ATE247127T1 - System und verfahren zur durchführung von polynukleotid-trennungen mit hilfe von flüssigchromatographie - Google Patents
System und verfahren zur durchführung von polynukleotid-trennungen mit hilfe von flüssigchromatographieInfo
- Publication number
- ATE247127T1 ATE247127T1 AT98923484T AT98923484T ATE247127T1 AT E247127 T1 ATE247127 T1 AT E247127T1 AT 98923484 T AT98923484 T AT 98923484T AT 98923484 T AT98923484 T AT 98923484T AT E247127 T1 ATE247127 T1 AT E247127T1
- Authority
- AT
- Austria
- Prior art keywords
- chromatography
- polynucleotide
- guard
- multivalent
- components
- Prior art date
Links
- 102000040430 polynucleotide Human genes 0.000 title abstract 6
- 108091033319 polynucleotide Proteins 0.000 title abstract 6
- 239000002157 polynucleotide Substances 0.000 title abstract 6
- 238000004811 liquid chromatography Methods 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 238000004587 chromatography analysis Methods 0.000 abstract 4
- 150000001768 cations Chemical class 0.000 abstract 3
- 239000012634 fragment Substances 0.000 abstract 3
- 150000002500 ions Chemical class 0.000 abstract 3
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 abstract 2
- 239000000463 material Substances 0.000 abstract 2
- 239000011347 resin Substances 0.000 abstract 2
- 229920005989 resin Polymers 0.000 abstract 2
- 238000000926 separation method Methods 0.000 abstract 2
- 229910001220 stainless steel Inorganic materials 0.000 abstract 2
- 239000010935 stainless steel Substances 0.000 abstract 2
- 229910052719 titanium Inorganic materials 0.000 abstract 2
- 239000010936 titanium Substances 0.000 abstract 2
- 239000007864 aqueous solution Substances 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 238000011144 upstream manufacturing Methods 0.000 abstract 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
- B01D15/366—Ion-pair, e.g. ion-pair reversed phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US4912397P | 1997-06-10 | 1997-06-10 | |
| US6383597P | 1997-10-30 | 1997-10-30 | |
| US7852398P | 1998-03-18 | 1998-03-18 | |
| PCT/US1998/010122 WO1998056797A1 (en) | 1997-06-10 | 1998-05-19 | System and method for performing polynucleotide separations using liquid chromatography |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE247127T1 true ATE247127T1 (de) | 2003-08-15 |
Family
ID=27367483
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT98923484T ATE247127T1 (de) | 1997-06-10 | 1998-05-19 | System und verfahren zur durchführung von polynukleotid-trennungen mit hilfe von flüssigchromatographie |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US6491821B2 (de) |
| EP (1) | EP1023306B1 (de) |
| JP (1) | JP2000516638A (de) |
| AT (1) | ATE247127T1 (de) |
| AU (1) | AU731812B2 (de) |
| CA (1) | CA2288603A1 (de) |
| DE (1) | DE69817207T2 (de) |
| WO (1) | WO1998056797A1 (de) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6576133B2 (en) | 1996-11-13 | 2003-06-10 | Transgenomic, Inc | Method and system for RNA analysis by matched ion polynucleotide chromatography |
| US6475388B1 (en) | 1996-11-13 | 2002-11-05 | Transgenomic, Inc. | Method and system for RNA analysis by matched ion polynucleotide chromatography |
| US6177559B1 (en) | 1998-04-24 | 2001-01-23 | Transgenomic, Inc. | Process for separation of polynucleotide fragments |
| US6258264B1 (en) | 1998-04-10 | 2001-07-10 | Transgenomic, Inc. | Non-polar media for polynucleotide separations |
| US6265168B1 (en) | 1998-10-06 | 2001-07-24 | Transgenomic, Inc. | Apparatus and method for separating and purifying polynucleotides |
| US6074880A (en) | 1998-08-28 | 2000-06-13 | Transgenomic, Inc. | Sample analyte containing solution fraction collection system, and method of use |
| US6238565B1 (en) * | 1998-09-16 | 2001-05-29 | Varian, Inc. | Monolithic matrix for separating bio-organic molecules |
| EP1231993A4 (de) * | 1999-09-17 | 2003-04-16 | Transgenomic Inc | Verbesserte kolonne zur dna-trennung durch polynukleotidchromatogrphie mit aufeinander abgestimmten ionen |
| CA2395101A1 (en) * | 2000-05-01 | 2001-11-08 | Transgenomic, Inc. | Polynucleotide separations on polymeric separation media |
| WO2001092510A1 (en) * | 2000-05-26 | 2001-12-06 | Transgenomic, Inc. | Apparatus and method for separating and purifying polynucleotides |
| US6521411B2 (en) | 2000-09-28 | 2003-02-18 | Transgenomic, Inc. | Method and system for the preparation of cDNA |
| AU2002334892A1 (en) | 2001-10-05 | 2003-04-22 | Transgenomic, Inc. | Methods and compositions for mutation analysis by liquid chromatography |
| WO2004024285A1 (en) | 2002-09-10 | 2004-03-25 | Waters Investments Limited | A chromatographic column and methods for controlling sorbent density |
| US20070084982A1 (en) * | 2005-10-17 | 2007-04-19 | Agilent Technologies, Inc. | Holding device for chromatograph columns |
| US8501029B2 (en) * | 2006-10-30 | 2013-08-06 | Agilent Technologies, Inc. | Micromachined titanium for high pressure microfluidic applications |
| US20100207051A1 (en) * | 2006-12-21 | 2010-08-19 | Invitrogen Dynal As | Particles and their use in a method for isolating nucleic acid or a method for isolating phosphoproteins |
| WO2009023268A1 (en) * | 2007-08-16 | 2009-02-19 | Alltech Associates Inc. | Chromatography column assemblies |
| US11912983B2 (en) | 2019-12-05 | 2024-02-27 | Waters Technologies Corporation | Polyanionic acids to improve recovery and minimize system loss |
| CN113109497B (zh) * | 2021-05-10 | 2022-06-28 | 青岛盛瀚色谱技术有限公司 | 一种离子对色谱法测定6-溴己基三甲基溴化铵中相关物质的方法 |
Family Cites Families (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NO145164C (no) | 1978-11-06 | 1982-01-27 | Sintef | Fremgangsmaate for fremstilling av polymerlateks. |
| US4407199A (en) * | 1980-10-06 | 1983-10-04 | Mosstype Corporation | Mandrel assembly for demountable printing cylinders of different lengths |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4855225A (en) | 1986-02-07 | 1989-08-08 | Applied Biosystems, Inc. | Method of detecting electrophoretically separated oligonucleotides |
| US5227059A (en) | 1989-11-08 | 1993-07-13 | Alltech Associates, Inc. | Chromatography columns |
| CA2063855C (en) | 1991-04-03 | 1997-08-26 | Will Bloch | Precision and accuracy of anion-exchange separation of nucleic acids |
| US5338448A (en) | 1992-10-16 | 1994-08-16 | Sarasep, Inc. | Method of preventing contamination of a chromatography column |
| AT398973B (de) * | 1992-11-18 | 1995-02-27 | Bonn Guenther Dr | Verfahren zur trennung von nukleinsäuren |
| US5438127A (en) | 1993-09-27 | 1995-08-01 | Becton Dickinson And Company | DNA purification by solid phase extraction using a PCl3 modified glass fiber membrane |
| US5651931A (en) | 1994-01-27 | 1997-07-29 | Upchurch Scientific, Inc. | Method of making a biocompatible filter |
| US5705628A (en) | 1994-09-20 | 1998-01-06 | Whitehead Institute For Biomedical Research | DNA purification and isolation using magnetic particles |
| US5772889A (en) * | 1995-11-13 | 1998-06-30 | Transgenomic, Inc. | System and method for performing nucleic acid separations using liquid chromatography |
| US6174441B1 (en) * | 1996-11-13 | 2001-01-16 | Transgenomic, Inc. | Method for performing polynucleotide separations using liquid chromatography |
| US6030527A (en) * | 1996-11-13 | 2000-02-29 | Transgenomic, Inc. | Apparatus for performing polynucleotide separations using liquid chromatography |
| US6017457A (en) * | 1996-11-13 | 2000-01-25 | Transgenomic, Inc. | Method for performing polynucleotide separations using liquid chromatography |
| US5972222A (en) * | 1996-11-13 | 1999-10-26 | Transgenomic, Inc. | Process for performing polynucleotide separations |
| US5997742A (en) * | 1996-11-13 | 1999-12-07 | Transgenomic, Inc. | Method for performing polynucleotide separations using liquid chromatography |
| US6024878A (en) * | 1997-10-30 | 2000-02-15 | Transgenomic, Inc. | Method for high resolution liquid chromatographic separation of polynucleotides |
| US6136195A (en) * | 1998-04-10 | 2000-10-24 | Transgenomic, Inc. | MIPC column cleaning system and process |
-
1998
- 1998-05-19 AT AT98923484T patent/ATE247127T1/de not_active IP Right Cessation
- 1998-05-19 JP JP11502475A patent/JP2000516638A/ja active Pending
- 1998-05-19 EP EP98923484A patent/EP1023306B1/de not_active Expired - Lifetime
- 1998-05-19 WO PCT/US1998/010122 patent/WO1998056797A1/en not_active Ceased
- 1998-05-19 AU AU75772/98A patent/AU731812B2/en not_active Ceased
- 1998-05-19 DE DE69817207T patent/DE69817207T2/de not_active Expired - Lifetime
- 1998-05-19 CA CA002288603A patent/CA2288603A1/en not_active Abandoned
-
2001
- 2001-04-05 US US09/828,346 patent/US6491821B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EP1023306A4 (de) | 2002-01-16 |
| JP2000516638A (ja) | 2000-12-12 |
| AU731812B2 (en) | 2001-04-05 |
| DE69817207T2 (de) | 2004-06-17 |
| CA2288603A1 (en) | 1998-12-17 |
| US20010047961A1 (en) | 2001-12-06 |
| EP1023306B1 (de) | 2003-08-13 |
| US6491821B2 (en) | 2002-12-10 |
| DE69817207D1 (de) | 2003-09-18 |
| AU7577298A (en) | 1998-12-30 |
| WO1998056797A1 (en) | 1998-12-17 |
| EP1023306A1 (de) | 2000-08-02 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |