ATE238414T1 - Verfahren zur angiostatinrückfaltung - Google Patents

Verfahren zur angiostatinrückfaltung

Info

Publication number
ATE238414T1
ATE238414T1 AT99901279T AT99901279T ATE238414T1 AT E238414 T1 ATE238414 T1 AT E238414T1 AT 99901279 T AT99901279 T AT 99901279T AT 99901279 T AT99901279 T AT 99901279T AT E238414 T1 ATE238414 T1 AT E238414T1
Authority
AT
Austria
Prior art keywords
angiostatin
refolding
disclosed
subfragments
solubilizing
Prior art date
Application number
AT99901279T
Other languages
English (en)
Inventor
Bernard N Violand
Joseph O Polazzi
Gerald F Casperson
Original Assignee
Searle & Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Searle & Co filed Critical Searle & Co
Application granted granted Critical
Publication of ATE238414T1 publication Critical patent/ATE238414T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424Serine endopeptidases (3.4.21)
    • C12N9/6435Plasmin (3.4.21.7), i.e. fibrinolysin
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y304/00Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21Serine endopeptidases (3.4.21)
    • C12Y304/21007Plasmin (3.4.21.7), i.e. fibrinolysin

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
AT99901279T 1998-01-12 1999-01-11 Verfahren zur angiostatinrückfaltung ATE238414T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7124798P 1998-01-12 1998-01-12
PCT/US1999/000048 WO1999035248A2 (en) 1998-01-12 1999-01-11 Method of refolding angiostatin

Publications (1)

Publication Number Publication Date
ATE238414T1 true ATE238414T1 (de) 2003-05-15

Family

ID=22100170

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99901279T ATE238414T1 (de) 1998-01-12 1999-01-11 Verfahren zur angiostatinrückfaltung

Country Status (8)

Country Link
EP (1) EP1047771B1 (de)
AT (1) ATE238414T1 (de)
AU (1) AU2101599A (de)
DE (1) DE69907139T2 (de)
DK (1) DK1047771T3 (de)
ES (1) ES2198879T3 (de)
PT (1) PT1047771E (de)
WO (1) WO1999035248A2 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL140729A0 (en) * 1998-07-14 2002-02-10 Bristol Myers Squibb Co Lysine binding fragments of angiostatin, pharmaceutical compositions containing the same and processes for the preparation thereof
EP1218495A2 (de) * 1999-12-03 2002-07-03 EntreMed, Inc. Methode zur herstellung und reinigung von angiostatin-m(3)
US6723536B2 (en) 1999-12-03 2004-04-20 Entremed, Inc. Method of producing and purifying angiostatin
IL152804A0 (en) 2000-05-16 2003-06-24 Bolder Biotechnology Inc Methods for refolding proteins containing free cysteine residues
AU8591901A (en) 2000-09-05 2002-03-22 Karolinska Innovations Ab Materials and methods relating to endothelial cell growth inhibitors
US20100144622A1 (en) * 2006-09-29 2010-06-10 Xinli Lin Methods for production of recombinant plasminogen and plasmin polypeptides
WO2008076933A2 (en) 2006-12-14 2008-06-26 Bolder Biotechnology, Inc. Long acting proteins and peptides and methods of making and using the same

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2047063A1 (en) * 1991-07-15 1993-01-16 Warren C. Mackellar Method for folding tissue plasminogen activators and erivatives thereof
US6057122A (en) * 1996-05-03 2000-05-02 Abbott Laboratories Antiangiogenic peptides polynucleotides encoding same and methods for inhibiting angiogenesis

Also Published As

Publication number Publication date
WO1999035248A3 (en) 1999-11-11
PT1047771E (pt) 2003-08-29
DE69907139D1 (de) 2003-05-28
AU2101599A (en) 1999-07-26
EP1047771A2 (de) 2000-11-02
WO1999035248A2 (en) 1999-07-15
ES2198879T3 (es) 2004-02-01
DK1047771T3 (da) 2003-08-11
DE69907139T2 (de) 2004-01-29
EP1047771B1 (de) 2003-04-23

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