ATE176500T1 - Sekretionsvektor, diesen enthaltene transformierte mikroorganismen und herstellung von produkten durch obigen mikroorganismus - Google Patents

Sekretionsvektor, diesen enthaltene transformierte mikroorganismen und herstellung von produkten durch obigen mikroorganismus

Info

Publication number: ATE176500T1
Authority: AT; Austria
Prior art keywords: hirudin; production; products; transformed microorganisms; secretion vector
Prior art date: 1990-11-08

Application number

AT95202092T

Other languages

English (en)

Inventor

Satoru Misawa

Yoshifumi Inoue

Hitoshi Matsuda

Hideyuki Furuya

Original Assignee

Japan Energy Corp

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1990-11-08

Filing date

1991-11-08

Publication date

1999-02-15

1990-11-08 Priority claimed from JP2303096A external-priority patent/JPH07119237B2/ja

1991-02-13 Priority claimed from JP4127191A external-priority patent/JP3226289B2/ja

1991-11-08 Application filed by Japan Energy Corp filed Critical Japan Energy Corp

1999-02-15 Application granted granted Critical

1999-02-15 Publication of ATE176500T1 publication Critical patent/ATE176500T1/de

Links

244000005700 microbiome Species 0.000 title abstract 3
239000013606 secretion vector Substances 0.000 title abstract 3
WQPDUTSPKFMPDP-OUMQNGNKSA-N hirudin Chemical class C([C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1C=CC(OS(O)(=O)=O)=CC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CCCCN)NC(=O)[C@H]1N(CCC1)C(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H]1NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)CNC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)CC)NC(=O)[C@@H]2CSSC[C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@H](C(=O)N[C@H](C(NCC(=O)N[C@@H](CCC(N)=O)C(=O)NCC(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCCN)C(=O)N2)=O)CSSC1)C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]1NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)CNC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=2C=CC(O)=CC=2)NC(=O)[C@@H](NC(=O)[C@@H](N)C(C)C)C(C)C)[C@@H](C)O)CSSC1)C(C)C)[C@@H](C)O)[C@@H](C)O)C1=CC=CC=C1 WQPDUTSPKFMPDP-OUMQNGNKSA-N 0.000 abstract 3
108091028043 Nucleic acid sequence Proteins 0.000 abstract 2
241000588724 Escherichia coli Species 0.000 abstract 1
108010007267 Hirudins Proteins 0.000 abstract 1
102000007625 Hirudins Human genes 0.000 abstract 1
108010076504 Protein Sorting Signals Proteins 0.000 abstract 1
108020005091 Replication Origin Proteins 0.000 abstract 1
108010010967 hirudin HV1 Proteins 0.000 abstract 1
108010010961 hirudin HV3 Proteins 0.000 abstract 1
239000013612 plasmid Substances 0.000 abstract 1
108090000623 proteins and genes Proteins 0.000 abstract 1
102000004169 proteins and genes Human genes 0.000 abstract 1
230000014616 translation Effects 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/33—Fusion polypeptide fusions for targeting to specific cell types, e.g. tissue specific targeting, targeting of a bacterial subspecies

Landscapes

Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Genetics & Genomics (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Wood Science & Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
Zoology (AREA)
Biomedical Technology (AREA)
General Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Biotechnology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Microbiology (AREA)
Medicinal Chemistry (AREA)
Biophysics (AREA)
Crystallography & Structural Chemistry (AREA)
Plant Pathology (AREA)
Tropical Medicine & Parasitology (AREA)
Gastroenterology & Hepatology (AREA)
Physics & Mathematics (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Peptides Or Proteins (AREA)
Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Medicines Containing Plant Substances (AREA)

AT95202092T 1990-11-08 1991-11-08 Sekretionsvektor, diesen enthaltene transformierte mikroorganismen und herstellung von produkten durch obigen mikroorganismus ATE176500T1 (de)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
JP2303096A JPH07119237B2 (ja)	1990-11-08	1990-11-08	ヒルジン変異体，その製造法及び抗凝血剤
JP4127191A JP3226289B2 (ja)	1991-02-13	1991-02-13	分泌ベクター、該ベクターで形質転換した微生物及び該微生物から産生される産物の製造法

Publications (1)

Publication Number	Publication Date
ATE176500T1 true ATE176500T1 (de)	1999-02-15

Family

ID=26380836

Family Applications (2)

Application Number	Title	Priority Date	Filing Date
AT95202092T ATE176500T1 (de)	1990-11-08	1991-11-08	Sekretionsvektor, diesen enthaltene transformierte mikroorganismen und herstellung von produkten durch obigen mikroorganismus
AT91919149T ATE140929T1 (de)	1990-11-08	1991-11-08	Hirudinmutante, deren herstellung, antikoagulans, sekretorischer vektor, durch besagten vektor transformierter mikroorganismus und herstellung eines produkts durch besagten mikroorganismus

Family Applications After (1)

Application Number	Title	Priority Date	Filing Date
AT91919149T ATE140929T1 (de)	1990-11-08	1991-11-08	Hirudinmutante, deren herstellung, antikoagulans, sekretorischer vektor, durch besagten vektor transformierter mikroorganismus und herstellung eines produkts durch besagten mikroorganismus

Country Status (12)

Country	Link
US (2)	US5516656A (de)
EP (2)	EP0511393B1 (de)
AT (2)	ATE176500T1 (de)
AU (2)	AU648124B2 (de)
CA (2)	CA2072375C (de)
DE (2)	DE69130872T2 (de)
DK (2)	DK0687731T3 (de)
ES (2)	ES2129749T3 (de)
FI (1)	FI107928B (de)
GR (2)	GR3021410T3 (de)
NO (2)	NO303735B1 (de)
WO (1)	WO1992008736A1 (de)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO1985004418A1 (fr) *	1984-03-27	1985-10-10	Transgene S.A.	Vecteurs d'expression de l'hirudine, cellules transformees et procede de preparation de l'hirudine
DE4009268A1 (de) *	1990-03-22	1991-09-26	Consortium Elektrochem Ind	Sekretion von hirudinderivaten
US5972648A (en) *	1993-09-28	1999-10-26	Japan Energy Corporation	Hirudin analogs, methods of manufacture thereof and anticoagulant compositions having these as active ingredients
RU2182598C2 (ru) *	1994-11-21	2002-05-20	Новартис Аг	Модифицированные ингибиторы протеиназы
DE19944870A1 (de)	1999-09-18	2001-03-29	Aventis Pharma Gmbh	Signalsequenzen zur Herstellung von Leu-Hirudin über Sekretion durch E. coli in das Kulturmedium
DE10108211A1 (de) *	2001-02-20	2002-08-22	Aventis Pharma Gmbh	Verwendung von Fusionsproteinen, deren N-terminaler Anteil aus einem Hirudinderivat besteht, zur Herstellung rekombinanter Proteine über Sekretion durch Hefen
DE10108212A1 (de) *	2001-02-20	2002-08-22	Aventis Pharma Gmbh	Fusionsprotein zur Sekretion von Wertprotein in bakterielle Überstände
US7202059B2 (en)	2001-02-20	2007-04-10	Sanofi-Aventis Deutschland Gmbh	Fusion proteins capable of being secreted into a fermentation medium
US7638618B2 (en)	2001-02-20	2009-12-29	Sanofi-Aventis Deutschland Gmbh	Nucleic acids encoding a hirudin and pro-insulin as superscretable peptides and for parallel improvement of the exported forms of one or more polypeptides of interest
KR100447530B1 (ko) *	2001-08-14	2004-09-08	한국과학기술원	ＯｍｐＦ를 이용하여 목적 단백질을 대장균 세포외로분비생산하는 방법
EP1578946A4 (de)	2002-08-23	2009-08-26	Du Pont	Nutzung von stärkeprodukten zur biologischen produktion mittels fermentation
JP2006096668A (ja) *	2002-11-08	2006-04-13	Ono Pharmaceut Co Ltd	エラスターゼ阻害剤と血液凝固系および／または線溶系酵素阻害剤との組み合わせからなる医薬
US7795205B2 (en) *	2004-04-12	2010-09-14	Canyon Pharmaceuticals, Inc.	Methods for effecting regression of tumor mass and size in a metastasized pancreatic tumor
CN101094867B (zh)	2004-10-19	2011-08-24	隆萨股份公司	用于固相肽合成的方法
DK1903115T3 (da) *	2006-09-22	2011-05-23	Wacker Chemie Ag	Fremgangsmåde til fermentativ fremstilling af antistoffer
CN101372512B (zh) *	2007-08-23	2011-03-23	中国人民解放军军事医学科学院生物工程研究所	一类抗凝血多肽及其用途
WO2010054503A1 (zh) *	2008-11-17	2010-05-20	中国人民解放军军事医学科学院生物工程研究所	抗凝血多肽及其应用
CN115572329B (zh) *	2021-06-21	2024-02-06	王大勇	一组活性增强代谢较慢的菲牛蛭基因重组水蛭素及其制备方法

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
FR2562088B1 (fr) *	1984-03-27	1987-11-13	Transgene Sa	Vecteurs d'expression de l'hirudine, cellules transformees et procede de preparation de l'hirudine
DE3586386T2 (de) *	1984-10-05	1993-01-14	Genentech Inc	Dna, zellkulturen und verfahren zur sekretion von heterologen proteinen und periplasmische proteinrueckgewinnung.
DE3445532A1 (de) *	1984-12-13	1986-06-19	Plantorgan Werk Heinrich G.E. Christensen, KG, 2903 Bad Zwischenahn	Hirudin-pa, desulfatohirudine-pa, verfahren zur herstellung und pharmazeutische mittel, die diese wirkstoffe enthalten
DE3506992A1 (de) *	1985-02-27	1986-08-28	Plantorgan Werk Heinrich G.E. Christensen, KG, 2903 Bad Zwischenahn	Modifizierte hirudine, verfahren zu deren herstellung und pharmazeutische mittel, die diese wirkstoffe enthalten
FR2607517B2 (fr) *	1986-12-01	1989-12-22	Transgene Sa	Vecteurs d'expression de variants de l'hirudine dans les levures, procede et produit obtenu
US5084384A (en) *	1987-04-23	1992-01-28	Monsanto Company	Secretion of insulin-like growth factor-I
JP2518862B2 (ja) *	1987-08-31	1996-07-31	株式会社蛋白工学研究所	新規プラスミドベクタ―
AU604925B2 (en) *	1988-02-23	1991-01-03	Schering Aktiengesellschaft	A hirudin derivative
AU614121B2 (en)	1988-05-04	1991-08-22	Novartis Ag	Improvements in the production of polypeptides
DE58907266T2 (de) *	1988-06-11	1994-09-29	Ciba Geigy Ag	Polypeptide mit einer die Koagulierung hemmenden Wirkung.
US5268296A (en) *	1988-06-11	1993-12-07	Ciba-Geigy Corporation	DNA vector and recombinant host cell for production of hirullin P6 and P18
JPH0227993A (ja) *	1988-07-15	1990-01-30	Nippon Shinyaku Co Ltd	ｈＥＧＦの製法
JPH0648988B2 (ja)	1988-07-26	1994-06-29	工業技術院長	トロンビン阻害物質の製造法
US5223407A (en) *	1988-08-31	1993-06-29	Allelix Inc.	Excretion of heterologous proteins from e. coli
JPH0292288A (ja) *	1988-09-30	1990-04-03	Japan Tobacco Inc	アクアライシンｉ前駆体をコードする遺伝子、それを含む発現ベクター、該発現ベクターを含む大腸菌及びそれを用いたアクアライシンｉの製造方法
DE3835815A1 (de) *	1988-10-21	1990-04-26	Hoechst Ag	Neue isohirudine
JPH03164184A (ja)	1989-08-10	1991-07-16	Nippon Mining Co Ltd	ヒルジン発現ベクター、ヒルジン融合蛋白、形質転換微生物及びヒルジンの製造法
PT94949A (pt) *	1989-08-10	1991-05-22	Nippon Mining Co	Vector de expressao para hirudina, microorganismos transformados e metodos para a producao de hirudina
JPH0376579A (ja)	1989-08-18	1991-04-02	Nippon Mining Co Ltd	ヒト‐アンジオジェニン発現ベクター及びヒト‐アンジオジェニンの製造方法
GB9010552D0 (en) *	1990-05-10	1990-07-04	Erba Carlo Spa	Method for the recombinant production of hirudins and novel hirudins

1991
- 1991-11-08 ES ES95202092T patent/ES2129749T3/es not_active Expired - Lifetime
- 1991-11-08 CA CA002072375A patent/CA2072375C/en not_active Expired - Fee Related
- 1991-11-08 WO PCT/JP1991/001533 patent/WO1992008736A1/ja not_active Ceased
- 1991-11-08 AT AT95202092T patent/ATE176500T1/de not_active IP Right Cessation
- 1991-11-08 DE DE69130872T patent/DE69130872T2/de not_active Expired - Fee Related
- 1991-11-08 EP EP91919149A patent/EP0511393B1/de not_active Expired - Lifetime
- 1991-11-08 DK DK95202092T patent/DK0687731T3/da active
- 1991-11-08 CA CA002255396A patent/CA2255396A1/en not_active Abandoned
- 1991-11-08 AT AT91919149T patent/ATE140929T1/de not_active IP Right Cessation
- 1991-11-08 ES ES91919149T patent/ES2093717T3/es not_active Expired - Lifetime
- 1991-11-08 DE DE69121192T patent/DE69121192T2/de not_active Expired - Fee Related
- 1991-11-08 DK DK91919149.4T patent/DK0511393T3/da active
- 1991-11-08 EP EP95202092A patent/EP0687731B1/de not_active Expired - Lifetime
- 1991-11-08 AU AU88466/91A patent/AU648124B2/en not_active Ceased
1992
- 1992-06-26 FI FI922963A patent/FI107928B/fi active
- 1992-07-07 NO NO922671A patent/NO303735B1/no not_active IP Right Cessation
- 1992-07-08 US US07/910,528 patent/US5516656A/en not_active Expired - Fee Related
1994
- 1994-01-25 AU AU54701/94A patent/AU673870B2/en not_active Ceased
- 1994-11-28 US US08/348,972 patent/US5573929A/en not_active Expired - Fee Related
1996
- 1996-10-23 GR GR960402781T patent/GR3021410T3/el unknown
1998
- 1998-05-14 NO NO982207A patent/NO982207D0/no not_active Application Discontinuation
1999
- 1999-03-30 GR GR990400915T patent/GR3029824T3/el unknown

Also Published As

Publication number	Publication date
DE69121192D1 (de)	1996-09-05
CA2072375C (en)	2000-01-11
FI107928B (fi)	2001-10-31
EP0511393B1 (de)	1996-07-31
ES2129749T3 (es)	1999-06-16
DE69130872D1 (de)	1999-03-18
NO982207L (no)	1992-09-07
GR3021410T3 (en)	1997-01-31
US5516656A (en)	1996-05-14
EP0511393A4 (en)	1993-08-11
EP0687731B1 (de)	1999-02-03
AU673870B2 (en)	1996-11-28
AU5470194A (en)	1994-03-24
US5573929A (en)	1996-11-12
AU8846691A (en)	1992-06-11
ES2093717T3 (es)	1997-01-01
ATE140929T1 (de)	1996-08-15
EP0511393A1 (de)	1992-11-04
DE69121192T2 (de)	1997-02-20
DK0687731T3 (da)	1999-09-20
NO303735B1 (no)	1998-08-24
CA2255396A1 (en)	1992-05-09
AU648124B2 (en)	1994-04-14
FI922963A0 (fi)	1992-06-26
CA2072375A1 (en)	1992-05-09
NO922671L (no)	1992-09-07
FI922963L (fi)	1992-06-26
EP0687731A1 (de)	1995-12-20
NO922671D0 (no)	1992-07-07
NO982207D0 (no)	1998-05-14
WO1992008736A1 (en)	1992-05-29
DE69130872T2 (de)	1999-08-26
GR3029824T3 (en)	1999-06-30
DK0511393T3 (da)	1996-12-09

Legal Events

Date	Code	Title	Description
1999-09-15	UEP	Publication of translation of european patent specification
2004-08-15	REN	Ceased due to non-payment of the annual fee

Publication	Publication Date	Title
DE69130872D1 (de)	1999-03-18	Sekretionsvektor, diesen enthaltene transformierte Mikroorganismen und Herstellung von Produkten durch obigen Mikroorganismus
EP0048970A3 (de)	1982-08-18	Polypeptide, Verfahren zu ihrer mikrobischen Herstellung, Zwischenprodukte dafür und diese enthaltende Zusammensetzungen
AU1672388A (en)	1988-12-01	Expression of human proapolipoprotein a-i
CA2099841A1 (en)	1992-09-06	Expression of recombinant proteins in attenuated bacteria
CA2032153A1 (fr)	1990-10-29	Application de nouveaux fragments d'adn en tant que sequence codant pour un peptide signal pour la secretion de proteines matures par des levures recombinantes, cassettes d'expression, levures transformees et procede de preparation de proteines correspondant
HK49285A (en)	1985-07-05	Interferons and process for their preparation
DE3856265D1 (de)	1998-12-24	Varianten des pankreatischen Rinder-Trypsininhibitors, deren Herstellung und Verwendung
AU1003888A (en)	1988-09-01	Pancreatic secretory trypsin inhibitor and variants thereof produced by a recombinant host, process, expression vector and recombinant host therefore and pharmaceutical use thereof
NO911138L (no)	1991-09-23	Bakterielle vektorer.
DE3683267D1 (de)	1992-02-13	Gm-csf-protein, seine derivate, herstellung solcher proteine und ihre verwendung.
ATE226252T1 (de)	2002-11-15	Subtilinvarianten mit erhöhter stabilität und aktivität
ES2056798T3 (es)	1994-10-16	Procedimiento para la obtencion de proteinas por tecnologia genetica con utilizacion de celulas hospedantes gram-negativas.
HU895788D0 (en)	1991-08-28	Process for producing peptides and medical products containing them
ZA916171B (en)	1992-04-29	Protein structure of the plant toxin gelonin
TW224427B (de)	1994-06-01
ATE45184T1 (de)	1989-08-15	Vektor, teilweise oder voellig modifiziert mit dem lam b gen und mit diesem vektor modifizierte mikroorganismen befaehigt zum synthetisieren eines bestimmten externmembran-proteins, kodiert durch ein inserat ebenfalls enthalten im genannten vektor.
DK0387646T3 (da)	1996-09-16	Achromobacter-protease I-gen og genprodukt deraf
EP0422217A4 (en)	1992-08-19	Human recombinant placental ribonuclease inhibitor and method of production
ATE346941T1 (de)	2006-12-15	Protein-disulfidisomerase-gen eines methylotrophen hefestammes
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