ATE238344T1 - Synthese und gewinnung von aspartam, unter anwendung eines enzymatischen deformylationsschrittes - Google Patents

Synthese und gewinnung von aspartam, unter anwendung eines enzymatischen deformylationsschrittes

Info

Publication number: ATE238344T1
Authority: AT; Austria
Prior art keywords: enzymatic; aspartame; synthesis; formyl; deformylation
Prior art date: 1998-12-22

Application number

AT99962579T

Other languages

English (en)

Inventor

Peter Jan Leonard M Quaedflieg

Theodorus Sonke

Adolf Fritz Volker Wagner

Original Assignee

Holland Sweetener Co

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1998-12-22

Filing date

1999-12-20

Publication date

2003-05-15

1999-12-20 Application filed by Holland Sweetener Co filed Critical Holland Sweetener Co

1999-12-20 Priority claimed from PCT/NL1999/000787 external-priority patent/WO2000037486A1/en

2003-05-15 Application granted granted Critical

2003-05-15 Publication of ATE238344T1 publication Critical patent/ATE238344T1/de

Links

230000002255 enzymatic effect Effects 0.000 title abstract 6
108010011485 Aspartame Proteins 0.000 title abstract 4
IAOZJIPTCAWIRG-QWRGUYRKSA-N aspartame Chemical compound OC(=O)C[C@H](N)C(=O)N[C@H](C(=O)OC)CC1=CC=CC=C1 IAOZJIPTCAWIRG-QWRGUYRKSA-N 0.000 title abstract 4
229960003438 aspartame Drugs 0.000 title abstract 4
235000010357 aspartame Nutrition 0.000 title abstract 4
239000000605 aspartame Substances 0.000 title abstract 4
230000015572 biosynthetic process Effects 0.000 title abstract 4
238000006344 deformylation reaction Methods 0.000 title abstract 4
238000003786 synthesis reaction Methods 0.000 title abstract 4
230000006198 deformylation Effects 0.000 title abstract 2
108090000790 Enzymes Proteins 0.000 abstract 2
102000004190 Enzymes Human genes 0.000 abstract 2
238000005859 coupling reaction Methods 0.000 abstract 2
238000005580 one pot reaction Methods 0.000 abstract 2
108010008211 N-Formylmethionine Leucyl-Phenylalanine Proteins 0.000 abstract 1
-1 N-formyl- alpha -L-aspartyl-L-phenylalanine compound Chemical class 0.000 abstract 1
MQUUQXIFCBBFDP-VKHMYHEASA-N N-formyl-L-aspartic acid Chemical compound OC(=O)C[C@@H](C(O)=O)NC=O MQUUQXIFCBBFDP-VKHMYHEASA-N 0.000 abstract 1
108010038807 Oligopeptides Proteins 0.000 abstract 1
102000015636 Oligopeptides Human genes 0.000 abstract 1
230000008878 coupling Effects 0.000 abstract 1
230000000694 effects Effects 0.000 abstract 1
229910021645 metal ion Inorganic materials 0.000 abstract 1
VSDUZFOSJDMAFZ-UHFFFAOYSA-N methyl 2-amino-3-phenylpropanoate Chemical compound COC(=O)C(N)CC1=CC=CC=C1 VSDUZFOSJDMAFZ-UHFFFAOYSA-N 0.000 abstract 1
239000000203 mixture Substances 0.000 abstract 1
238000002360 preparation method Methods 0.000 abstract 1
238000011084 recovery Methods 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
- C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
- C07K5/06—Dipeptides
- C07K5/06104—Dipeptides with the first amino acid being acidic
- C07K5/06113—Asp- or Asn-amino acid
- C07K5/06121—Asp- or Asn-amino acid the second amino acid being aromatic or cycloaliphatic
- C07K5/0613—Aspartame
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
- C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)

Landscapes

Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Zoology (AREA)
Engineering & Computer Science (AREA)
Wood Science & Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
Molecular Biology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Biotechnology (AREA)
Microbiology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
General Engineering & Computer Science (AREA)
Medicinal Chemistry (AREA)
Biophysics (AREA)
Biomedical Technology (AREA)
Chemical Kinetics & Catalysis (AREA)
General Chemical & Material Sciences (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Enzymes And Modification Thereof (AREA)
Peptides Or Proteins (AREA)
Seasonings (AREA)

AT99962579T 1998-12-22 1999-12-20 Synthese und gewinnung von aspartam, unter anwendung eines enzymatischen deformylationsschrittes ATE238344T1 (de)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
EP98204373A EP1013663A1 (de)	1998-12-22	1998-12-22	Synthese und Gewinnung von Aspartam unter Anwendung eines enzymatischen Deformylierungsschrittes
PCT/NL1999/000787 WO2000037486A1 (en)	1998-12-22	1999-12-20	Synthesis and recovery of aspartame involving enzymatic deformylation step

Publications (1)

Publication Number	Publication Date
ATE238344T1 true ATE238344T1 (de)	2003-05-15

Family

ID=8234517

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT99962579T ATE238344T1 (de)	1998-12-22	1999-12-20	Synthese und gewinnung von aspartam, unter anwendung eines enzymatischen deformylationsschrittes

Country Status (6)

Country	Link
EP (2)	EP1013663A1 (de)
KR (1)	KR20010099875A (de)
CN (1)	CN1178953C (de)
AT (1)	ATE238344T1 (de)
AU (1)	AU1898500A (de)
DE (1)	DE69907266T2 (de)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CN102864196B (zh) *	2012-10-09	2014-07-02	南京工业大学	一种二氢乳清酸酶法制备α-天冬氨酰小肽的方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS585189A (ja) *	1981-07-01	1983-01-12	Sanraku Inc	新規なアミドヒドロラ−ゼ
JPS60164495A (ja) *	1984-01-16	1985-08-27	モンサント　コンパニー	Ｎ‐ホルミルアミノ酸とペプチド残基の酵素的結合法
IT1176332B (it) *	1984-06-27	1987-08-18	Erba Farmitalia	Procedimento per al preparazione di peptidi
US4745067A (en) *	1985-05-02	1988-05-17	Microbial Chemistry Research Foundation	L-aminoacylases
US5834243A (en) *	1996-07-19	1998-11-10	Bogosian; Gregg	Deformylation of f-Met peptides in bacterial expression systems

1998
- 1998-12-22 EP EP98204373A patent/EP1013663A1/de not_active Withdrawn
1999
- 1999-12-20 DE DE69907266T patent/DE69907266T2/de not_active Expired - Lifetime
- 1999-12-20 EP EP99962579A patent/EP1140982B1/de not_active Expired - Lifetime
- 1999-12-20 AT AT99962579T patent/ATE238344T1/de not_active IP Right Cessation
- 1999-12-20 AU AU18985/00A patent/AU1898500A/en not_active Abandoned
- 1999-12-20 KR KR1020017007869A patent/KR20010099875A/ko not_active Withdrawn
- 1999-12-20 CN CNB998163163A patent/CN1178953C/zh not_active Expired - Fee Related

Also Published As

Publication number	Publication date
CN1335851A (zh)	2002-02-13
KR20010099875A (ko)	2001-11-09
DE69907266T2 (de)	2004-03-25
EP1140982B1 (de)	2003-04-23
CN1178953C (zh)	2004-12-08
DE69907266D1 (de)	2003-05-28
AU1898500A (en)	2000-07-12
EP1140982A1 (de)	2001-10-10
EP1013663A1 (de)	2000-06-28

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Legal Events

Date	Code	Title	Description
2003-10-15	RER	Ceased as to paragraph 5 lit. 3 law introducing patent treaties