ATE235045T1 - Verfahren zur erfassung von fluoreszierenden molekülen oder anderen teilchen mittels erzeugenden funktionen - Google Patents
Verfahren zur erfassung von fluoreszierenden molekülen oder anderen teilchen mittels erzeugenden funktionenInfo
- Publication number
- ATE235045T1 ATE235045T1 AT00927110T AT00927110T ATE235045T1 AT E235045 T1 ATE235045 T1 AT E235045T1 AT 00927110 T AT00927110 T AT 00927110T AT 00927110 T AT00927110 T AT 00927110T AT E235045 T1 ATE235045 T1 AT E235045T1
- Authority
- AT
- Austria
- Prior art keywords
- particles
- numbers
- photon counts
- fluorescent molecules
- photon
- Prior art date
Links
- 239000002245 particle Substances 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 3
- 238000004364 calculation method Methods 0.000 abstract 1
- 238000005315 distribution function Methods 0.000 abstract 1
- 238000005259 measurement Methods 0.000 abstract 1
- 238000012544 monitoring process Methods 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/28—Investigating the spectrum
- G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
- G01J3/4406—Fluorescence spectrometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/28—Investigating the spectrum
- G01J3/457—Correlation spectrometry, e.g. of the intensity
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/28—Investigating the spectrum
- G01J2003/2866—Markers; Calibrating of scan
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
- G01N2021/6421—Measuring at two or more wavelengths
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6463—Optics
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
Landscapes
- Physics & Mathematics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- General Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Inks, Pencil-Leads, Or Crayons (AREA)
- Spectrometry And Color Measurement (AREA)
Applications Claiming Priority (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US13165799P | 1999-04-29 | 1999-04-29 | |
| EP99112104A EP1063502A1 (de) | 1999-06-23 | 1999-06-23 | Verfahren zur Charakterisierung fluoreszierender Moleküle oder anderer Teilchen unter Verwendung von erzeugenden Funktionen |
| US09/338,439 US6376843B1 (en) | 1999-06-23 | 1999-06-23 | Method of characterizing fluorescent molecules or other particles using generating functions |
| EP99125745 | 1999-12-23 | ||
| US18156400P | 2000-02-10 | 2000-02-10 | |
| PCT/EP2000/003890 WO2000066985A1 (en) | 1999-04-29 | 2000-04-29 | A method of characterizing fluorescent molecules or other particles using generating functions |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE235045T1 true ATE235045T1 (de) | 2003-04-15 |
Family
ID=56290015
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT00927110T ATE235045T1 (de) | 1999-04-29 | 2000-04-29 | Verfahren zur erfassung von fluoreszierenden molekülen oder anderen teilchen mittels erzeugenden funktionen |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP1175602B1 (de) |
| JP (1) | JP3559525B2 (de) |
| AT (1) | ATE235045T1 (de) |
| AU (1) | AU4559600A (de) |
| DE (1) | DE60001731T2 (de) |
| DK (1) | DK1175602T3 (de) |
| WO (1) | WO2000066985A1 (de) |
Families Citing this family (52)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6690463B2 (en) * | 2000-02-10 | 2004-02-10 | Evotec Biosystems Ag | Fluorescence intensity and lifetime distribution analysis |
| CA2440183A1 (en) * | 2001-03-09 | 2002-09-19 | Gnothis Holding Sa | Determination of analytes by means of fluorescence correlation spectroscopy |
| DE10117723A1 (de) * | 2001-04-09 | 2002-10-17 | Evotec Ag | Probenträger, insbesondere für biochemische Reaktionen |
| US6658143B2 (en) * | 2002-04-29 | 2003-12-02 | Amersham Biosciences Corp. | Ray-based image analysis for biological specimens |
| EP1670943B1 (de) | 2003-09-30 | 2013-11-13 | Evotec International GmbH | Diagnostische und therapeutische verwendung einer sulfotransferase für alzheimer-krankheit |
| JP3793531B2 (ja) | 2003-10-07 | 2006-07-05 | オリンパス株式会社 | 蛍光寿命測定装置 |
| JP2008508893A (ja) | 2004-08-11 | 2008-03-27 | エボテツク・ニユーロサイエンシーズ・ゲー・エム・ベー・ハー | 形質膜atpアーゼの診断及び治療への使用 |
| WO2006062127A1 (ja) * | 2004-12-10 | 2006-06-15 | Sysmex Corporation | 免疫システム異常疾患の診断支援方法及び診断支援情報出力装置 |
| ATE530667T1 (de) | 2005-05-27 | 2011-11-15 | Evotec Neurosciences Gmbh | Kcnn3 als diagnostisches und therapeutisches target für alzheimer-krankheit |
| US8114620B2 (en) | 2005-05-30 | 2012-02-14 | Takeda Pharmaceutical Company Limited | Diagnostic and therapeutic target PRKX proteins for neurodegenerative diseases |
| EP1891234B1 (de) | 2005-06-01 | 2014-12-10 | Evotec International GmbH | Verwendung von slc39a12-proteinen als zielmoleküle in der diagnose von und beim screening von medikamenten zur behandlung von alzheimer-krankheit |
| WO2006134128A2 (en) | 2005-06-16 | 2006-12-21 | Evotec Neurosciences Gmbh | Diagnostic and therapeutic target adarb2 proteins for neurodegenerative diseases |
| JP2009278866A (ja) * | 2006-09-04 | 2009-12-03 | Olympus Corp | Dnaに於けるdna結合性タンパク質の結合部位の検出方法 |
| EP2053397A1 (de) | 2007-10-25 | 2009-04-29 | EVOTEC Neurosciences GmbH | PPM1E-Proteine und Nukleinsäuren als Targets für neurodegenerative Erkrankungen |
| KR101324029B1 (ko) * | 2009-01-21 | 2013-11-01 | 레어 라이트, 인크. | 저비용 및 강인한 분광을 위한 상관 간섭 측정 방법, 장치 및 시스템 |
| EP2543990B1 (de) * | 2010-03-01 | 2019-06-26 | Olympus Corporation | Vorrichtung für optische analyse, verfahren für optische analyse und computerprogramm für optische analyse |
| WO2012014778A1 (ja) | 2010-07-26 | 2012-02-02 | オリンパス株式会社 | 発光プローブを用いて溶液中の希薄粒子を検出する方法 |
| WO2012032981A1 (ja) | 2010-09-10 | 2012-03-15 | オリンパス株式会社 | 単一発光粒子の光強度を用いた光分析方法 |
| CN103221806B (zh) | 2010-09-10 | 2015-11-25 | 奥林巴斯株式会社 | 使用两个以上的波长带的光的测量的光学分析方法 |
| JP5737704B2 (ja) * | 2010-09-27 | 2015-06-17 | 株式会社ライフテック | 蛍光一粒子検出方法および検出システム |
| CN103210302B (zh) | 2010-10-19 | 2015-05-27 | 奥林巴斯株式会社 | 观测单个发光粒子的偏振特性的光分析装置、光分析方法 |
| WO2012070414A1 (ja) | 2010-11-25 | 2012-05-31 | オリンパス株式会社 | 単一発光粒子の光の波長特性を用いた光分析装置及び光分析方法 |
| WO2012099234A1 (ja) | 2011-01-20 | 2012-07-26 | オリンパス株式会社 | 単一発光粒子からの光の検出を用いた光分析方法及び光分析装置 |
| EP2669376B1 (de) | 2011-01-26 | 2017-08-16 | Olympus Corporation | Verfahren zur identifizierung von polymorphismen von nukleinsäuremolekülen |
| JP5856984B2 (ja) | 2011-01-26 | 2016-02-10 | オリンパス株式会社 | 核酸分子の多型識別方法 |
| JP5904996B2 (ja) | 2011-03-29 | 2016-04-20 | オリンパス株式会社 | 単一発光粒子検出を用いた光分析装置、光分析方法並びに光分析用コンピュータプログラム |
| EP2706346A4 (de) | 2011-04-13 | 2014-11-19 | Olympus Corp | Photoanalysevorrichtung mit erkennung einzelner lichtemittierender teilchen, photoanalyseverfahren und computerprogramm zur photoanalyse |
| EP2700935A4 (de) | 2011-04-18 | 2014-10-22 | Olympus Corp | Quantitatives bestimmungsverfahren für zielpartikel, photometrische analysevorrichtung und computerprogramm für photometrische analysen |
| EP2743682B1 (de) | 2011-08-11 | 2017-05-31 | Olympus Corporation | Verfahren zum nachweis von zielpartikeln |
| EP2746748B1 (de) | 2011-08-15 | 2017-12-06 | Olympus Corporation | Fotometrische analysevorrichtung mit erkennung einzelner lichtemittierender partikel, verfahren für fotometrische analyse und computerprogramm für fotometrische analyse |
| CN103765195B (zh) | 2011-08-26 | 2018-06-01 | 奥林巴斯株式会社 | 利用光分析的单个粒子检测装置、单个粒子检测方法以及单个粒子检测用计算机程序 |
| CN103765196B (zh) | 2011-08-26 | 2016-03-02 | 奥林巴斯株式会社 | 利用单个发光粒子检测的光分析装置及光分析方法 |
| JP6010034B2 (ja) | 2011-08-30 | 2016-10-19 | オリンパス株式会社 | 標的粒子の検出方法 |
| JP6010035B2 (ja) | 2011-08-30 | 2016-10-19 | オリンパス株式会社 | 単一発光粒子検出を用いた光分析装置、光分析方法及び光分析用コンピュータプログラム |
| WO2013069504A1 (ja) | 2011-11-10 | 2013-05-16 | オリンパス株式会社 | 単一発光粒子検出を用いた光分析装置、光分析方法及び光分析用コンピュータプログラム |
| DE102012100098B4 (de) * | 2012-01-06 | 2021-09-16 | Becker & Hickl Gmbh | Verfahren zur Aufzeichnung von zeitlichen Änderungen der Zeitfunktion eines optischen Signals mit räumlicher Auflösung entlang einer Linie im Raum |
| CN104115001B (zh) | 2012-02-17 | 2016-08-24 | 奥林巴斯株式会社 | 利用单个粒子检测的光分析装置、光分析方法 |
| CN104115003B (zh) | 2012-02-22 | 2016-03-23 | 奥林巴斯株式会社 | 目标粒子的检测方法 |
| JP6095645B2 (ja) | 2012-03-21 | 2017-03-15 | オリンパス株式会社 | 標的核酸分子の検出方法 |
| JP5940651B2 (ja) | 2012-04-18 | 2016-06-29 | オリンパス株式会社 | 標的粒子の検出方法 |
| WO2013157319A1 (ja) | 2012-04-18 | 2013-10-24 | オリンパス株式会社 | 光分析を用いた単一粒子検出装置、単一粒子検出方法及び単一粒子検出用コンピュータプログラム |
| US9245169B2 (en) | 2012-06-15 | 2016-01-26 | Luminex Corporation | Apparatus, system, and method for image normalization using a Gaussian residual of fit selection criteria |
| DE102013213362A1 (de) * | 2013-07-08 | 2015-01-08 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Verfahren zur Identifizierung und Quantifizierung von emittierenden Teilchen in Systemen |
| EP3029505A4 (de) | 2013-07-31 | 2017-03-15 | Olympus Corporation | Optische mikroskopvorrichtung, mikroskopieverfahren und computerprogramm zur mikroskopie mit einer technologie zur erkennung einzelner lichtemittierender partikel |
| JP6313776B2 (ja) | 2013-10-07 | 2018-04-18 | オリンパス株式会社 | 単一発光粒子検出を用いた光分析装置、光分析方法及び光分析用コンピュータプログラム |
| EP2871461A1 (de) * | 2013-11-06 | 2015-05-13 | F. Hoffmann-La Roche AG | Verfahren zur Untersuchung einer Vielzahl von kultivierten Zellen auf Anwesenheit von periodischen Strukturen von mindestens einer Zielkomponente in den kultivierten Zellen |
| WO2017098597A1 (ja) | 2015-12-09 | 2017-06-15 | オリンパス株式会社 | 単一発光粒子検出を用いた光分析方法及び光分析装置 |
| DE102019000066B4 (de) * | 2019-01-09 | 2020-10-08 | Becker & Hickl Gmbh | Verfahren zur Visualisierung von Fluoreszenz-Lifetime-Imaging-Daten |
| EP4204778A4 (de) | 2020-08-27 | 2024-09-25 | The Research Foundation for The State University of New York | System und verfahren für eingebettete diffuse korrelationsspektroskopie |
| CN115048835B (zh) * | 2022-06-09 | 2024-10-18 | 山西大学 | 一种等离子体中粒子瞬态分布的成像方法 |
| KR102593306B1 (ko) * | 2023-03-21 | 2023-10-25 | 부경대학교 산학협력단 | 저투수성 매체 내 형광 추적자 농도분포 추정 장치 및 방법 |
| JP7739535B1 (ja) * | 2024-06-19 | 2025-09-16 | 浜松ホトニクス株式会社 | 信号処理方法、信号処理装置、及び信号処理システム |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1595422A (en) * | 1977-04-28 | 1981-08-12 | Nat Res Dev | Scaning microscopes |
| US5866911A (en) * | 1994-07-15 | 1999-02-02 | Baer; Stephen C. | Method and apparatus for improving resolution in scanned optical system |
| EP0836090A1 (de) * | 1996-10-12 | 1998-04-15 | Evotec BioSystems GmbH | Probenanalyseverfahren durch Bestimmung der spezifischen Helligkeitsverteilung von Teilchen |
| WO1998023941A2 (de) * | 1996-11-27 | 1998-06-04 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V., Berlin | Verfahren und anordnung zum bestimmen vorgegebener eigenschaften von zielpartikeln eines probenmediums |
-
2000
- 2000-04-29 JP JP2000615574A patent/JP3559525B2/ja not_active Expired - Lifetime
- 2000-04-29 DK DK00927110T patent/DK1175602T3/da active
- 2000-04-29 AU AU45596/00A patent/AU4559600A/en not_active Abandoned
- 2000-04-29 DE DE60001731T patent/DE60001731T2/de not_active Expired - Lifetime
- 2000-04-29 WO PCT/EP2000/003890 patent/WO2000066985A1/en not_active Ceased
- 2000-04-29 AT AT00927110T patent/ATE235045T1/de not_active IP Right Cessation
- 2000-04-29 EP EP00927110A patent/EP1175602B1/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| WO2000066985A1 (en) | 2000-11-09 |
| AU4559600A (en) | 2000-11-17 |
| JP2002543414A (ja) | 2002-12-17 |
| DK1175602T3 (da) | 2003-05-26 |
| WO2000066985A9 (en) | 2002-02-14 |
| JP3559525B2 (ja) | 2004-09-02 |
| DE60001731T2 (de) | 2004-02-05 |
| EP1175602B1 (de) | 2003-03-19 |
| EP1175602A1 (de) | 2002-01-30 |
| DE60001731D1 (de) | 2003-04-24 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |