ATE220725T1 - Glycosylase verwendende detektion von bestimmten nukleinsäuresequenzen - Google Patents
Glycosylase verwendende detektion von bestimmten nukleinsäuresequenzenInfo
- Publication number
- ATE220725T1 ATE220725T1 AT95942776T AT95942776T ATE220725T1 AT E220725 T1 ATE220725 T1 AT E220725T1 AT 95942776 T AT95942776 T AT 95942776T AT 95942776 T AT95942776 T AT 95942776T AT E220725 T1 ATE220725 T1 AT E220725T1
- Authority
- AT
- Austria
- Prior art keywords
- glycosylase
- pct
- detection
- dna
- nucleic acid
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IE950526 | 1995-07-11 | ||
| PCT/IE1995/000067 WO1997003210A1 (en) | 1995-07-11 | 1995-12-21 | Glycosylase mediated detection of nucleotide sequences at candidate loci |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE220725T1 true ATE220725T1 (de) | 2002-08-15 |
Family
ID=11040818
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT95942776T ATE220725T1 (de) | 1995-07-11 | 1995-12-21 | Glycosylase verwendende detektion von bestimmten nukleinsäuresequenzen |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US5952176A (de) |
| EP (1) | EP0843735B2 (de) |
| JP (1) | JP3790797B2 (de) |
| AT (1) | ATE220725T1 (de) |
| AU (1) | AU708821B2 (de) |
| CA (1) | CA2226542C (de) |
| DE (1) | DE69527444T3 (de) |
| DK (1) | DK0843735T4 (de) |
| ES (1) | ES2176358T3 (de) |
| NO (1) | NO980105L (de) |
| NZ (1) | NZ298494A (de) |
| PT (1) | PT843735E (de) |
| WO (1) | WO1997003210A1 (de) |
Families Citing this family (58)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6190865B1 (en) | 1995-09-27 | 2001-02-20 | Epicentre Technologies Corporation | Method for characterizing nucleic acid molecules |
| SE9601676D0 (sv) | 1996-04-30 | 1996-04-30 | Ulf Landegren | Improved probing of specific mucleic acids |
| US6117634A (en) * | 1997-03-05 | 2000-09-12 | The Reagents Of The University Of Michigan | Nucleic acid sequencing and mapping |
| US6197557B1 (en) | 1997-03-05 | 2001-03-06 | The Regents Of The University Of Michigan | Compositions and methods for analysis of nucleic acids |
| US5885958A (en) * | 1997-03-25 | 1999-03-23 | Administrators Of The Tulane Educational Fund | Mu-opiate receptor peptides |
| US6994960B1 (en) | 1997-05-28 | 2006-02-07 | The Walter And Eliza Hall Institute Of Medical Research | Nucleic acid diagnostics based on mass spectrometry or mass separation and base specific cleavage |
| US6090553A (en) * | 1997-10-29 | 2000-07-18 | Beckman Coulter, Inc. | Use of uracil-DNA glycosylase in genetic analysis |
| AU756384B2 (en) * | 1998-02-23 | 2003-01-09 | Dana-Farber Cancer Institute, Inc. | Method for identifying mismatch repair glycosylase reactive sites, compound and uses thereof |
| DK1071811T3 (da) * | 1998-04-22 | 2002-06-17 | Entpr Ie Trd As Bioresearch Ie | Fremgangsmåde til karakterisering af nukleinsyremolekyler, hvilken fremgangsmåde involverer generering af forlængelige opstrøms-DNA-fragmenter, som er et resultat af spaltningen af nukleinsyre i et abasisk sted |
| US6048696A (en) * | 1998-05-13 | 2000-04-11 | Epicentre Technologies Corporation | Method of identifying nucleic acid molecules |
| US7270958B2 (en) | 1998-09-10 | 2007-09-18 | The Regents Of The University Of Michigan | Compositions and methods for analysis of nucleic acids |
| US6440705B1 (en) * | 1998-10-01 | 2002-08-27 | Vincent P. Stanton, Jr. | Method for analyzing polynucleotides |
| EP1041159A3 (de) * | 1999-03-26 | 2000-10-25 | MIRA Diagnostika GmbH | Verfahren, Oligonucleotide zum Abbau von in-vitro synthetisierten Nucleinsäuremolekülen |
| US20060275782A1 (en) | 1999-04-20 | 2006-12-07 | Illumina, Inc. | Detection of nucleic acid reactions on bead arrays |
| US20030215821A1 (en) * | 1999-04-20 | 2003-11-20 | Kevin Gunderson | Detection of nucleic acid reactions on bead arrays |
| US20030207297A1 (en) * | 1999-10-13 | 2003-11-06 | Hubert Koster | Methods for generating databases and databases for identifying polymorphic genetic markers |
| US7332275B2 (en) | 1999-10-13 | 2008-02-19 | Sequenom, Inc. | Methods for detecting methylated nucleotides |
| AU776811C (en) | 1999-10-13 | 2005-07-28 | Agena Bioscience, Inc. | Methods for generating databases and databases for identifying polymorphic genetic markers |
| US6958214B2 (en) | 2000-07-10 | 2005-10-25 | Sequenom, Inc. | Polymorphic kinase anchor proteins and nucleic acids encoding the same |
| DE60131903T2 (de) * | 2000-10-24 | 2008-11-27 | The Board of Trustees of the Leland S. Stanford Junior University, Palo Alto | Direkte multiplex charakterisierung von genomischer dna |
| EP1332208A2 (de) * | 2000-10-30 | 2003-08-06 | Gene Logic, Inc. | Teilweise doppelsträngige nukleinsäuren, verfahren zu ihrer herstellung und ihre verwendung |
| IE20000887A1 (en) * | 2000-11-03 | 2002-12-11 | Univ College Cork Nat Univ Ie | Method for the amplification and optional characterisation of nucleic acids |
| WO2002081753A1 (en) * | 2001-04-04 | 2002-10-17 | Advanced Research & Technology Institute | Method for identifying and characterizing individual dna molecules |
| US7208295B2 (en) * | 2001-11-19 | 2007-04-24 | Affymetrix, Inc. | Multiplex oligonucleotide addition and target amplification |
| US20110151438A9 (en) | 2001-11-19 | 2011-06-23 | Affymetrix, Inc. | Methods of Analysis of Methylation |
| US7435572B2 (en) * | 2002-04-12 | 2008-10-14 | New England Biolabs, Inc. | Methods and compositions for DNA manipulation |
| CA2484676A1 (en) | 2002-05-03 | 2003-11-13 | Sequenom, Inc. | Kinase anchor protein muteins, peptides thereof, and related methods |
| WO2004011665A2 (en) * | 2002-05-17 | 2004-02-05 | Nugen Technologies, Inc. | Methods for fragmentation, labeling and immobilization of nucleic acids |
| IE20020544A1 (en) * | 2002-06-28 | 2003-12-31 | Univ College Cork Nat Univ Ie | Method for the characterisation of nucleic acid molecules |
| CN1774511B (zh) | 2002-11-27 | 2013-08-21 | 斯昆诺有限公司 | 用于序列变异检测和发现的基于断裂的方法和系统 |
| CA2515583C (en) | 2003-03-07 | 2015-07-14 | Diversa Corporation | Hydrolases, nucleic acids encoding them and methods for making and using them |
| US20050239087A1 (en) * | 2003-04-29 | 2005-10-27 | Haiguang Xiao | Multiply-primed amplification of nucleic acid sequences |
| US20050181394A1 (en) * | 2003-06-20 | 2005-08-18 | Illumina, Inc. | Methods and compositions for whole genome amplification and genotyping |
| AU2004253882B2 (en) | 2003-06-20 | 2010-06-10 | Illumina, Inc. | Methods and compositions for whole genome amplification and genotyping |
| WO2005024068A2 (en) | 2003-09-05 | 2005-03-17 | Sequenom, Inc. | Allele-specific sequence variation analysis |
| CA2552007A1 (en) | 2003-12-29 | 2005-07-21 | Nugen Technologies, Inc. | Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids |
| EP1727911B1 (de) | 2004-03-26 | 2013-01-23 | Sequenom, Inc. | Basenspezifische spaltung methylierungsspezifischer amplifikationsprodukte in kombination mit massenanalyse |
| US7608394B2 (en) | 2004-03-26 | 2009-10-27 | Sequenom, Inc. | Methods and compositions for phenotype identification based on nucleic acid methylation |
| WO2008005459A2 (en) * | 2006-06-30 | 2008-01-10 | Nugen Technologies, Inc. | Methods for fragmentation and labeling of nucleic acids |
| EP2145180B1 (de) * | 2007-04-13 | 2013-12-04 | Sequenom, Inc. | Vergleichende sequenzanalyseverfahren und -systeme |
| US8101353B2 (en) * | 2007-12-18 | 2012-01-24 | Advanced Analytical Technologies, Inc. | System and method for nucleotide sequence profiling for sample identification |
| US12527665B2 (en) | 2008-12-11 | 2026-01-20 | Pacific Biosciences Of California, Inc. | Methods for identifying modified bases in nucleic acid templates |
| AU2009325069B2 (en) | 2008-12-11 | 2015-03-19 | Pacific Biosciences Of California, Inc. | Classification of nucleic acid templates |
| US9175338B2 (en) | 2008-12-11 | 2015-11-03 | Pacific Biosciences Of California, Inc. | Methods for identifying nucleic acid modifications |
| US8889353B2 (en) | 2009-09-23 | 2014-11-18 | Koninklijke Philips N.V. | Method for monitoring the bisulfite-mediated conversion of DNA |
| EP2769007B1 (de) | 2011-10-19 | 2016-12-07 | Nugen Technologies, Inc. | Zusammensetzungen und verfahren zur direktionalen amplifikation und sequenzierung von nukleinsäuren |
| CN105861487B (zh) | 2012-01-26 | 2020-05-05 | 纽亘技术公司 | 用于靶向核酸序列富集和高效文库产生的组合物和方法 |
| WO2013163207A1 (en) | 2012-04-24 | 2013-10-31 | Pacific Biosciences Of California, Inc. | Identification of 5-methyl-c in nucleic acid templates |
| CA2877094A1 (en) | 2012-06-18 | 2013-12-27 | Nugen Technologies, Inc. | Compositions and methods for negative selection of non-desired nucleic acid sequences |
| US20150011396A1 (en) | 2012-07-09 | 2015-01-08 | Benjamin G. Schroeder | Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing |
| EP2971130A4 (de) | 2013-03-15 | 2016-10-05 | Nugen Technologies Inc | Sequentielle sequenzierung |
| JP6525473B2 (ja) | 2013-11-13 | 2019-06-05 | ニューゲン テクノロジーズ， インコーポレイテッド | 複製物配列決定リードを同定するための組成物および方法 |
| WO2015131107A1 (en) | 2014-02-28 | 2015-09-03 | Nugen Technologies, Inc. | Reduced representation bisulfite sequencing with diversity adaptors |
| EP3177740B1 (de) | 2014-08-06 | 2021-01-13 | Nugen Technologies, Inc. | Digitale messungen von gezielter sequenzierung |
| US12492430B2 (en) | 2017-04-11 | 2025-12-09 | Tecan Genomics, Inc. | Library quantitation and qualification |
| US11099202B2 (en) | 2017-10-20 | 2021-08-24 | Tecan Genomics, Inc. | Reagent delivery system |
| EP3851542A1 (de) | 2020-01-20 | 2021-07-21 | Tecan Genomics, Inc. | Verarmung von reichlich uninformativen sequenzen |
| CN113275053A (zh) | 2020-02-03 | 2021-08-20 | 帝肯基因组学公司 | 试剂存储系统 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0540693B1 (de) * | 1990-07-24 | 1999-01-20 | F. Hoffmann-La Roche Ag | Verringerung von nicht spezifischer amplifikation während einer (in vitro) nukleinsäure amplifikation unter verwendung von modifizierten nukleinsäure basen |
| US6190865B1 (en) † | 1995-09-27 | 2001-02-20 | Epicentre Technologies Corporation | Method for characterizing nucleic acid molecules |
-
1995
- 1995-12-21 EP EP95942776A patent/EP0843735B2/de not_active Expired - Lifetime
- 1995-12-21 PT PT95942776T patent/PT843735E/pt unknown
- 1995-12-21 CA CA002226542A patent/CA2226542C/en not_active Expired - Fee Related
- 1995-12-21 AU AU43995/96A patent/AU708821B2/en not_active Ceased
- 1995-12-21 US US08/981,663 patent/US5952176A/en not_active Expired - Fee Related
- 1995-12-21 AT AT95942776T patent/ATE220725T1/de not_active IP Right Cessation
- 1995-12-21 ES ES95942776T patent/ES2176358T3/es not_active Expired - Lifetime
- 1995-12-21 NZ NZ298494A patent/NZ298494A/en unknown
- 1995-12-21 WO PCT/IE1995/000067 patent/WO1997003210A1/en not_active Ceased
- 1995-12-21 DK DK95942776T patent/DK0843735T4/da active
- 1995-12-21 DE DE69527444T patent/DE69527444T3/de not_active Expired - Fee Related
- 1995-12-21 JP JP50564497A patent/JP3790797B2/ja not_active Expired - Fee Related
-
1998
- 1998-01-09 NO NO980105A patent/NO980105L/no not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| EP0843735B1 (de) | 2002-07-17 |
| EP0843735A1 (de) | 1998-05-27 |
| ES2176358T3 (es) | 2002-12-01 |
| AU4399596A (en) | 1997-02-10 |
| DE69527444T2 (de) | 2003-02-20 |
| JP3790797B2 (ja) | 2006-06-28 |
| WO1997003210A1 (en) | 1997-01-30 |
| DE69527444D1 (de) | 2002-08-22 |
| NO980105D0 (no) | 1998-01-09 |
| CA2226542C (en) | 2006-04-04 |
| CA2226542A1 (en) | 1997-01-30 |
| NZ298494A (en) | 1999-09-29 |
| DE69527444T3 (de) | 2007-08-16 |
| US5952176A (en) | 1999-09-14 |
| JP2000509241A (ja) | 2000-07-25 |
| NO980105L (no) | 1998-03-10 |
| EP0843735B2 (de) | 2006-12-27 |
| DK0843735T3 (da) | 2002-11-11 |
| PT843735E (pt) | 2002-11-29 |
| AU708821B2 (en) | 1999-08-12 |
| DK0843735T4 (da) | 2007-01-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE69527444D1 (de) | Glycosylase verwendende detektion von bestimmten nukleinsäuresequenzen | |
| Trinh et al. | DNA methylation analysis by MethyLight technology | |
| US5536649A (en) | Decontamination of nucleic acid amplification reactions using uracil-N-glycosylase (UDG) | |
| DE69329800D1 (de) | Detektion von gensequenzen in biologischen flüssigkeiten | |
| AU2180200A (en) | A heterogeneous assay for pyrophosphate detection | |
| DE60116651D1 (de) | Methode zur amplifizierung und möglicher charakterisierung von nukleinsäuren | |
| DE69333073D1 (de) | Verwendungen von fluoreszierenden n-nukleosiden und dessen analogen | |
| DE69931646D1 (de) | Multiplex genotypisierung unter verwendung von fluoreszenzmarkierten hybridisierungssonden | |
| ATE422557T1 (de) | Nachweissysteme auf festen trägern und methoden zur verwendung von nicht-standardbasen | |
| MX9705961A (es) | Polimerasa de adn termoestable, modificada. | |
| ATE458068T1 (de) | Einzelnukleotidamplifikation und -nachweis mit polymerase | |
| AU8132194A (en) | Single nucleotide polymorphisms and their use in genetic analysis | |
| DE69637726D1 (de) | Gruppen zum einfangen von nukleinsäuren | |
| ATE542916T1 (de) | Methoden zur erzeugung von oligonukleotidlösungen | |
| DE69636895D1 (de) | Die Verwendung der 3'-wesentlichen Bearbeitungswirksamkeit von DNS-Polymerase | |
| ATE532877T1 (de) | Nukleinsäuremoleküle zum nachweis von bakterien und phylogenetischen einheiten von bakterien | |
| ATE160822T1 (de) | Identifizierung von salmonella durch die polymerase-ketten-reaktion | |
| NO20005428D0 (no) | Varmelabil uracil-DNA-glykosylase samt anvendelse derav | |
| DE60132924D1 (de) | Zusammensetzungen und methoden zur detektion von humanem immundefizienz virus 2 (hiv-2) | |
| ATE227345T1 (de) | Verfahren zur gattungs- und speziesspezifische identifizierung von legionellen | |
| DE59712871D1 (de) | Heterocyclische verbindungen und deren verwendung in der detektion von nucleinsäuren | |
| DE50209977D1 (de) | Nachweis und differenzierung von mikroorganismen der spezies yersinia pestis/yersinia pseudotuberculosis | |
| FR2774097B1 (fr) | Utilisation de derives d'indolamine dans la detection d'une activite de peptidase ou dans la detection de micro-organismes | |
| ATE211507T1 (de) | Nachweis von nukleotidsequenzen durch signalamplifizierung | |
| ATE225404T1 (de) | Verfahren und zusammensetzungen zum nachweis von spleiss-mutationen in dihydropyremudine dehydrogenase |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification | ||
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |