ATE224457T1 - Verfahren zur identifizierung von nukleinsäuren, welche agenzien kodieren, die zelluläre phenotypen beeinflussen - Google Patents

Verfahren zur identifizierung von nukleinsäuren, welche agenzien kodieren, die zelluläre phenotypen beeinflussen

Info

Publication number
ATE224457T1
ATE224457T1 AT98911497T AT98911497T ATE224457T1 AT E224457 T1 ATE224457 T1 AT E224457T1 AT 98911497 T AT98911497 T AT 98911497T AT 98911497 T AT98911497 T AT 98911497T AT E224457 T1 ATE224457 T1 AT E224457T1
Authority
AT
Austria
Prior art keywords
expression
reporter
library
phenotype
identifying nucleic
Prior art date
Application number
AT98911497T
Other languages
English (en)
Inventor
Carl Alexander Kamb
Original Assignee
Deltagen Proteomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Deltagen Proteomics Inc filed Critical Deltagen Proteomics Inc
Application granted granted Critical
Publication of ATE224457T1 publication Critical patent/ATE224457T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1051—Gene trapping, e.g. exon-, intron-, IRES-, signal sequence-trap cloning, trap vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT98911497T 1997-03-04 1998-02-27 Verfahren zur identifizierung von nukleinsäuren, welche agenzien kodieren, die zelluläre phenotypen beeinflussen ATE224457T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/812,994 US5955275A (en) 1997-02-14 1997-03-04 Methods for identifying nucleic acid sequences encoding agents that affect cellular phenotypes
PCT/US1998/004376 WO1998039483A1 (en) 1997-03-04 1998-02-27 Methods for identifying nucleic acid sequences encoding agents that affect cellular phenotypes

Publications (1)

Publication Number Publication Date
ATE224457T1 true ATE224457T1 (de) 2002-10-15

Family

ID=25211168

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98911497T ATE224457T1 (de) 1997-03-04 1998-02-27 Verfahren zur identifizierung von nukleinsäuren, welche agenzien kodieren, die zelluläre phenotypen beeinflussen

Country Status (11)

Country Link
US (3) US5955275A (de)
EP (1) EP0973943B1 (de)
JP (1) JP2001514510A (de)
AT (1) ATE224457T1 (de)
AU (1) AU745827B2 (de)
CA (1) CA2283261C (de)
DE (1) DE69808060T2 (de)
DK (1) DK0973943T3 (de)
IL (1) IL131583A0 (de)
NO (1) NO994290L (de)
WO (1) WO1998039483A1 (de)

Families Citing this family (84)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6365344B1 (en) * 1996-01-23 2002-04-02 The Board Of Trustees Of The Leland Stanford Junior University Methods for screening for transdominant effector peptides and RNA molecules
DE69722176T2 (de) * 1996-01-23 2004-03-18 The Board Of Trustees Of The Leland Stanford Junior University, Palo Alto Verfahren zur suche nach transdominanten effektorpeptiden und rna-molekülen
US5955275A (en) * 1997-02-14 1999-09-21 Arcaris, Inc. Methods for identifying nucleic acid sequences encoding agents that affect cellular phenotypes
US20040219569A1 (en) * 1999-07-06 2004-11-04 Fruma Yehiely Gene identification method
US20020081688A1 (en) * 1997-03-04 2002-06-27 Kamb Carl Alexander Retinoid pathway assays, and compositions therefrom
US20020086386A1 (en) * 1997-03-04 2002-07-04 Kamb Carl Alexander B-catenin assays, and compositions therefrom
JPH10260223A (ja) * 1997-03-19 1998-09-29 Fujitsu Ltd 半導体検査装置及びこれを用いた検査方法
US6451527B1 (en) 1997-08-29 2002-09-17 Selective Genetics, Inc. Methods using genetic package display for selecting internalizing ligands for gene delivery
US6589730B1 (en) * 1997-08-29 2003-07-08 Selective Genetics, Inc. Methods for identifying protein-protein interactions by selective transduction
RU2234530C2 (ru) * 1997-08-29 2004-08-20 Селектив Дженетикс, Инк. Способ идентификации бактериофага, способ выделения клеток млекопитающего, способ селекции бактериофага (варианты), способ идентификации подгруппы бактериофагов и интернализующий лиганд (варианты)
US7037648B1 (en) * 1997-11-07 2006-05-02 John Hopkins University Somatic transfer of modified genes to predict drug effects
AU1582799A (en) * 1997-11-07 1999-05-31 Iconix Pharmaceuticals, Inc. Surrogate genetics target characterization method
US6228579B1 (en) * 1997-11-14 2001-05-08 San Diego State University Foundation Method for identifying microbial proliferation genes
GB9726431D0 (en) * 1997-12-15 1998-02-11 Dower Steven Expression cloning and single cell detection of phenotype
US6897031B1 (en) * 1998-04-17 2005-05-24 Rigel Pharmaceuticals, Inc. Multiparameter FACS assays to detect alterations in exocytosis
US8334238B2 (en) * 1998-04-17 2012-12-18 Rigel, Inc. Multiparameter FACS assays to detect alterations in cellular parameters and to screen small molecule libraries
US6472151B1 (en) 1998-08-19 2002-10-29 Bristol-Myers Squibb Company Method of screening for compounds that modulate the activity of a molecular target
US6180343B1 (en) 1998-10-08 2001-01-30 Rigel Pharmaceuticals, Inc. Green fluorescent protein fusions with random peptides
US6936421B2 (en) 1998-10-08 2005-08-30 Rigel Pharmaceuticals, Inc. Structurally biased random peptide libraries based on different scaffolds
US7297482B2 (en) 1998-10-08 2007-11-20 Rigel Pharmaceuticals, Inc. Structurally biased random peptide libraries based on different scaffolds
AU1822800A (en) * 1998-11-17 2000-06-05 Deltagen Proteomics, Inc. Methods for validating polypeptide targets that correlate to cellular phenotypes
AU2596800A (en) * 1998-12-31 2000-07-31 Iconix Pharmaceuticals, Inc. Method for generating a pathway reporter system
US6720139B1 (en) 1999-01-27 2004-04-13 Elitra Pharmaceuticals, Inc. Genes identified as required for proliferation in Escherichia coli
US6342356B1 (en) * 1999-03-12 2002-01-29 Gpc Biotech, Inc. Methods and regents for identifying synthetic genetic elements
US7803765B2 (en) * 1999-05-05 2010-09-28 Phylogica Limited Methods of constructing biodiverse gene fragment libraries and biological modulators isolated therefrom
ATE320486T1 (de) * 1999-05-05 2006-04-15 Phylogica Ltd Isolierung von biologischen modulatoren aus bibiotheken mit biologisch vielfältigen genfragmenten
US7270969B2 (en) * 1999-05-05 2007-09-18 Phylogica Limited Methods of constructing and screening diverse expression libraries
AU2041901A (en) 1999-11-09 2001-06-06 Elitra Pharmaceuticals, Inc. Genes essential for microbial proliferation and antisense thereto
CA2394805A1 (en) 1999-12-16 2001-06-21 Iconix Pharmaceuticals, Inc Random domain mapping
WO2001059071A2 (en) 2000-02-09 2001-08-16 Genvec, Inc. Methods of preparing and using a viral vector library
US6582899B1 (en) * 2000-02-15 2003-06-24 Deltagen Proteomics, Inc. Methods for identifying agents that cause a lethal phenotype, and agents thereof
EP1728874B1 (de) * 2000-03-24 2012-09-26 Amgen Research (Munich) GmbH Verfahren zur Herstellung eines in vitro Surrogats zur Erkennung von in einer Probe exprimierten Genen
WO2001075178A2 (en) * 2000-04-04 2001-10-11 Enanta Pharmaceuticals, Inc. Methods for identifying peptide aptamers capable of altering a cell phenotype
JP2003534806A (ja) * 2000-05-31 2003-11-25 ジェンベク、インコーポレイティッド アデノウイルスベクターのターゲティングの方法および組成物
US6410246B1 (en) 2000-06-23 2002-06-25 Genetastix Corporation Highly diverse library of yeast expression vectors
US6406863B1 (en) * 2000-06-23 2002-06-18 Genetastix Corporation High throughput generation and screening of fully human antibody repertoire in yeast
US6410271B1 (en) 2000-06-23 2002-06-25 Genetastix Corporation Generation of highly diverse library of expression vectors via homologous recombination in yeast
CA2429515A1 (en) * 2000-11-17 2002-05-23 Deltagen Proteomics, Inc. Retinoid pathway assays, and compositions therefrom
AU2002236451A1 (en) * 2000-11-27 2002-07-24 Deltagen Proteomics Inc. Human rhinovirus assays, and compositions comprising anti-rhinoviral perturbagens
WO2002063032A1 (en) * 2001-02-05 2002-08-15 Shen Quan Pan Methods and kits for identifying scavengers of reactive oxygen species (ros)
WO2002070650A2 (en) * 2001-02-13 2002-09-12 Massachusetts Institute Of Technology Dynamic whole genome screening methodology and systems
JP2004530117A (ja) * 2001-02-22 2004-09-30 プラエシス ファーマシューティカルズ インコーポレーテッド 生物プロセスを調節するペプチドを同定する方法
CA2439263C (en) 2001-03-02 2012-10-23 Frank Becker Three hybrid assay system
US20030170642A1 (en) * 2001-03-12 2003-09-11 Irm, Llc Identification of cellular targets for biologically active molecules
AU2002252370A1 (en) * 2001-03-12 2002-09-24 Irm, Llc. Genomics-driven high speed cellular assays, development thereof, and collections of cellular reporters
US20020142337A1 (en) * 2001-03-29 2002-10-03 Edwards David N. Hybrid gene libraries and uses thereof
US7026123B1 (en) 2001-08-29 2006-04-11 Pioneer Hi-Bred International, Inc. UTR tag assay for gene function discovery
US20030134329A1 (en) * 2001-10-09 2003-07-17 Thea Norman Cross-species bioactive peptides
US20040029129A1 (en) * 2001-10-25 2004-02-12 Liangsu Wang Identification of essential genes in microorganisms
US20030170694A1 (en) * 2001-12-21 2003-09-11 Daniel Wall Stabilized nucleic acids in gene and drug discovery and methods of use
US20030143547A1 (en) * 2002-01-24 2003-07-31 Xianqiang Li Method for identifying multiple activated transcription factors
US20030219723A1 (en) * 2002-05-20 2003-11-27 Lu Henry H. Compositions and methods for screening and identifying anti-HCV agents
US20040067532A1 (en) 2002-08-12 2004-04-08 Genetastix Corporation High throughput generation and affinity maturation of humanized antibody
WO2005044978A2 (en) * 2003-07-15 2005-05-19 Washington University Methods of treating, preventing and inhibiting cancer metastasis and tumor formation
ITRM20030376A1 (it) 2003-07-31 2005-02-01 Univ Roma Procedimento per l'isolamento e l'espansione di cellule staminali cardiache da biopsia.
US7388976B2 (en) * 2004-03-09 2008-06-17 Siemens Medical Solutions Usa, Inc. Time-based system to link periodic X-ray images
EP1754052B1 (de) * 2004-06-03 2015-07-22 Phylogica Limited Modulatoren biochemischer eigenschaften
US11660317B2 (en) 2004-11-08 2023-05-30 The Johns Hopkins University Compositions comprising cardiosphere-derived cells for use in cell therapy
CA2638912A1 (en) * 2006-02-20 2007-08-30 Phylogica Limited Method of constructing and screening libraries of peptide structures
US8063012B2 (en) * 2006-09-19 2011-11-22 Phylogica Limited Neuroprotective peptide inhibitors of AP-1 signaling and uses therefor
KR101240487B1 (ko) * 2006-11-09 2013-03-08 더 존스 홉킨스 유니버시티 성체 포유동물 심근세포의 심장 줄기 세포로의 역분화
WO2008154700A1 (en) * 2007-06-20 2008-12-24 Phylogica Limited Compositions and uses thereof for the treatment of acute respiratory distress syndrome (ards) and clinical disorders associated with therewith
US7550323B2 (en) * 2007-08-08 2009-06-23 International Business Machines Corporation Electrical fuse with a thinned fuselink middle portion
US20090040006A1 (en) * 2007-08-08 2009-02-12 International Business Machines Corporation Electrical fuse with enhanced programming current divergence
JP5933894B2 (ja) 2007-09-14 2016-06-15 アディマブ， エルエルシー 合理的に設計された、合成抗体ライブラリおよびその使用
US12529164B2 (en) 2007-09-14 2026-01-20 Adimab, Llc Rationally designed, synthetic antibody libraries and uses therefor
US8877688B2 (en) 2007-09-14 2014-11-04 Adimab, Llc Rationally designed, synthetic antibody libraries and uses therefor
US7838963B2 (en) * 2007-10-26 2010-11-23 International Business Machines Corporation Electrical fuse having a fully silicided fuselink and enhanced flux divergence
CA2760153A1 (en) * 2009-04-27 2010-11-11 Alex Chenchik Reagents and methods for producing bioactive secreted peptides
US20130035255A1 (en) 2010-03-26 2013-02-07 Integratech Proteomics, Llc Controlled release hybrid systems
US9845457B2 (en) 2010-04-30 2017-12-19 Cedars-Sinai Medical Center Maintenance of genomic stability in cultured stem cells
US9249392B2 (en) 2010-04-30 2016-02-02 Cedars-Sinai Medical Center Methods and compositions for maintaining genomic stability in cultured stem cells
KR102810907B1 (ko) 2010-07-16 2025-05-21 아디맵 엘엘씨 항체 라이브러리
US9884076B2 (en) 2012-06-05 2018-02-06 Capricor, Inc. Optimized methods for generation of cardiac stem cells from cardiac tissue and their use in cardiac therapy
AU2013302799B2 (en) 2012-08-13 2018-03-01 Cedars-Sinai Medical Center Exosomes and micro-ribonucleic acids for tissue regeneration
WO2016054591A1 (en) 2014-10-03 2016-04-07 Cedars-Sinai Medical Center Cardiosphere-derived cells and exosomes secreted by such cells in the treatment of muscular dystrophy
AU2016353453B2 (en) 2015-11-11 2022-08-11 Serimmune Inc. Methods and compositions for assessing antibody specificities
US11253551B2 (en) 2016-01-11 2022-02-22 Cedars-Sinai Medical Center Cardiosphere-derived cells and exosomes secreted by such cells in the treatment of heart failure with preserved ejection fraction
WO2017210652A1 (en) 2016-06-03 2017-12-07 Cedars-Sinai Medical Center Cdc-derived exosomes for treatment of ventricular tachyarrythmias
EP3515459A4 (de) 2016-09-20 2020-08-05 Cedars-Sinai Medical Center Kardiosphärenzellen und deren extrazelluläre vesikel zur verzögerung oder umkehr des alterungsprozesses und altersbedingten erkrankungen
US11043823B2 (en) * 2017-04-06 2021-06-22 Tesla, Inc. System and method for facilitating conditioning and testing of rechargeable battery cells
CA3059910A1 (en) 2017-04-19 2018-10-25 Cedars-Sinai Medical Center Methods and compositions for treating skeletal muscular dystrophy
WO2019126068A1 (en) 2017-12-20 2019-06-27 Cedars-Sinai Medical Center Engineered extracellular vesicles for enhanced tissue delivery
WO2019152549A1 (en) 2018-02-05 2019-08-08 Cedars-Sinai Medical Center Methods for therapeutic use of exosomes and y-rnas

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5135854A (en) * 1987-10-29 1992-08-04 Zymogenetics, Inc. Methods of regulating protein glycosylation
US5283173A (en) * 1990-01-24 1994-02-01 The Research Foundation Of State University Of New York System to detect protein-protein interactions
US5364783A (en) * 1990-05-14 1994-11-15 Massachusetts Institute Of Technology Retrovirus promoter-trap vectors
US5401629A (en) 1990-08-07 1995-03-28 The Salk Institute Biotechnology/Industrial Associates, Inc. Assay methods and compositions useful for measuring the transduction of an intracellular signal
US5217889A (en) * 1990-10-19 1993-06-08 Roninson Igor B Methods and applications for efficient genetic suppressor elements
AU2808392A (en) * 1991-10-30 1993-06-07 General Hospital Corporation, The C-myc dna binding partners, motifs, screening assays, and uses thereof
GB9123987D0 (en) 1991-11-12 1992-01-02 Primm Srl New reporter genes
US5491084A (en) * 1993-09-10 1996-02-13 The Trustees Of Columbia University In The City Of New York Uses of green-fluorescent protein
WO1995016694A1 (en) * 1993-12-16 1995-06-22 Cold Spring Harbor Laboratory Origin of replication complex genes, proteins and methods
JP3468523B2 (ja) * 1994-01-21 2003-11-17 イコス コーポレイション ガゼインキナーゼ▲ｉ▼と相互作用するタンパク質に関連する方法および物質
US6117679A (en) * 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5691137A (en) * 1994-08-30 1997-11-25 Brandeis University Methods of screening candidate agents for biological activity using yeast cells
US5569588A (en) * 1995-08-09 1996-10-29 The Regents Of The University Of California Methods for drug screening
DE69722176T2 (de) 1996-01-23 2004-03-18 The Board Of Trustees Of The Leland Stanford Junior University, Palo Alto Verfahren zur suche nach transdominanten effektorpeptiden und rna-molekülen
US5945276A (en) 1996-04-10 1999-08-31 Signal Pharmaceuticals, Inc. Reporter cell line system for detecting cytomegalovirus and identifying modulators of viral gene expression
US5783431A (en) * 1996-04-24 1998-07-21 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
US6004808A (en) * 1996-06-21 1999-12-21 Aurora Biosciences Corporation Promiscuous G-protein compositions and their use
US5955275A (en) * 1997-02-14 1999-09-21 Arcaris, Inc. Methods for identifying nucleic acid sequences encoding agents that affect cellular phenotypes
US5998136A (en) * 1996-08-19 1999-12-07 Arcaris, Inc. Selection systems and methods for identifying genes and gene products involved in cell proliferation
US5928888A (en) 1996-09-26 1999-07-27 Aurora Biosciences Corporation Methods and compositions for sensitive and rapid, functional identification of genomic polynucleotides and secondary screening capabilities

Also Published As

Publication number Publication date
EP0973943B1 (de) 2002-09-18
DK0973943T3 (da) 2003-01-27
DE69808060T2 (de) 2003-04-30
CA2283261A1 (en) 1998-09-11
IL131583A0 (en) 2001-01-28
DE69808060D1 (de) 2002-10-24
NO994290L (no) 1999-11-03
US20020098503A1 (en) 2002-07-25
US20020018992A1 (en) 2002-02-14
AU745827B2 (en) 2002-04-11
NO994290D0 (no) 1999-09-03
AU6543898A (en) 1998-09-22
WO1998039483A1 (en) 1998-09-11
US5955275A (en) 1999-09-21
JP2001514510A (ja) 2001-09-11
CA2283261C (en) 2002-07-02
EP0973943A1 (de) 2000-01-26
US6579675B2 (en) 2003-06-17

Similar Documents

Publication Publication Date Title
ATE224457T1 (de) Verfahren zur identifizierung von nukleinsäuren, welche agenzien kodieren, die zelluläre phenotypen beeinflussen
Li et al. LINE-1 transcription activates long-range gene expression
Asp et al. Genome-wide remodeling of the epigenetic landscape during myogenic differentiation
Roychoudhury et al. Endogenous oxidized DNA bases and APE1 regulate the formation of G-quadruplex structures in the genome
Chaudhari et al. Evaluation of homology-independent CRISPR-Cas9 off-target assessment methods
Matsuoka et al. ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage
Oosterhof et al. Nucleas action on chromatin: evidence for discrete, repeated nucleoprotein units along chromatin fibrils.
DE69607830D1 (de) Verfahren zur isothermer amplifikation von nukleinsäure molekülen
ATE332968T1 (de) Erstellung und durchmusterung von interessierenden dna-banken in zellen von filamentösen pilzen
DE602004028280D1 (de) Verfahren zur induktion der entwicklung und terminalen differenzierung von gedächtnis-b-zellen
DE59804008D1 (de) Verfahren zur identifikation von nucleinsäuren durch matrix-assistierte laser desorptions/ionisations massenspektrometrie
ATE211506T1 (de) Ein verfahren zum nachweis biologisch aktiver substanzen
ATE397089T1 (de) Verfahren zum screenen von mehrfach transformierten zellen unter verwendung bicistronischer expression von fluoreszenzproteinen
Liu et al. Expression of TNRC6 (GW182) proteins is not necessary for gene silencing by fully complementary RNA duplexes
Spadafora Transgenerational epigenetic reprogramming of early embryos: a mechanistic model
Dubocanin et al. Single-molecule architecture and heterogeneity of human telomeric DNA and chromatin
DE69634563D1 (de) Verbesserte verfahren zur transfektion der t-zellen
DE59408917D1 (de) Verfahren zur identifizierung von menschlichen und tierischen zellen mit der fähigkeit zu unbegrenzter proliferation oder zur tumorbildung
ATE280838T1 (de) Verfahren zur identifikation von nucleinsäuren durch elektrospray massenspektrometrie
Ager et al. Analysis of restriction enzyme-induced DNA double-strand breaks in Chinese hamster ovary cells by pulsed-field gel electrophoresis: implications for chromosome damage
Looney et al. Systematic mapping of occluded genes by cell fusion reveals prevalence and stability of cis-mediated silencing in somatic cells
Maria et al. Expanding the list of sequence-agnostic enzymes for chromatin conformation capture assays with S1 nuclease
EP0846263A4 (de) Verfahren und testsatz zur evaluierung der metastaseneigung von tumoren
BR9708256A (pt) Processo para identificar genes com expresão específica ao síto ou de síto preferido em células de marcacção específicas presentes em um ambiente de células diferente ou não daquele de sua origem e uso do mesmo
Vergara et al. Megabase-scale detachment of genome-nuclear lamina interactions is required for efficient repair of double stranded breaks

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 0973943

Country of ref document: EP

REN Ceased due to non-payment of the annual fee