ATE245193T1 - Thermostabile dna polymerase - Google Patents

Thermostabile dna polymerase

Info

Publication number
ATE245193T1
ATE245193T1 AT96303880T AT96303880T ATE245193T1 AT E245193 T1 ATE245193 T1 AT E245193T1 AT 96303880 T AT96303880 T AT 96303880T AT 96303880 T AT96303880 T AT 96303880T AT E245193 T1 ATE245193 T1 AT E245193T1
Authority
AT
Austria
Prior art keywords
dna polymerase
polymerase
thermostable dna
thermus
homology
Prior art date
Application number
AT96303880T
Other languages
English (en)
Inventor
Maria Davis
Carl Fuller
Bruce Moffett
John James Cunniff
Original Assignee
Amersham Biosciences Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amersham Biosciences Corp filed Critical Amersham Biosciences Corp
Application granted granted Critical
Publication of ATE245193T1 publication Critical patent/ATE245193T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Pulleys (AREA)
AT96303880T 1995-05-31 1996-05-30 Thermostabile dna polymerase ATE245193T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US45568695A 1995-05-31 1995-05-31

Publications (1)

Publication Number Publication Date
ATE245193T1 true ATE245193T1 (de) 2003-08-15

Family

ID=23809855

Family Applications (1)

Application Number Title Priority Date Filing Date
AT96303880T ATE245193T1 (de) 1995-05-31 1996-05-30 Thermostabile dna polymerase

Country Status (8)

Country Link
US (1) US5885813A (de)
EP (1) EP0745676B1 (de)
JP (1) JP3966555B2 (de)
AT (1) ATE245193T1 (de)
CA (1) CA2222744C (de)
DE (1) DE69629077T2 (de)
GB (1) GB2301590B (de)
WO (1) WO1996038568A1 (de)

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US6818431B1 (en) 1995-10-18 2004-11-16 Shanghai Mendel Dna Center Co. Ltd. DNA polymerase having ability to reduce innate selective discrimination against fluorescent dye-labeled dideoxynucleotides
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US6780591B2 (en) * 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6130045A (en) * 1998-06-11 2000-10-10 Clontech Laboratories, Inc. Thermostable polymerase
DE69936919T2 (de) 1998-06-17 2008-05-15 Ge Healthcare Bio-Sciences Corp. Fy7 polymerase
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US7501245B2 (en) * 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
GB9918150D0 (en) * 1999-08-03 1999-10-06 Univ Sheffield Nuclease variant
ATE299940T1 (de) * 1999-08-21 2005-08-15 Amersham Biosciences Corp Taq dna-polymerase mit einer aminosäuresubstitution in position e681 und entsprechende homologe mit einer verbesserten salztoleranz, nützlich für die sequenzierung schwieriger templatesequenzen mittels geladenen oder ungeladenen terminatoren
DE60027040T2 (de) * 1999-10-29 2006-11-23 Stratagene California, La Jolla Zusammensetzungen und methoden zur verwendung von dna polymerasen
US20040009486A1 (en) 1999-10-29 2004-01-15 Sorge Joseph A. Compositions and methods utilizing DNA polymerases
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
ATE356222T1 (de) 2000-10-06 2007-03-15 Univ Columbia Massives parallelverfahren zur dekodierung von dna und rna
WO2002072892A1 (en) 2001-03-12 2002-09-19 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
EP1436385A4 (de) * 2001-09-14 2005-12-14 Invitrogen Corp Dna-polymerasen und mutanten davon
US20050042629A1 (en) 2002-09-13 2005-02-24 Applera Corporation Thermus scotoductus nucleic acid polymerases
US7148340B2 (en) 2001-11-30 2006-12-12 Applera Corporation Thermus thermophilus nucleic acid polymerases
AU2002346498A1 (en) 2001-11-30 2003-06-17 Applera Corporation Thermus brockianus nucleic acid polymerases
WO2003068991A1 (en) * 2002-02-12 2003-08-21 Applera Corporation Polymerase compositions
EP2194123B1 (de) 2003-03-25 2012-08-22 Stratagene California DNA-Polymerasefusionen und Verwendungen dafür
US20050170367A1 (en) * 2003-06-10 2005-08-04 Quake Stephen R. Fluorescently labeled nucleoside triphosphates and analogs thereof for sequencing nucleic acids
AU2004203649B2 (en) 2003-08-12 2006-01-12 F. Hoffmann-La Roche Ag Thermostable Taq polymerase fragment
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
WO2005054441A2 (en) * 2003-12-01 2005-06-16 California Institute Of Technology Device for immobilizing chemical and biomedical species and methods of using same
US8304189B2 (en) 2003-12-01 2012-11-06 Life Technologies Corporation Nucleic acid molecules containing recombination sites and methods of using the same
EP2248911A1 (de) 2004-02-19 2010-11-10 Helicos Biosciences Corporation Verfahren und Zusammensetzungen zur Analyse von Polynukleotidsequenzen
US20050196392A1 (en) * 2004-02-20 2005-09-08 Andersen Mark R. Lesion repair polymerase compositions
US7417133B2 (en) * 2004-02-27 2008-08-26 Institut Pasteur Methods for obtaining thermostable enzymes, DNA polymerase I variants from Thermus aquaticus having new catalytic activities, methods for obtaining the same, and applications of the same
US20060046258A1 (en) * 2004-02-27 2006-03-02 Lapidus Stanley N Applications of single molecule sequencing
US20050239085A1 (en) * 2004-04-23 2005-10-27 Buzby Philip R Methods for nucleic acid sequence determination
US20050260609A1 (en) * 2004-05-24 2005-11-24 Lapidus Stanley N Methods and devices for sequencing nucleic acids
US20070117104A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
CA2566806A1 (en) * 2004-05-25 2006-01-19 Helicos Biosciences Corporation Methods and devices for nucleic acid sequence determination
US7476734B2 (en) * 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
US20070117103A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US20060024678A1 (en) * 2004-07-28 2006-02-02 Helicos Biosciences Corporation Use of single-stranded nucleic acid binding proteins in sequencing
US20060118754A1 (en) * 2004-12-08 2006-06-08 Lapen Daniel C Stabilizing a polyelectrolyte multilayer
WO2006063355A2 (en) * 2004-12-11 2006-06-15 Cytogenix , Inc. Cell free biosynthesis of high-quality nucleic acid and uses thereof
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US20060172328A1 (en) * 2005-01-05 2006-08-03 Buzby Philip R Methods and compositions for correcting misincorporation in a nucleic acid synthesis reaction
US20060228726A1 (en) 2005-01-06 2006-10-12 Martin Patrick K Polypeptides having nucleic acid binding activity and compositions and methods for nucleic acid amplification
US7482120B2 (en) * 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
US20060263790A1 (en) * 2005-05-20 2006-11-23 Timothy Harris Methods for improving fidelity in a nucleic acid synthesis reaction
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US20070059713A1 (en) 2005-09-09 2007-03-15 Lee Jun E SSB-DNA polymerase fusion proteins
US20070117102A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US20070128610A1 (en) * 2005-12-02 2007-06-07 Buzby Philip R Sample preparation method and apparatus for nucleic acid sequencing
WO2011027966A2 (en) 2009-09-03 2011-03-10 Seegene, Inc. Td probe and its uses
JP5876479B2 (ja) 2010-06-18 2016-03-02 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 増大した３’末端ミスマッチ識別を有するｄｎａポリメラーゼ
US20240102067A1 (en) * 2022-09-26 2024-03-28 Illumina, Inc. Resynthesis Kits and Methods

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WO1991009944A2 (en) * 1989-12-22 1991-07-11 F.Hoffmann-La Roche Ag High temperature reverse transcriptases
US5466591A (en) * 1986-08-22 1995-11-14 Hoffmann-La Roche Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US4889818A (en) * 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US4795699A (en) * 1987-01-14 1989-01-03 President And Fellows Of Harvard College T7 DNA polymerase
US5075216A (en) * 1988-09-23 1991-12-24 Cetus Corporation Methods for dna sequencing with thermus aquaticus dna polymerase
CA2050276C (en) * 1989-04-12 2003-03-11 Stanley Tabor Improved primer extension reactions
EP0894860B1 (de) * 1990-09-28 2012-12-12 F. Hoffmann-La Roche AG Thermostabile DNA Polymerasen und deren Deletionsmutanten
AU8906091A (en) * 1990-10-05 1992-04-28 Wayne M. Barnes Thermostable dna polymerase
FI923911A7 (fi) * 1992-09-01 1994-03-02 Timo Korpela DNA-molekyylien in vitro-synteesi
US5436149A (en) * 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US5474920A (en) * 1993-11-23 1995-12-12 State Of Oregon, Acting By And Through The Oregon State Board Of Higher Education On Behalf Of The Oregon Health Sciences University Modified thermo-resistant DNA polymerases
EP0655506B1 (de) * 1994-10-17 1996-09-18 President And Fellows Of Harvard College DNS Polymerase mit veränderter Nukleotid-Bindungstelle

Also Published As

Publication number Publication date
GB9611218D0 (en) 1996-07-31
DE69629077T2 (de) 2004-04-22
WO1996038568A1 (en) 1996-12-05
JPH11507211A (ja) 1999-06-29
DE69629077D1 (de) 2003-08-21
CA2222744C (en) 2008-03-25
EP0745676A1 (de) 1996-12-04
EP0745676B1 (de) 2003-07-16
US5885813A (en) 1999-03-23
JP3966555B2 (ja) 2007-08-29
CA2222744A1 (en) 1996-12-05
GB2301590B (en) 1997-07-09
GB2301590A (en) 1996-12-11

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