ATE212719T1 - Einschliessen von matrizen zur sequenzierung von nukleinsaeuren in probenmischungen bei verflochtenen polymernetzwerken - Google Patents

Einschliessen von matrizen zur sequenzierung von nukleinsaeuren in probenmischungen bei verflochtenen polymernetzwerken

Info

Publication number
ATE212719T1
ATE212719T1 AT95904142T AT95904142T ATE212719T1 AT E212719 T1 ATE212719 T1 AT E212719T1 AT 95904142 T AT95904142 T AT 95904142T AT 95904142 T AT95904142 T AT 95904142T AT E212719 T1 ATE212719 T1 AT E212719T1
Authority
AT
Austria
Prior art keywords
matrix
nucleic acids
polymer networks
sequencing nucleic
sample mixtures
Prior art date
Application number
AT95904142T
Other languages
English (en)
Inventor
Ben F Johnson
Original Assignee
Pe Corp Ny
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pe Corp Ny filed Critical Pe Corp Ny
Application granted granted Critical
Publication of ATE212719T1 publication Critical patent/ATE212719T1/de

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416Systems
    • G01N27/447Systems using electrophoresis
    • G01N27/44704Details; Accessories
    • G01N27/44743Introducing samples
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416Systems
    • G01N27/447Systems using electrophoresis
    • G01N27/44704Details; Accessories
    • G01N27/44747Composition of gel or of carrier mixture

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Electrochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT95904142T 1993-11-23 1994-11-22 Einschliessen von matrizen zur sequenzierung von nukleinsaeuren in probenmischungen bei verflochtenen polymernetzwerken ATE212719T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US15621893A 1993-11-23 1993-11-23
PCT/US1994/013605 WO1995014922A1 (en) 1993-11-23 1994-11-22 Entrapment of nucleic acid sequencing template in sample mixtures by entangled polymer networks

Publications (1)

Publication Number Publication Date
ATE212719T1 true ATE212719T1 (de) 2002-02-15

Family

ID=22558621

Family Applications (1)

Application Number Title Priority Date Filing Date
AT95904142T ATE212719T1 (de) 1993-11-23 1994-11-22 Einschliessen von matrizen zur sequenzierung von nukleinsaeuren in probenmischungen bei verflochtenen polymernetzwerken

Country Status (7)

Country Link
EP (1) EP0680605B1 (de)
JP (1) JP2712058B2 (de)
AT (1) ATE212719T1 (de)
AU (2) AU8082794A (de)
CA (1) CA2153175C (de)
DE (1) DE69429765T2 (de)
WO (2) WO1995014921A1 (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5891313A (en) * 1993-11-23 1999-04-06 The Perkin-Elmer Corp. Entrapment of nucleic acid sequencing template in sample mixtures by entangled polymer networks
US6146511A (en) * 1998-01-30 2000-11-14 The Perkin-Elmer Corporation Electrophoretic nucleic acid purification method
ATE372514T1 (de) 2000-11-02 2007-09-15 Gene Bio Applic Ltd Gel für elektrophorese

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4737258A (en) * 1984-05-14 1988-04-12 Fuji Photo Film Co., Ltd. Element for electrophoresis containing polyacrylamide gel membrane
US4911807A (en) * 1989-09-05 1990-03-27 Bio-Rad Laboratories, Inc. Fractionation and sample loading by cassette in capillary electrophoresis
DE69104775T2 (de) * 1990-05-29 1995-06-01 Waters Investments Ltd Verfahren und Vorrichtung zum Durchführen der Kapillarelektroforese.
US5085756A (en) * 1990-08-21 1992-02-04 Hewlett-Packard Company Column separation system for electrophoresis with sample pretreatment
EP0497448A1 (de) * 1991-02-01 1992-08-05 Beckman Instruments, Inc. Kapillar-Gel-Elektrophorese in Anwesenheit von mindestens einem von Harnstoff verschiedenen Denaturierungsreagens
US5340452A (en) * 1991-02-01 1994-08-23 Beckman Instruments, Inc. On-column preconcentration of samples in capillary electrophoresis

Also Published As

Publication number Publication date
WO1995014921A1 (en) 1995-06-01
EP0680605B1 (de) 2002-01-30
JP2712058B2 (ja) 1998-02-10
EP0680605A1 (de) 1995-11-08
JPH08506424A (ja) 1996-07-09
AU8082794A (en) 1995-06-13
CA2153175A1 (en) 1995-06-01
DE69429765D1 (de) 2002-03-14
WO1995014922A1 (en) 1995-06-01
CA2153175C (en) 2002-10-15
DE69429765T2 (de) 2002-08-08
AU671569B2 (en) 1996-08-29
AU1294695A (en) 1995-06-13

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ATE212719T1 (de) Einschliessen von matrizen zur sequenzierung von nukleinsaeuren in probenmischungen bei verflochtenen polymernetzwerken

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