ATE199168T1 - Stabilisierung von plasmiden - Google Patents

Stabilisierung von plasmiden

Info

Publication number
ATE199168T1
ATE199168T1 AT96929443T AT96929443T ATE199168T1 AT E199168 T1 ATE199168 T1 AT E199168T1 AT 96929443 T AT96929443 T AT 96929443T AT 96929443 T AT96929443 T AT 96929443T AT E199168 T1 ATE199168 T1 AT E199168T1
Authority
AT
Austria
Prior art keywords
repressor
plasmid
gene
expressed
essential
Prior art date
Application number
AT96929443T
Other languages
English (en)
Inventor
David John Sherratt
Steven Geraint Williams
Julian Alexis John Hanak
Original Assignee
Cobra Therapeutics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cobra Therapeutics Ltd filed Critical Cobra Therapeutics Ltd
Application granted granted Critical
Publication of ATE199168T1 publication Critical patent/ATE199168T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/68—Stabilisation of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AT96929443T 1995-09-08 1996-09-06 Stabilisierung von plasmiden ATE199168T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9518395.0A GB9518395D0 (en) 1995-09-08 1995-09-08 Plasmid stabilization
PCT/GB1996/002208 WO1997009435A1 (en) 1995-09-08 1996-09-06 Plasmid stabilization

Publications (1)

Publication Number Publication Date
ATE199168T1 true ATE199168T1 (de) 2001-02-15

Family

ID=10780420

Family Applications (1)

Application Number Title Priority Date Filing Date
AT96929443T ATE199168T1 (de) 1995-09-08 1996-09-06 Stabilisierung von plasmiden

Country Status (14)

Country Link
US (1) US5972708A (de)
EP (1) EP0851932B1 (de)
JP (1) JP3416888B2 (de)
KR (1) KR100356581B1 (de)
AT (1) ATE199168T1 (de)
AU (1) AU710494B2 (de)
CA (1) CA2231784C (de)
DE (1) DE69611794T2 (de)
DK (1) DK0851932T3 (de)
ES (1) ES2155620T3 (de)
GB (1) GB9518395D0 (de)
GR (1) GR3035806T3 (de)
PT (1) PT851932E (de)
WO (1) WO1997009435A1 (de)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5763270A (en) * 1995-06-07 1998-06-09 Genemedicine, Inc. Plasmid for delivery of nucleic acids to cells and methods of use
US6720139B1 (en) 1999-01-27 2004-04-13 Elitra Pharmaceuticals, Inc. Genes identified as required for proliferation in Escherichia coli
US6589738B1 (en) 1999-11-09 2003-07-08 Elitra Pharmaceuticals, Inc. Genes essential for microbial proliferation and antisense thereto
AU2277401A (en) * 1999-12-23 2001-07-09 Elitra Pharmaceuticals, Inc. Genes identified as required for proliferation of e. coli
US6783985B1 (en) 2000-02-18 2004-08-31 Elitra Pharmaceuticals Inc. Gene disruption methodologies for drug target discovery
US20030180953A1 (en) * 2000-12-29 2003-09-25 Elitra Pharmaceuticals, Inc. Gene disruption methodologies for drug target discovery
EP1390468A4 (de) * 2001-04-23 2004-09-22 Elitra Pharmaceuticals Inc Identifizierung essentieller gene von aspergillus fumigatus und verwendungsverfahren
US20040029129A1 (en) * 2001-10-25 2004-02-12 Liangsu Wang Identification of essential genes in microorganisms
US20030170694A1 (en) * 2001-12-21 2003-09-11 Daniel Wall Stabilized nucleic acids in gene and drug discovery and methods of use
GB0211459D0 (en) * 2002-05-18 2002-06-26 Cobra Therapeutics Ltd Plasmid stabilisation in vivo
WO2005042750A1 (en) 2003-10-31 2005-05-12 Novozymes A/S Method for stable gene-amplification in a bacterial host cell
GB0327056D0 (en) * 2003-11-20 2003-12-24 Cobra Biolog Ltd Plasmid maintenance
CA2619989C (en) * 2005-08-23 2014-06-17 National Research Council Of Canada Regulation of heterologous recombinant protein expression in methylotrophic and methanotrophic bacteria
US20110053274A1 (en) * 2007-11-30 2011-03-03 Scarab Genomics Llc Lac expression system
EP2353607A1 (de) 2010-02-04 2011-08-10 BioAlliance Pharma Verwendung einer Disintegrin-Domäne eines Adamalysins zur Behandlung von Psoriasis
EP3205356A1 (de) 2016-02-10 2017-08-16 Onkoloski Institut Ljubljana Plasmidexpressionsvektor zur codierung von humanem interleukin 12 unter transkriptionskontrolle von p21-promotor und ohne antibiotikaresistenz für krebsgentherapie und andere verwendungen davon
CA3042321A1 (en) * 2016-11-01 2018-05-11 Novo Nordisk A/S Tolerogenic dna vaccine
WO2019226650A1 (en) 2018-05-23 2019-11-28 Modernatx, Inc. Delivery of dna
EP3938516A1 (de) * 2019-03-15 2022-01-19 Eligo Bioscience Transkriptionale steuerung in prokaryotischen zellen unter verwendung von dna-bindenden repressoren
EP4158005A1 (de) 2020-06-01 2023-04-05 ModernaTX, Inc. Phenylalaninhydroxylasevarianten und verwendungen davon
US20240263195A1 (en) 2021-05-11 2024-08-08 Modernatx, Inc. Non-viral delivery of dna for prolonged polypeptide expression in vivo

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK410783D0 (da) * 1982-09-16 1983-09-09 Benzon A Salfred Fremgangsmade til stabilisering af plasmider
GB8308236D0 (en) * 1983-03-25 1983-05-05 Celltech Ltd Dna vectors
DK594084A (da) * 1984-12-12 1986-06-13 Novo Industri As Fremgangsmaade til stabilisering af extra-chromosomale elementer i bakterier under dyrkning
GB9215550D0 (en) * 1992-07-22 1992-09-02 Celltech Ltd Protein expression system
US5763270A (en) * 1995-06-07 1998-06-09 Genemedicine, Inc. Plasmid for delivery of nucleic acids to cells and methods of use

Also Published As

Publication number Publication date
EP0851932B1 (de) 2001-02-14
CA2231784A1 (en) 1997-03-13
GR3035806T3 (en) 2001-07-31
KR19990044525A (ko) 1999-06-25
JPH11511985A (ja) 1999-10-19
WO1997009435A1 (en) 1997-03-13
PT851932E (pt) 2001-07-31
JP3416888B2 (ja) 2003-06-16
DK0851932T3 (da) 2001-06-18
AU6885596A (en) 1997-03-27
ES2155620T3 (es) 2001-05-16
DE69611794D1 (de) 2001-03-22
DE69611794T2 (de) 2001-08-02
KR100356581B1 (ko) 2003-04-10
GB9518395D0 (en) 1995-11-08
US5972708A (en) 1999-10-26
EP0851932A1 (de) 1998-07-08
CA2231784C (en) 2010-06-22
AU710494B2 (en) 1999-09-23

Similar Documents

Publication Publication Date Title
ATE199168T1 (de) Stabilisierung von plasmiden
ES8307297A1 (es) Proceso para la produccion microbiana de interferon fibro- blasto humano
DE69329202D1 (de) Methoden der erhöhung der sekretion von überexpremierten proteinen
DK0864645T3 (da) Negativstrenget RNA-virusvektor med autonom replikeringsaktivitet
ES8303529A1 (es) Un procedimiento para la produccion de un plasmido recombi- nante.
ES2009905A6 (es) Procedimiento para la preparacion de anticuerpos frente a virus de sida.
EP0314415A3 (en) Interleukin-7
AU551251B2 (en) Recombinant dna; chymosin production
DK0963377T3 (da) Interleukin-19
EP0176971A3 (de) Für ein thermostabiles Beta-Galaktosidase, kodierendes Gen, dieses Gen enthaltender Bacillus-subtilis, das durch dieses Gen kodiertes Enzym und Verfahren zu dessen Herstellung
HUT58798A (en) Process for producing growth factor of parenchimic hepatic celles, the gen for coding this and transformants for producing this factor
NO892029L (no) Fremgangsmaate ved spesifikk genetisk manipulering av mikroorganisme.
ATE254176T1 (de) Erhöhte produktion von beta-galaktosidase in aspergillus oryzae
HU9401604D0 (en) Production of proteins using fb2 protein
BR8406680A (pt) Processo para preparacao de um plasmidio,processo para expressao eficiente de proteinas heterologas,para preparacao de um transformante e processo para producao de prorrenina
EP0040963A3 (de) Plasmid
ATE238427T1 (de) Vektoren, die therapeutische gene fuer antimikrobielle peptide enthalten, zur verwendung in der gentherapie
AU2430588A (en) Mutant acidic fibroblast growth factor
HU9203434D0 (en) Biosythetic process for producing chemical products
EP0187630A3 (en) Cloned streptomyces beta-galactosidase gene
ES8802251A1 (es) Un procedimiento para preparar un microorganismo hospedante transformado del genero sacharomyces.
HUT63196A (en) Process for producing and applying new cloning and expression vectors
IT8521507A0 (it) Vettore di clonaggio, molecole di dna ricombinante, ceppi di bacillus subtilis trasformati con dette molecole e metodi per l'espressione di geni eterologhi e produzione e secrezione di proteine codificate da detti geni.
JPS6485094A (en) Novel physiologically active polypeptide
JPS6485095A (en) Novel physiologically active polypeptide

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification