ATE199168T1 - Stabilisierung von plasmiden - Google Patents

Stabilisierung von plasmiden

Info

Publication number
ATE199168T1
ATE199168T1 AT96929443T AT96929443T ATE199168T1 AT E199168 T1 ATE199168 T1 AT E199168T1 AT 96929443 T AT96929443 T AT 96929443T AT 96929443 T AT96929443 T AT 96929443T AT E199168 T1 ATE199168 T1 AT E199168T1
Authority
AT
Austria
Prior art keywords
repressor
plasmid
gene
expressed
essential
Prior art date
Application number
AT96929443T
Other languages
English (en)
Inventor
David John Sherratt
Steven Geraint Williams
Julian Alexis John Hanak
Original Assignee
Cobra Therapeutics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cobra Therapeutics Ltd filed Critical Cobra Therapeutics Ltd
Application granted granted Critical
Publication of ATE199168T1 publication Critical patent/ATE199168T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/68—Stabilisation of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AT96929443T 1995-09-08 1996-09-06 Stabilisierung von plasmiden ATE199168T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9518395.0A GB9518395D0 (en) 1995-09-08 1995-09-08 Plasmid stabilization
PCT/GB1996/002208 WO1997009435A1 (en) 1995-09-08 1996-09-06 Plasmid stabilization

Publications (1)

Publication Number Publication Date
ATE199168T1 true ATE199168T1 (de) 2001-02-15

Family

ID=10780420

Family Applications (1)

Application Number Title Priority Date Filing Date
AT96929443T ATE199168T1 (de) 1995-09-08 1996-09-06 Stabilisierung von plasmiden

Country Status (14)

Country Link
US (1) US5972708A (de)
EP (1) EP0851932B1 (de)
JP (1) JP3416888B2 (de)
KR (1) KR100356581B1 (de)
AT (1) ATE199168T1 (de)
AU (1) AU710494B2 (de)
CA (1) CA2231784C (de)
DE (1) DE69611794T2 (de)
DK (1) DK0851932T3 (de)
ES (1) ES2155620T3 (de)
GB (1) GB9518395D0 (de)
GR (1) GR3035806T3 (de)
PT (1) PT851932E (de)
WO (1) WO1997009435A1 (de)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5763270A (en) * 1995-06-07 1998-06-09 Genemedicine, Inc. Plasmid for delivery of nucleic acids to cells and methods of use
US6720139B1 (en) 1999-01-27 2004-04-13 Elitra Pharmaceuticals, Inc. Genes identified as required for proliferation in Escherichia coli
US6589738B1 (en) 1999-11-09 2003-07-08 Elitra Pharmaceuticals, Inc. Genes essential for microbial proliferation and antisense thereto
WO2001048209A2 (en) * 1999-12-23 2001-07-05 Elitra Pharmaceuticals, Inc. Genes identified as required for proliferation of e. coli
US6783985B1 (en) 2000-02-18 2004-08-31 Elitra Pharmaceuticals Inc. Gene disruption methodologies for drug target discovery
US20030180953A1 (en) * 2000-12-29 2003-09-25 Elitra Pharmaceuticals, Inc. Gene disruption methodologies for drug target discovery
JP2005522980A (ja) * 2001-04-23 2005-08-04 エリトラ ファーマシューティカルズ，インコーポレイテッド アスペルギルス・フミガーツス（Ａｓｐｅｒｇｉｌｌｕｓｆｕｍｉｇａｔｕｓ）の必須遺伝子の同定および使用方法
US20040029129A1 (en) * 2001-10-25 2004-02-12 Liangsu Wang Identification of essential genes in microorganisms
US20030170694A1 (en) * 2001-12-21 2003-09-11 Daniel Wall Stabilized nucleic acids in gene and drug discovery and methods of use
GB0211459D0 (en) * 2002-05-18 2002-06-26 Cobra Therapeutics Ltd Plasmid stabilisation in vivo
CN1902318A (zh) 2003-10-31 2007-01-24 诺维信公司 细菌宿主细胞中稳定地基因扩增的方法
GB0327056D0 (en) 2003-11-20 2003-12-24 Cobra Biolog Ltd Plasmid maintenance
CA2619989C (en) * 2005-08-23 2014-06-17 National Research Council Of Canada Regulation of heterologous recombinant protein expression in methylotrophic and methanotrophic bacteria
JP2011505147A (ja) * 2007-11-30 2011-02-24 スカラブ ゲノミクス， エルエルシー ｌａｃ発現システム
EP2353607A1 (de) 2010-02-04 2011-08-10 BioAlliance Pharma Verwendung einer Disintegrin-Domäne eines Adamalysins zur Behandlung von Psoriasis
EP3205356A1 (de) 2016-02-10 2017-08-16 Onkoloski Institut Ljubljana Plasmidexpressionsvektor zur codierung von humanem interleukin 12 unter transkriptionskontrolle von p21-promotor und ohne antibiotikaresistenz für krebsgentherapie und andere verwendungen davon
RS61138B1 (sr) * 2016-11-01 2020-12-31 Novo Nordisk As Tolerogena dnk vakcina
US20210346306A1 (en) 2018-05-23 2021-11-11 Modernatx, Inc. Delivery of dna
CN113874509A (zh) * 2019-03-15 2021-12-31 艾力格生物科技有限公司 使用dna结合阻遏物在原核细胞中的转录控制
CA3184474A1 (en) 2020-06-01 2021-12-09 Modernatx, Inc. Phenylalanine hydroxylase variants and uses thereof
EP4337177A1 (de) 2021-05-11 2024-03-20 Modernatx, Inc. Nichtvirale verabreichung von dna für verlängerte polypeptidexpression in vivo

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK410783D0 (da) * 1982-09-16 1983-09-09 Benzon A Salfred Fremgangsmade til stabilisering af plasmider
GB8308236D0 (en) * 1983-03-25 1983-05-05 Celltech Ltd Dna vectors
DK594084A (da) * 1984-12-12 1986-06-13 Novo Industri As Fremgangsmaade til stabilisering af extra-chromosomale elementer i bakterier under dyrkning
GB9215550D0 (en) * 1992-07-22 1992-09-02 Celltech Ltd Protein expression system
US5763270A (en) * 1995-06-07 1998-06-09 Genemedicine, Inc. Plasmid for delivery of nucleic acids to cells and methods of use

Also Published As

Publication number Publication date
PT851932E (pt) 2001-07-31
AU710494B2 (en) 1999-09-23
ES2155620T3 (es) 2001-05-16
EP0851932B1 (de) 2001-02-14
AU6885596A (en) 1997-03-27
DK0851932T3 (da) 2001-06-18
CA2231784C (en) 2010-06-22
WO1997009435A1 (en) 1997-03-13
DE69611794T2 (de) 2001-08-02
KR100356581B1 (ko) 2003-04-10
JP3416888B2 (ja) 2003-06-16
EP0851932A1 (de) 1998-07-08
KR19990044525A (ko) 1999-06-25
GR3035806T3 (en) 2001-07-31
US5972708A (en) 1999-10-26
GB9518395D0 (en) 1995-11-08
JPH11511985A (ja) 1999-10-19
DE69611794D1 (de) 2001-03-22
CA2231784A1 (en) 1997-03-13

Similar Documents

Publication Publication Date Title
ES2155620T3 (es) Estabilizacion de plasmidos.
ES8307297A1 (es) Proceso para la produccion microbiana de interferon fibro- blasto humano
DE69329202D1 (de) Methoden der erhöhung der sekretion von überexpremierten proteinen
AU2790292A (en) Viral recombinant vectors for expression in muscle cells
DK139493A (da) Rekombinant plasmid, bakteriekultur transformeret dermed samt kulturens anvendelse
ATE424410T1 (de) Interleukin-19.
ATE109829T1 (de) Interleukin-7.
HUT58798A (en) Process for producing growth factor of parenchimic hepatic celles, the gen for coding this and transformants for producing this factor
AU3601484A (en) Cloning the gene for rabbit tumor necrosis factor polypeptide
DE3175357D1 (en) Dna sequences encoding various allelic forms of mature thaumatin, recombinant plasmids comprising said dna's and a process for their preparation, bacterial cultures comprising said recombinant plasmids, and method for producing mature thaumatin
ATE254176T1 (de) Erhöhte produktion von beta-galaktosidase in aspergillus oryzae
HU9401604D0 (en) Production of proteins using fb2 protein
EP0164992A3 (de) Regulierende Gene für die Stickstoffixierung
BR8406680A (pt) Processo para preparacao de um plasmidio,processo para expressao eficiente de proteinas heterologas,para preparacao de um transformante e processo para producao de prorrenina
ES8506194A1 (es) Polipeptido de interferon- humano
DE3852870D1 (de) Saurer Fibroblast-Wachstumsfaktor-Mutant.
HU9203434D0 (en) Biosythetic process for producing chemical products
UA27696C2 (uk) Saf-поліпептид, фрагмент днк, що кодує saf-поліпептид, рекомбінантна плазмідна днк для експресії safполіпептиду (варіанти), штам грибів streptomyces lividans, спосіб експресії saf-поліпептиду
AU6698090A (en) Bacterial collagenase gene
ES8706825A1 (es) Un procedimiento para preparar un plasmido recombinante
EP0422217A4 (en) Human recombinant placental ribonuclease inhibitor and method of production
EP0302838A3 (en) Cloning of the gene coding the isoamylase enzyme and its use in the production of said enzyme
JPS6485094A (en) Novel physiologically active polypeptide
JPS6485095A (en) Novel physiologically active polypeptide
AU2371188A (en) Cloning afl ii restriction endonuclease and methylase from anabaena flos-aquae

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification