ATE226252T1 - Subtilinvarianten mit erhöhter stabilität und aktivität - Google Patents

Subtilinvarianten mit erhöhter stabilität und aktivität

Info

Publication number: ATE226252T1
Authority: AT; Austria
Prior art keywords: mutant; polypeptides; induce; gene; exhibits
Prior art date: 1992-11-25

Application number

AT94902206T

Other languages

English (en)

Inventor

J Norman Hansen

Original Assignee

Univ Maryland

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1992-11-25

Filing date

1993-11-08

Publication date

2002-11-15

1993-11-08 Application filed by Univ Maryland filed Critical Univ Maryland

2002-11-15 Application granted granted Critical

2002-11-15 Publication of ATE226252T1 publication Critical patent/ATE226252T1/de

Links

230000000694 effects Effects 0.000 title abstract 2
238000000034 method Methods 0.000 abstract 2
229920001184 polypeptide Polymers 0.000 abstract 2
108090000765 processed proteins & peptides Proteins 0.000 abstract 2
102000004196 processed proteins & peptides Human genes 0.000 abstract 2
108090000623 proteins and genes Proteins 0.000 abstract 2
241000894006 Bacteria Species 0.000 abstract 1
108010062877 Bacteriocins Proteins 0.000 abstract 1
UQBOJOOOTLPNST-UHFFFAOYSA-N Dehydroalanine Chemical group NC(=C)C(O)=O UQBOJOOOTLPNST-UHFFFAOYSA-N 0.000 abstract 1
108010076504 Protein Sorting Signals Proteins 0.000 abstract 1
229940024606 amino acid Drugs 0.000 abstract 1
150000001413 amino acids Chemical class 0.000 abstract 1
210000004666 bacterial spore Anatomy 0.000 abstract 1
RKLXDNHNLPUQRB-TVJUEJKUSA-N chembl564271 Chemical compound C([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]1C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]2C(C)SC[C@H](N[C@@H](CC(N)=O)C(=O)NC(=O)[C@@H](NC2=O)CSC1C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)NC(=C)C(=O)N[C@@H](CCCCN)C(O)=O)NC(=O)[C@H]1NC(=O)C(=C\C)/NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H]2NC(=O)CNC(=O)[C@@H]3CCCN3C(=O)[C@@H](NC(=O)[C@H]3N[C@@H](CC(C)C)C(=O)NC(=O)C(=C)NC(=O)CC[C@H](NC(=O)[C@H](NC(=O)[C@H](CCCCN)NC(=O)[C@@H](N)CC=4C5=CC=CC=C5NC=4)CSC3)C(O)=O)C(C)SC2)C(C)C)C(C)SC1)C1=CC=CC=C1 RKLXDNHNLPUQRB-TVJUEJKUSA-N 0.000 abstract 1
229960000310 isoleucine Drugs 0.000 abstract 1
AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 abstract 1
125000000741 isoleucyl group Chemical group [H]N([H])C(C(C([H])([H])[H])C([H])([H])C([H])([H])[H])C(=O)O* 0.000 abstract 1
230000004048 modification Effects 0.000 abstract 1
238000012986 modification Methods 0.000 abstract 1
239000013612 plasmid Substances 0.000 abstract 1
230000004481 post-translational protein modification Effects 0.000 abstract 1
238000002741 site-directed mutagenesis Methods 0.000 abstract 1
108010082567 subtilin Proteins 0.000 abstract 1
230000001629 suppression Effects 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/90—Fusion polypeptide containing a motif for post-translational modification

Landscapes

Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Engineering & Computer Science (AREA)
Organic Chemistry (AREA)
Biomedical Technology (AREA)
Wood Science & Technology (AREA)
Biotechnology (AREA)
Molecular Biology (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
Zoology (AREA)
General Health & Medical Sciences (AREA)
Biophysics (AREA)
Biochemistry (AREA)
Plant Pathology (AREA)
Physics & Mathematics (AREA)
Microbiology (AREA)
Medicinal Chemistry (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Gastroenterology & Hepatology (AREA)
Crystallography & Structural Chemistry (AREA)
Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
Public Health (AREA)
Chemical Kinetics & Catalysis (AREA)
General Chemical & Material Sciences (AREA)
Communicable Diseases (AREA)
Pharmacology & Pharmacy (AREA)
Animal Behavior & Ethology (AREA)
Oncology (AREA)
Veterinary Medicine (AREA)
Peptides Or Proteins (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Saccharide Compounds (AREA)
Medicines Containing Material From Animals Or Micro-Organisms (AREA)

AT94902206T 1992-11-25 1993-11-08 Subtilinvarianten mit erhöhter stabilität und aktivität ATE226252T1 (de)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US07/981,525 US5516682A (en)	1988-07-05	1992-11-25	Subtilin variant of enhanced stability and activity
PCT/US1993/010558 WO1994012528A1 (en)	1992-11-25	1993-11-08	Subtilin variant of enhanced stability and activity

Publications (1)

Publication Number	Publication Date
ATE226252T1 true ATE226252T1 (de)	2002-11-15

Family

ID=25528432

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT94902206T ATE226252T1 (de)	1992-11-25	1993-11-08	Subtilinvarianten mit erhöhter stabilität und aktivität

Country Status (8)

Country	Link
US (4)	US5516682A (de)
EP (1)	EP0670847B1 (de)
JP (1)	JP3447057B2 (de)
AT (1)	ATE226252T1 (de)
AU (1)	AU687369B2 (de)
CA (1)	CA2150025A1 (de)
DE (1)	DE69332411T2 (de)
WO (1)	WO1994012528A1 (de)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5516682A (en) *	1988-07-05	1996-05-14	University Of Maryland	Subtilin variant of enhanced stability and activity
US5861275A (en) *	1995-09-28	1999-01-19	The University Of Maryland	Lantibiotic mutants and chimeras of enhanced stability and activity, leader sequences therefor, genes encoding the same, and methods of producing and using the same
US5919695A (en) *	1997-04-22	1999-07-06	Quest International, B.V.	Method and culture for inhibiting undesired microorganisms with culture of Bacillus subtilis
US6953683B2 (en) *	1997-07-18	2005-10-11	University Of Maryland	Compositions and methods of inhibiting bacterial spore germination
WO1999003352A1 (en) *	1997-07-18	1999-01-28	University Of Maryland	Sublancin lantibiotic produced by bacillus subtilis 168
CA2356955A1 (en) *	1998-12-24	2000-07-06	University Of Maryland	Stable subtilin and methods of manufacture
US6846804B2 (en) *	2000-06-29	2005-01-25	University Of Maryland, College Park Office Of Technology Commercialization	Construction of a structural variant of sublancin to facilitate its isolation and use in bioremediation of environmental contamination by gram-positive spore formers such as bacillus anthrasis
US6759205B2 (en)	2000-06-29	2004-07-06	University Of Maryland	Construction of a strain of Bacillus subtilis 168 that displays the sublancin lantibiotic on the surface of the cell
US7033766B2 (en) *	2000-06-29	2006-04-25	University Of Maryland Office Of Technology Commercialization	Construction and screening of lantibody display libraries
US20050026270A1 (en) *	2001-06-20	2005-02-03	University Of Maryland	Stable subtilin and methods of manufacture
US6861236B2 (en) *	2002-05-24	2005-03-01	Applied Nanosystems B.V.	Export and modification of (poly)peptides in the lantibiotic way
US7153666B2 (en) *	2003-07-17	2006-12-26	General Atomics	Methods and compositions for determination of glycated proteins
US8187831B2 (en) *	2003-09-19	2012-05-29	General Atomics	Determination of ions using ion-sensitive enzymes
US7022494B2 (en) *	2003-09-19	2006-04-04	General Atomics	Detection of potassium ions using ion-sensitive enzymes
JP2009544315A (ja) *	2006-07-25	2009-12-17	ジェネラルアトミクス	糖化ヘモグロビンのパーセントを定量するための方法
US7943385B2 (en)	2006-07-25	2011-05-17	General Atomics	Methods for assaying percentage of glycated hemoglobin
CN102076867A (zh) *	2008-05-13	2011-05-25	通用原子公司	用于直接测定糖化血红蛋白百分比的电化学生物传感器
CA2865395A1 (en)	2012-02-27	2013-09-06	Oragenics, Inc.	Variants of the lantibiotic mu1140 and other lantibiotics with improved pharmacological properties and structural features
CN113039196B (zh) *	2018-10-31	2025-03-18	株式会社村田制作所	抗革兰氏阴性菌化合物
EP3935067A4 (de) *	2019-03-08	2022-12-07	Fraunhofer USA, Inc.	Neuartiges antimikrobielles lantibiotisches peptid und verwendungen davon

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5516682A (en) *	1988-07-05	1996-05-14	University Of Maryland	Subtilin variant of enhanced stability and activity
US5218101A (en) *	1988-07-05	1993-06-08	The University Of Maryland	Leader sequence inducing a post-translational modification of polypeptides in bacteria, and gene therefor
IE892893A1 (en) *	1990-03-11	1991-09-25	Charles O Kane	Phage-like bacteriocins
DE69232930T2 (de) *	1991-04-11	2003-12-11	Stichting Nederlands Instituut Voor Zuivelonderzoek (Nizo), Ede	Nisin a aehnliche lantibiotika, diese produzierende milchsaeurebakterien und deren herstellung

1992
- 1992-11-25 US US07/981,525 patent/US5516682A/en not_active Expired - Fee Related
1993
- 1993-11-08 WO PCT/US1993/010558 patent/WO1994012528A1/en not_active Ceased
- 1993-11-08 EP EP94902206A patent/EP0670847B1/de not_active Expired - Lifetime
- 1993-11-08 DE DE69332411T patent/DE69332411T2/de not_active Expired - Fee Related
- 1993-11-08 AT AT94902206T patent/ATE226252T1/de not_active IP Right Cessation
- 1993-11-08 JP JP51314594A patent/JP3447057B2/ja not_active Expired - Fee Related
- 1993-11-08 CA CA002150025A patent/CA2150025A1/en not_active Abandoned
- 1993-11-08 AU AU56658/94A patent/AU687369B2/en not_active Ceased
1994
- 1994-03-30 US US08/220,033 patent/US5576420A/en not_active Expired - Lifetime
1995
- 1995-06-05 US US08/465,491 patent/US5885811A/en not_active Expired - Lifetime
1997
- 1997-12-08 US US08/986,617 patent/US5914250A/en not_active Expired - Fee Related

Also Published As

Publication number	Publication date
CA2150025A1 (en)	1994-06-09
DE69332411D1 (de)	2002-11-21
DE69332411T2 (de)	2003-06-26
US5516682A (en)	1996-05-14
EP0670847A1 (de)	1995-09-13
AU5665894A (en)	1994-06-22
JP3447057B2 (ja)	2003-09-16
EP0670847B1 (de)	2002-10-16
US5885811A (en)	1999-03-23
JPH08506010A (ja)	1996-07-02
WO1994012528A1 (en)	1994-06-09
AU687369B2 (en)	1998-02-26
US5914250A (en)	1999-06-22
US5576420A (en)	1996-11-19
EP0670847A4 (de)	1997-06-11

Legal Events

Date	Code	Title	Description
2003-04-15	RER	Ceased as to paragraph 5 lit. 3 law introducing patent treaties

Publication	Publication Date	Title
ATE226252T1 (de)	2002-11-15	Subtilinvarianten mit erhöhter stabilität und aktivität
DE3378830D1 (en)	1989-02-09	Synthetic heat-stable enterotoxin polypeptide and multimers thereof
PL2251353T3 (pl)	2013-08-30	Homogenne preparaty IL-29
IL77081A0 (en)	1986-04-29	Cloned human erythropoietin genes and products thereof
WO1994012638A3 (en)	1994-09-01	Interleukin-3 (il-3) multiple mutation polypeptides
ES8605040A1 (es)	1986-02-16	Procedimiento para preparar un polipeptido con actividad de hirudina
IL112403A0 (en)	1995-03-30	Method for producing a heterologous polypeptide
EP0423224A4 (en)	1991-11-06	Leader sequence inducing a post-translational modification of polypeptides in bacteria, and gene therefor
ATE176500T1 (de)	1999-02-15	Sekretionsvektor, diesen enthaltene transformierte mikroorganismen und herstellung von produkten durch obigen mikroorganismus
ATE140008T1 (de)	1996-07-15	Polypeptid
AU1847092A (en)	1993-01-07	Implant compositions containing a biologically active protein, peptide or polypeptide
AU637589B2 (en)	1993-06-03	Ancrod proteins, their production and use
EP0750040A3 (de)	1999-10-27	Varianten des Makrophagen-stimulierendes-Proteins und Verfahren zur ihrer Herstellung
EP0352707A3 (de)	1991-09-11	Expressionssystem für die Sekretion von bioaktivem, menschlichem granulozyt-makrophagem Koloniestimulierungsfaktor (GM-CSF) und anderen heterologen Proteinen aus Streptomyces
KR910700345A (ko)	1991-03-14	사람 갑상선 기원의 재조합 C-말단 α-아미드화 효소
CA2083286A1 (en)	1992-01-27	Recombinant fibrinogenases, preparation and use thereof
EP1295943A4 (de)	2005-11-16	Polypeptide mit aktivität der stimulierung von neutrophilen
DE69121227D1 (de)	1996-09-12	Polypeptid