ATE236984T1 - Verfahren zur herstellung von hydrophoben polypeptiden, proteinen und peptiden - Google Patents

Verfahren zur herstellung von hydrophoben polypeptiden, proteinen und peptiden

Info

Publication number: ATE236984T1
Authority: AT; Austria
Prior art keywords: proteins; peptides; hydrophobic polypeptides; producing hydrophobic; purification
Prior art date: 1993-07-06

Application number

AT94109676T

Other languages

English (en)

Inventor

Heinz Doebeli

Nicholas Draeger

Trottman Gerda Huber

Peter Jakob

Dietrich Stueber

Original Assignee

Hoffmann La Roche

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1993-07-06

Filing date

1994-06-23

Publication date

2003-04-15

1994-06-23 Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche

2003-04-15 Application granted granted Critical

2003-04-15 Publication of ATE236984T1 publication Critical patent/ATE236984T1/de

Links

108090000765 processed proteins & peptides Proteins 0.000 title abstract 8
102000004196 processed proteins & peptides Human genes 0.000 title abstract 6
230000002209 hydrophobic effect Effects 0.000 title abstract 3
238000004519 manufacturing process Methods 0.000 title abstract 3
229920001184 polypeptide Polymers 0.000 title abstract 3
102000004169 proteins and genes Human genes 0.000 title abstract 3
108090000623 proteins and genes Proteins 0.000 title abstract 3
238000000746 purification Methods 0.000 abstract 2
239000003814 drug Substances 0.000 abstract 1
229940079593 drug Drugs 0.000 abstract 1
102000037865 fusion proteins Human genes 0.000 abstract 1
108020001507 fusion proteins Proteins 0.000 abstract 1
230000002401 inhibitory effect Effects 0.000 abstract 1
238000000034 method Methods 0.000 abstract 1
238000012216 screening Methods 0.000 abstract 1
230000001988 toxicity Effects 0.000 abstract 1
231100000419 toxicity Toxicity 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4711—Alzheimer's disease; Amyloid plaque core protein
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16111—Human Immunodeficiency Virus, HIV concerning HIV env
- C12N2740/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Genetics & Genomics (AREA)
Zoology (AREA)
Molecular Biology (AREA)
Biomedical Technology (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
Wood Science & Technology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
General Engineering & Computer Science (AREA)
Biotechnology (AREA)
Biophysics (AREA)
Medicinal Chemistry (AREA)
Microbiology (AREA)
Neurology (AREA)
Gastroenterology & Hepatology (AREA)
Chemical Kinetics & Catalysis (AREA)
General Chemical & Material Sciences (AREA)
Virology (AREA)
Toxicology (AREA)
Physics & Mathematics (AREA)
Plant Pathology (AREA)
Neurosurgery (AREA)
Hospice & Palliative Care (AREA)
Public Health (AREA)
Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
Veterinary Medicine (AREA)
Pharmacology & Pharmacy (AREA)
Psychiatry (AREA)
Animal Behavior & Ethology (AREA)
Peptides Or Proteins (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)

AT94109676T 1993-07-06 1994-06-23 Verfahren zur herstellung von hydrophoben polypeptiden, proteinen und peptiden ATE236984T1 (de)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
EP93110755		1993-07-06

Publications (1)

Publication Number	Publication Date
ATE236984T1 true ATE236984T1 (de)	2003-04-15

Family

ID=8213046

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT94109676T ATE236984T1 (de)	1993-07-06	1994-06-23	Verfahren zur herstellung von hydrophoben polypeptiden, proteinen und peptiden

Country Status (14)

Country	Link
US (1)	US5750374A (de)
EP (2)	EP1304381A3 (de)
JP (2)	JP2947401B2 (de)
CN (3)	CN100439391C (de)
AT (1)	ATE236984T1 (de)
AU (1)	AU666274B2 (de)
CA (1)	CA2125467C (de)
DE (1)	DE69432449T2 (de)
DK (1)	DK0641861T3 (de)
ES (1)	ES2194017T3 (de)
NZ (1)	NZ260907A (de)
PT (1)	PT641861E (de)
RU (1)	RU94023251A (de)
ZA (1)	ZA944686B (de)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CA2214247C (en)	1995-03-14	2004-02-10	Praecis Pharmaceuticals Incorporated	Modulators of amyloid aggregation
US5854215A (en) *	1995-03-14	1998-12-29	Praecis Pharmaceuticals Incorporated	Modulators of β-amyloid peptide aggregation
US6303567B1 (en)	1995-03-14	2001-10-16	Praecis Pharmaceuticals, Inc .	Modulators of β-amyloid peptide aggregation comprising D-amino acids
US5817626A (en) *	1995-03-14	1998-10-06	Praecis Pharmaceuticals Incorporated	Modulators of beta-amyloid peptide aggregation
GB9618952D0 (en)	1996-09-11	1996-10-23	Sandoz Ltd	Process
US5985242A (en) *	1995-10-27	1999-11-16	Praecis Pharmaceuticals, Inc.	Modulators of β-amyloid peptide aggregation comprising D-amino acids
US6277826B1 (en)	1996-08-27	2001-08-21	Praecis Pharmaceuticals, Inc.	Modulators of β-amyloid peptide aggregation comprising D-amino acids
NZ514414A (en)	1999-03-04	2004-02-27	Praecis Pharm Inc	Modulators of beta-amyloid peptide aggregation comprising D-amino acids
AU5602100A (en) *	1999-06-09	2000-12-28	Arch Development Corporation	Recombinant prion-like genes and proteins and materials and methods comprising same
CA2387646A1 (en) *	1999-10-21	2001-04-26	Kalobios, Inc.	A general method for optimizing the expression of heterologous proteins
US7799561B2 (en) *	2002-06-12	2010-09-21	Sigma-Aldrich, Co.	Affinity peptides and method for purification of recombinant proteins
FR2859221B1 (fr) *	2003-08-28	2005-10-14	Centre Nat Rech Scient	Vecteur de co-expression de domaines membranaires de proteines d'enveloppe d'un virus et utilisations
US7741053B2 (en) *	2005-05-13	2010-06-22	Sigma-Aldrich Co.	Processes for purification of recombinant proteins
KR100621354B1 (ko) *	2005-06-14	2006-09-08	광주과학기술원	유비퀴틴을 이용한 아밀로이드-베타 펩티드의 제조방법
WO2010063460A1 (en) *	2008-12-02	2010-06-10	Universität Zürich	Recombinant production of hydrophobic peptides and fusion proteins for use in producing same
JPWO2016047794A1 (ja) *	2014-09-26	2017-09-28	株式会社カネカ	疎水性ペプチドの製造法
WO2016089782A1 (en)	2014-12-01	2016-06-09	Pfenex Inc.	Fusion partners for peptide production
CN105001307B (zh) *	2015-07-31	2016-08-24	南京斯拜科生化实业有限公司	一种溶解难溶多肽的偶联肽链及其在液相色谱中分离纯化的应用
CN119930779A (zh) *	2025-02-10	2025-05-06	南京工业大学	采用淀粉样蛋白基序调节嵌合蛛丝蛋白亲疏水性的方法

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
EP0207044A1 (de) *	1985-06-20	1986-12-30	Monsanto Company	Freisetzung von Peptiden aus Polypeptiden
AU6104586A (en) *	1985-08-12	1987-02-19	Malcolm A. Martin	Aids protein using trple gene
ATE98376T1 (de) *	1986-03-24	1993-12-15	Ortho Pharma Corp	Synthetische htlv-iii-peptid-zusammensetzungen und ihre verwendung.
CA1304886C (en) *	1986-07-10	1992-07-07	Heinz Dobeli	Metal chelate resins
US5047513A (en) *	1986-07-10	1991-09-10	Hoffmann-La Roche Inc.	Metal chelate resins
EP0289110A3 (de) *	1987-02-02	1990-01-24	Swiss Serum and Vaccine Institute Berne	Konjugierter Malaria-Impfstoff
CA1340522C (en) *	1987-03-10	1999-05-04	Heinz Dobeli	Fusion proteins containing neighbouring histidines for improved purification
US5416007A (en) *	1987-03-20	1995-05-16	Creative Biomolecules, Inc.	Enhanced proteolytic cleavage of recombinant fusion proteins
US5124255A (en) *	1988-03-11	1992-06-23	Abbott Laboratories	CKS method of protein synthesis
IT1226728B (it) *	1988-08-05	1991-02-05	Eniricerche Spa	Coniugato peptidico immunologicamente attivo utile come vaccino antimalaria e metodo di immunizzazione impiegante lo stesso
US5045190A (en) *	1988-11-08	1991-09-03	Carbonell Ruben G	Chromatography apparatus
JPH06507782A (ja) *	1990-06-15	1994-09-08	サイオス　ノバ　インコーポレイテッド	アルツハイマー病のアミロイド形成開症状を示すヒト以外の組換え哺乳動物
WO1992003474A1 (en) *	1990-08-24	1992-03-05	President And Fellows Of Harvard College	METHOD OF INTERFERING WITH FORMATION OF α-ANTICHYMOTRYPSIN-β-PROTEIN COMPLEX AND SYNTHETIC PEPTIDES FOR USE THEREIN
DK0770679T4 (da) *	1990-11-03	2010-05-10	Siemens Healthcare Diagnostics	HCV-specifikke peptider, sammensætninger dertil og anvendelse deraf
NZ243636A (en) *	1991-07-29	1993-06-25	Iaf Biochem Int	Immunoassay and kit for simultaneously detecting antibodies to hiv-1, hiv-2, htlv-1 and htlv-11
US5434050A (en) *	1991-08-13	1995-07-18	Regents Of The University Of Minnesota	Labelled β-amyloid peptide and methods of screening for Alzheimer's disease

1994
- 1994-06-08 CA CA002125467A patent/CA2125467C/en not_active Expired - Fee Related
- 1994-06-23 ES ES94109676T patent/ES2194017T3/es not_active Expired - Lifetime
- 1994-06-23 DK DK94109676T patent/DK0641861T3/da active
- 1994-06-23 DE DE69432449T patent/DE69432449T2/de not_active Expired - Fee Related
- 1994-06-23 EP EP03000052A patent/EP1304381A3/de not_active Withdrawn
- 1994-06-23 PT PT94109676T patent/PT641861E/pt unknown
- 1994-06-23 EP EP94109676A patent/EP0641861B1/de not_active Expired - Lifetime
- 1994-06-23 AT AT94109676T patent/ATE236984T1/de not_active IP Right Cessation
- 1994-06-29 US US08/268,348 patent/US5750374A/en not_active Expired - Fee Related
- 1994-06-29 ZA ZA944686A patent/ZA944686B/xx unknown
- 1994-07-01 NZ NZ260907A patent/NZ260907A/en not_active IP Right Cessation
- 1994-07-01 AU AU66122/94A patent/AU666274B2/en not_active Ceased
- 1994-07-04 CN CNB99101393XA patent/CN100439391C/zh not_active Expired - Fee Related
- 1994-07-04 RU RU94023251/13A patent/RU94023251A/ru unknown
- 1994-07-04 CN CNA2006101006833A patent/CN1916017A/zh active Pending
- 1994-07-04 CN CN94108110A patent/CN1057535C/zh not_active Expired - Fee Related
- 1994-07-05 JP JP6153161A patent/JP2947401B2/ja not_active Expired - Fee Related
1998
- 1998-02-12 JP JP10030205A patent/JPH1143500A/ja active Pending

Also Published As

Publication number	Publication date
EP1304381A3 (de)	2003-05-02
ES2194017T3 (es)	2003-11-16
JP2947401B2 (ja)	1999-09-13
AU666274B2 (en)	1996-02-01
DK0641861T3 (da)	2003-07-28
JPH1143500A (ja)	1999-02-16
EP0641861B1 (de)	2003-04-09
EP1304381A2 (de)	2003-04-23
PT641861E (pt)	2003-07-31
JPH07102000A (ja)	1995-04-18
CN1227846A (zh)	1999-09-08
US5750374A (en)	1998-05-12
NZ260907A (en)	1996-06-25
CN100439391C (zh)	2008-12-03
CN1916017A (zh)	2007-02-21
RU94023251A (ru)	1996-05-10
CA2125467C (en)	2001-02-06
CN1057535C (zh)	2000-10-18
CA2125467A1 (en)	1995-01-07
EP0641861A2 (de)	1995-03-08
AU6612294A (en)	1995-01-27
EP0641861A3 (de)	1997-07-30
DE69432449D1 (de)	2003-05-15
DE69432449T2 (de)	2004-04-08
CN1098416A (zh)	1995-02-08
ZA944686B (en)	1995-01-06

Legal Events

Date

Code

Title

Description

2003-11-15

UEP

Publication of translation of european patent specification

Ref document number: 0641861

Country of ref document: EP

2010-03-15

REN

Ceased due to non-payment of the annual fee

Publication	Publication Date	Title
DE69432449D1 (de)	2003-05-15	Verfahren zur Herstellung von hydrophoben Polypeptiden, Proteinen und Peptiden
DE69020143D1 (de)	1995-07-20	Verfahren zur verwendung und herstellung von peptiden.
DE69122494D1 (de)	1996-11-07	Vorrichtung zur transdermalen Applikation von Protein- oder Peptid-Medikamenten
DE69019074D1 (de)	1995-06-08	Verfahren zur Fraktionierung von Plasmaproteinen.
NZ224057A (en)	1991-04-26	A polypeptide of a vascular anticoagulant protein and method of preparation
EP0842429A4 (de)	2000-05-03	Verfahren zur charakterisierung der feinstruktur von proteinbindungsstellen
ES550943A0 (es)	1988-05-16	Un procedimiento para preparar una proteina de fusion derivada de la secuencia de aminoacidos de la preprorrelaxina humana.
ATE204291T1 (de)	2001-09-15	Verfahren zur herstellung von peptiden und n- carbamoyl-geschützten peptiden
ES2079621T3 (es)	1996-01-16	Procedimiento de identificacion o de titulacion de proteinas y aplicaciones.
ES2123582T3 (es)	1999-01-16	Proteina con capacidad de formacion de huesos y procedimiento para su produccion.
ES2110996T3 (es)	1998-03-01	Alergeno de polen de ballico.
DE69010155D1 (de)	1994-07-28	Verfahren zur Trennung von Aminosäuren.
EP0537981A3 (de)	1994-10-12	Verfahren zur Spaltung von Acylthiohydantoinen und Anwendung davon zur C-terminalen Peptidsequenzierung.
EP1780219A3 (de)	2008-10-01	Protein-S-Polypeptide und deren Verwendung
ATE189232T1 (de)	2000-02-15	Protein-s polypeptide und deren verwendungen
ATE139572T1 (de)	1996-07-15	Verfahren zur reinigung von rekombinanten proteinen und dafür nützliche produkte
DE59702207D1 (de)	2000-09-21	Verfahren zur eignungsprüfung von faktor viii-haltigen proteinfraktionen
DE59004279D1 (de)	1994-03-03	Verfahren zur C-terminalen Modifizierung von Proteinen.
ATE202114T1 (de)	2001-06-15	Verwendung von peptiden welche eine immunantwort bewirken zur herstellung von medikamenten zur induzierung der immunsuppression.
DE69328488D1 (de)	2000-05-31	Peptide, analoge und ihre mischungen zur detektion und erzeugung von antikörpern gegen die e1 und e2 proteine des rubella virus
DE69531955D1 (de)	2003-11-20	PROGRESSIVE ANDERUNG VON SIGNAL-PROTEINEN UND PEPTIDEn UND DIE HERGESTELLTEN SUPERAGONISTEN UND ANTAGONISTEN
DE69111637D1 (de)	1995-09-07	Verfahren zur Trennung und Konzentration von Phosphopeptiden.
DE69220855D1 (de)	1997-08-21	Vorrichtung und Verfahren zur elektrophoretischen Trennung von Proteinen
DE3485883D1 (de)	1992-09-24	Rekombinante materialien und verfahren zur herstellung von menschliche konnektive gewebe aktivierende peptide iii und deren analoge.
DE3888078D1 (de)	1994-04-07	Veränderungsverfahren für Peptide und Proteine.