ATE242331T1 - PORPHYROMONAS GINGIVALIS ARGININE-SPECIFIC PROTEINASE CODING SEQUENCES - Google Patents
PORPHYROMONAS GINGIVALIS ARGININE-SPECIFIC PROTEINASE CODING SEQUENCESInfo
- Publication number
- ATE242331T1 ATE242331T1 AT94929183T AT94929183T ATE242331T1 AT E242331 T1 ATE242331 T1 AT E242331T1 AT 94929183 T AT94929183 T AT 94929183T AT 94929183 T AT94929183 T AT 94929183T AT E242331 T1 ATE242331 T1 AT E242331T1
- Authority
- AT
- Austria
- Prior art keywords
- gingipain
- coding sequences
- porphyromonas gingivalis
- specific
- amidolytic
- Prior art date
Links
- 102000035195 Peptidases Human genes 0.000 title 1
- 108091005804 Peptidases Proteins 0.000 title 1
- 241000605862 Porphyromonas gingivalis Species 0.000 title 1
- 235000019833 protease Nutrition 0.000 title 1
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 abstract 2
- 101000727041 Porphyromonas gingivalis Gingipain R1 Proteins 0.000 abstract 2
- 230000003024 amidolytic effect Effects 0.000 abstract 2
- 238000003776 cleavage reaction Methods 0.000 abstract 2
- 230000002797 proteolythic effect Effects 0.000 abstract 2
- 230000007017 scission Effects 0.000 abstract 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 abstract 1
- 108010005843 Cysteine Proteases Proteins 0.000 abstract 1
- 102000005927 Cysteine Proteases Human genes 0.000 abstract 1
- 108091020100 Gingipain Cysteine Endopeptidases Proteins 0.000 abstract 1
- 239000004471 Glycine Substances 0.000 abstract 1
- 241000605894 Porphyromonas Species 0.000 abstract 1
- 125000000637 arginyl group Chemical group N[C@@H](CCCNC(N)=N)C(=O)* 0.000 abstract 1
- 229910052791 calcium Inorganic materials 0.000 abstract 1
- 239000011575 calcium Substances 0.000 abstract 1
- 239000002738 chelating agent Substances 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 238000001502 gel electrophoresis Methods 0.000 abstract 1
- 238000001641 gel filtration chromatography Methods 0.000 abstract 1
- 150000002333 glycines Chemical class 0.000 abstract 1
- 239000003112 inhibitor Substances 0.000 abstract 1
- 125000003588 lysine group Chemical group [H]N([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Cosmetics (AREA)
Abstract
Provided herein is a nucleotide sequence encoding an Arg-specific gingipain named gingipain-1 isolated from Porphyromonas gingivallis, having an apparent molecular mass of 50 kDa as estimated by SDS gel electrophoresis and an apparent molecular mass of 44 kDa as estimated by gel filtration chromatography. Gingipain-1 has amidolytic and proteolytic activity for cleavage after arginine residues and has no amidolytic and/or proteolytic activity for cleavage after lysine residues. Its activity is inhibited cysteine protease group-specific inhibitors and chelating agents. It is stabilized by Calcium and stimulated by glycine-containing peptides and glycine analogues.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/119,361 US5523390A (en) | 1993-09-10 | 1993-09-10 | Porphyromonas gingivalis arginine-specific proteinase |
| US08/141,324 US5475097A (en) | 1993-10-21 | 1993-10-21 | Lysine-specific Porphyromonas gingivalis proteinase |
| US26544194A | 1994-06-24 | 1994-06-24 | |
| PCT/US1994/010283 WO1995007286A1 (en) | 1993-09-10 | 1994-09-09 | Porphyromonas gingivalis arginine-specific proteinase coding sequences |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE242331T1 true ATE242331T1 (en) | 2003-06-15 |
Family
ID=27382295
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT94929183T ATE242331T1 (en) | 1993-09-10 | 1994-09-09 | PORPHYROMONAS GINGIVALIS ARGININE-SPECIFIC PROTEINASE CODING SEQUENCES |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP0717747B1 (en) |
| AT (1) | ATE242331T1 (en) |
| DE (1) | DE69432795T2 (en) |
| WO (1) | WO1995007286A1 (en) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5830710A (en) * | 1988-09-08 | 1998-11-03 | University Of Florida | Cloned porphyromonas gingivalis genes and probes for the detection of periodontal disease |
| US5824791A (en) * | 1988-09-08 | 1998-10-20 | University Of Florida | Cloned porphyromonas gingivalis genes and probes for the detection of periodontal disease |
| AUPN627595A0 (en) * | 1995-10-30 | 1995-11-23 | University Of Melbourne, The | Diagnostics and treatments of periodontal disease |
| CA2249994A1 (en) * | 1996-03-22 | 1997-09-25 | University Of Georgia Research Foundation, Inc. | Immunogenic compositions comprising porphyromonas gingivalis peptides and methods |
| AUPO652897A0 (en) | 1997-04-30 | 1997-05-29 | University Of Melbourne, The | Synthetic peptide constructs for the diagnosis and treatment of periodontitis |
| US8129500B2 (en) | 1997-12-10 | 2012-03-06 | Csl Limited | Porphyromonas gingivalis polypeptides and nucleotides |
| WO2000071682A2 (en) * | 1999-05-20 | 2000-11-30 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Crystal structure data of cysteinprotease gingipain r |
| DE19926728B4 (en) | 1999-06-11 | 2011-08-18 | 3M Espe Ag, 82229 | Use of carrier materials and diagnostically useful additives in imaging methods for intraoral diagnostic purposes |
| AUPQ485999A0 (en) | 1999-12-24 | 2000-02-03 | Csl Limited | P. gingivalis antigenic composition |
| DE10061195B4 (en) | 2000-12-08 | 2004-12-02 | 3M Espe Ag | Use of impression materials for the production of treatment devices |
| EP1799705A4 (en) | 2004-09-23 | 2008-11-05 | Univ Melbourne | Antigen complex for the diagnosis and treatment of porphyromonas gingivalis infection |
| CA2652957A1 (en) | 2006-06-27 | 2008-01-03 | Oral Health Australia Pty Ltd. | Porphyromonas gingivalis polypeptides useful in the prevention of periodontal disease |
| US20100297179A1 (en) | 2007-07-12 | 2010-11-25 | Stuart Geoffrey Dashper | Immunology Treatment for Biofilms |
| WO2009006699A1 (en) | 2007-07-12 | 2009-01-15 | Oral Health Australia Pty Ltd | Biofilm treatment |
| DK2328914T3 (en) | 2008-08-29 | 2017-07-10 | Oral Health Australia Pty Ltd | Prevention, treatment and diagnosis of P. gingivalis infection |
-
1994
- 1994-09-09 WO PCT/US1994/010283 patent/WO1995007286A1/en not_active Ceased
- 1994-09-09 AT AT94929183T patent/ATE242331T1/en not_active IP Right Cessation
- 1994-09-09 EP EP94929183A patent/EP0717747B1/en not_active Expired - Lifetime
- 1994-09-09 DE DE69432795T patent/DE69432795T2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| EP0717747A4 (en) | 1999-01-13 |
| EP0717747B1 (en) | 2003-06-04 |
| WO1995007286A1 (en) | 1995-03-16 |
| EP0717747A1 (en) | 1996-06-26 |
| DE69432795T2 (en) | 2004-04-29 |
| DE69432795D1 (en) | 2003-07-10 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |