ATE19790T1 - Verfahren zum enzymatischen ersetzen von b-30 aminosaeuren in insulinen. - Google Patents

Verfahren zum enzymatischen ersetzen von b-30 aminosaeuren in insulinen.

Info

Publication number: ATE19790T1
Authority: AT; Austria
Prior art keywords: amino acid; ins; group; insulin; formula
Prior art date: 1982-01-22

Application number

AT83300309T

Other languages

English (en)

Inventor

Klaus Breddam

Jack Taaning Johansen

Original Assignee

Carlsberg Biotechnology As

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1982-01-22

Filing date

1983-01-21

Publication date

1986-05-15

1983-01-21 Application filed by Carlsberg Biotechnology As filed Critical Carlsberg Biotechnology As

1986-05-15 Application granted granted Critical

1986-05-15 Publication of ATE19790T1 publication Critical patent/ATE19790T1/de

Links

150000001413 amino acids Chemical class 0.000 title abstract 4
150000008575 L-amino acids Chemical class 0.000 abstract 5
-1 amino, hydroxy Chemical group 0.000 abstract 4
NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 abstract 4
102000004877 Insulin Human genes 0.000 abstract 2
108090001061 Insulin Proteins 0.000 abstract 2
125000000217 alkyl group Chemical group 0.000 abstract 2
150000001412 amines Chemical class 0.000 abstract 2
125000003710 aryl alkyl group Chemical group 0.000 abstract 2
125000003118 aryl group Chemical group 0.000 abstract 2
125000001072 heteroaryl group Chemical group 0.000 abstract 2
239000004026 insulin derivative Substances 0.000 abstract 2
108010059841 serine carboxypeptidase Proteins 0.000 abstract 2
239000000758 substrate Substances 0.000 abstract 2
108010059081 Cathepsin A Proteins 0.000 abstract 1
102000005572 Cathepsin A Human genes 0.000 abstract 1
101000976075 Homo sapiens Insulin Proteins 0.000 abstract 1
108010005991 Pork Regular Insulin Proteins 0.000 abstract 1
AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 abstract 1
239000004473 Threonine Substances 0.000 abstract 1
239000007864 aqueous solution Substances 0.000 abstract 1
125000000753 cycloalkyl group Chemical group 0.000 abstract 1
239000006185 dispersion Substances 0.000 abstract 1
229910052736 halogen Inorganic materials 0.000 abstract 1
150000002367 halogens Chemical class 0.000 abstract 1
125000005842 heteroatom Chemical group 0.000 abstract 1
125000000623 heterocyclic group Chemical group 0.000 abstract 1
229910052739 hydrogen Inorganic materials 0.000 abstract 1
239000001257 hydrogen Substances 0.000 abstract 1
125000004435 hydrogen atom Chemical class [H]* 0.000 abstract 1
229940125396 insulin Drugs 0.000 abstract 1
PBGKTOXHQIOBKM-FHFVDXKLSA-N insulin (human) Chemical compound C([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@H]1CSSC[C@H]2C(=O)N[C@H](C(=O)N[C@@H](CO)C(=O)N[C@H](C(=O)N[C@H](C(N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3C=CC(O)=CC=3)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3NC=NC=3)NC(=O)[C@H](CO)NC(=O)CNC1=O)C(=O)NCC(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)NCC(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)O)C(O)=O)C(=O)N[C@@H](CC(N)=O)C(O)=O)=O)CSSC[C@@H](C(N2)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@@H](NC(=O)CN)[C@@H](C)CC)[C@@H](C)CC)[C@@H](C)O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H](NC(=O)[C@@H](N)CC=1C=CC=CC=1)C(C)C)C1=CN=CN1 PBGKTOXHQIOBKM-FHFVDXKLSA-N 0.000 abstract 1
229910021645 metal ion Inorganic materials 0.000 abstract 1
125000004433 nitrogen atom Chemical group N* 0.000 abstract 1
150000003355 serines Chemical class 0.000 abstract 1
125000003396 thiol group Chemical group [H]S* 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/63—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from plants
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Zoology (AREA)
Genetics & Genomics (AREA)
Bioinformatics & Cheminformatics (AREA)
Engineering & Computer Science (AREA)
Wood Science & Technology (AREA)
Biochemistry (AREA)
Molecular Biology (AREA)
General Health & Medical Sciences (AREA)
Medicinal Chemistry (AREA)
Microbiology (AREA)
Biomedical Technology (AREA)
General Engineering & Computer Science (AREA)
Biotechnology (AREA)
Diabetes (AREA)
Mycology (AREA)
Gastroenterology & Hepatology (AREA)
Botany (AREA)
Endocrinology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Biophysics (AREA)
Toxicology (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Peptides Or Proteins (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Enzymes And Modification Thereof (AREA)
Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

AT83300309T 1982-01-22 1983-01-21 Verfahren zum enzymatischen ersetzen von b-30 aminosaeuren in insulinen. ATE19790T1 (de)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
DK27782A DK149824C (da)	1982-01-22	1982-01-22	Fremgangsmaade til enzymatisk udskiftning af b-30 aminosyrer i insuliner
EP83300309A EP0085516B1 (de)	1982-01-22	1983-01-21	Verfahren zum enzymatischen Ersetzen von B-30 Aminosäuren in Insulinen

Publications (1)

Publication Number	Publication Date
ATE19790T1 true ATE19790T1 (de)	1986-05-15

Family

ID=8091736

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT83300309T ATE19790T1 (de)	1982-01-22	1983-01-21	Verfahren zum enzymatischen ersetzen von b-30 aminosaeuren in insulinen.

Country Status (8)

Country	Link
US (1)	US4579820A (de)
EP (1)	EP0085516B1 (de)
AT (1)	ATE19790T1 (de)
AU (1)	AU549272B2 (de)
CA (1)	CA1195940A (de)
DE (1)	DE3363449D1 (de)
DK (1)	DK149824C (de)
ES (1)	ES8402875A1 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE3333640A1 (de) *	1983-09-17	1985-04-25	Hoechst Ag, 6230 Frankfurt	Verfahren zur herstellung von insulin-derivaten, deren b-kette c-terminal verlaengert ist, neue basisch modifizierte insulin-derivate diese enthaltende mittel und ihre verwendung
ZA877505B (en) *	1986-10-14	1989-05-30	Lilly Co Eli	Process for transforming a human insulin precursor to human insulin
BR8803346A (pt) *	1988-06-30	1990-02-13	Biobras Bioquimica Do Brasil S	Processo aperfeicoado para producao em larga escala de cristais de insulina purificada
US5234820A (en) *	1989-06-30	1993-08-10	Board Of Regents Of The University Of Nebraska	Exopeptidase catalyzed site-specific bonding of supports, labels and bioactive agents to proteins
US5279954A (en) *	1989-06-30	1994-01-18	Board Of Regents Of The University Of Nebraska And Bionebraska	Exopeptidase catalyzed site-specific bonding of supports, labels and bioactive agents to proteins
US6187579B1 (en) *	1993-10-28	2001-02-13	Carlsberg A/S	Customized proteases
US5985627A (en) *	1997-02-28	1999-11-16	Carlsberg Laboratory	Modified carboxypeptidase
ATE464390T1 (de)	2001-11-19	2010-04-15	Novo Nordisk As	Verfahren zur herstellung von insulinverbindungen
US7396903B2 (en) *	2001-11-19	2008-07-08	Novo Nordisk A/S	Process for preparing insulin compounds

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US3276961A (en) *	1963-04-08	1966-10-04	Squibb & Sons Inc	Process for preparing human insulin
US4086136A (en) *	1975-10-23	1978-04-25	(Zaidanhojin) Sagami Chemical Research Center	Process for producing a peptide using a serine or thiol proteinase
ZA801929B (en) *	1979-04-06	1981-11-25	Forenede Bryggerier As	A process for enzymatic production of peptides
DE3064479D1 (en) *	1979-04-13	1983-09-08	Shionogi & Co	Process for preparing a b30-threonine insulin
DK147437A (en) *	1980-02-11	1900-01-01		Process for preparing human insulin or threonine B30 esters of human insulin, or a salt or complex thereof

1982
- 1982-01-22 DK DK27782A patent/DK149824C/da not_active IP Right Cessation
1983
- 1983-01-18 US US06/458,847 patent/US4579820A/en not_active Expired - Fee Related
- 1983-01-19 AU AU10608/83A patent/AU549272B2/en not_active Ceased
- 1983-01-20 ES ES519127A patent/ES8402875A1/es not_active Expired
- 1983-01-21 AT AT83300309T patent/ATE19790T1/de active
- 1983-01-21 CA CA000420025A patent/CA1195940A/en not_active Expired
- 1983-01-21 DE DE8383300309T patent/DE3363449D1/de not_active Expired
- 1983-01-21 EP EP83300309A patent/EP0085516B1/de not_active Expired

Also Published As

Publication number	Publication date
DK149824B (da)	1986-10-06
ES519127A0 (es)	1984-03-16
DK149824C (da)	1987-03-16
US4579820A (en)	1986-04-01
AU1060883A (en)	1983-07-28
ES8402875A1 (es)	1984-03-16
CA1195940A (en)	1985-10-29
EP0085516A3 (en)	1983-09-07
DE3363449D1 (en)	1986-06-19
EP0085516B1 (de)	1986-05-14
AU549272B2 (en)	1986-01-23
EP0085516A2 (de)	1983-08-10
DK27782A (da)	1983-07-23

Publication	Publication Date	Title
ES8300858A1 (es)	1982-12-01	Procedimiento de tratamiento de insulinas
AU2507088A (en)	1989-05-25	Amino acid derivatives of phosphorus acids
ES8706167A1 (es)	1987-06-01	Un procedimiento para preparar nuevos peptidos hipotensivos.
DK72587D0 (da)	1987-02-13	Fremgangsmaade til enzymatisk fremstilling af dipeptider
ATE19790T1 (de)	1986-05-15	Verfahren zum enzymatischen ersetzen von b-30 aminosaeuren in insulinen.
ID24668A (id)	1995-08-31	ALKILASI ASAM-ASAM AMINO (Pecahan dari No. P941860)
ATE119202T1 (de)	1995-03-15	Verfahren zur herstellung von l-aminosäuren und aminosäure-amiden.
ATE208378T1 (de)	2001-11-15	Verfahren zur herstellung von nicotinsäuren
ATE43570T1 (de)	1989-06-15	Verfahren zur racemisierung optisch aktiver aminosaeuren.
AT400030B (de)	1995-09-25	Verfahren zur herstellung von 1-alkylamino-2-nitroethen-1-thiolderivaten
DK130082A (da)	1982-03-23	Fremgangsmaade til enzymatisk udskiftning af b-30 aminosyren iinsuliner
US3867424A (en)	1975-02-18	Method for preparing acylated products
ES8505373A1 (es)	1985-06-01	Procedimiento de preparacion de acidos 7-substituido amino-desacetoxicefalosporanicos y sus esteres
KR870003122A (ko)	1987-04-15	3-비닐 세팔로스포린 유도체의 제조방법
ES479301A1 (es)	1979-07-01	Procedimiento de obtencion de sales de acidos aril alcanoi- cos con aminoacidos dibasicos a partir de sales de los mismos.
GB1331714A (en)	1973-09-26	Preparation of peptides
ES438465A3 (es)	1977-02-01	Procedimiento para preparar bencensulfonilureas.
ATE226207T1 (de)	2002-11-15	Verfahren zur herstellung von beta-lactam verbindungen