ATE194015T1 - Verfahren zur entkoppelten, direkten, exponentiellen amplifikation und sequenzierung von dna-molekülen unter zugabe einer zweiten thermostabile dna-polymerase und ihre anwendung - Google Patents

Verfahren zur entkoppelten, direkten, exponentiellen amplifikation und sequenzierung von dna-molekülen unter zugabe einer zweiten thermostabile dna-polymerase und ihre anwendung

Info

Publication number
ATE194015T1
ATE194015T1 AT97122379T AT97122379T ATE194015T1 AT E194015 T1 ATE194015 T1 AT E194015T1 AT 97122379 T AT97122379 T AT 97122379T AT 97122379 T AT97122379 T AT 97122379T AT E194015 T1 ATE194015 T1 AT E194015T1
Authority
AT
Austria
Prior art keywords
dna polymerase
sequencing
thermostable dna
decoupling
direct
Prior art date
Application number
AT97122379T
Other languages
English (en)
Inventor
Svante Erik Prof Dr Paeaebo
Christian Alexis Dr Kilger
Original Assignee
Roche Diagnostics Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics Gmbh filed Critical Roche Diagnostics Gmbh
Application granted granted Critical
Publication of ATE194015T1 publication Critical patent/ATE194015T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT97122379T 1996-12-20 1997-12-18 Verfahren zur entkoppelten, direkten, exponentiellen amplifikation und sequenzierung von dna-molekülen unter zugabe einer zweiten thermostabile dna-polymerase und ihre anwendung ATE194015T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19653494A DE19653494A1 (de) 1996-12-20 1996-12-20 Verfahren zur entkoppelten, direkten, exponentiellen Amplifikation und Sequenzierung von DNA Molekülen unter Zugabe einer zweiten thermostabilen DNA Polymerase und dessen Anwendung

Publications (1)

Publication Number Publication Date
ATE194015T1 true ATE194015T1 (de) 2000-07-15

Family

ID=7815667

Family Applications (1)

Application Number Title Priority Date Filing Date
AT97122379T ATE194015T1 (de) 1996-12-20 1997-12-18 Verfahren zur entkoppelten, direkten, exponentiellen amplifikation und sequenzierung von dna-molekülen unter zugabe einer zweiten thermostabile dna-polymerase und ihre anwendung

Country Status (5)

Country Link
US (3) US6225092B1 (de)
EP (1) EP0854196B1 (de)
JP (1) JPH10225297A (de)
AT (1) ATE194015T1 (de)
DE (2) DE19653494A1 (de)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2264045B1 (de) 1996-08-14 2015-10-21 Life Technologies Corporation Stabile Zusammensetzungen zur Amplifizierung und Sequenzierung von Nukleinsäuren
US6828094B2 (en) * 1996-12-20 2004-12-07 Roche Diagnostics Gmbh Method for the uncoupled, direct, exponential amplification and sequencing of DNA molecules with the addition of a second thermostable DNA polymerase and its application
DE69841023D1 (de) * 1997-03-12 2009-09-10 Applied Biosystems Llc DNA Polymerasen mit verbesserten Fähigkeiten zum Einbau von markierten Nukleotiden
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6605434B1 (en) * 1999-03-16 2003-08-12 Human Genome Sciences, Inc. Direct bacterial lysate sequencing
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
WO2001038574A1 (en) * 1999-11-26 2001-05-31 Bioneer Corporation Dna sequencing method which employs various dna polymerases and kit used for the same
US6436677B1 (en) * 2000-03-02 2002-08-20 Promega Corporation Method of reverse transcription
US6436641B1 (en) * 2000-04-17 2002-08-20 Visible Genetics Inc. Method and apparatus for DNA sequencing
JP2004523243A (ja) 2001-03-12 2004-08-05 カリフォルニア インスティチュート オブ テクノロジー 非同期性塩基伸長によってポリヌクレオチド配列を分析するための方法および装置
US20050042629A1 (en) 2002-09-13 2005-02-24 Applera Corporation Thermus scotoductus nucleic acid polymerases
WO2003056030A2 (en) * 2001-11-08 2003-07-10 The Johns Hopkins University Methods and systems of nucleic acid sequencing
US7052877B2 (en) 2001-11-30 2006-05-30 Applera Corporation Thermus brockianus nucleic acid polymerases
US20030228589A1 (en) * 2002-02-12 2003-12-11 Bond-Nutter Diane R. Polymerase compositions
US7776524B2 (en) * 2002-02-15 2010-08-17 Genzyme Corporation Methods for analysis of molecular events
WO2005019476A1 (en) * 2003-08-20 2005-03-03 Applera Corporation Polymerase compositions
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
WO2005080605A2 (en) 2004-02-19 2005-09-01 Helicos Biosciences Corporation Methods and kits for analyzing polynucleotide sequences
WO2005111244A2 (en) * 2004-05-10 2005-11-24 Exact Sciences Corporation Methods for detecting a mutant nucleic acid
US7476734B2 (en) 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
US7635562B2 (en) 2004-05-25 2009-12-22 Helicos Biosciences Corporation Methods and devices for nucleic acid sequence determination
WO2006026654A2 (en) 2004-08-27 2006-03-09 Exact Sciences Corporation Method for detecting a recombinant event
EP1634965B1 (de) 2004-09-09 2010-01-20 Roche Diagnostics GmbH Echtzeit-PCR unter Zusatz von Pyrophosphatase
US20060051796A1 (en) * 2004-09-09 2006-03-09 Inga Boell Real time PCR with the addition of pyrophosphatase
US9109256B2 (en) 2004-10-27 2015-08-18 Esoterix Genetic Laboratories, Llc Method for monitoring disease progression or recurrence
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US7482120B2 (en) 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
US9777314B2 (en) * 2005-04-21 2017-10-03 Esoterix Genetic Laboratories, Llc Analysis of heterogeneous nucleic acid samples
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US7397546B2 (en) 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam
EP2432796B1 (de) 2009-05-21 2016-08-17 Siemens Healthcare Diagnostics Inc. Universaltags mit nicht-natürlichen nukleobasen
US9617588B2 (en) * 2010-10-05 2017-04-11 Thermo Fisher Scientific Baltics Uab Enzyme mixture
EP2931884B1 (de) * 2012-12-13 2017-03-08 Roche Diagnostics GmbH Dna polymerase mit verbesserter aktivität

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5693517A (en) * 1987-06-17 1997-12-02 Roche Molecular Systems, Inc. Reagents and methods for coupled high temperature reverse transcription and polymerase chain reactions
US4962020A (en) * 1988-07-12 1990-10-09 President And Fellows Of Harvard College DNA sequencing
US5075216A (en) * 1988-09-23 1991-12-24 Cetus Corporation Methods for dna sequencing with thermus aquaticus dna polymerase
US5427911A (en) 1990-05-01 1995-06-27 Yale University Coupled amplification and sequencing of DNA
WO1993002212A1 (en) * 1991-07-24 1993-02-04 University Partnerships Pty. Ltd. Single step amplification and sequencing of nucleic acids
US5338671A (en) * 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
US5436149A (en) * 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US5432065A (en) * 1993-03-30 1995-07-11 United States Biochemical Corporation Cycle sequencing with non-thermostable DNA polymerases
US5556772A (en) * 1993-12-08 1996-09-17 Stratagene Polymerase compositions and uses thereof
US5512462A (en) * 1994-02-25 1996-04-30 Hoffmann-La Roche Inc. Methods and reagents for the polymerase chain reaction amplification of long DNA sequences
US5912155A (en) * 1994-09-30 1999-06-15 Life Technologies, Inc. Cloned DNA polymerases from Thermotoga neapolitana
US5614365A (en) 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing
US6077664A (en) * 1995-06-07 2000-06-20 Promega Corporation Thermophilic DNA polymerases from Thermotoga neapolitana
US5789168A (en) * 1996-05-01 1998-08-04 Visible Genetics Inc. Method for amplification and sequencing of nucleic acid polymers
US5830657A (en) * 1996-05-01 1998-11-03 Visible Genetics Inc. Method for single-tube sequencing of nucleic acid polymers
US5928906A (en) * 1996-05-09 1999-07-27 Sequenom, Inc. Process for direct sequencing during template amplification

Also Published As

Publication number Publication date
EP0854196B1 (de) 2000-06-21
DE19653494A1 (de) 1998-06-25
US20020177129A1 (en) 2002-11-28
DE69702333D1 (de) 2000-07-27
DE69702333T2 (de) 2000-12-14
US20040146911A1 (en) 2004-07-29
US6225092B1 (en) 2001-05-01
EP0854196A1 (de) 1998-07-22
JPH10225297A (ja) 1998-08-25

Similar Documents

Publication Publication Date Title
ATE194015T1 (de) Verfahren zur entkoppelten, direkten, exponentiellen amplifikation und sequenzierung von dna-molekülen unter zugabe einer zweiten thermostabile dna-polymerase und ihre anwendung
ATE220426T1 (de) Verfahren zur direkten, exponentiellen amplifikation und sequenzierung von dna-molekülen und seine anwendung
ATE331040T1 (de) Zusammensetzungen und verfahren der reversen transkriptase-polymerasekettenreaktion (rt-pcr)
ATE159762T1 (de) Verfahren zur dns-sequenzbestimmung mit dns- polymerase von thermus aquaticus
ATE557083T1 (de) Thermostabiler oder thermoactiver dna-polymerase mit abgeschwächter 3'-5' exonukleaseaktivität
ATE211179T1 (de) Amplifizierung von langen nukleinsäuresequenzen mit pcr
DE69940662D1 (de) Verfahren zur dna-amplifikation und -sequenzierung
ATE114334T1 (de) Chaotropisches verfahren zur schätzung von nukleinen säuren in einer biologischen probe.
ATE360093T1 (de) Reaktionsgemisch und verfahren für rt-pcr mittels einer homopolymeren nukleinsäure
EP1091002A3 (de) Sequentielle Aktivierung einer oder mehrerer enzymatischer Aktivitäten in einer thermozyklischen Reaktion zur Synthese von DNA Molekülen
DE69725076D1 (de) Modifizierte thermostabile DNA Polymerase und eine DNA Polymerasezusammensetzung zur Amplifikation von Nukleinsäuren
DK1040202T3 (da) DNA-polymorfiidentitetsbestemmelse under anvendelse af flowcytometri
DE69523360D1 (de) Isotherme Amplifikation von Nukleinsäuren durch Strangverdrängung
DE69428167D1 (de) Reagenz und Verfahren für reverse Transkription bei hoher Temperatur, verbunden mit einer Polymerase-Kettenreaktion
SG96532A1 (en) Diagnostics and amplification methods overcoming a primer-target mismatch at the primer 3'end
EP0745675A3 (de) Methode zur Amplifizierung von Nukleinsäure und entsprechende Regenzien dafür
KR920700294A (ko) 개선된 프라이머 연장반응
DE3784528D1 (de) Zusammensetzung und verfahren zur stabilisierung von dinukleotiden.
ATE346937T1 (de) Thermostabiles nukleinsäure-polymerase von thermococcus gorgonarius
ATE526418T1 (de) Verbesserte zusammensetzungen zur in-vitro- amplifikation von nukleinsäuren
DE69314469D1 (de) An einem festen Träger reversibel gebundene getrocknete Nucleinsäurehybridisierungsprobe, eine solche Probe verwendendes Verfahren und dafür geeigneter Kit

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties