ATE139572T1 - Verfahren zur reinigung von rekombinanten proteinen und dafür nützliche produkte - Google Patents

Verfahren zur reinigung von rekombinanten proteinen und dafür nützliche produkte

Info

Publication number: ATE139572T1
Authority: AT; Austria
Prior art keywords: disclosed; fusion proteins; recombinant proteins; useful products; purifying
Prior art date: 1990-04-09

Application number

AT91907916T

Other languages

English (en)

Inventor

Satish K Sharma

Original Assignee

Upjohn Co

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1990-04-09

Filing date

1991-03-11

Publication date

1996-07-15

1991-03-11 Application filed by Upjohn Co filed Critical Upjohn Co

1996-07-15 Application granted granted Critical

1996-07-15 Publication of ATE139572T1 publication Critical patent/ATE139572T1/de

Links

102000007056 Recombinant Fusion Proteins Human genes 0.000 title abstract 3
108010008281 Recombinant Fusion Proteins Proteins 0.000 title abstract 3
108090000765 processed proteins & peptides Proteins 0.000 abstract 6
102000004196 processed proteins & peptides Human genes 0.000 abstract 5
239000002184 metal Substances 0.000 abstract 3
229920001184 polypeptide Polymers 0.000 abstract 3
102000037865 fusion proteins Human genes 0.000 abstract 2
108020001507 fusion proteins Proteins 0.000 abstract 2
102000003902 Cathepsin C Human genes 0.000 abstract 1
108090000267 Cathepsin C Proteins 0.000 abstract 1
125000000487 histidyl group Chemical group [H]N([H])C(C(=O)O*)C([H])([H])C1=C([H])N([H])C([H])=N1 0.000 abstract 1
229910021645 metal ion Inorganic materials 0.000 abstract 1
239000000758 substrate Substances 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/04—Linear peptides containing only normal peptide links
- C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/04—Linear peptides containing only normal peptide links
- C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16311—Human Immunodeficiency Virus, HIV concerning HIV regulatory proteins
- C12N2740/16322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Genetics & Genomics (AREA)
Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Wood Science & Technology (AREA)
Biophysics (AREA)
Zoology (AREA)
Biomedical Technology (AREA)
Medicinal Chemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
Biotechnology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
General Engineering & Computer Science (AREA)
Microbiology (AREA)
Gastroenterology & Hepatology (AREA)
Virology (AREA)
Physics & Mathematics (AREA)
Plant Pathology (AREA)
Peptides Or Proteins (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Enzymes And Modification Thereof (AREA)

AT91907916T 1990-04-09 1991-03-11 Verfahren zur reinigung von rekombinanten proteinen und dafür nützliche produkte ATE139572T1 (de)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US50660590A	1990-04-09	1990-04-09

Publications (1)

Publication Number	Publication Date
ATE139572T1 true ATE139572T1 (de)	1996-07-15

Family

ID=24015282

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT91907916T ATE139572T1 (de)	1990-04-09	1991-03-11	Verfahren zur reinigung von rekombinanten proteinen und dafür nützliche produkte

Country Status (9)

Country	Link
EP (1)	EP0527778B1 (de)
JP (1)	JP3346563B2 (de)
AT (1)	ATE139572T1 (de)
AU (1)	AU7685791A (de)
DE (1)	DE69120409T2 (de)
DK (1)	DK0527778T3 (de)
ES (1)	ES2088495T3 (de)
GR (1)	GR3021095T3 (de)
WO (1)	WO1991015589A1 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
FI932680L (fi) *	1990-12-13	1993-06-11	Upjohn Co	Fusionspolypeptider
WO1995030685A1 (en) *	1994-05-09	1995-11-16	Unizyme Laboratories A/S	An enzymatic process for producing a desired protein from an amino terminal extended protein
ES2108652B1 (es) *	1996-04-19	1998-07-16	Antibioticos Sa	Un procedimiento para modificar la enzima 7/3-(4-carboxibutanamido) cefalosporinacilasa y purificarla en un solo paso cromatografico.
CA2558760A1 (en)	2004-06-23	2006-01-05	Usv Limited	Chimeric human growth hormone derived from the placenta and pituitary isoform and processes for obtaining said chimera
FR2892725B1 (fr) *	2005-10-31	2011-03-04	Flamel Tech Sa	Acides polyglutamiques fonctionnalises par des derives de l'histidine et des groupements hydrophobes et leurs applications notamment therapeutiques
ES2832541T3 (es) *	2007-08-06	2021-06-10	Max Planck Gesellschaft	Uso de un quelante inmovilizado para la purificación de polipéptidos recombinantes mediante cromatografía de iones metálicos inmovilizados
CN105273059B (zh) *	2015-11-27	2018-07-20	福州大学	一种章鱼钙螯合蛋白肽及其制备方法
JP7597346B2 (ja) *	2020-02-19	2024-12-10	国立大学法人山口大学	タグペプチド及びポリペプチドの精製方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4569794A (en) *	1984-12-05	1986-02-11	Eli Lilly And Company	Process for purifying proteins and compounds useful in such process
CA1340522C (en) *	1987-03-10	1999-05-04	Heinz Dobeli	Fusion proteins containing neighbouring histidines for improved purification
WO1988010299A1 (en) *	1987-06-24	1988-12-29	Novo-Nordisk A/S	A process for preparing a protein or polypeptide, a dna sequence coding for the polypeptide, a microorganism containing the dna sequence as well as the polypeptide and its use as a pharmaceutical preparation

1991
- 1991-03-11 JP JP50774191A patent/JP3346563B2/ja not_active Expired - Fee Related
- 1991-03-11 EP EP91907916A patent/EP0527778B1/de not_active Expired - Lifetime
- 1991-03-11 DE DE69120409T patent/DE69120409T2/de not_active Expired - Fee Related
- 1991-03-11 DK DK91907916.0T patent/DK0527778T3/da active
- 1991-03-11 ES ES91907916T patent/ES2088495T3/es not_active Expired - Lifetime
- 1991-03-11 AT AT91907916T patent/ATE139572T1/de not_active IP Right Cessation
- 1991-03-11 AU AU76857/91A patent/AU7685791A/en not_active Abandoned
- 1991-03-11 WO PCT/US1991/001543 patent/WO1991015589A1/en not_active Ceased
1996
- 1996-09-19 GR GR960402455T patent/GR3021095T3/el unknown

Also Published As

Publication number	Publication date
GR3021095T3 (en)	1996-12-31
AU7685791A (en)	1991-10-30
ES2088495T3 (es)	1996-08-16
JPH05505940A (ja)	1993-09-02
WO1991015589A1 (en)	1991-10-17
DK0527778T3 (da)	1996-10-21
DE69120409D1 (de)	1996-07-25
EP0527778B1 (de)	1996-06-19
DE69120409T2 (de)	1996-11-28
EP0527778A1 (de)	1993-02-24
JP3346563B2 (ja)	2002-11-18

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Legal Events

Date	Code	Title	Description
1997-01-15	UEP	Publication of translation of european patent specification
2008-12-15	REN	Ceased due to non-payment of the annual fee