ATE242260T1 - Sulfonatfreie cyaninfarbstoffe zur markierung von nukleosiden und nukleotiden - Google Patents

Sulfonatfreie cyaninfarbstoffe zur markierung von nukleosiden und nukleotiden

Info

Publication number
ATE242260T1
ATE242260T1 AT98930319T AT98930319T ATE242260T1 AT E242260 T1 ATE242260 T1 AT E242260T1 AT 98930319 T AT98930319 T AT 98930319T AT 98930319 T AT98930319 T AT 98930319T AT E242260 T1 ATE242260 T1 AT E242260T1
Authority
AT
Austria
Prior art keywords
group
integer
nucleosides
nucleotides
sulfonate
Prior art date
Application number
AT98930319T
Other languages
English (en)
Inventor
Charles K Brush
Ned D Reimer
Original Assignee
Amersham Biosciences Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amersham Biosciences Corp filed Critical Amersham Biosciences Corp
Application granted granted Critical
Publication of ATE242260T1 publication Critical patent/ATE242260T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT98930319T 1997-06-20 1998-06-16 Sulfonatfreie cyaninfarbstoffe zur markierung von nukleosiden und nukleotiden ATE242260T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/879,596 US5986086A (en) 1997-06-20 1997-06-20 Non-sulfonated cyanine dyes for labeling nucleosides and nucleotides
PCT/US1998/012593 WO1998058942A1 (en) 1997-06-20 1998-06-16 Non-sulfonated cyanine dyes for labeling nucleosides and nucleotides

Publications (1)

Publication Number Publication Date
ATE242260T1 true ATE242260T1 (de) 2003-06-15

Family

ID=25374472

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98930319T ATE242260T1 (de) 1997-06-20 1998-06-16 Sulfonatfreie cyaninfarbstoffe zur markierung von nukleosiden und nukleotiden

Country Status (7)

Country Link
US (1) US5986086A (de)
EP (1) EP0989990B1 (de)
JP (1) JP2002507203A (de)
AT (1) ATE242260T1 (de)
CA (1) CA2294979A1 (de)
DE (1) DE69815342T2 (de)
WO (1) WO1998058942A1 (de)

Families Citing this family (59)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2319777C (en) 1998-02-04 2008-01-08 Amersham Pharmacia Biotech, Inc. Dideoxy dye terminators
US6114350A (en) * 1999-04-19 2000-09-05 Nen Life Science Products, Inc. Cyanine dyes and synthesis methods thereof
US6271360B1 (en) * 1999-08-27 2001-08-07 Valigen (Us), Inc. Single-stranded oligodeoxynucleotide mutational vectors
CA2382063A1 (en) * 1999-09-17 2001-03-22 Shiv Kumar Charge-modified nucleic acid terminators
EP1088559A3 (de) * 1999-09-29 2002-10-02 INSTITUT FÜR DIAGNOSTIKFORSCHUNG GmbH AN DER FREIEN UNIVERSITÄT BERLIN Galenische Formulierungen
DE60006634T2 (de) 1999-12-07 2004-04-15 Fuji Photo Film Co., Ltd., Minami-Ashigara Fluoreszente materialen
IL150062A0 (en) * 1999-12-17 2002-12-01 Ariad Pharma Inc Proton pump inhibitors
AU2277201A (en) * 1999-12-17 2001-06-25 Ariad Pharmaceuticals, Inc. Novel purines
JP2001288197A (ja) * 2000-04-10 2001-10-16 Fuji Photo Film Co Ltd 蛍光ヌクレオチド
DE10046215B4 (de) * 2000-09-19 2004-04-15 Institut für Chemo- und Biosensorik Münster e.V. i.Ins. Fluorochrome und deren Verwendung
DE60126297T2 (de) † 2000-09-29 2007-11-15 Molecular Probes, Inc., Eugene Modifizierte carbocyaninfarbstoffe und deren konjugate
EP1317464B1 (de) * 2000-10-11 2009-05-13 Applera Corporation Fluoreszierende nukleobasekonjugate mit anionische linker
NO20006130D0 (no) * 2000-12-01 2000-12-01 Erling Sundrehagen Reagens og analysemetode
WO2002068537A2 (en) * 2001-02-28 2002-09-06 Stratagene Fluorescent dye
JP4676654B2 (ja) * 2001-07-19 2011-04-27 富士フイルム株式会社 蛍光ヌクレオチド及びそれを用いた標識法
US6972339B2 (en) 2001-09-07 2005-12-06 Epoch Biosciences, Inc. Compounds and methods for fluorescent labeling
US8569516B2 (en) * 2001-09-07 2013-10-29 Elitech Holding B.V. Compounds and methods for fluorescent labeling
IL146142A0 (en) * 2001-10-24 2002-07-25 Univ Bar Ilan 2-substituted-5'-o-(1-boranotriphosphate adenosine derivatives and pharmaceutical compositions comprising them for treatment of type 2 diabetes
US6838289B2 (en) 2001-11-14 2005-01-04 Beckman Coulter, Inc. Analyte detection system
US7091348B2 (en) * 2002-07-01 2006-08-15 Guava Technologies, Inc. Fluorescent dyes, energy transfer couples and methods
US20040265870A1 (en) * 2003-04-09 2004-12-30 Invitrogen Corporation Methods of synthesizing and labeling nucleic acid molecules
US20050065171A1 (en) * 2003-06-25 2005-03-24 Shakespeare William C. Substituted purine derivatives
US7417726B2 (en) * 2003-09-19 2008-08-26 Applied Biosystems Inc. Normalization of data using controls
US7570443B2 (en) 2003-09-19 2009-08-04 Applied Biosystems, Llc Optical camera alignment
US20060029948A1 (en) * 2003-09-19 2006-02-09 Gary Lim Sealing cover and dye compatibility selection
US7541454B2 (en) * 2003-10-24 2009-06-02 Epoch Biosciences, Inc. Compounds and methods for fluorescent labeling
US7705150B2 (en) * 2004-02-04 2010-04-27 Biosearch Technologies, Inc. Cyanine dyes
WO2005077125A2 (en) * 2004-02-11 2005-08-25 Applera Corporation Methods and compositions for detecting nucleic acids
US20050255485A1 (en) * 2004-05-14 2005-11-17 Livak Kenneth J Detection of gene duplications
EP1781675B1 (de) 2004-08-13 2014-03-26 Epoch Biosciences, Inc. Fluoreszierende phosphonatfarbstoffe und konjugate
EP2312317A3 (de) 2005-05-03 2011-06-08 Life Technologies Corporation Fluoreszentes Erkennungssystem und Farbstoffsatz zur Verwendung damit
US7781187B2 (en) * 2005-12-30 2010-08-24 Corning Incorporated Fluorescent dyes
CA2622649C (en) * 2007-03-09 2018-04-24 Riken Nucleic acid amplification method using primer exhibiting exciton effect
FR2913424B1 (fr) * 2007-03-09 2009-07-24 Cis Bio Internat Sa Derives de cyanine, conjuges fluorescents les contenant et leur utilisation
US8383792B2 (en) 2007-03-09 2013-02-26 Riken Compound having structure derived from mononucleoside or mononucleotide, nucleic acid, labeling substance, and method and kit for detection of nucleic acid
WO2009012109A2 (en) 2007-07-13 2009-01-22 Emory University Cyanine-containing compounds for cancer imaging and treatment
CN102099059B (zh) * 2008-06-13 2015-09-23 西塞医疗中心 用于癌症靶向治疗的小分子配体-药物轭合物
JP4621926B2 (ja) * 2008-07-24 2011-02-02 国立大学法人九州大学 酵素基質修飾ヌクレオシド三リン酸、核酸プローブ、マルチラベル化核酸プローブ、マルチラベル化核酸プローブの製造方法および標的核酸の検出方法
JP5146785B2 (ja) * 2008-07-24 2013-02-20 国立大学法人九州大学 酵素基質修飾ヌクレオシド三リン酸誘導体
JP5704481B2 (ja) * 2010-01-22 2015-04-22 国立大学法人九州大学 核酸検出用キット
US8889886B2 (en) 2010-08-25 2014-11-18 Pacific Biosciences, Inc. Cyanine dyes
WO2012088007A1 (en) 2010-12-21 2012-06-28 Pierce Biotechnology, Inc. Fluorescent compounds
US20120171684A1 (en) 2010-12-29 2012-07-05 Life Technologies Corporation Ddao compounds as fluorescent reference standards
ES2570591T3 (es) 2011-05-24 2016-05-19 Elitechgroup B V Detección de Staphylococcus aureus resistente a meticilina
US8889884B1 (en) 2011-07-14 2014-11-18 Pierce Biotechnology, Inc. Phosphine derivatives of fluorescent compounds
US9249307B2 (en) 2011-08-16 2016-02-02 Pierce Biotechnology, Inc. Benzocyanine compounds
US9751868B2 (en) 2012-02-28 2017-09-05 Pierce Biotechnology, Inc. Benzocyanine compounds
EP2804860B1 (de) 2012-03-02 2016-06-15 Pierce Biotechnology, Inc. Indol-derivate als markierungsfarbstoff für biomoleküle
EP2850086B1 (de) 2012-05-18 2023-07-05 Pacific Biosciences Of California, Inc. Heteroarylcyaninfarbstoffe
US9315864B2 (en) 2012-05-18 2016-04-19 Pacific Biosciences Of California, Inc. Heteroarylcyanine dyes with sulfonic acid substituents
JP5509440B2 (ja) * 2012-06-22 2014-06-04 国立大学法人九州大学 タンパク質−核酸複合体の製造方法および標的物の検出方法
CN104540819B (zh) 2012-08-28 2019-04-19 皮尔斯生物科技有限公司 苯并吡喃鎓化合物
US10294261B2 (en) 2012-09-04 2019-05-21 Kabushiki Kaisha Dnaform Compound, nucleic acid, labeling substance, and detection method
US20140255928A1 (en) 2013-03-11 2014-09-11 Elitech Holding B.V. Methods for true isothermal strand displacement amplification
EP2997161B1 (de) 2013-05-13 2017-09-27 Elitechgroup B.V. Digitale tröpfchen -pcr mit kurzen sonden mit kleineren rillen
US10266903B2 (en) 2014-12-12 2019-04-23 Elitechgroup, Inc. Methods and compositions for detecting antibiotic resistant bacteria
US9988670B2 (en) 2014-12-12 2018-06-05 Elitechgroup B.V. Methods and compositions for detecting antibiotic resistant bacteria
WO2021080629A1 (en) 2019-10-23 2021-04-29 Elitechgroup, Inc. Methods for true isothermal strand displacement amplification
CN115181049B (zh) * 2022-06-01 2023-12-19 郑州玛特瑞斯生物科技有限公司 一种荧光化合物及其制备方法、荧光修饰核苷酸、测序试剂

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5268486A (en) * 1986-04-18 1993-12-07 Carnegie-Mellon Unversity Method for labeling and detecting materials employing arylsulfonate cyanine dyes
US5569587A (en) * 1986-04-18 1996-10-29 Carnegie Mellon University Method for labeling and detecting materials employing luminescent arysulfonate cyanine dyes
US5627027A (en) * 1986-04-18 1997-05-06 Carnegie Mellon University Cyanine dyes as labeling reagents for detection of biological and other materials by luminescence methods
US4981977A (en) * 1989-06-09 1991-01-01 Carnegie-Mellon University Intermediate for and fluorescent cyanine dyes containing carboxylic acid groups
GB9009980D0 (en) * 1990-05-03 1990-06-27 Amersham Int Plc Phosphoramidite derivatives,their preparation and the use thereof in the incorporation of reporter groups on synthetic oligonucleotides
US5556959A (en) * 1993-01-22 1996-09-17 Pharmacia P-L Biochemicals Inc. Indocarbocyanine-linked phosphoramidites
DE4326466A1 (de) * 1993-08-06 1995-02-09 Boehringer Mannheim Gmbh Infrarot-Farbstoff-markierte Nucleotide und ihre Verwendung in der Nucleinsäure-Detektion
US5808043A (en) * 1995-01-18 1998-09-15 Pharmacia Biotech Inc. Composition for stabilization of labelled nucleoside triphosphates and methods for using same

Also Published As

Publication number Publication date
DE69815342D1 (de) 2003-07-10
CA2294979A1 (en) 1998-12-30
JP2002507203A (ja) 2002-03-05
DE69815342T2 (de) 2004-04-29
EP0989990B1 (de) 2003-06-04
WO1998058942A1 (en) 1998-12-30
US5986086A (en) 1999-11-16
EP0989990A1 (de) 2000-04-05

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