DK1175602T3 - Fremgangsmåde til karakterisering af fluorescente molekyler eller andre partikler, der anvender frembringerfunktioner - Google Patents

Fremgangsmåde til karakterisering af fluorescente molekyler eller andre partikler, der anvender frembringerfunktioner

Info

Publication number
DK1175602T3
DK1175602T3 DK00927110T DK00927110T DK1175602T3 DK 1175602 T3 DK1175602 T3 DK 1175602T3 DK 00927110 T DK00927110 T DK 00927110T DK 00927110 T DK00927110 T DK 00927110T DK 1175602 T3 DK1175602 T3 DK 1175602T3
Authority
DK
Denmark
Prior art keywords
particles
numbers
photon counts
fluorescent molecules
photon
Prior art date
Application number
DK00927110T
Other languages
Danish (da)
English (en)
Inventor
Kauko Palo
Pete Kask
Original Assignee
Evotec Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/338,439 external-priority patent/US6376843B1/en
Priority claimed from EP99112104A external-priority patent/EP1063502A1/de
Application filed by Evotec Ag filed Critical Evotec Ag
Application granted granted Critical
Publication of DK1175602T3 publication Critical patent/DK1175602T3/da

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
    • G01J3/4406—Fluorescence spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/457—Correlation spectrometry, e.g. of the intensity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J2003/2866—Markers; Calibrating of scan
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6463—Optics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence

Landscapes

  • Physics & Mathematics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • General Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Spectrometry And Color Measurement (AREA)
  • Inks, Pencil-Leads, Or Crayons (AREA)
DK00927110T 1999-04-29 2000-04-29 Fremgangsmåde til karakterisering af fluorescente molekyler eller andre partikler, der anvender frembringerfunktioner DK1175602T3 (da)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US13165799P 1999-04-29 1999-04-29
US09/338,439 US6376843B1 (en) 1999-06-23 1999-06-23 Method of characterizing fluorescent molecules or other particles using generating functions
EP99112104A EP1063502A1 (de) 1999-06-23 1999-06-23 Verfahren zur Charakterisierung fluoreszierender Moleküle oder anderer Teilchen unter Verwendung von erzeugenden Funktionen
EP99125745 1999-12-23
US18156400P 2000-02-10 2000-02-10
PCT/EP2000/003890 WO2000066985A1 (en) 1999-04-29 2000-04-29 A method of characterizing fluorescent molecules or other particles using generating functions

Publications (1)

Publication Number Publication Date
DK1175602T3 true DK1175602T3 (da) 2003-05-26

Family

ID=56290015

Family Applications (1)

Application Number Title Priority Date Filing Date
DK00927110T DK1175602T3 (da) 1999-04-29 2000-04-29 Fremgangsmåde til karakterisering af fluorescente molekyler eller andre partikler, der anvender frembringerfunktioner

Country Status (7)

Country Link
EP (1) EP1175602B1 (de)
JP (1) JP3559525B2 (de)
AT (1) ATE235045T1 (de)
AU (1) AU4559600A (de)
DE (1) DE60001731T2 (de)
DK (1) DK1175602T3 (de)
WO (1) WO2000066985A1 (de)

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CN103221806B (zh) 2010-09-10 2015-11-25 奥林巴斯株式会社 使用两个以上的波长带的光的测量的光学分析方法
JP5737704B2 (ja) * 2010-09-27 2015-06-17 株式会社ライフテック 蛍光一粒子検出方法および検出システム
CN103210302B (zh) 2010-10-19 2015-05-27 奥林巴斯株式会社 观测单个发光粒子的偏振特性的光分析装置、光分析方法
WO2012070414A1 (ja) 2010-11-25 2012-05-31 オリンパス株式会社 単一発光粒子の光の波長特性を用いた光分析装置及び光分析方法
JP5856983B2 (ja) 2011-01-20 2016-02-10 オリンパス株式会社 単一発光粒子からの光の検出を用いた光分析方法及び光分析装置
EP2669663B1 (de) 2011-01-26 2017-09-27 Olympus Corporation Verfahren zur identifizierung von polymorphismen von nukleinsäuremolekülen
EP2669376B1 (de) 2011-01-26 2017-08-16 Olympus Corporation Verfahren zur identifizierung von polymorphismen von nukleinsäuremolekülen
WO2012133292A1 (ja) 2011-03-29 2012-10-04 オリンパス株式会社 単一発光粒子検出を用いた光分析装置、光分析方法並びに光分析用コンピュータプログラム
JP5885738B2 (ja) 2011-04-13 2016-03-15 オリンパス株式会社 単一発光粒子検出を用いた光分析装置、光分析方法及び光分析用コンピュータプログラム
JP6013328B2 (ja) 2011-04-18 2016-10-25 オリンパス株式会社 標的粒子の定量方法
JP6013335B2 (ja) 2011-08-11 2016-10-25 オリンパス株式会社 標的粒子の計数方法
CN103733049B (zh) 2011-08-15 2016-01-20 奥林巴斯株式会社 利用单个发光粒子检测的光分析装置、光分析方法以及光分析用计算机程序
WO2013031439A1 (ja) 2011-08-26 2013-03-07 オリンパス株式会社 単一発光粒子検出を用いた光分析装置、光分析方法及び光分析用コンピュータプログラム
WO2013031309A1 (ja) 2011-08-26 2013-03-07 オリンパス株式会社 光分析を用いた単一粒子検出装置、単一粒子検出方法及び単一粒子検出用コンピュータプログラム
JP6010035B2 (ja) 2011-08-30 2016-10-19 オリンパス株式会社 単一発光粒子検出を用いた光分析装置、光分析方法及び光分析用コンピュータプログラム
EP2752655A4 (de) 2011-08-30 2015-06-17 Olympus Corp Verfahren zum nachweis von zielpartikeln
CN103930768B (zh) 2011-11-10 2016-05-18 奥林巴斯株式会社 利用单个发光粒子检测的光分析装置、光分析方法以及光分析用计算机程序
DE102012100098B4 (de) * 2012-01-06 2021-09-16 Becker & Hickl Gmbh Verfahren zur Aufzeichnung von zeitlichen Änderungen der Zeitfunktion eines optischen Signals mit räumlicher Auflösung entlang einer Linie im Raum
WO2013121905A1 (ja) 2012-02-17 2013-08-22 オリンパス株式会社 単一粒子検出を用いた光分析装置、光分析方法及び光分析用コンピュータプログラム
JP5940644B2 (ja) 2012-02-22 2016-06-29 オリンパス株式会社 標的粒子の検出方法
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WO2013157283A1 (ja) 2012-04-18 2013-10-24 オリンパス株式会社 標的粒子の検出方法
CN104541152B (zh) * 2012-06-15 2017-08-25 卢米尼克斯股份有限公司 使用拟合选择标准的高斯残差的图像归一化装置、系统和方法
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WO2015015951A1 (ja) 2013-07-31 2015-02-05 オリンパス株式会社 単一発光粒子検出技術を用いた光学顕微鏡装置、顕微鏡観察法及び顕微鏡観察のためのコンピュータプログラム
CN105593667A (zh) 2013-10-07 2016-05-18 奥林巴斯株式会社 利用单个发光粒子检测的光分析装置、光分析方法以及光分析用计算机程序
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JPWO2017098597A1 (ja) 2015-12-09 2018-10-11 オリンパス株式会社 単一発光粒子検出を用いた光分析方法及び光分析装置
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CN115048835B (zh) * 2022-06-09 2024-10-18 山西大学 一种等离子体中粒子瞬态分布的成像方法
KR102593306B1 (ko) * 2023-03-21 2023-10-25 부경대학교 산학협력단 저투수성 매체 내 형광 추적자 농도분포 추정 장치 및 방법
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Also Published As

Publication number Publication date
JP3559525B2 (ja) 2004-09-02
JP2002543414A (ja) 2002-12-17
EP1175602B1 (de) 2003-03-19
DE60001731D1 (de) 2003-04-24
WO2000066985A9 (en) 2002-02-14
EP1175602A1 (de) 2002-01-30
AU4559600A (en) 2000-11-17
ATE235045T1 (de) 2003-04-15
WO2000066985A1 (en) 2000-11-09
DE60001731T2 (de) 2004-02-05

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