ATE214741T1 - Verfahren zur amplifizierung von nukleinsäuse sequenzen durch verdrängung mit hilfe von chimären primer - Google Patents

Verfahren zur amplifizierung von nukleinsäuse sequenzen durch verdrängung mit hilfe von chimären primer

Info

Publication number
ATE214741T1
ATE214741T1 AT96926456T AT96926456T ATE214741T1 AT E214741 T1 ATE214741 T1 AT E214741T1 AT 96926456 T AT96926456 T AT 96926456T AT 96926456 T AT96926456 T AT 96926456T AT E214741 T1 ATE214741 T1 AT E214741T1
Authority
AT
Austria
Prior art keywords
target
pct
segment
dna
hybridizing
Prior art date
Application number
AT96926456T
Other languages
English (en)
Inventor
Philippe Cleuziat
Bernard Mandrand
Original Assignee
Bio Merieux
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bio Merieux filed Critical Bio Merieux
Application granted granted Critical
Publication of ATE214741T1 publication Critical patent/ATE214741T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT96926456T 1995-07-24 1996-07-24 Verfahren zur amplifizierung von nukleinsäuse sequenzen durch verdrängung mit hilfe von chimären primer ATE214741T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR9508945A FR2737223B1 (fr) 1995-07-24 1995-07-24 Procede d'amplification de sequences d'acide nucleique par deplacement, a l'aide d'amorces chimeres
PCT/FR1996/001166 WO1997004126A1 (fr) 1995-07-24 1996-07-24 Procede d'amplification de sequences d'acide nucleique par deplacement, a l'aide d'amorces chimeres

Publications (1)

Publication Number Publication Date
ATE214741T1 true ATE214741T1 (de) 2002-04-15

Family

ID=9481300

Family Applications (1)

Application Number Title Priority Date Filing Date
AT96926456T ATE214741T1 (de) 1995-07-24 1996-07-24 Verfahren zur amplifizierung von nukleinsäuse sequenzen durch verdrängung mit hilfe von chimären primer

Country Status (7)

Country Link
US (1) US5824517A (de)
EP (1) EP0787209B1 (de)
AT (1) ATE214741T1 (de)
CA (1) CA2200627C (de)
DE (1) DE69619959T2 (de)
FR (1) FR2737223B1 (de)
WO (1) WO1997004126A1 (de)

Families Citing this family (143)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050123926A1 (en) * 1994-01-13 2005-06-09 Enzo Diagnostics, Inc., In vitro process for producing multiple nucleic acid copies
US20110097791A1 (en) * 1999-04-16 2011-04-28 Engelhardt Dean L Novel process, construct and conjugate for producing multiple nucleic acid copies
US6001610A (en) * 1994-11-23 1999-12-14 Roche Diagnostics, Gmbh Method for the particularly sensitive detection of nucleic acids
US5916779A (en) * 1995-09-21 1999-06-29 Becton, Dickinson And Company Strand displacement amplification of RNA targets
US5853990A (en) 1996-07-26 1998-12-29 Edward E. Winger Real time homogeneous nucleotide assay
US7070921B2 (en) 2000-04-28 2006-07-04 Molecular Devices Corporation Molecular modification assays
US7745142B2 (en) 1997-09-15 2010-06-29 Molecular Devices Corporation Molecular modification assays
US7632651B2 (en) 1997-09-15 2009-12-15 Mds Analytical Technologies (Us) Inc. Molecular modification assays
US6312902B1 (en) 1998-03-13 2001-11-06 Promega Corporation Nucleic acid detection
US6268146B1 (en) 1998-03-13 2001-07-31 Promega Corporation Analytical methods and materials for nucleic acid detection
US6391551B1 (en) 1998-03-13 2002-05-21 Promega Corporation Detection of nucleic acid hybrids
US6277578B1 (en) 1998-03-13 2001-08-21 Promega Corporation Deploymerization method for nucleic acid detection of an amplified nucleic acid target
US6235480B1 (en) 1998-03-13 2001-05-22 Promega Corporation Detection of nucleic acid hybrids
US6270974B1 (en) 1998-03-13 2001-08-07 Promega Corporation Exogenous nucleic acid detection
US6270973B1 (en) 1998-03-13 2001-08-07 Promega Corporation Multiplex method for nucleic acid detection
US7090975B2 (en) 1998-03-13 2006-08-15 Promega Corporation Pyrophosphorolysis and incorporation of nucleotide method for nucleic acid detection
US6703211B1 (en) 1998-03-13 2004-03-09 Promega Corporation Cellular detection by providing high energy phosphate donor other than ADP to produce ATP
GB9806253D0 (en) * 1998-03-25 1998-05-20 Tepnel Medical Ltd Amplification of nucleic acids
JP4438110B2 (ja) * 1998-07-01 2010-03-24 東ソー株式会社 標的核酸の定量方法
DE19834948A1 (de) * 1998-08-03 2000-02-17 Monika Jecht Verfahren zur spezifischen Detektion von RNA
DE19856064C2 (de) * 1998-12-04 2000-11-30 Invitek Gmbh Universelles Verfahren zur Isolierung von DNA aus beliebigen Ausgangsmaterialien
CN100432237C (zh) * 1999-03-19 2008-11-12 宝生物工程株式会社 用于扩增核酸序列的方法
CN1227357C (zh) * 1999-03-19 2005-11-16 宝生物工程株式会社 用于扩增核酸序列的方法
US6951722B2 (en) 1999-03-19 2005-10-04 Takara Bio Inc. Method for amplifying nucleic acid sequence
US6232104B1 (en) * 1999-08-17 2001-05-15 Dade Behring Inc. Detection of differences in nucleic acids by inhibition of spontaneous DNA branch migration
US6677445B1 (en) 1999-08-27 2004-01-13 Chiron Corporation Chimeric antisense oligonucleotides and cell transfecting formulations thereof
US6251639B1 (en) 1999-09-13 2001-06-26 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences, using a RNA-DNA composite primer
US6692918B2 (en) * 1999-09-13 2004-02-17 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
DE60135912D1 (de) * 2000-05-12 2008-11-06 Caliper Life Sciences Inc Nachweis der hybridisierung von nukleinsäuren durch fluoreszenzpolarisation
JP2004513617A (ja) * 2000-06-26 2004-05-13 ニューゲン　テクノロジーズ，　インコーポレイテッド 転写に基づく核酸増幅のための方法および組成物
US7846733B2 (en) 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
KR100735131B1 (ko) * 2000-08-23 2007-07-03 다카라 바이오 가부시키가이샤 핵산 증폭 방법
EA004630B1 (ru) * 2000-09-19 2004-06-24 Такара Био Инк. Способ образования комплекса
JP2004537257A (ja) 2000-10-06 2004-12-16 ニューゲン テクノロジーズ， インコーポレイテッド 核酸配列の検出および／または定量のための、方法およびプローブ
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
AU2002228974A1 (en) 2000-12-13 2002-06-24 Nugen Technologies, Inc Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
EP1347060A4 (de) * 2000-12-26 2004-08-18 Takara Bio Inc Verfahren zum nachweis pathogener mikroorganismen
EP1359219A4 (de) * 2001-02-06 2004-09-22 Takara Bio Inc Amplifizierte nukleinsäuren und immobilisierte produkte davon
US6946251B2 (en) * 2001-03-09 2005-09-20 Nugen Technologies, Inc. Methods and compositions for amplification of RNA sequences using RNA-DNA composite primers
CA2439074A1 (en) * 2001-03-09 2002-09-19 Nugen Technologies, Inc. Methods and compositions for amplification of rna sequences
CN100379861C (zh) * 2001-06-12 2008-04-09 宝生物工程株式会社 核酸扩增或检测用试剂的稳定化方法和保存方法
US9777312B2 (en) * 2001-06-30 2017-10-03 Enzo Life Sciences, Inc. Dual polarity analysis of nucleic acids
US9261460B2 (en) 2002-03-12 2016-02-16 Enzo Life Sciences, Inc. Real-time nucleic acid detection processes and compositions
US20040161741A1 (en) 2001-06-30 2004-08-19 Elazar Rabani Novel compositions and processes for analyte detection, quantification and amplification
US7056671B2 (en) * 2001-08-20 2006-06-06 Takara Bio Inc. Isothermal chimeric primer nucleic acid amplification methods using blocking oglionucleotide
US7045319B2 (en) * 2001-10-30 2006-05-16 Ribomed Biotechnologies, Inc. Molecular detection systems utilizing reiterative oligonucleotide synthesis
US20040054162A1 (en) * 2001-10-30 2004-03-18 Hanna Michelle M. Molecular detection systems utilizing reiterative oligonucleotide synthesis
JP4210769B2 (ja) * 2001-11-22 2009-01-21 独立行政法人産業技術総合研究所 Ｇａｔｅｗａｙエントリークローンの作製方法
US7629152B2 (en) * 2002-03-01 2009-12-08 Integrated Dna Technologies, Inc. Methods for amplifying polymeric nucleic acids
EP1481088B1 (de) * 2002-03-01 2008-09-17 Integrated Dna Technologies, Inc. Polynomiale amplifikation von nukleinsäuren
US7176025B2 (en) * 2002-03-11 2007-02-13 Nugen Technologies, Inc. Methods for generating double stranded DNA comprising a 3′ single stranded portion and uses of these complexes for recombination
US9353405B2 (en) 2002-03-12 2016-05-31 Enzo Life Sciences, Inc. Optimized real time nucleic acid detection processes
US7393635B2 (en) * 2002-05-16 2008-07-01 Dna Polymerase Technology, Inc. Ribocloning: recombinant DNA construction using primers with ribo bases
EP1573056A4 (de) * 2002-05-17 2007-11-28 Nugen Technologies Inc Verfahren zur fragmentierung, markierung und immobilisierung von nukleinsäuren
TW200413527A (en) * 2002-10-29 2004-08-01 Riken Amplification of nucleic acid
EP1576188B1 (de) * 2002-11-21 2008-10-15 Epicentre Technologies Verfahren zur verwendung von riboprimern zur strangverdrängungsreplikation von zielsequenzen
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
US7955795B2 (en) * 2003-06-06 2011-06-07 Qiagen Gmbh Method of whole genome amplification with reduced artifact production
AU2003900368A0 (en) * 2003-01-24 2003-02-13 Human Genetic Signatures Pty Ltd Assay for nucleic acid molecules
US8043834B2 (en) 2003-03-31 2011-10-25 Qiagen Gmbh Universal reagents for rolling circle amplification and methods of use
WO2004092418A2 (en) * 2003-04-14 2004-10-28 Nugen Technologies, Inc. Global amplification using a randomly primed composite primer
US7288373B2 (en) * 2003-05-02 2007-10-30 Human Genetic Signatures Pty Ltd. Treatment of methylated nucleic acid
ATE476523T1 (de) * 2003-06-17 2010-08-15 Human Genetic Signatures Pty Verfahren zur genomamplifikation
RU2258740C2 (ru) * 2003-08-21 2005-08-20 Вафин Рамиль Ришадович СПОСОБ ДЕТЕКЦИИ МИКРООРГАНИЗМОВ С ПОМОЩЬЮ ИЗОТЕРМИЧЕСКОЙ РЕАКЦИИ АМПЛИФИКАЦИИ-RAMIL (РЕВЕРТАЗНОЕ АМПЛИФИКАЦИОННОЕ ЗАКЛЮЧЕНИЕ (REVERTASE AMPLIFICATION ILLATION) ИЛИ РНК-аза H ЗАВИСИМОЕ АМПЛИФИКАЦИОННОЕ ЗАКЛЮЧЕНИЕ (RNAase Н-DEPENDENT AMPLIFICATION ILLATION)
CN1863927B (zh) * 2003-09-04 2010-08-11 人类遗传标记控股有限公司 核酸检测分析
CA2544367A1 (en) * 2003-10-29 2005-05-12 Ribomed Biotechnologies, Inc. Compositions, methods and detection technologies for reiterative oligonucleotide synthesis
RU2258741C1 (ru) * 2003-11-18 2005-08-20 Вафин Рамиль Ришадович Способ детекции микроорганизмов с помощью изотермической реакции амплификации-ера (экзонуклеазно-полимеразная амплификация (exonuclease-polimerase amplification)
ATE458044T1 (de) * 2003-12-10 2010-03-15 Takara Bio Inc Verfahren zur nukleinsäureamplifikation
US20050136411A1 (en) * 2003-12-22 2005-06-23 Collins Patrick J. Methods and compositions for linear mRNA amplification from small RNA samples
US8206902B2 (en) * 2003-12-25 2012-06-26 Riken Method of amplifying nucleic acid and method of detecting mutated nucleic acid using the same
WO2005065321A2 (en) 2003-12-29 2005-07-21 Nugen Technologies, Inc. Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids
US8168777B2 (en) 2004-04-29 2012-05-01 Human Genetic Signatures Pty. Ltd. Bisulphite reagent treatment of nucleic acid
DE102004034486B4 (de) * 2004-07-16 2007-08-23 Infineon Technologies Ag Verfahren zum Nachweis von Lumineszenzlicht aus einer porösen Trägerstruktur
DE602005022740D1 (de) * 2004-09-10 2010-09-16 Human Genetic Signatures Pty Amplifikationsblocker umfassend interkalierende nukleinsäuren (ina) enthaltend interkalierende pseudonukleotide (ipn)
ES2399054T3 (es) * 2004-12-03 2013-03-25 Human Genetic Signatures Pty Ltd Métodos para simplificar ácidos nucleicos microbianos mediante modificación química de citosinas
CN101142323A (zh) * 2004-12-23 2008-03-12 人类遗传标记控股有限公司 人类乳头瘤病毒的检测
US7824890B2 (en) * 2005-02-19 2010-11-02 Avacta Group Plc Isothermal amplification of nucleic acids
US8309303B2 (en) 2005-04-01 2012-11-13 Qiagen Gmbh Reverse transcription and amplification of RNA with simultaneous degradation of DNA
AU2006251866B2 (en) * 2005-05-26 2007-11-29 Human Genetic Signatures Pty Ltd Isothermal strand displacement amplification using primers containing a non-regular base
EP1929046B1 (de) * 2005-09-07 2012-07-11 Nugen Technologies, Inc. Verbessertes nukleinsäureamplifikationsverfahren
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
ATE531820T1 (de) * 2005-09-14 2011-11-15 Human Genetic Signatures Pty Gesundheitszustandstest
KR100816419B1 (ko) * 2005-10-14 2008-03-27 래플진(주) 핵산의 등온증폭 방법 및 핵산과 신호 프로브의 동시등온증폭을 이용한 핵산의 검출방법
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
GB0524069D0 (en) 2005-11-25 2006-01-04 Solexa Ltd Preparation of templates for solid phase amplification
DE602006018352D1 (de) 2005-12-06 2010-12-30 Ambion Inc Rückübertragungs-primer und verfahren zu deren entwurf
WO2007073737A1 (en) * 2005-12-29 2007-07-05 Exiqon A/S Detection of tissue origin of cancer
EP2021488B1 (de) 2006-04-28 2018-09-12 Igor Kutyavin Verwendung von basenmodifizierten deoxynukleosidtriphosphaten für verbesserten nukleinsäurenachweis
EP2021506B1 (de) * 2006-04-28 2016-03-16 Igor Kutyavin Verwendung von pcr-amplifikationsprodukten mit elementen sekundärer struktur für verbesserten pcr-basierten nukleinsäurenachweis
KR100785016B1 (ko) * 2006-05-22 2007-12-12 삼성전자주식회사 단일 마이크로 챔버에서 핵산의 농축 및 증폭을 수행하는방법 및 장치
US20080050738A1 (en) * 2006-05-31 2008-02-28 Human Genetic Signatures Pty Ltd. Detection of target nucleic acid
US20100022403A1 (en) * 2006-06-30 2010-01-28 Nurith Kurn Methods for fragmentation and labeling of nucleic acids
US7501254B2 (en) * 2006-07-20 2009-03-10 Ghc Technologies, Inc. Methods and compositions for amplification and capture of nucleic acid sequences
JP4918409B2 (ja) 2006-07-26 2012-04-18 西川ゴム工業株式会社 核酸配列の増幅方法
EP2121983A2 (de) 2007-02-02 2009-11-25 Illumina Cambridge Limited Verfahren zur kennzeichnung von proben und sequenzierung mehrerer nukleotidmatrizen
CA2622649C (en) 2007-03-09 2018-04-24 Riken Nucleic acid amplification method using primer exhibiting exciton effect
JP2010521142A (ja) * 2007-03-16 2010-06-24 ヒューマン ジェネティック シグネチャーズ ピーティーワイ リミテッド 遺伝子発現アッセイ
US8153064B2 (en) 2007-03-22 2012-04-10 Doebler Ii Robert W Systems and devices for isothermal biochemical reactions and/or analysis
US8143006B2 (en) * 2007-08-03 2012-03-27 Igor Kutyavin Accelerated cascade amplification (ACA) of nucleic acids comprising strand and sequence specific DNA nicking
US9388457B2 (en) 2007-09-14 2016-07-12 Affymetrix, Inc. Locus specific amplification using array probes
US8716190B2 (en) 2007-09-14 2014-05-06 Affymetrix, Inc. Amplification and analysis of selected targets on solid supports
CN102124126A (zh) * 2007-10-26 2011-07-13 生命技术公司 使用非随机引物的cdna合成
AU2008329549B2 (en) * 2007-11-27 2013-08-22 Human Genetic Signatures Pty Ltd Enzymes for amplification and copying bisulphite modified nucleic acids
KR100957057B1 (ko) * 2007-12-03 2010-05-13 래플진(주) 핵산과 신호 프로브의 동시 등온증폭을 이용한 핵산의검출방법
US20150079587A1 (en) * 2007-12-03 2015-03-19 Green Cross Medical Science Corp. Method for detecting nucleic acids by simultaneous isothermal amplification of nucleic acids and signal probe
CA2709632A1 (en) * 2007-12-20 2009-07-02 Human Genetic Signatures Pty Ltd Elimination of contaminants associated with nucleic acid amplification
WO2009089466A2 (en) * 2008-01-09 2009-07-16 Keck Graduate Institute System, apparatus and method for material preparation and/or handling
US20090203531A1 (en) 2008-02-12 2009-08-13 Nurith Kurn Method for Archiving and Clonal Expansion
WO2009117698A2 (en) * 2008-03-21 2009-09-24 Nugen Technologies, Inc. Methods of rna amplification in the presence of dna
US8242243B2 (en) 2008-05-15 2012-08-14 Ribomed Biotechnologies, Inc. Methods and reagents for detecting CpG methylation with a methyl CpG binding protein (MBP)
SI2294225T1 (sl) * 2008-06-30 2015-04-30 Life Technologies Corporation Postopek za neposredno amplifikacijo iz vzorcev surove nukleinske kisline
US8211644B2 (en) * 2008-07-13 2012-07-03 Ribomed Biotechnologies, Inc. Molecular beacon-based methods for detection of targets using abscription
WO2010030716A1 (en) * 2008-09-10 2010-03-18 Igor Kutyavin Detection of nucleic acids by oligonucleotide probes cleaved in presence of endonuclease v
WO2010107716A2 (en) 2009-03-15 2010-09-23 Ribomed Biotechnologies, Inc. Abscription based molecular detection
AU2010266034B2 (en) * 2009-06-26 2016-12-15 Claremont Biosolutions Llc Capture and elution of bio-analytes via beads that are used to disrupt specimens
US9914963B2 (en) 2009-09-28 2018-03-13 Igor Kutyavin Methods and compositions for detection of nucleic acids based on stabilized oligonucleotide probe complexes
KR101317709B1 (ko) * 2010-05-14 2013-10-15 (주)진매트릭스 Ｒｎａ 변이 분석방법
DK2753710T3 (en) 2011-09-07 2017-02-06 Human Genetic Signatures Pty Ltd MOLECULAR DETECTION ASSAY
JP5299981B1 (ja) 2011-09-08 2013-09-25 独立行政法人理化学研究所 プライマーセット及びそれを用いた標的核酸配列の増幅方法並びに変異核酸の検出方法
WO2013044097A1 (en) 2011-09-21 2013-03-28 Gen-Probe Incorporated Methods for amplifying nucleic acid using tag-mediated displacement
SG11201401628WA (en) 2011-10-19 2014-05-29 Nugen Technologies Inc Compositions and methods for directional nucleic acid amplification and sequencing
US20130217071A1 (en) 2011-12-30 2013-08-22 Luz Montesclaros Methods and compositions for performing nucleic acid amplification reactions
SG10201504490QA (en) 2012-01-26 2015-07-30 Nugen Technologies Inc Compositions And Methods For Targeted Nucleic Acid Sequence Enrichment And High Efficiency Library Generation
US9957549B2 (en) 2012-06-18 2018-05-01 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
CN102943100B (zh) * 2012-11-23 2014-04-16 郑州安图生物工程股份有限公司 高效RNA逆转录合成cDNA的方法
CN105026576A (zh) * 2012-12-03 2015-11-04 以琳生物药物有限公司 单链多核苷酸扩增方法
US20140274738A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Sequential sequencing
WO2015073711A1 (en) 2013-11-13 2015-05-21 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
WO2015131107A1 (en) 2014-02-28 2015-09-03 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
US10428371B2 (en) 2014-03-31 2019-10-01 Kabushiki Kaisha Dnaform Fluorescent labeled single-stranded nucleic acid and use thereof
EP3169793B1 (de) 2014-07-16 2022-07-06 Tangen Biosciences Inc. Isothermische verfahren zur amplifizierung von nukleinsäureproben
WO2016053883A1 (en) 2014-10-03 2016-04-07 Life Technologies Corporation Detection, identification, validation and enrichment of target nucleic acids
EP3215260B1 (de) 2014-11-03 2020-01-15 Tangen Biosciences Inc. Vorrichtung und verfahren für zellen-, sporen- oder viruserfassung und unterbrechung
GB201617643D0 (en) * 2016-10-18 2016-11-30 Oxford University Innovation Limited Method for complete. uniform and specific amplification of ultra-low amounts of input DNA
US12492430B2 (en) 2017-04-11 2025-12-09 Tecan Genomics, Inc. Library quantitation and qualification
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
US10689629B1 (en) 2017-12-06 2020-06-23 Cepheid Inhibition of nucleic acid polymerases by endonuclease V-cleavable circular oligonucleotide ligands
US10724083B1 (en) 2017-12-06 2020-07-28 Cepheid Inhibition of nucleic acid polymerases by endonuclease V-cleavable oligonucleotide ligands
US10724017B1 (en) 2017-12-06 2020-07-28 Cepheid Inhibition of DNA polymerases by uracil-DNA glycosylase-cleavable oligonucleotide ligands
US12059674B2 (en) 2020-02-03 2024-08-13 Tecan Genomics, Inc. Reagent storage system
JP2025083598A (ja) * 2022-03-31 2025-06-02 国立大学法人埼玉大学 Ｒｎａ／ｄｎａキメラプライマーを用いたｐｃｒ及びその増幅断片連結方法
CN115976170B (zh) * 2022-08-26 2025-09-12 深圳市卓润生物科技有限公司 嵌合引物介导的核酸的检测方法和检测试剂盒

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5011769A (en) * 1985-12-05 1991-04-30 Meiogenics U.S. Limited Partnership Methods for detecting nucleic acid sequences
US4876187A (en) * 1985-12-05 1989-10-24 Meiogenics, Inc. Nucleic acid compositions with scissile linkage useful for detecting nucleic acid sequences
FR2663949A1 (fr) * 1990-06-29 1992-01-03 Genset Sa Perfectionnements a un procede d'amplification enzymatique in vitro.
AU8997991A (en) * 1991-01-31 1992-08-06 Becton Dickinson & Company Exonuclease mediated strand displacement amplification
WO1993009250A1 (en) * 1991-11-01 1993-05-13 Adelaide Children's Hospital Solid phase amplification process
US5270184A (en) * 1991-11-19 1993-12-14 Becton, Dickinson And Company Nucleic acid target generation
JPH06327500A (ja) * 1993-05-20 1994-11-29 Toyobo Co Ltd 核酸配列の増幅方法および検出方法
FR2708288B1 (fr) * 1993-07-26 1995-09-01 Bio Merieux Procédé d'amplification d'acides nucléiques par transcription utilisant le déplacement, réactifs et nécessaire pour la mise en Óoeuvre de ce procédé.
CA2140081C (en) * 1994-01-13 2008-04-01 Dean L. Engelhardt Process, construct and conjugate for producing multiple nucleic acid copies
FR2724934B1 (fr) * 1994-09-26 1997-01-24 Bio Merieux Oligonucleotide chimere et son utilisation dans l'obtention de transcrits d'un acide nucleique

Also Published As

Publication number Publication date
DE69619959D1 (de) 2002-04-25
EP0787209B1 (de) 2002-03-20
FR2737223B1 (fr) 1997-09-12
EP0787209A1 (de) 1997-08-06
WO1997004126A1 (fr) 1997-02-06
DE69619959T2 (de) 2002-11-07
CA2200627A1 (fr) 1997-02-06
US5824517A (en) 1998-10-20
CA2200627C (fr) 2011-05-10
FR2737223A1 (fr) 1997-01-31

Similar Documents

Publication Publication Date Title
ATE214741T1 (de) Verfahren zur amplifizierung von nukleinsäuse sequenzen durch verdrängung mit hilfe von chimären primer
WO1997004126B1 (fr) Procede d'amplification de sequences d'acide nucleique par deplacement, a l'aide d'amorces chimeres
ATE207543T1 (de) Isotherme amplifikation von nukleinsäuren durch strangverdrängung
WO1995003426A3 (fr) Procede d'amplification d'acides nucleiques par transcription utilisant le deplacement, reactifs et necessaire pour la mise en ×uvre de ce procede
ATE205543T1 (de) Verfahren zur verbesserung der nukleinsäureamplifizierung
DK0721988T3 (da) Kimærisk oligonucleotid og dets anvendelse ved opnåelse af transkripter af en nucleinsyre
WO2003020891A3 (en) Single nucleotide amplification and detection by polymerase
GB9210176D0 (en) Chemical method
EP0822256A3 (de) Modifizierte thermostabile DNA Polymerase und eine DNA Polymerasezusammensetzung zur Amplifikation von Nukleinsäuren
DE69737450D1 (de) Sequenzierung durch ligation kodierter adapter
WO1995013396A3 (en) A method for identifying microorganisms, and aids useful thereof
IL102765A (en) Reagents and methods to detect and determine mycobacterium nucleic acids
WO1997041260A3 (en) Subtractive amplification
DE69329636D1 (de) Verfahren zur Vervielfältigung von Nukleinsäuresequenzen
ATE238434T1 (de) Eine in kaskaden verlaufende vervielfältigungsreaktion von nukleinsäuren
FI972238A0 (fi) Terminaaliseen toistumasekvenssiin perustuva monistusmenetelmä
ATE403747T1 (de) Verfahren zur amplifikation von signal- flankierenden sequenzen aus unbekannten, genomischen daten
EP0745675A3 (de) Methode zur Amplifizierung von Nukleinsäure und entsprechende Regenzien dafür
ATE203775T1 (de) Methode zur detektion einer gezielten nukleinsäure
ATE440147T1 (de) Erhíhung der spezifität von nukleinsäureamplifizierungen durch trägernukleinsäuren
SE9403805D0 (sv) Method of preparing oligonucleotide probes or primers, vector therefor and use thereof
EP1251182A3 (de) Verfahren zur Amplifikation und Erkennung von Nukleinsäure-Zielsequenzen unter Verwendung von thermostabilen Enzymen
AU8092791A (en) Improved in vitro enzymatic amplification method
ATE180020T1 (de) Verfahren zur früherkennung von hpv-assoziierten karzinomen bzw. von hochgradigen, durch hpv- verursachte dysplasien
DE50209094D1 (de) Vermehrung von ribonukleinsäuren

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties