ATE223491T1 - Verfahren zur reinigung von biomolekül- oder proteinkomplexen - Google Patents

Verfahren zur reinigung von biomolekül- oder proteinkomplexen

Info

Publication number: ATE223491T1
Authority: AT; Austria
Prior art keywords: biomolecule; purifying; protein complexes; vectors; detecting
Prior art date: 1998-08-17

Application number

AT99944420T

Other languages

English (en)

Inventor

Bertrand Seraphin

Guillaume Rigaut

Original Assignee

Europ Lab Molekularbiolog

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1998-08-17

Filing date

1999-08-17

Publication date

2002-09-15

1999-08-17 Application filed by Europ Lab Molekularbiolog filed Critical Europ Lab Molekularbiolog

2002-09-15 Application granted granted Critical

2002-09-15 Publication of ATE223491T1 publication Critical patent/ATE223491T1/de

Links

102000007474 Multiprotein Complexes Human genes 0.000 title abstract 2
108010085220 Multiprotein Complexes Proteins 0.000 title abstract 2
102000037865 fusion proteins Human genes 0.000 abstract 1
108020001507 fusion proteins Proteins 0.000 abstract 1
102000039446 nucleic acids Human genes 0.000 abstract 1
108020004707 nucleic acids Proteins 0.000 abstract 1
150000007523 nucleic acids Chemical class 0.000 abstract 1
102000004169 proteins and genes Human genes 0.000 abstract 1
108090000623 proteins and genes Proteins 0.000 abstract 1
239000013598 vector Substances 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/705—Fusion polypeptide containing domain for protein-protein interaction containing a protein-A fusion

Landscapes

Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Biomedical Technology (AREA)
Wood Science & Technology (AREA)
Organic Chemistry (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Chemical & Material Sciences (AREA)
Bioinformatics & Cheminformatics (AREA)
Zoology (AREA)
Molecular Biology (AREA)
Biophysics (AREA)
Microbiology (AREA)
Plant Pathology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Physics & Mathematics (AREA)
Peptides Or Proteins (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Investigating Or Analysing Biological Materials (AREA)

AT99944420T 1998-08-17 1999-08-17 Verfahren zur reinigung von biomolekül- oder proteinkomplexen ATE223491T1 (de)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
EP98115448		1998-08-17
PCT/EP1999/006022 WO2000009716A1 (en)	1998-08-17	1999-08-17	Method for purifying proteins and/or biomolecule or protein complexes

Publications (1)

Publication Number	Publication Date
ATE223491T1 true ATE223491T1 (de)	2002-09-15

Family

ID=8232468

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT99944420T ATE223491T1 (de)	1998-08-17	1999-08-17	Verfahren zur reinigung von biomolekül- oder proteinkomplexen

Country Status (11)

Country	Link
US (2)	US20020061513A1 (de)
EP (2)	EP1231276A1 (de)
JP (1)	JP3676978B2 (de)
AT (1)	ATE223491T1 (de)
AU (1)	AU762961B2 (de)
CA (1)	CA2340974C (de)
DE (2)	DE69902796T2 (de)
DK (1)	DK1105508T3 (de)
ES (1)	ES2183601T3 (de)
PT (1)	PT1105508E (de)
WO (1)	WO2000009716A1 (de)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US7192596B2 (en)	1996-08-23	2007-03-20	The Health Protection Agency Ipsen Limited	Recombinant toxin fragments
ES2183601T3 (es) *	1998-08-17	2003-03-16	Europ Lab Molekularbiolog	Metodo para purificar complejos de biomoleculas o proteinas.
FR2830020B1 (fr) *	2001-09-25	2003-12-19	Univ Victor Segalen Bordeaux 2	Acide nucleique pour l'obtention d'un polynucleotide codant une proteine de detection et d'isolement d'un ligand "proie" et applications de cet acide nucleique
US7825227B2 (en) *	2002-05-09	2010-11-02	Prolexys Pharmaceuticals, Inc.	Method for purification of a protein complex and identification of its components
CA2463301A1 (en) *	2004-04-22	2005-10-22	Atanas Iliev Lalev	Method for detecting protein interactions - affinity purification with switching the ligand
WO2005023858A1 (en) *	2003-09-05	2005-03-17	Cellzome Ag	Protein complexes associated with app-processing
US7238478B2 (en)	2003-11-13	2007-07-03	Stratagene California	Compositions and methods for protein isolation
WO2006070416A1 (en) *	2004-12-30	2006-07-06	Fondazione Santa Lucia I.R.C.C.S.	An immunoprecipitation-based method to purify and characterise biological macromolecular complexes
EP1841874B1 (de) *	2005-01-27	2010-01-06	Five Prime Therapeutics, Inc.	Leitsequenzen zur anleitung von polypeptidsekretion und verfahren zur herstellung
CN102414218A (zh)	2009-04-22	2012-04-11	国立大学法人大阪大学	具有蛋白酶识别序列的标签肽及其应用
FR2944799B1 (fr) *	2009-04-28	2011-05-13	Ecole De Biolog Ind Ebi	Procede de production d'une proteine d'interet grace a une nouvelle proteine de fusion.
WO2016145416A2 (en) *	2015-03-11	2016-09-15	The Broad Institute, Inc.	Proteomic analysis with nucleic acid identifiers
WO2019183334A1 (en)	2018-03-21	2019-09-26	Waters Technologies Corporation	Non-antibody high-affinity-based sample preparation, sorbents, devices and methods
CN119978097A (zh) *	2025-01-13	2025-05-13	武汉爱博泰克生物科技有限公司	小鼠白细胞介素23异源二聚体的制备方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5821088A (en) *	1990-05-11	1998-10-13	Siga Pharmaceuticals, Inc.	Use of gram-positive bacteria to express recombinant proteins
ES2183601T3 (es) *	1998-08-17	2003-03-16	Europ Lab Molekularbiolog	Metodo para purificar complejos de biomoleculas o proteinas.

1999
- 1999-08-17 ES ES99944420T patent/ES2183601T3/es not_active Expired - Lifetime
- 1999-08-17 AU AU57362/99A patent/AU762961B2/en not_active Ceased
- 1999-08-17 DE DE69902796T patent/DE69902796T2/de not_active Expired - Lifetime
- 1999-08-17 EP EP02004765A patent/EP1231276A1/de not_active Withdrawn
- 1999-08-17 EP EP99944420A patent/EP1105508B1/de not_active Expired - Lifetime
- 1999-08-17 CA CA002340974A patent/CA2340974C/en not_active Expired - Fee Related
- 1999-08-17 JP JP2000565150A patent/JP3676978B2/ja not_active Expired - Fee Related
- 1999-08-17 AT AT99944420T patent/ATE223491T1/de active
- 1999-08-17 PT PT99944420T patent/PT1105508E/pt unknown
- 1999-08-17 DK DK99944420T patent/DK1105508T3/da active
- 1999-08-17 WO PCT/EP1999/006022 patent/WO2000009716A1/en not_active Ceased
- 1999-08-17 DE DE1105508T patent/DE1105508T1/de active Pending
2001
- 2001-02-16 US US09/785,793 patent/US20020061513A1/en not_active Abandoned
2008
- 2008-06-03 US US12/156,626 patent/US20090042216A1/en not_active Abandoned

Also Published As

Publication number	Publication date
AU762961B2 (en)	2003-07-10
CA2340974A1 (en)	2000-02-24
JP3676978B2 (ja)	2005-07-27
DE69902796T2 (de)	2003-08-07
US20020061513A1 (en)	2002-05-23
US20090042216A1 (en)	2009-02-12
JP2002522085A (ja)	2002-07-23
EP1105508A1 (de)	2001-06-13
CA2340974C (en)	2010-01-12
DE1105508T1 (de)	2002-02-21
DE69902796D1 (de)	2002-10-10
WO2000009716A1 (en)	2000-02-24
PT1105508E (pt)	2003-01-31
EP1231276A1 (de)	2002-08-14
DK1105508T3 (da)	2003-01-06
ES2183601T3 (es)	2003-03-16
AU5736299A (en)	2000-03-06
EP1105508B1 (de)	2002-09-04

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DE3585147D1 (de)	1992-02-20	Verfahren zur reinigung von proteinen.
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