ATE139572T1 - Verfahren zur reinigung von rekombinanten proteinen und dafür nützliche produkte - Google Patents
Verfahren zur reinigung von rekombinanten proteinen und dafür nützliche produkteInfo
- Publication number
- ATE139572T1 ATE139572T1 AT91907916T AT91907916T ATE139572T1 AT E139572 T1 ATE139572 T1 AT E139572T1 AT 91907916 T AT91907916 T AT 91907916T AT 91907916 T AT91907916 T AT 91907916T AT E139572 T1 ATE139572 T1 AT E139572T1
- Authority
- AT
- Austria
- Prior art keywords
- disclosed
- fusion proteins
- recombinant proteins
- useful products
- purifying
- Prior art date
Links
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 title abstract 3
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 title abstract 3
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 6
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 5
- 239000002184 metal Substances 0.000 abstract 3
- 229920001184 polypeptide Polymers 0.000 abstract 3
- 102000037865 fusion proteins Human genes 0.000 abstract 2
- 108020001507 fusion proteins Proteins 0.000 abstract 2
- 102000003902 Cathepsin C Human genes 0.000 abstract 1
- 108090000267 Cathepsin C Proteins 0.000 abstract 1
- 125000000487 histidyl group Chemical group [H]N([H])C(C(=O)O*)C([H])([H])C1=C([H])N([H])C([H])=N1 0.000 abstract 1
- 229910021645 metal ion Inorganic materials 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/04—Linear peptides containing only normal peptide links
- C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/04—Linear peptides containing only normal peptide links
- C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16311—Human Immunodeficiency Virus, HIV concerning HIV regulatory proteins
- C12N2740/16322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Virology (AREA)
- Gastroenterology & Hepatology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US50660590A | 1990-04-09 | 1990-04-09 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE139572T1 true ATE139572T1 (de) | 1996-07-15 |
Family
ID=24015282
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT91907916T ATE139572T1 (de) | 1990-04-09 | 1991-03-11 | Verfahren zur reinigung von rekombinanten proteinen und dafür nützliche produkte |
Country Status (9)
| Country | Link |
|---|---|
| EP (1) | EP0527778B1 (de) |
| JP (1) | JP3346563B2 (de) |
| AT (1) | ATE139572T1 (de) |
| AU (1) | AU7685791A (de) |
| DE (1) | DE69120409T2 (de) |
| DK (1) | DK0527778T3 (de) |
| ES (1) | ES2088495T3 (de) |
| GR (1) | GR3021095T3 (de) |
| WO (1) | WO1991015589A1 (de) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| HUT69963A (en) * | 1990-12-13 | 1995-09-28 | Upjohn Co | Non-naturally-occuring fusion proteins and process for preparing them |
| WO1995030685A1 (en) * | 1994-05-09 | 1995-11-16 | Unizyme Laboratories A/S | An enzymatic process for producing a desired protein from an amino terminal extended protein |
| ES2108652B1 (es) * | 1996-04-19 | 1998-07-16 | Antibioticos Sa | Un procedimiento para modificar la enzima 7/3-(4-carboxibutanamido) cefalosporinacilasa y purificarla en un solo paso cromatografico. |
| CN1953992A (zh) | 2004-06-23 | 2007-04-25 | Usv有限公司 | 源自胎盘和脑垂体同种型的嵌合体人生长激素和获得所述嵌合体的方法 |
| FR2892725B1 (fr) * | 2005-10-31 | 2011-03-04 | Flamel Tech Sa | Acides polyglutamiques fonctionnalises par des derives de l'histidine et des groupements hydrophobes et leurs applications notamment therapeutiques |
| ES2524786T5 (es) * | 2007-08-06 | 2019-06-19 | Max Planck Gesellschaft | Uso de quelantes inmovilizados para purificación de proteínas recombinantes por cromatografía de iones metálicos inmovilizados y método de purificación de proteínas recombinantes |
| CN105273059B (zh) * | 2015-11-27 | 2018-07-20 | 福州大学 | 一种章鱼钙螯合蛋白肽及其制备方法 |
| JP7597346B2 (ja) * | 2020-02-19 | 2024-12-10 | 国立大学法人山口大学 | タグペプチド及びポリペプチドの精製方法 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4569794A (en) * | 1984-12-05 | 1986-02-11 | Eli Lilly And Company | Process for purifying proteins and compounds useful in such process |
| CA1340522C (en) * | 1987-03-10 | 1999-05-04 | Heinz Dobeli | Fusion proteins containing neighbouring histidines for improved purification |
| EP0371041A1 (de) * | 1987-06-24 | 1990-06-06 | Novo Nordisk A/S | Verfahren für die herstellung eines proteins oder polypeptides, dns-sequenz, die dieses polypeptid kodiert, mikroorganismus, der diese dns-sequenz beinhaltet, sowie das polypeptid und seine verwendung als pharmazeutisches präparat |
-
1991
- 1991-03-11 DE DE69120409T patent/DE69120409T2/de not_active Expired - Fee Related
- 1991-03-11 EP EP91907916A patent/EP0527778B1/de not_active Expired - Lifetime
- 1991-03-11 AU AU76857/91A patent/AU7685791A/en not_active Abandoned
- 1991-03-11 WO PCT/US1991/001543 patent/WO1991015589A1/en not_active Ceased
- 1991-03-11 AT AT91907916T patent/ATE139572T1/de not_active IP Right Cessation
- 1991-03-11 ES ES91907916T patent/ES2088495T3/es not_active Expired - Lifetime
- 1991-03-11 DK DK91907916.0T patent/DK0527778T3/da active
- 1991-03-11 JP JP50774191A patent/JP3346563B2/ja not_active Expired - Fee Related
-
1996
- 1996-09-19 GR GR960402455T patent/GR3021095T3/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| GR3021095T3 (en) | 1996-12-31 |
| WO1991015589A1 (en) | 1991-10-17 |
| DE69120409D1 (de) | 1996-07-25 |
| JP3346563B2 (ja) | 2002-11-18 |
| DK0527778T3 (da) | 1996-10-21 |
| DE69120409T2 (de) | 1996-11-28 |
| AU7685791A (en) | 1991-10-30 |
| EP0527778A1 (de) | 1993-02-24 |
| EP0527778B1 (de) | 1996-06-19 |
| JPH05505940A (ja) | 1993-09-02 |
| ES2088495T3 (es) | 1996-08-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification | ||
| REN | Ceased due to non-payment of the annual fee |