ATE212719T1 - Einschliessen von matrizen zur sequenzierung von nukleinsaeuren in probenmischungen bei verflochtenen polymernetzwerken - Google Patents
Einschliessen von matrizen zur sequenzierung von nukleinsaeuren in probenmischungen bei verflochtenen polymernetzwerkenInfo
- Publication number
- ATE212719T1 ATE212719T1 AT95904142T AT95904142T ATE212719T1 AT E212719 T1 ATE212719 T1 AT E212719T1 AT 95904142 T AT95904142 T AT 95904142T AT 95904142 T AT95904142 T AT 95904142T AT E212719 T1 ATE212719 T1 AT E212719T1
- Authority
- AT
- Austria
- Prior art keywords
- matrix
- nucleic acids
- polymer networks
- sequencing nucleic
- sample mixtures
- Prior art date
Links
- 229920000642 polymer Polymers 0.000 title abstract 4
- 239000000203 mixture Substances 0.000 title 1
- 150000007523 nucleic acids Chemical class 0.000 title 1
- 102000039446 nucleic acids Human genes 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 title 1
- 238000012163 sequencing technique Methods 0.000 title 1
- 239000011159 matrix material Substances 0.000 abstract 5
- 238000005251 capillar electrophoresis Methods 0.000 abstract 2
- 238000001712 DNA sequencing Methods 0.000 abstract 1
- 230000005684 electric field Effects 0.000 abstract 1
- 229920002521 macromolecule Polymers 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44743—Introducing samples
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44747—Composition of gel or of carrier mixture
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Organic Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Physics & Mathematics (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Pathology (AREA)
- Electrochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Dispersion Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US15621893A | 1993-11-23 | 1993-11-23 | |
| PCT/US1994/013605 WO1995014922A1 (en) | 1993-11-23 | 1994-11-22 | Entrapment of nucleic acid sequencing template in sample mixtures by entangled polymer networks |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE212719T1 true ATE212719T1 (de) | 2002-02-15 |
Family
ID=22558621
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT95904142T ATE212719T1 (de) | 1993-11-23 | 1994-11-22 | Einschliessen von matrizen zur sequenzierung von nukleinsaeuren in probenmischungen bei verflochtenen polymernetzwerken |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP0680605B1 (de) |
| JP (1) | JP2712058B2 (de) |
| AT (1) | ATE212719T1 (de) |
| AU (2) | AU8082794A (de) |
| CA (1) | CA2153175C (de) |
| DE (1) | DE69429765T2 (de) |
| WO (2) | WO1995014921A1 (de) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5891313A (en) * | 1993-11-23 | 1999-04-06 | The Perkin-Elmer Corp. | Entrapment of nucleic acid sequencing template in sample mixtures by entangled polymer networks |
| US6146511A (en) * | 1998-01-30 | 2000-11-14 | The Perkin-Elmer Corporation | Electrophoretic nucleic acid purification method |
| ATE372514T1 (de) | 2000-11-02 | 2007-09-15 | Gene Bio Applic Ltd | Gel für elektrophorese |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4737258A (en) * | 1984-05-14 | 1988-04-12 | Fuji Photo Film Co., Ltd. | Element for electrophoresis containing polyacrylamide gel membrane |
| US4911807A (en) * | 1989-09-05 | 1990-03-27 | Bio-Rad Laboratories, Inc. | Fractionation and sample loading by cassette in capillary electrophoresis |
| DE69104775T2 (de) * | 1990-05-29 | 1995-06-01 | Waters Investments Ltd | Verfahren und Vorrichtung zum Durchführen der Kapillarelektroforese. |
| US5085756A (en) * | 1990-08-21 | 1992-02-04 | Hewlett-Packard Company | Column separation system for electrophoresis with sample pretreatment |
| EP0497448A1 (de) * | 1991-02-01 | 1992-08-05 | Beckman Instruments, Inc. | Kapillar-Gel-Elektrophorese in Anwesenheit von mindestens einem von Harnstoff verschiedenen Denaturierungsreagens |
| US5340452A (en) * | 1991-02-01 | 1994-08-23 | Beckman Instruments, Inc. | On-column preconcentration of samples in capillary electrophoresis |
-
1994
- 1994-10-18 WO PCT/US1994/011889 patent/WO1995014921A1/en not_active Ceased
- 1994-10-18 AU AU80827/94A patent/AU8082794A/en not_active Abandoned
- 1994-11-22 AT AT95904142T patent/ATE212719T1/de not_active IP Right Cessation
- 1994-11-22 WO PCT/US1994/013605 patent/WO1995014922A1/en not_active Ceased
- 1994-11-22 JP JP7515253A patent/JP2712058B2/ja not_active Expired - Fee Related
- 1994-11-22 AU AU12946/95A patent/AU671569B2/en not_active Ceased
- 1994-11-22 CA CA002153175A patent/CA2153175C/en not_active Expired - Fee Related
- 1994-11-22 EP EP95904142A patent/EP0680605B1/de not_active Expired - Lifetime
- 1994-11-22 DE DE69429765T patent/DE69429765T2/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| WO1995014921A1 (en) | 1995-06-01 |
| EP0680605B1 (de) | 2002-01-30 |
| JP2712058B2 (ja) | 1998-02-10 |
| EP0680605A1 (de) | 1995-11-08 |
| JPH08506424A (ja) | 1996-07-09 |
| AU8082794A (en) | 1995-06-13 |
| CA2153175A1 (en) | 1995-06-01 |
| DE69429765D1 (de) | 2002-03-14 |
| WO1995014922A1 (en) | 1995-06-01 |
| CA2153175C (en) | 2002-10-15 |
| DE69429765T2 (de) | 2002-08-08 |
| AU671569B2 (en) | 1996-08-29 |
| AU1294695A (en) | 1995-06-13 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |