ATE232908T1 - Verfahren zur enzymatischen sulfatierung mit hilfe von 3'-phosphoadenosin-5'phosphosulfat und wiederverwendbare phosphorylierte adenosin- zwischen-produkte - Google Patents

Verfahren zur enzymatischen sulfatierung mit hilfe von 3'-phosphoadenosin-5'phosphosulfat und wiederverwendbare phosphorylierte adenosin- zwischen-produkte

Info

Publication number
ATE232908T1
ATE232908T1 AT96908871T AT96908871T ATE232908T1 AT E232908 T1 ATE232908 T1 AT E232908T1 AT 96908871 T AT96908871 T AT 96908871T AT 96908871 T AT96908871 T AT 96908871T AT E232908 T1 ATE232908 T1 AT E232908T1
Authority
AT
Austria
Prior art keywords
enzymatic
phosphosulphate
sulfatation
phosphoadenosine
reusable
Prior art date
Application number
AT96908871T
Other languages
English (en)
Inventor
Chi-Huey Wong
Chun-Hung Lin
Gwo-Jenn Shen
Original Assignee
Scripps Research Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scripps Research Inst filed Critical Scripps Research Inst
Application granted granted Critical
Publication of ATE232908T1 publication Critical patent/ATE232908T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/13—Transferases (2.) transferring sulfur containing groups (2.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P11/00—Preparation of sulfur-containing organic compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
AT96908871T 1995-03-22 1996-03-21 Verfahren zur enzymatischen sulfatierung mit hilfe von 3'-phosphoadenosin-5'phosphosulfat und wiederverwendbare phosphorylierte adenosin- zwischen-produkte ATE232908T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/408,774 US5919673A (en) 1995-03-22 1995-03-22 One-pot enzymatic sulfation process using 3'-phosphoadenosine-5'-phosphosulfate and recycled phosphorylated adenosine intermediates
PCT/US1996/003792 WO1996029424A1 (en) 1995-03-22 1996-03-21 One-pot enzymatic sulfation process using 3'-phosphoadenosine-5'-phosphosulfate and recycled phosphorylated adenosine intermediates

Publications (1)

Publication Number Publication Date
ATE232908T1 true ATE232908T1 (de) 2003-03-15

Family

ID=23617712

Family Applications (1)

Application Number Title Priority Date Filing Date
AT96908871T ATE232908T1 (de) 1995-03-22 1996-03-21 Verfahren zur enzymatischen sulfatierung mit hilfe von 3'-phosphoadenosin-5'phosphosulfat und wiederverwendbare phosphorylierte adenosin- zwischen-produkte

Country Status (7)

Country Link
US (1) US5919673A (de)
EP (1) EP0817862B1 (de)
AT (1) ATE232908T1 (de)
AU (1) AU5257096A (de)
DE (1) DE69626277T2 (de)
DK (1) DK0817862T3 (de)
WO (1) WO1996029424A1 (de)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9620209D0 (en) * 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
US6274320B1 (en) * 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US7244559B2 (en) * 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US7211390B2 (en) * 1999-09-16 2007-05-01 454 Life Sciences Corporation Method of sequencing a nucleic acid
US7575865B2 (en) * 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
AU2004209001B2 (en) * 2003-01-29 2007-10-11 454 Life Sciences Corporation Bead emulsion nucleic acid amplification
KR20210126019A (ko) 2019-01-15 2021-10-19 옵팀비아, 엘엘씨 엔지니어링된 아릴 설페이트-의존 효소
EP3997238A1 (de) 2019-07-09 2022-05-18 Optimvia, LLC Verfahren zur synthese von antikoagulierenden polysacchariden
CN112708569B (zh) * 2019-10-24 2022-11-01 华熙生物科技股份有限公司 发酵生产硫酸软骨素的酵母工程菌及其应用
WO2021199445A1 (en) * 2020-04-03 2021-10-07 Rensselaer Polytechnic Institute Method for producing sulfated polysaccharide and method for producing paps
CN113046402B (zh) * 2020-09-30 2023-04-28 江南大学 一种基于构建双功能酶合成paps的方法
WO2024222534A1 (zh) * 2023-04-28 2024-10-31 江南大学 一种基于aps和paps为活性磺酸供体的再生系统构建与应用方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4266048A (en) * 1977-10-11 1981-05-05 The United States Of America As Represented By The Department Of Health, Education And Welfare Synthesis of analogs of 3'-phosphoadenosine 5'-phosphosulfate (PAPS)
JP3098591B2 (ja) * 1991-11-14 2000-10-16 ユニチカ株式会社 ３’−ホスホアデノシン−５’−ホスホ硫酸の製造方法

Also Published As

Publication number Publication date
DK0817862T3 (da) 2003-06-02
DE69626277D1 (de) 2003-03-27
EP0817862B1 (de) 2003-02-19
EP0817862A4 (de) 1998-10-21
AU5257096A (en) 1996-10-08
DE69626277T2 (de) 2004-01-29
WO1996029424A1 (en) 1996-09-26
US5919673A (en) 1999-07-06
EP0817862A1 (de) 1998-01-14

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