ES2183601T3 - Metodo para purificar complejos de biomoleculas o proteinas. - Google Patents

Metodo para purificar complejos de biomoleculas o proteinas.

Info

Publication number
ES2183601T3
ES2183601T3 ES99944420T ES99944420T ES2183601T3 ES 2183601 T3 ES2183601 T3 ES 2183601T3 ES 99944420 T ES99944420 T ES 99944420T ES 99944420 T ES99944420 T ES 99944420T ES 2183601 T3 ES2183601 T3 ES 2183601T3
Authority
ES
Spain
Prior art keywords
subunits
support material
affinity
proteins
expression
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES99944420T
Other languages
English (en)
Inventor
Bertrand Seraphin
Guillaume Rigaut
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Europaisches Laboratorium fuer Molekularbiologie EMBL
Original Assignee
Europaisches Laboratorium fuer Molekularbiologie EMBL
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Europaisches Laboratorium fuer Molekularbiologie EMBL filed Critical Europaisches Laboratorium fuer Molekularbiologie EMBL
Application granted granted Critical
Publication of ES2183601T3 publication Critical patent/ES2183601T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/705—Fusion polypeptide containing domain for protein-protein interaction containing a protein-A fusion

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Método para detectar y/o purificar complejos de biomoléculas y/o proteínas que comprende los pasos: (a) proporcionar un ambiente de expresión que contiene uno o más ácidos nucleicos heterólogos que codifican una o más subunidades de un complejo de biomoléculas, estando las subunidades fusionadas a al menos dos marcadores de afinidad diferentes, uno de los cuales está constituido por uno o más dominios de fijación de IgG de la proteína A de Staphylococcus, (b) mantener el ambiente de expresión en condiciones que facilitan la expresión de la una o más subunidades en una forma nativa como proteínas de fusión con los marcadores de afinidad, y en condiciones que permiten la formación de un complejo entre la una o más subunidades y posiblemente otros componentes capaces de complejarse con la una o más subunidades, (c) detectar y/o purificar la una o más subunidades por una combinación de al menos dos pasos de purificación por afinidad diferentes cada uno de los cuales comprende fijar la una omás subunidades por la vía de un marcador de afinidad a un material soporte capaz de fijar selectivamente uno de los marcadores de afinidad y separar la una o más subunidades del material soporte después que las sustancias no fijadas al material soporte se han eliminado.
ES99944420T 1998-08-17 1999-08-17 Metodo para purificar complejos de biomoleculas o proteinas. Expired - Lifetime ES2183601T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP98115448 1998-08-17

Publications (1)

Publication Number Publication Date
ES2183601T3 true ES2183601T3 (es) 2003-03-16

Family

ID=8232468

Family Applications (1)

Application Number Title Priority Date Filing Date
ES99944420T Expired - Lifetime ES2183601T3 (es) 1998-08-17 1999-08-17 Metodo para purificar complejos de biomoleculas o proteinas.

Country Status (11)

Country Link
US (2) US20020061513A1 (es)
EP (2) EP1105508B1 (es)
JP (1) JP3676978B2 (es)
AT (1) ATE223491T1 (es)
AU (1) AU762961B2 (es)
CA (1) CA2340974C (es)
DE (2) DE1105508T1 (es)
DK (1) DK1105508T3 (es)
ES (1) ES2183601T3 (es)
PT (1) PT1105508E (es)
WO (1) WO2000009716A1 (es)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7192596B2 (en) 1996-08-23 2007-03-20 The Health Protection Agency Ipsen Limited Recombinant toxin fragments
DK1105508T3 (da) * 1998-08-17 2003-01-06 Europ Lab Molekularbiolog Fremgangsmåde til oprensning af biomolekyle- eller proteinkomplekser
FR2830020B1 (fr) * 2001-09-25 2003-12-19 Univ Victor Segalen Bordeaux 2 Acide nucleique pour l'obtention d'un polynucleotide codant une proteine de detection et d'isolement d'un ligand "proie" et applications de cet acide nucleique
US7825227B2 (en) * 2002-05-09 2010-11-02 Prolexys Pharmaceuticals, Inc. Method for purification of a protein complex and identification of its components
CA2463301A1 (en) * 2004-04-22 2005-10-22 Atanas Iliev Lalev Method for detecting protein interactions - affinity purification with switching the ligand
AU2003298171A1 (en) * 2003-09-05 2005-03-29 Cellzome Ag Protein complexes associated with app-processing
US7238478B2 (en) 2003-11-13 2007-07-03 Stratagene California Compositions and methods for protein isolation
US20080254551A1 (en) * 2004-12-30 2008-10-16 Achsel Tilmann Immunoprecipitaion-Based Method to Purify and Characterise Biological Macromolecular Complexes
CA2589895A1 (en) * 2005-01-27 2006-08-03 Five Prime Therapeutics, Inc. Leader sequences for directing secretion of polypeptides and methods for production thereof
EP2423218B1 (en) 2009-04-22 2014-06-11 Osaka University Tag peptide having protease recognition sequence and utilization of same
FR2944799B1 (fr) 2009-04-28 2011-05-13 Ecole De Biolog Ind Ebi Procede de production d'une proteine d'interet grace a une nouvelle proteine de fusion.
WO2016145416A2 (en) * 2015-03-11 2016-09-15 The Broad Institute, Inc. Proteomic analysis with nucleic acid identifiers
WO2019183334A1 (en) 2018-03-21 2019-09-26 Waters Technologies Corporation Non-antibody high-affinity-based sample preparation, sorbents, devices and methods
CN119978097A (zh) * 2025-01-13 2025-05-13 武汉爱博泰克生物科技有限公司 小鼠白细胞介素23异源二聚体的制备方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5821088A (en) * 1990-05-11 1998-10-13 Siga Pharmaceuticals, Inc. Use of gram-positive bacteria to express recombinant proteins
DK1105508T3 (da) * 1998-08-17 2003-01-06 Europ Lab Molekularbiolog Fremgangsmåde til oprensning af biomolekyle- eller proteinkomplekser

Also Published As

Publication number Publication date
US20090042216A1 (en) 2009-02-12
AU762961B2 (en) 2003-07-10
JP2002522085A (ja) 2002-07-23
DE69902796T2 (de) 2003-08-07
EP1105508A1 (en) 2001-06-13
JP3676978B2 (ja) 2005-07-27
PT1105508E (pt) 2003-01-31
DE1105508T1 (de) 2002-02-21
CA2340974A1 (en) 2000-02-24
EP1105508B1 (en) 2002-09-04
EP1231276A1 (en) 2002-08-14
US20020061513A1 (en) 2002-05-23
AU5736299A (en) 2000-03-06
DK1105508T3 (da) 2003-01-06
CA2340974C (en) 2010-01-12
ATE223491T1 (de) 2002-09-15
DE69902796D1 (de) 2002-10-10
WO2000009716A1 (en) 2000-02-24

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