ATE199563T1 - 4,7-dichlorrhodaminfarbstoffe - Google Patents

4,7-dichlorrhodaminfarbstoffe

Info

Publication number
ATE199563T1
ATE199563T1 AT97928653T AT97928653T ATE199563T1 AT E199563 T1 ATE199563 T1 AT E199563T1 AT 97928653 T AT97928653 T AT 97928653T AT 97928653 T AT97928653 T AT 97928653T AT E199563 T1 ATE199563 T1 AT E199563T1
Authority
AT
Austria
Prior art keywords
hydrogen
taken together
lower alkyl
benzo
chlorine
Prior art date
Application number
AT97928653T
Other languages
English (en)
Inventor
Linda Lee
Scott C Benson
Barnett B Rosenblum
Sandra L Spungeon
Original Assignee
Perkin Elmer Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Perkin Elmer Corp filed Critical Perkin Elmer Corp
Application granted granted Critical
Publication of ATE199563T1 publication Critical patent/ATE199563T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B11/00—Diaryl- or thriarylmethane dyes
    • C09B11/04—Diaryl- or thriarylmethane dyes derived from triarylmethanes, i.e. central C-atom is substituted by amino, cyano, alkyl
    • C09B11/10—Amino derivatives of triarylmethanes
    • C09B11/24—Phthaleins containing amino groups ; Phthalanes; Fluoranes; Phthalides; Rhodamine dyes; Phthaleins having heterocyclic aryl rings; Lactone or lactame forms of triarylmethane dyes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pyrane Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Paints Or Removers (AREA)
  • Dental Preparations (AREA)
  • Luminescent Compositions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Plural Heterocyclic Compounds (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)
  • Fats And Perfumes (AREA)
  • Inks, Pencil-Leads, Or Crayons (AREA)
AT97928653T 1996-06-27 1997-05-21 4,7-dichlorrhodaminfarbstoffe ATE199563T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/672,196 US5847162A (en) 1996-06-27 1996-06-27 4, 7-Dichlororhodamine dyes
PCT/US1997/008797 WO1997049769A1 (en) 1996-06-27 1997-05-21 4,7-dichlororhodamine dyes

Publications (1)

Publication Number Publication Date
ATE199563T1 true ATE199563T1 (de) 2001-03-15

Family

ID=24697546

Family Applications (1)

Application Number Title Priority Date Filing Date
AT97928653T ATE199563T1 (de) 1996-06-27 1997-05-21 4,7-dichlorrhodaminfarbstoffe

Country Status (8)

Country Link
US (2) US5847162A (de)
EP (1) EP0915935B1 (de)
JP (4) JP4140976B2 (de)
AT (1) ATE199563T1 (de)
AU (1) AU709209B2 (de)
CA (1) CA2258243C (de)
DE (1) DE69704205T2 (de)
WO (1) WO1997049769A1 (de)

Families Citing this family (222)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7388092B2 (en) 1996-05-03 2008-06-17 Applera Corporation Oligonucleotides and analogs labeled with energy transfer dyes
US5945526A (en) * 1996-05-03 1999-08-31 Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US5847162A (en) * 1996-06-27 1998-12-08 The Perkin Elmer Corporation 4, 7-Dichlororhodamine dyes
US7825237B2 (en) * 1996-05-03 2010-11-02 Applied Biosystems, Llc Oligonucleotides and analogs labeled with energy transfer dyes
US6080852A (en) * 1996-06-27 2000-06-27 The Perkin-Elmer Corporation 4,7-dichlororhodamine dyes
US7550570B2 (en) * 2000-05-25 2009-06-23 Applied Biosystems, Llc. 4,7-dichlororhodamine dyes labeled polynucleotides
JP3489991B2 (ja) * 1997-07-07 2004-01-26 理化学研究所 ３’−デオキシリボヌクレオチド誘導体
US5936087A (en) 1997-11-25 1999-08-10 The Perkin-Elmer Corporation Dibenzorhodamine dyes
US6080868A (en) 1998-01-23 2000-06-27 The Perkin-Elmer Corporation Nitro-substituted non-fluorescent asymmetric cyanine dye compounds
DE19824535A1 (de) * 1998-06-03 1999-12-09 Roche Diagnostics Gmbh Neue Rhodamin-Derivate und deren Verwendung
JP3949378B2 (ja) * 1998-08-19 2007-07-25 バイオベンチャーズ， インコーポレイテッド ポリヌクレオチド配列の変異を決定するための方法
US20030138834A1 (en) * 1998-08-19 2003-07-24 Dawson Elliott P. Method for determining polynucleotide sequence variations
US20040121394A1 (en) * 1998-08-19 2004-06-24 Dawson Elliott P. Method for determining polynucleotide sequence variations
US6605434B1 (en) 1999-03-16 2003-08-12 Human Genome Sciences, Inc. Direct bacterial lysate sequencing
DE19923168A1 (de) * 1999-05-20 2000-11-23 Roche Diagnostics Gmbh Neue Fluoreszenzfarbstoffe und ihre Verwendung als Fluoreszenzmarker
US6248884B1 (en) * 1999-06-03 2001-06-19 The Perkin-Elmer Corporation Extended rhodamine compounds useful as fluorescent labels
US6316230B1 (en) * 1999-08-13 2001-11-13 Applera Corporation Polymerase extension at 3′ terminus of PNA-DNA chimera
US6191278B1 (en) 1999-11-03 2001-02-20 Pe Corporation Water-soluble rhodamine dyes and conjugates thereof
US6372907B1 (en) * 1999-11-03 2002-04-16 Apptera Corporation Water-soluble rhodamine dye peptide conjugates
US6232076B1 (en) 2000-02-04 2001-05-15 Genaissance Pharmaceuticals, Inc. Stabilizer of dye sequencing products
US6887664B2 (en) 2000-06-06 2005-05-03 Applera Corporation Asynchronous primed PCR
US7169922B2 (en) 2000-08-04 2007-01-30 Invitrogen Corporation Derivatives of 1,2-dihydro-7-hydroxyquinolines containing fused rings
CA2425663C (en) * 2000-10-11 2009-12-29 Applera Corporation Fluorescent nucleobase conjugates having anionic linkers
US6448407B1 (en) 2000-11-01 2002-09-10 Pe Corporation (Ny) Atropisomers of asymmetric xanthene fluorescent dyes and methods of DNA sequencing and fragment analysis
WO2002055512A1 (en) * 2001-01-12 2002-07-18 Northeastern University Xanthene derivatives
DE60216468T2 (de) * 2001-03-09 2007-09-27 Boston Probes, Inc., Bedford Kombinationsoligomere betreffende verfahren, kits und zusammensetzungen
US6887690B2 (en) * 2001-06-22 2005-05-03 Pe Corporation Dye-labeled ribonucleotide triphosphates
US7052512B2 (en) * 2001-07-18 2006-05-30 Boston Scientific Scimed, Inc. Fluorescent dyed lubricant for medical devices
US6764710B2 (en) 2001-07-18 2004-07-20 Scimed Life Systems, Inc. Light emitting markers for use with substrates
EP1412724B1 (de) * 2001-07-25 2016-03-23 Life Technologies Corporation Zeitverzögerungintegration in elektrophoretischen nachweissystemen
AU2002362013B2 (en) 2001-11-21 2008-04-24 Applied Biosystems, Llc. Digital assay
US20040054195A1 (en) * 2002-01-10 2004-03-18 Jianxin Gao Xanthene derivatives
US7445894B2 (en) 2002-05-03 2008-11-04 Molecular Probes, Inc. Compositions and methods for detection and isolation of phosphorylated molecules
AU2003299466A1 (en) 2002-05-03 2004-06-07 Molecular Probes, Inc. Compositions and methods for detection and isolation of phosphorylated molecules
US20040171034A1 (en) 2002-05-03 2004-09-02 Brian Agnew Compositions and methods for detection and isolation of phosphorylated molecules
EP1575990B1 (de) 2002-09-08 2009-11-18 Applera Corporation Festphasengebundene PNA-Dimere und deren Synthese
US20050233307A1 (en) 2002-09-12 2005-10-20 Kyle Gee Site-specific labeling of affinity tags in fusion proteins
JP2006500065A (ja) * 2002-09-25 2006-01-05 アマシャム・バイオサイエンス・コーポレイション エネルギー移動色素、ダイターミネーター及びその使用
US20050250214A1 (en) * 2004-05-05 2005-11-10 Gee Kyle R Zinc binding compounds and their method of use
US7432298B2 (en) * 2003-05-09 2008-10-07 Applied Biosystems Inc. Fluorescent polymeric materials containing lipid soluble rhodamine dyes
US7491830B2 (en) * 2003-05-09 2009-02-17 Applied Biosystems Inc. Phenyl xanthene dyes
US6998493B2 (en) 2003-06-26 2006-02-14 Xerox Corporation Colorant compounds
US7176317B2 (en) * 2003-06-26 2007-02-13 Xerox Corporation Colorant compounds
US6860931B2 (en) * 2003-06-26 2005-03-01 Xerox Corporation Phase change inks containing colorant compounds
US6835238B1 (en) 2003-06-26 2004-12-28 Xerox Corporation Phase change inks containing colorant compounds
US7619059B2 (en) 2003-07-29 2009-11-17 Life Technologies Corporation Bimolecular optical probes
US7727752B2 (en) 2003-07-29 2010-06-01 Life Technologies Corporation Kinase and phosphatase assays
CA2445420A1 (en) 2003-07-29 2005-01-29 Invitrogen Corporation Kinase and phosphatase assays
US7271265B2 (en) 2003-08-11 2007-09-18 Invitrogen Corporation Cyanine compounds and their application as quenching compounds
ATE458200T1 (de) * 2003-09-12 2010-03-15 Life Technologies Corp Anwendungen des resonanzenergietransfers zwischen terbium und gfp
US7570443B2 (en) 2003-09-19 2009-08-04 Applied Biosystems, Llc Optical camera alignment
US7417726B2 (en) * 2003-09-19 2008-08-26 Applied Biosystems Inc. Normalization of data using controls
US7431915B2 (en) 2003-10-31 2008-10-07 The Regents Of The University Of California Peptides whose uptake by cells is controllable
US20050214807A1 (en) * 2003-11-19 2005-09-29 Iain Johnson Environmental sensitive fluorogenic compounds and their application for singlet oxygen and protein detection
US7504495B2 (en) * 2003-11-20 2009-03-17 Exiqon A/S Quencher compositions comprising anthraquinone moieties
US7776529B2 (en) 2003-12-05 2010-08-17 Life Technologies Corporation Methine-substituted cyanine dye compounds
US7446202B2 (en) 2003-12-05 2008-11-04 Molecular Probes, Inc. Cyanine dye compounds
JP4127204B2 (ja) * 2003-12-17 2008-07-30 セイコーエプソン株式会社 液晶表示装置の製造方法
WO2005071096A2 (en) 2004-01-21 2005-08-04 Molecular Probes, Inc. Derivatives of cephalosporin and clavulanic acid for detecting beta-lacamase in a sample
US20050255485A1 (en) * 2004-05-14 2005-11-17 Livak Kenneth J Detection of gene duplications
EP2290073A3 (de) 2004-05-28 2011-08-31 Asuragen, Inc. Verfahren und Zusammensetzungen mit MicroRNA
EP1766089A1 (de) * 2004-06-30 2007-03-28 Applera Corporation Analogsondenkomplexe
US7732625B2 (en) * 2004-07-23 2010-06-08 Xerox Corporation Colorant compounds
US7311767B2 (en) * 2004-07-23 2007-12-25 Xerox Corporation Processes for preparing phase change inks
US7033424B2 (en) 2004-07-23 2006-04-25 Xerox Corporation Phase change inks
US20060024833A1 (en) 2004-07-27 2006-02-02 Molecular Probes, Inc. Fluorescent metal ion indicators with large stokes shift
CA2857878A1 (en) 2004-11-12 2006-12-28 Asuragen, Inc. Methods and compositions involving mirna and mirna inhibitor molecules
US20060292586A1 (en) * 2004-12-17 2006-12-28 Schroth Gary P ID-tag complexes, arrays, and methods of use thereof
US20060166238A1 (en) * 2004-12-22 2006-07-27 Ramsing Niels B Probes, libraries and kits for analysis of mixtures of nucleic acids and methods for constructing the same
US20060228726A1 (en) * 2005-01-06 2006-10-12 Martin Patrick K Polypeptides having nucleic acid binding activity and compositions and methods for nucleic acid amplification
EP1872135A2 (de) 2005-03-15 2008-01-02 Applera Corporation Verwendung von antikörper-surrogatantigen-systemen zum nachweis von analyten
EP1886145A2 (de) 2005-05-03 2008-02-13 Applera Corporation Fluoreszenznachweissystem und farbstoffsatz zur verwendung damit
JP5306811B2 (ja) 2005-05-11 2013-10-02 ライフ テクノロジーズ コーポレーション ２本鎖ｄｎａへの高い選択性を有する蛍光化学物質及びそれらの使用
AU2006259565B2 (en) 2005-06-15 2011-01-06 Complete Genomics, Inc. Single molecule arrays for genetic and chemical analysis
US20070087360A1 (en) * 2005-06-20 2007-04-19 Boyd Victoria L Methods and compositions for detecting nucleotides
WO2007030521A1 (en) * 2005-09-06 2007-03-15 Invitrogen Corporation Control of chemical modification
US20070059713A1 (en) 2005-09-09 2007-03-15 Lee Jun E SSB-DNA polymerase fusion proteins
WO2007133831A2 (en) 2006-02-24 2007-11-22 Callida Genomics, Inc. High throughput genome sequencing on dna arrays
EP1951900B1 (de) 2005-10-07 2016-06-15 Callida Genomics, Inc. Selbstzusammengesetzte einzelmolekül-arrays und verwendungen davon
US7521577B2 (en) 2006-01-12 2009-04-21 Molecular Probes, Inc. Heavy metal binding compounds and their method of use
US20070196860A1 (en) * 2006-01-18 2007-08-23 Invitrogen Corporation Methods for Measuring Real Time Kinase Activity
SG170028A1 (en) 2006-02-24 2011-04-29 Callida Genomics Inc High throughput genome sequencing on dna arrays
US9677123B2 (en) 2006-03-15 2017-06-13 Siemens Healthcare Diagnostics Inc. Degenerate nucleobase analogs
WO2007140015A2 (en) 2006-05-26 2007-12-06 Althea Technologies, Inc Biochemical analysis of partitioned cells
PT2062049E (pt) 2006-09-06 2014-07-28 Univ California Diagnóstico molecular e classificação de melanoma maligno
WO2008070352A2 (en) 2006-10-27 2008-06-12 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
JP5636189B2 (ja) 2006-10-27 2014-12-03 ライフ テクノロジーズ コーポレーション 蛍光発生性ｐＨ感受性色素およびその使用方法
US20090111706A1 (en) 2006-11-09 2009-04-30 Complete Genomics, Inc. Selection of dna adaptor orientation by amplification
US8586743B2 (en) * 2007-01-30 2013-11-19 Life Technologies Corporation Labeling reagents and methods of their use
US7485728B2 (en) * 2007-02-06 2009-02-03 Xerox Corporation Colorant compounds
US7485737B2 (en) * 2007-02-06 2009-02-03 Xerox Corporation Colorant compounds
US7736426B2 (en) 2007-02-06 2010-06-15 Xerox Corporation Phase change inks containing colorant compounds
US8163074B2 (en) 2007-02-06 2012-04-24 Xerox Corporation Phase change inks containing colorant compounds
US7910754B2 (en) 2007-02-06 2011-03-22 Xerox Corporation Colorant compounds
US20080187664A1 (en) 2007-02-06 2008-08-07 Xerox Corporation Phase change inks containing colorant compounds
EP2137523A1 (de) 2007-04-04 2009-12-30 Network Biosystems, Inc. Kunststoff-mikrofluid-trenn- und -nachweisplattformen
US20080274458A1 (en) * 2007-05-01 2008-11-06 Latham Gary J Nucleic acid quantitation methods
US8513451B2 (en) * 2007-05-30 2013-08-20 Hee Chol Kang Fluorescent phospholipase A2 indicators
US7816135B2 (en) 2007-07-05 2010-10-19 Becton, Dickinson And Company Method of analyzing lymphocytes
EP2198050A1 (de) 2007-09-14 2010-06-23 Asuragen, INC. Mikrornas mit unterschiedlicher expression bei zervikalkarzinom und verwendungen davon
WO2009067632A1 (en) * 2007-11-20 2009-05-28 Applied Biosystems Inc. Method of sequencing nucleic acids using elaborated nucleotide phosphorothiolate compounds
US8017338B2 (en) 2007-11-20 2011-09-13 Life Technologies Corporation Reversible di-nucleotide terminator sequencing
WO2009070805A2 (en) 2007-12-01 2009-06-04 Asuragen, Inc. Mir-124 regulated genes and pathways as targets for therapeutic intervention
AU2009221778A1 (en) 2008-03-05 2009-09-11 The Regents Of The University Of California Molecular diagnosis and classification of malignant melanoma
US8258111B2 (en) 2008-05-08 2012-09-04 The Johns Hopkins University Compositions and methods related to miRNA modulation of neovascularization or angiogenesis
US20100159452A1 (en) * 2008-12-22 2010-06-24 Roche Molecular Systems, Inc. Method For Detecting a Target Nucleic Acid in a Sample
WO2010099137A2 (en) 2009-02-26 2010-09-02 Osi Pharmaceuticals, Inc. In situ methods for monitoring the emt status of tumor cells in vivo
US20120142004A1 (en) 2009-05-21 2012-06-07 Siemens Healthcare Diagnostics Inc. Universal Tags With Non-Natural Nucleobases
WO2010135692A2 (en) 2009-05-22 2010-11-25 Asuragen, Inc. Mirna biomarkers of prostate disease
WO2010147654A2 (en) 2009-06-15 2010-12-23 Netbio Inc. Improved methods for forensic dna quantitation
CN102725632A (zh) 2009-08-28 2012-10-10 奥斯瑞根公司 肺病的miRNA生物标志物
JP5451556B2 (ja) * 2009-09-18 2014-03-26 キヤノン株式会社 色素化合物
US20130261003A1 (en) 2010-08-06 2013-10-03 Ariosa Diagnostics, In. Ligation-based detection of genetic variants
US20120034603A1 (en) 2010-08-06 2012-02-09 Tandem Diagnostics, Inc. Ligation-based detection of genetic variants
JP5494351B2 (ja) * 2010-08-24 2014-05-14 ソニー株式会社 蛍光強度補正方法、蛍光強度算出方法及び蛍光強度算出装置並びに蛍光強度補正プログラム
BR112013010585B1 (pt) 2010-10-29 2020-12-01 Asuragen, Inc. método de caracterização de um locus de fmr1 ou um locus de frm2 em uma amostra de dna e método de análise de um locus fmr1 ou um locus fmr2 de uma amostra de dna humana
CN107326073A (zh) 2010-12-29 2017-11-07 生命技术公司 作为荧光参考标准品的 ddao 化合物
US20120190020A1 (en) 2011-01-25 2012-07-26 Aria Diagnostics, Inc. Detection of genetic abnormalities
US10131947B2 (en) 2011-01-25 2018-11-20 Ariosa Diagnostics, Inc. Noninvasive detection of fetal aneuploidy in egg donor pregnancies
US20120219950A1 (en) 2011-02-28 2012-08-30 Arnold Oliphant Assay systems for detection of aneuploidy and sex determination
CN103917661A (zh) 2011-05-12 2014-07-09 网络百奥有限公司 用于快速多重扩增str基因座的方法和组合物
CN102337041A (zh) * 2011-07-19 2012-02-01 华东理工大学 一种制备罗丹明荧光染料的方法
AU2012309824A1 (en) 2011-09-12 2013-04-18 Abbvie Biotherapeutics Inc. Artificial NK cells and uses thereof
WO2013040251A2 (en) 2011-09-13 2013-03-21 Asurgen, Inc. Methods and compositions involving mir-135b for distinguishing pancreatic cancer from benign pancreatic disease
EP3604555B1 (de) 2011-10-14 2024-12-25 President and Fellows of Harvard College Sequenzierung durch strukturanordnung
CN102516992A (zh) * 2011-12-07 2012-06-27 天津工业大学 一种检测Hg2+离子的荧光探针及其合成方法和用途
US9968901B2 (en) 2012-05-21 2018-05-15 The Scripps Research Institute Methods of sample preparation
US9914967B2 (en) 2012-06-05 2018-03-13 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using DNA origami probes
US9952200B2 (en) 2012-07-20 2018-04-24 President And Fellows Of Harvard College Cell based quality control bioassays for nutriceutical and medicinal products
US9476089B2 (en) 2012-10-18 2016-10-25 President And Fellows Of Harvard College Methods of making oligonucleotide probes
WO2014093291A1 (en) 2012-12-10 2014-06-19 Advandx, Inc. Use of probes for mass spectrometric identification of microorganisms or cells and associated conditions of interest
CN105102484B (zh) 2013-01-30 2020-03-10 艾维拉斯生物科学公司 选择性递送分子及使用方法
US9540685B2 (en) 2013-03-15 2017-01-10 President And Fellows Of Harvard College Methods of identifying homologous genes using FISH
US10071990B2 (en) 2013-06-03 2018-09-11 The Research Foundation For The State University Of New York Site-specific orthogonal labeling of the carboxy terminus of α-tubulin in live cells
EP3760638A3 (de) 2013-09-26 2021-05-26 National University of Singapore Zusammensetzungen und verfahren unter verwendung von lysophosphatidylcholingerüsten
JP6339787B2 (ja) * 2013-10-09 2018-06-06 株式会社日立ハイテクノロジーズ 核酸の分析方法
CN122038564A (zh) 2014-01-05 2026-05-15 G·索兹 使用mirna比率确定肺癌
US10619210B2 (en) 2014-02-07 2020-04-14 The Johns Hopkins University Predicting response to epigenetic drug therapy
ES2832748T3 (es) 2014-08-19 2021-06-11 Hoffmann La Roche Procedimientos y composiciones para la detección de ácidos nucleicos
US20180320226A1 (en) 2014-08-19 2018-11-08 President And Fellows Of Harvard College RNA-Guided Systems For Probing And Mapping Of Nucleic Acids
AU2015315103B2 (en) 2014-09-09 2022-01-27 Igenomx International Genomics Corporation Methods and compositions for rapid nucleic acid library preparation
US10385380B2 (en) 2014-10-02 2019-08-20 The Regents Of The University Of California Personalized protease assay to measure protease activity in neoplasms
US20160194701A1 (en) 2014-11-21 2016-07-07 Nanostring Technologies, Inc. Enzyme- and amplification-free sequencing
WO2016094330A2 (en) 2014-12-08 2016-06-16 20/20 Genesystems, Inc Methods and machine learning systems for predicting the liklihood or risk of having cancer
WO2016111915A1 (en) 2015-01-06 2016-07-14 De Haas Anthony H Near-infrared fluorescent surgical dye markers
EP3247797B1 (de) 2015-01-12 2020-04-29 Life Technologies Corporation Verfahren, systeme und zusammensetzungen daraus zur kontrolle und quantifizierung der nukleinsäurebibliotheksqualität
EP3292214A1 (de) 2015-05-04 2018-03-14 Academisch Medisch Centrum Verfahren zur quantifizierung von mirnas mittels normalisierung
WO2017015543A1 (en) 2015-07-23 2017-01-26 Asuragen, Inc. Methods, compositions, kits, and uses for analysis of nucleic acids comprising repeating a/t-rich segments
US20170327876A1 (en) 2016-05-16 2017-11-16 Nanostring Technologies, Inc. Methods for detecting target nucleic acids in a sample
WO2018045162A1 (en) 2016-09-01 2018-03-08 Biogen Ma Inc. Biomarkers predictive of primary progressive multiple sclerosis and uses thereof
KR102476709B1 (ko) 2016-11-21 2022-12-09 나노스트링 테크놀로지스, 인크. 화학적 조성물 및 이것을 사용하는 방법
US11441174B2 (en) 2017-06-02 2022-09-13 Affymetrix, Inc. Array-based methods for analysing mixed samples using differently labelled allele-specific probes
CN110914448A (zh) 2017-06-02 2020-03-24 昂飞股份有限公司 使用差异性标记的等位基因特异性探针分析混合样品的基于阵列的方法
ES2998689T3 (en) 2017-08-02 2025-02-21 Univ California Optimized peptides for targeting human nerves and their use in image guided surgery, diagnostics and therapeutic delivery
KR102133794B1 (ko) * 2017-08-24 2020-07-15 주식회사 셀투인 세포 소기관 내 글루타치온 측정용 실시간 형광 이미징 센서 및 이의 제조 방법
WO2019104070A1 (en) 2017-11-21 2019-05-31 Nanostring Technologies, Inc. O-nitrobenzyl photocleavable bifunctional linker
JP7525402B2 (ja) 2018-05-14 2024-07-30 ナノストリング テクノロジーズ，インコーポレイティド 化学的組成物とそれを利用する方法
MX2021007492A (es) 2018-12-20 2021-10-13 Life Technologies Corp Colorante de rodamina asimétrica y uso de este en ensayos biológicos.
CA3124333A1 (en) 2018-12-20 2020-06-25 Life Technologies Corporation Modified rhodamine dye and use thereof in biological assays
EP3719144A1 (de) 2019-04-05 2020-10-07 Fundación para la Investigación Biomédica del Hospital Universitario de la Paz (FIBHULP) Mir-151a-3p als universelle endogene steuerung zur exosomenladungsnormalisierung
US20220267857A1 (en) 2019-07-19 2022-08-25 Fundación Para La Investigación Biomédikca Del Hospital Universitario La Paz (Fibhulp) Method for determining the response to treatment of a patient affected by non-small cell lung carcinoma (nsclc)
WO2021072289A1 (en) 2019-10-10 2021-04-15 Alume Biosciences, Inc. Methods and systems using peptides for targeting and mapping human nerves in image guided surgery, diagnostics and therapeutic delivery
US20230109070A1 (en) 2019-10-18 2023-04-06 The Board of Trustees og the Leland Standford Junior University Clinical- and industrial-scale intact-tissue sequencing
US11359238B2 (en) 2020-03-06 2022-06-14 Singular Genomics Systems, Inc. Linked paired strand sequencing
US12209273B2 (en) 2020-06-12 2025-01-28 10X Genomics, Inc. Nucleic acid assays using click chemistry bioconjugation
EP4153606A4 (de) 2020-07-13 2024-10-02 Singular Genomics Systems, Inc. Verfahren zur sequenzierung komplementärer polynukleotide
US20230304932A1 (en) 2020-07-23 2023-09-28 Life Technologies Corporation Compositions, systems and methods for biological analysis involving energy transfer dye conjugates and analytes comprising the same
CA3186955A1 (en) 2020-07-23 2022-01-27 Scott Benson Energy transfer dye conjugates for use in biological assays
JP7844447B2 (ja) 2020-09-16 2026-04-13 ブルカー スペーシャル バイオロジー，インコーポレイティド 化学組成物およびその使用方法
WO2022087485A1 (en) 2020-10-22 2022-04-28 Singular Genomics Systems, Inc. Nucleic acid circularization and amplification on a surface
US12071667B2 (en) 2020-11-04 2024-08-27 10X Genomics, Inc. Sequence analysis using meta-stable nucleic acid molecules
JP7721399B2 (ja) 2020-11-26 2025-08-12 キヤノン株式会社 新規化合物、及び硫化水素検出用の構造体
US20240207421A1 (en) 2020-12-04 2024-06-27 Alume Biosciences, Inc. Nerve targeting peptides and methods of use
KR20230137930A (ko) 2021-01-14 2023-10-05 알룸 바이오사이언시스 인코포레이티드 수술 절차에서 요관을 가시화하기 위한 방법 및 조성물
US12060603B2 (en) 2021-01-19 2024-08-13 10X Genomics, Inc. Methods for internally controlled in situ assays using padlock probes
US11486001B2 (en) 2021-02-08 2022-11-01 Singular Genomics Systems, Inc. Methods and compositions for sequencing complementary polynucleotides
US11884977B2 (en) 2021-03-12 2024-01-30 Singular Genomics Systems, Inc. Nanoarrays and methods of use thereof
EP4263868A4 (de) 2021-03-12 2024-11-27 Singular Genomics Systems, Inc. Nanoarrays und verfahren zur verwendung davon
US11578320B2 (en) 2021-04-27 2023-02-14 Singular Genomics Systems, Inc. High density sequencing and multiplexed priming
WO2022235764A1 (en) 2021-05-05 2022-11-10 Singular Genomics Systems, Inc. Multiomic analysis device and methods of use thereof
EP4347877A1 (de) 2021-06-01 2024-04-10 10X Genomics, Inc. Verfahren und zusammensetzungen zum nachweis von analyten und zur sondenauflösung
EP4347880B1 (de) 2021-06-02 2026-02-04 10X Genomics, Inc. Probenanalyse mit asymmetrischen zirkularisierbaren sonden
US20230026886A1 (en) 2021-07-13 2023-01-26 10X Genomics, Inc. Methods for preparing polymerized matrix with controllable thickness
WO2023004400A1 (en) 2021-07-21 2023-01-26 Life Technologies Corporation Dibenzoxanthene quenchers, uses, and methods of preparation
CA3224093A1 (en) 2021-07-30 2023-02-02 Jorge Ivan Hernandez Neuta Methods and compositions for synchronizing reactions in situ
US12139751B2 (en) 2021-07-30 2024-11-12 10X Genomics, Inc. Circularizable probes for in situ analysis
WO2023015192A1 (en) 2021-08-03 2023-02-09 10X Genomics, Inc. Nucleic acid concatemers and methods for stabilizing and/or compacting the same
US12529096B2 (en) 2021-08-03 2026-01-20 10X Genomics, Inc. Stabilization and/or compaction of nucleic acid structures
US12391984B2 (en) 2021-08-03 2025-08-19 10X Genomics, Inc. Compositions and methods for rolling circle amplification
US12460251B2 (en) 2021-08-03 2025-11-04 10X Genomics, Inc. Stabilization and/or compaction of nucleic acid molecules
WO2023023484A1 (en) 2021-08-16 2023-02-23 10X Genomics, Inc. Probes comprising a split barcode region and methods of use
WO2023023638A1 (en) 2021-08-20 2023-02-23 Singular Genomics Systems, Inc. Chemical and thermal assisted nucleic acid amplification methods
EP4423664A4 (de) 2021-10-25 2025-08-27 Singular Genomics Systems Inc Manipulation und detektion biologischer proben
EP4422792A4 (de) 2021-10-26 2025-12-17 Singular Genomics Systems Inc Gezielte multiplex-amplifikation von polynukleotiden
US20230242974A1 (en) 2021-12-27 2023-08-03 10X Genomics, Inc. Methods and compositions for rolling circle amplification
WO2023141588A1 (en) 2022-01-21 2023-07-27 10X Genomics, Inc. Multiple readout signals for analyzing a sample
AU2023223467A1 (en) 2022-02-23 2024-08-08 Insitro, Inc. Pooled optical screening and transcriptional measurements of cells comprising barcoded genetic perturbations
US11795505B2 (en) 2022-03-10 2023-10-24 Singular Genomics Systems, Inc. Nucleic acid delivery scaffolds
AU2023243205A1 (en) 2022-04-01 2024-09-19 10X Genomics, Inc. Compositions and methods for targeted masking of autofluorescence
US12559791B2 (en) 2022-05-06 2026-02-24 10X Genomics, Inc. Methods and compositions for in situ analysis of V(D)J sequences
EP4519674A1 (de) 2022-05-06 2025-03-12 10X Genomics, Inc. Analyse von antigen- und antigenrezeptorinteraktionen
WO2023220300A1 (en) 2022-05-11 2023-11-16 10X Genomics, Inc. Compositions and methods for in situ sequencing
EP4540607A1 (de) 2022-06-17 2025-04-23 10X Genomics, Inc. Katalytische entvernetzung von proben zur in-situ-analyse
WO2024036304A1 (en) 2022-08-12 2024-02-15 10X Genomics, Inc. Puma1 polymerases and uses thereof
US12116626B2 (en) 2022-08-16 2024-10-15 10X Genomics, Inc. AP50 polymerases and uses thereof
CN120129825A (zh) 2022-10-14 2025-06-10 10X基因组学有限公司 用于评估生物样品质量的方法、组合物和系统
WO2024102736A1 (en) 2022-11-08 2024-05-16 10X Genomics, Inc. Immobilization methods and compositions for in situ detection
EP4573210B1 (de) 2022-11-16 2026-05-06 10X Genomics, Inc. Verfahren zur analyse der genexprimierung in zellen in einem pellet
US20240218437A1 (en) 2022-12-16 2024-07-04 10X Genomics, Inc. Methods and compositions for assessing performance
EP4646492A1 (de) 2023-01-06 2025-11-12 10X Genomics, Inc. Verfahren und zusammensetzungen zur in-situ-analyse von variantensequenzen
WO2024163948A2 (en) 2023-02-03 2024-08-08 10X Genomics, Inc. In situ analysis of variant sequences in biological samples
WO2024196764A2 (en) 2023-03-17 2024-09-26 Nanostring Technologies, Inc. Assay for recombinase accessible chromatin and related compositions and methods
WO2025029831A1 (en) 2023-07-31 2025-02-06 10X Genomics, Inc. Methods and systems for targeted rna cleavage and target rna-primed rolling circle amplification
WO2025059422A1 (en) 2023-09-14 2025-03-20 Bruker Spatial Biology, Inc. Methods for detecting target nucleic acids in a sample
US20250207203A1 (en) 2023-12-20 2025-06-26 10X Genomics, Inc. In situ detection of copy number variations in biological samples
WO2025147587A1 (en) 2024-01-05 2025-07-10 Glyphic Biotechnologies, Inc. Protein sequencing using super-resolution imaging
WO2025189030A1 (en) 2024-03-07 2025-09-12 Stellaromics, Inc. Methods and systems for volumetric imaging
WO2025240918A1 (en) 2024-05-17 2025-11-20 10X Genomics, Inc. Systems and methods for generating codebooks
WO2025264702A1 (en) 2024-06-18 2025-12-26 10X Genomics, Inc. 3d hydrogels for nucleic acid sequencing
WO2026030369A1 (en) 2024-07-31 2026-02-05 10X Genomics, Inc. Methods and compositions for in situ analyte detection
WO2026055487A1 (en) 2024-09-06 2026-03-12 10X Genomics, Inc. Modified nucleotides with oligomer spacers
WO2026097020A1 (en) 2024-11-04 2026-05-07 10X Genomics, Inc. Polymerase for sequencing by synthesis

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE47451C (de) * Badische Anilin- und Sodafabrik in Ludwigshafen a. Rh Neuerung in dem Verfahren zur Darstellung von Farbstoffen aus der Gruppe des Metaamidophenolpbtalelns. (4
DE108347C (de) *
DE45263C (de) * Badische Anilin- und Sodafabrik in Ludwigshafen a. Rh Neuerung in dem Verfahren zur Darstellung von Farbstoffen aus der Gruppe des m - Amidophenol - Phtalei'ns
JPS488725B1 (de) * 1969-10-08 1973-03-17
FR2065117A5 (de) * 1969-10-08 1971-07-23 Fuji Photo Film Co Ltd
US4318846A (en) * 1979-09-07 1982-03-09 Syva Company Novel ether substituted fluorescein polyamino acid compounds as fluorescers and quenchers
US4622400A (en) * 1983-12-29 1986-11-11 The United States Of America As Represented By The United States Department Of Energy Preparation of certain m-aminophenols and the use thereof for preparation of laser dyes
DE3425631A1 (de) * 1984-07-12 1986-01-16 Basf Ag, 6700 Ludwigshafen Rhodaminfarbstoffe
US4855225A (en) * 1986-02-07 1989-08-08 Applied Biosystems, Inc. Method of detecting electrophoretically separated oligonucleotides
CA1340806C (en) * 1986-07-02 1999-11-02 James Merrill Prober Method, system and reagents for dna sequencing
US4900686A (en) * 1987-04-02 1990-02-13 Polaroid Corporation Fluorescent conjugates and biological diagnostic assay
EP0333649B1 (de) * 1988-03-01 1994-06-08 Ciba-Geigy Ag 3-Basisch substituierte 2-Methylfluorane
WO1991003476A1 (en) * 1989-09-08 1991-03-21 Applied Biosystems, Inc. 5- and 6-succinimidylcarboxylate isomers of rhodamine dyes
US5366860A (en) * 1989-09-29 1994-11-22 Applied Biosystems, Inc. Spectrally resolvable rhodamine dyes for nucleic acid sequence determination
US5188934A (en) * 1989-11-14 1993-02-23 Applied Biosystems, Inc. 4,7-dichlorofluorescein dyes as molecular probes
US5750409A (en) * 1991-11-18 1998-05-12 Boehringer Mannheim Gmbh Pentacyclic compounds and their use as absorption or fluorescent dyes
US5256799A (en) * 1992-07-14 1993-10-26 The United States Of Americas As Represented By The United States Department Of Energy Preparation of 6-hydroxyindolines and their use for preparation of novel laser dyes
EP0658167B1 (de) * 1992-09-03 1999-04-14 The Perkin-Elmer Corporation 4,7-dichlorofluoroszein-farbestoffe als molekulare sonden
US5283336A (en) * 1992-09-08 1994-02-01 The United States Of America As Represented By The United States Department Of Energy Method for preparation of 7-hydroxy-1,2,3,4-tetrahydroquinoline from 1,2,3,4-tetrahydroquinoline
JPH08503604A (ja) * 1992-09-18 1996-04-23 アモコ・コーポレーション プローブで用いるための緑色蛍光標識ヌクレオチド
US6020481A (en) * 1996-04-01 2000-02-01 The Perkin-Elmer Corporation Asymmetric benzoxanthene dyes
US5800996A (en) * 1996-05-03 1998-09-01 The Perkin Elmer Corporation Energy transfer dyes with enchanced fluorescence
US5847162A (en) * 1996-06-27 1998-12-08 The Perkin Elmer Corporation 4, 7-Dichlororhodamine dyes

Also Published As

Publication number Publication date
US5847162A (en) 1998-12-08
JP4961410B2 (ja) 2012-06-27
JP2000505503A (ja) 2000-05-09
CA2258243A1 (en) 1997-12-31
EP0915935A1 (de) 1999-05-19
JP2004305217A (ja) 2004-11-04
DE69704205D1 (de) 2001-04-12
WO1997049769A1 (en) 1997-12-31
US6025505A (en) 2000-02-15
AU709209B2 (en) 1999-08-26
JP2003221515A (ja) 2003-08-08
AU3285597A (en) 1998-01-14
JP4140976B2 (ja) 2008-08-27
DE69704205T2 (de) 2001-10-25
EP0915935B1 (de) 2001-03-07
JP2009073838A (ja) 2009-04-09
CA2258243C (en) 2007-11-13

Similar Documents

Publication Publication Date Title
DE69704205D1 (de) 4,7-dichlorrhodaminfarbstoffe
CA2367868A1 (en) 4,7-dichlororhodamine dyes useful as molecular probes
CA2304645A1 (en) Aromatic-substituted xanthene dyes
DE69700303D1 (de) Asymmetrische benzoxanthenfarbstoffe
BG102577A (bg) Нови 5-амино-3-циано-4-етилсулфинил-1-фенилпиразоли, метод за получаване и използването им като пестициди
GT199800197A (es) Proceso para la sintesis de 1,3-dioles.
ATE349514T1 (de) Chemisch modifizierte enzyme
DE69704055D1 (de) Kettenabbrechende nukleinsäure-sequenziermethode mit 2'-desoxyuridin-5'-triphosphat
DE69307131D1 (de) Selektive n-acylation von aminoalkoholen
DE59809639D1 (de) Verwendung von Carotinoid-Aggregaten als Färbemittel
BR9807857A (pt) Derivados de 1,4-diazacicloheptano e seu emprego em tinturas capilares oxidantes
EP1007626A4 (de) Nuklein- und aminosäurensequenzen einer neuen transketolase von mentha piperita
GB0326464D0 (en) Quinacridone labelling reagents for fluorescence detection of biological materials
ID21450A (id) S-oksida dan s,s-dioksida tetrahidrotiopiran pheniloksazolidinon
DE50012617D1 (de) Verwendung von methylchinolinium-verbindungen zum färben von keratinhaltigen fasern
ATE193013T1 (de) Zirkonverbindungen von sulfonsäuren
ATE212028T1 (de) Herstellung von tertiär phophinen durch nickel- katalysierte cross coupling
ES2122149T3 (es) Procedimiento para la preparacion de 1-cloro-1-fluoroetano y/o 1,1-difluoroetano.
DE59900379D1 (de) Verwendung von detergensgemischen als lösungsvermittler
ES2188273T3 (es) Farmacos anticarcinogenos a base de antraquinona.
ATE304001T1 (de) Verfahren zur herstellung von 2-alkyl-3-aryl- und heteroaryloxaziridinen und neue 2-alkyl-3- aryloxaziridine
BR0311200A (pt) Artigo para uso em um processo enzimático de limpeza de tecido, kit de partes e método para limpeza de tecido
BR0213191A (pt) Processo para reciclagem de gás de processo em processos eletroquìmicos
BR9904902A (pt) Agente de tingimento e processo para a produção de tingimentos de cabelo temporários
ATE209636T1 (de) Verbessertes verfahren zur herstellung von unsymmetrischen 4,6- bis(aryloxy)pyrimidinverbindungen

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties