ATE210725T1 - Verfahren zum einfangen und selektiven freisetzen von nukleinsäure - Google Patents

Verfahren zum einfangen und selektiven freisetzen von nukleinsäure

Info

Publication number
ATE210725T1
ATE210725T1 AT95306363T AT95306363T ATE210725T1 AT E210725 T1 ATE210725 T1 AT E210725T1 AT 95306363 T AT95306363 T AT 95306363T AT 95306363 T AT95306363 T AT 95306363T AT E210725 T1 ATE210725 T1 AT E210725T1
Authority
AT
Austria
Prior art keywords
nucleic acids
capture
nucleic acid
selective release
precipitate
Prior art date
Application number
AT95306363T
Other languages
English (en)
Inventor
Tobias E Ekeze
John Wesley Backus
David John Sharkey
Richard Calvin Sutton
Joanne Hansen Kerschner
Original Assignee
Johnson & Johnson Clin Diag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Johnson & Johnson Clin Diag filed Critical Johnson & Johnson Clin Diag
Application granted granted Critical
Publication of ATE210725T1 publication Critical patent/ATE210725T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT95306363T 1994-09-13 1995-09-12 Verfahren zum einfangen und selektiven freisetzen von nukleinsäure ATE210725T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/305,769 US5622822A (en) 1994-09-13 1994-09-13 Methods for capture and selective release of nucleic acids using polyethyleneimine and an anionic phosphate ester surfactant and amplification of same

Publications (1)

Publication Number Publication Date
ATE210725T1 true ATE210725T1 (de) 2001-12-15

Family

ID=23182269

Family Applications (1)

Application Number Title Priority Date Filing Date
AT95306363T ATE210725T1 (de) 1994-09-13 1995-09-12 Verfahren zum einfangen und selektiven freisetzen von nukleinsäure

Country Status (6)

Country Link
US (1) US5622822A (de)
EP (1) EP0726312B1 (de)
JP (1) JP3727692B2 (de)
AT (1) ATE210725T1 (de)
CA (1) CA2157968C (de)
DE (1) DE69524560T2 (de)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6846968B1 (en) 1988-02-26 2005-01-25 Large Scale Biology Corporation Production of lysosomal enzymes in plants by transient expression
GB9425138D0 (en) * 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
BR9815569A (pt) * 1997-12-06 2001-10-09 Dna Res Instr Ltd Método para extração de biomoléculas de material biológico
US6914137B2 (en) * 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
EP1147226B1 (de) * 1999-01-27 2013-01-23 Folim G. Halaka Materialien und verfahren zur reinigung von polyelektrolyten
US7683035B1 (en) 1999-02-23 2010-03-23 Qiagen, Gmbh Method of stabilizing and/or isolating nucleic acids
WO2000066783A2 (en) * 1999-05-04 2000-11-09 Ortho-Clinical Diagnostics, Inc. Rapid and efficient capture of dna from sample without using cell lysing reagent
US6617108B1 (en) 1999-07-12 2003-09-09 Technology Licensing Co. Llc Methods and compositions for biotechnical separations using selective precipitation by compaction agents
US6458526B1 (en) 2000-01-28 2002-10-01 Agilent Technologies, Inc. Method and apparatus to inhibit bubble formation in a fluid
US6406851B1 (en) * 2000-01-28 2002-06-18 Agilent Technologies, Inc. Method for coating a substrate quickly and uniformly with a small volume of fluid
US6730306B1 (en) 2000-03-08 2004-05-04 Large Scale Biology Corporation Parvovirus vaccine as viral coat protein fusions
CN100497655C (zh) 2000-05-01 2009-06-10 荣研化学株式会社 检测核酸合成的反应产物的方法
US7262006B1 (en) 2000-05-01 2007-08-28 Ortho-Clinical Diagnostics, Inc. Rapid and efficient capture of DNA from sample without using cell lysing reagent
US6806051B2 (en) * 2000-09-25 2004-10-19 Picoliter Inc. Arrays of partially nonhybridizing oligonucleotides and preparation thereof using focused acoustic energy
US20040106109A1 (en) * 2001-10-02 2004-06-03 Belly Robert T Detection of ras mutations
DE60216827T2 (de) * 2001-10-09 2007-06-28 Sysmex Corp. Verfahren und Vorrichtung zur Bestimmung von amplifizierten Genprodukten zum Nachweis eines Gens
US6927029B2 (en) 2001-12-03 2005-08-09 Agilent Technologies, Inc. Surface with tethered polymeric species for binding biomolecules
CN1617938A (zh) 2002-01-16 2005-05-18 戴诺生物技术有限公司 从单个样品中分离核酸和蛋白质的方法
US8394944B2 (en) * 2002-09-20 2013-03-12 Siemens Healthcare Diagnostics Inc. Dual-purpose primers and probes for providing enhanced hybridization assays by disruption of secondary structure formation
JP4437218B2 (ja) * 2002-11-26 2010-03-24 日生バイオ株式会社 Ｒｎａ−プロタミン複合体、その製造方法およびそれを含む健康食品
EP1802395B1 (de) * 2004-09-09 2020-01-22 Institut Curie Ein kollineares elektrisches feld verwendende mikrofluidvorrichtung
US8288169B2 (en) * 2005-01-21 2012-10-16 Argylla Technologies Surface mediated self-assembly of nanoparticles
US20090208919A1 (en) * 2005-01-21 2009-08-20 Argylla Technologies, Llp Particle matrix for storage of biomolecules
US7964380B2 (en) * 2005-01-21 2011-06-21 Argylia Technologies Nanoparticles for manipulation of biopolymers and methods of thereof
EP2152864A1 (de) * 2007-06-13 2010-02-17 Amersham Biosciences Corp. Polymerasestabilisierung
EP2513335A4 (de) * 2009-12-14 2013-09-11 Betty Wu Verfahren und materialien zur abscheidung von nukleinsäurematerialien
JP6153866B2 (ja) 2010-05-25 2017-06-28 キアゲン ガイサーズバーグ アイエヌシー． 迅速なハイブリッド捕捉アッセイ、及び関連する戦略的に切断されたプローブ
CN113355321A (zh) 2011-09-26 2021-09-07 凯杰有限公司 用于分离细胞外核酸的快速方法
PL2854981T3 (pl) * 2012-05-24 2018-11-30 Meridian Bioscience, Inc. Sposoby frakcjonowania i wykrywania kwasu nukleinowego
WO2014072367A1 (en) 2012-11-07 2014-05-15 Qiagen Gmbh Control for diagnostic assay
JP6787916B2 (ja) 2015-06-10 2020-11-18 キアゲン ゲーエムベーハー アニオン交換粒子を使用する細胞外核酸を単離する方法
DE102017200332A1 (de) 2017-01-11 2018-07-12 Robert Bosch Gmbh Verfahren zum Extrahieren von DNS

Family Cites Families (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3176006A (en) * 1963-08-06 1965-03-30 Zahn Rudolf Karl Process for treating desoxyribo-nucleic acid
DE2001902C3 (de) * 1970-01-16 1978-10-12 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren zur Reinigung und Fraktionierung von gelösten aktiven Proteinen
IE38174B1 (en) * 1972-07-07 1978-01-18 Snam Progetti Process for purifying an enzyme
US4089747A (en) 1976-08-09 1978-05-16 Eastman Kodak Company Compositions for the detection of hydrogen peroxide
CA1180647A (en) 1981-07-17 1985-01-08 Cavit Akin Light-emitting polynucleotide hybridization diagnostic method
US4994373A (en) 1983-01-27 1991-02-19 Enzo Biochem, Inc. Method and structures employing chemically-labelled polynucleotide probes
US4713326A (en) 1983-07-05 1987-12-15 Molecular Diagnostics, Inc. Coupling of nucleic acids to solid support by photochemical methods
DE3401620A1 (de) * 1984-01-18 1985-07-18 Boehringer Mannheim Gmbh, 6800 Mannheim Restriktionsendonuklease mae iii, ihre gewinnung und verwendung
US4920061A (en) 1984-03-02 1990-04-24 The University Of Texas System Biological magnetic colloids
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4795698A (en) 1985-10-04 1989-01-03 Immunicon Corporation Magnetic-polymer particles
CA1339653C (en) 1986-02-25 1998-02-03 Larry J. Johnson Appartus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US4997772A (en) 1987-09-18 1991-03-05 Eastman Kodak Company Water-insoluble particle and immunoreactive reagent, analytical elements and methods of use
JPH0736015B2 (ja) 1987-09-18 1995-04-19 イーストマン コダック カンパニー 診断試験キットおよびリガンドの測定方法
US4962029A (en) 1987-10-02 1990-10-09 Cetus Corporation Covalent oligonucleotide-horseradish peroxidase conjugate
US4914210A (en) 1987-10-02 1990-04-03 Cetus Corporation Oligonucleotide functionalizing reagents
US4902624A (en) 1987-11-23 1990-02-20 Eastman Kodak Company Temperature cycling cuvette
CA1323293C (en) 1987-12-11 1993-10-19 Keith C. Backman Assay using template-dependent nucleic acid probe reorganization
IL88923A (en) 1988-01-12 1995-07-31 Hoffmann La Roche Gene encoding a thermostable dna polymerase from thermus aquaticus said dna polymerase and its purification
EP0356395A3 (de) * 1988-08-26 1991-09-04 Ciba-Geigy Ag Flankierende Peptide und Verfahren zu ihrer Herstellung
US5229297A (en) 1989-02-03 1993-07-20 Eastman Kodak Company Containment cuvette for PCR and method of use
WO1990008840A1 (en) 1989-02-03 1990-08-09 Eastman Kodak Company Nucleic acid test article and its use to detect a predetermined nucleic acid
US5334499A (en) 1989-04-17 1994-08-02 Eastman Kodak Company Methods of extracting, amplifying and detecting a nucleic acid from whole blood or PBMC fraction
US5092992A (en) * 1989-06-07 1992-03-03 J. T. Baker Inc. Polyethyleneimine matrixes for affinity chromatography
US5141857A (en) * 1989-06-09 1992-08-25 Gene-Trak Systems Purification of q beta replicase
US5089233A (en) 1989-06-12 1992-02-18 Eastman Kodak Company Processing apparatus for a chemical reaction pack
US5231015A (en) 1989-10-18 1993-07-27 Eastman Kodak Company Methods of extracting nucleic acids and pcr amplification without using a proteolytic enzyme
CA2031659A1 (en) 1990-01-26 1991-07-27 John B. Findlay Water-insoluble reagent, nucleic acid probe, test kit and diagnostic and purification methods
CA2035010C (en) 1990-01-26 1996-12-10 Keith C. Backman Method of amplifying target nucleic acids applicable to both polymerase and ligase chain reactions
DK0515506T3 (da) 1990-02-16 2000-05-08 Hoffmann La Roche Fremgangsmåde til påvisning af carcinogene humane papillomavirus
US5155166A (en) 1990-06-18 1992-10-13 Eastman Kodak Company Use of 1-(1-pyrrolidinylcarbonyl)pyridinium salts to attach compounds to carboxylated particles and a kit containing same
US5147777A (en) 1990-06-18 1992-09-15 Eastman Kodak Company Biologically active reagents prepared from carboxy-containing polymer, analytical element and methods of use
US5173260A (en) 1990-09-17 1992-12-22 Eastman Kodak Company Beads fused to a test device support
DE69128520T2 (de) 1990-10-31 1998-07-09 Tosoh Corp Verfahren zum Nachweis oder Quantifizierung von Zielnukleinsäuren
JPH0830704B2 (ja) 1991-03-21 1996-03-27 イーストマン コダック カンパニー 核酸増幅のための要素および方法ならびに付着プローブを使用する検出方法
IL101356A (en) * 1991-04-03 1996-08-04 Perkin Elmer Corp Solvents for the separation of nucleic acids by replacing anions
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5338671A (en) 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
US5473060A (en) * 1993-07-02 1995-12-05 Lynx Therapeutics, Inc. Oligonucleotide clamps having diagnostic applications

Also Published As

Publication number Publication date
US5622822A (en) 1997-04-22
EP0726312A3 (de) 1998-01-14
CA2157968C (en) 2009-11-10
CA2157968A1 (en) 1996-03-14
DE69524560T2 (de) 2002-05-29
EP0726312A2 (de) 1996-08-14
JPH08173159A (ja) 1996-07-09
EP0726312B1 (de) 2001-12-12
JP3727692B2 (ja) 2005-12-14
DE69524560D1 (de) 2002-01-24

Similar Documents

Publication Publication Date Title
ATE210725T1 (de) Verfahren zum einfangen und selektiven freisetzen von nukleinsäure
SI0707077T1 (en) Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
ATE114334T1 (de) Chaotropisches verfahren zur schätzung von nukleinen säuren in einer biologischen probe.
ATE178098T1 (de) Vorrichtung zur dürchführung von magnetischen zyklusreactionen
ATE234155T1 (de) Verfahren und vorrichtung zur behandlung von nukleinsäuren aus einer probe
ATE141651T1 (de) Verfahren zum nachweis und zur identifizierung von nukleinsäure enthaltenden teilen
DE69031957D1 (de) Verfahren zur Extraktion, Amplifikation und zum Nachweis von Nukleinsäuren aus Vollblut
AU6697401A (en) Detection of nucleic acids by type-specific hybrid capture method
ATE393137T1 (de) Verfahren zur herstellung von carbonsäuren und deren derivaten
IL81219A0 (en) Method and kit for detecting viruses by amplification and hybridization of nucleic acid sequences and compositions containing such amplified sequences
DE59000762D1 (de) Verfahren zur schnellen detektion von mikroorganismen in proben und vorrichtung zur durchfuehrung des verfahrens.
DE69316372D1 (de) Eliminierung von falschen negativen in nuklein-säuren
DK1206571T3 (da) Hurtig og effektiv indfangning af DNA fra pröve uden anvendelse af cellelyseringsreagens
DE69121821D1 (de) Nukleotid-Sequenzen nützlich als typenspezifische Sonden, PCR Primers und LCR Sonden zur Amplifikation und zum Nachweis von humanem Papillomavirus, sowie dazu verwendete Kits und Verfahren
EP0128018A3 (en) Molecular genetic probe, and method of forming same, assay technique and kit using said molecular genetic probe
ATE422212T1 (de) Verfahren zur reinigung von rekombinierten hexose oxidase
DE59107960D1 (de) Verfahren zur Bestimmung von Proteinen in Körperflüssigkeiten und Mittel zur Durchführung des Verfahrens
ATE123315T1 (de) Verfahren zur reinigung, vervielfachung und zum nachweis einer nukleinsäure.
ATE200036T1 (de) Verfahren zur behandlung von molekülen und vorrichtung dafür
DE69331443D1 (de) Verfahren zur bestimmung von nukleinsäuren
Schiff et al. A safranin-fast green stain for the differentiation of the nuclei of Rumen Protozoa
DE60238199D1 (de) Automatisches verfahren zum nachweis pathogener organismen in wasser
Chen Determination of Carbon in the Neodymium
ATA411879A (de) Verfahren zum rueckgewinnen von magnefite-koch- saeure und vorrichtung zur durchfuehrung des verfahrens
ATE58361T1 (de) Verfahren zur einbringung von sauerstoff in wasser und vorrichtung zur durchfuehrung des verfahrens.

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties