics GmbH Echtzeit-PCR unter Zusatz von Pyrophosphatase
US20060051796A1 (en) * 2004-09-09 2006-03-09 Inga Boell Real time PCR with the addition of pyrophosphatase
US9109256B2 (en) 2004-10-27 2015-08-18 Esoterix Genetic Laboratories, Llc Method for monitoring disease progression or recurrence
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US7482120B2 (en) 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
WO2007044071A2 (en) * 2005-04-21 2007-04-19 Exact Sciences Corporation Analysis of heterogeneous nucleic acid samples
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US7397546B2 (en) 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam
US20120142004A1 (en) * 2009-05-21 2012-06-07 Siemens Healthcare Diagnostics Inc. Universal Tags With Non-Natural Nucleobases
US9617588B2 (en) * 2010-10-05 2017-04-11 Thermo Fisher Scientific Baltics Uab Enzyme mixture
WO2014090836A1 (en) 2012-12-13 2014-06-19 Roche Diagnostics Gmbh Dna polymerases with improved activity

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US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5693517A (en) * 1987-06-17 1997-12-02 Roche Molecular Systems, Inc. Reagents and methods for coupled high temperature reverse transcription and polymerase chain reactions
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Also Published As

Publication number Publication date
DE69702333D1 (de) 2000-07-27
US20020177129A1 (en) 2002-11-28
US6225092B1 (en) 2001-05-01
EP0854196A1 (de) 1998-07-22
JPH10225297A (ja) 1998-08-25
DE19653494A1 (de) 1998-06-25
US20040146911A1 (en) 2004-07-29
EP0854196B1 (de) 2000-06-21
DE69702333T2 (de) 2000-12-14

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