ATE248631T1 - Einstufige vorrichtung und verfahren zur konzentration und säuberung biologischer moleküle - Google Patents

Einstufige vorrichtung und verfahren zur konzentration und säuberung biologischer moleküle

Info

Publication number
ATE248631T1
ATE248631T1 AT98930494T AT98930494T ATE248631T1 AT E248631 T1 ATE248631 T1 AT E248631T1 AT 98930494 T AT98930494 T AT 98930494T AT 98930494 T AT98930494 T AT 98930494T AT E248631 T1 ATE248631 T1 AT E248631T1
Authority
AT
Austria
Prior art keywords
purification
biological molecules
concentration
step device
known volume
Prior art date
Application number
AT98930494T
Other languages
English (en)
Inventor
Jonathan L Coffman
Original Assignee
Life Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Inc filed Critical Life Technologies Inc
Application granted granted Critical
Publication of ATE248631T1 publication Critical patent/ATE248631T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1864—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns
    • B01D15/1871—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns placed in series
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/34—Size-selective separation, e.g. size-exclusion chromatography; Gel filtration; Permeation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
AT98930494T 1997-06-27 1998-06-24 Einstufige vorrichtung und verfahren zur konzentration und säuberung biologischer moleküle ATE248631T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US5175697P 1997-06-27 1997-06-27
PCT/US1998/013163 WO1999000168A1 (en) 1997-06-27 1998-06-24 One step device and process for concentration and purification of biological molecules

Publications (1)

Publication Number Publication Date
ATE248631T1 true ATE248631T1 (de) 2003-09-15

Family

ID=21973201

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98930494T ATE248631T1 (de) 1997-06-27 1998-06-24 Einstufige vorrichtung und verfahren zur konzentration und säuberung biologischer moleküle

Country Status (8)

Country Link
US (1) US5981736A (de)
EP (1) EP0991456B1 (de)
JP (1) JP2002511147A (de)
AT (1) ATE248631T1 (de)
AU (1) AU7987398A (de)
CA (1) CA2294749C (de)
DE (1) DE69817818T2 (de)
WO (1) WO1999000168A1 (de)

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AU9786798A (en) * 1997-10-10 1999-05-03 Biosepra Inc. Aligned multiwell multiplate stack and method for processing biological/chemicalsamples using the same
DE19859703B4 (de) * 1998-12-23 2009-10-29 Macherey, Nagel Gmbh & Co. Handelsgesellschaft Verfahren zur Aufreinigung von Nukleinsäuren sowie Anionenaustauscher zur Durchführung dieses Verfahrens
ATE278699T1 (de) * 2000-02-16 2004-10-15 Macherey Nagel Gmbh & Co Hg Verfahren zur aufreinigung von nukleinsäuren sowie anionenaustauscher zur durchführung dieses verfahrens
US20080260593A1 (en) * 2000-03-22 2008-10-23 Dewalch Norman Binz Method and apparatus for processing substances in a single container
CA2495797C (en) 2002-08-20 2013-12-17 B.R.A.I.N. Biotechnology Research And Information Network Ag Isolation and cloning of dna from uncultivated organisms
SE0300975D0 (sv) * 2003-04-03 2003-04-03 Protista Internat Ab Chromatographic separation method, separation device and process for the preparation of a separation medium for use therein
US7943393B2 (en) * 2003-07-14 2011-05-17 Phynexus, Inc. Method and device for extracting an analyte
US7240572B2 (en) * 2004-02-18 2007-07-10 Millipore Corporation Vacuum assisted affinity chromatography device and method
CN100545653C (zh) * 2004-07-29 2009-09-30 兴和株式会社 铅测定用试剂
DE102007005655A1 (de) * 2007-01-31 2008-08-07 Qiagen Gmbh Vorrichtung und Verfahren zur Aufreinigung von Nukleinsäuren
WO2009016431A1 (en) * 2007-08-01 2009-02-05 Digilab, Inc. Sample preparation method and apparatus
EP2638156B1 (de) 2010-11-09 2016-01-27 Qiagen GmbH Verfahren und vorrichtung zur isolierung und reinigung von doppelsträngigen nukleinsäuren
KR102654881B1 (ko) * 2023-06-23 2024-04-17 주식회사 진시스템 핵산 분리 장치 및 방법

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US3433782A (en) * 1965-12-27 1969-03-18 Miles Lab Separation and recovery of oligonucleotides
BE757291A (fr) * 1969-10-10 1971-04-09 Smith Kline French Lab Complexe antiviral de arn et d'un polysaccharide
JPS5312900A (en) * 1976-07-23 1978-02-04 Yamasa Shoyu Co Ltd Extraction of natural ribonucleic acid
SU638599A1 (ru) * 1977-07-11 1978-12-25 Московский Ордена Ленина И Ордена Трудового Красного Знамени Государственный Университет Им. М.В.Ломоносова Способ получени фракций нуклеиновых кислот
US4283199A (en) * 1979-08-20 1981-08-11 Forsyth Dental Infirmary For Children Method of resolving biological solutions
DE3044385A1 (de) * 1980-11-25 1982-06-24 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren zur durchfuehrung analytischer bestimmungen und hierfuer geeignetes rotoreinsatzelement
US4830969A (en) * 1981-08-31 1989-05-16 The Research Foundation Of State University Of New York Process for the rapid and simple isolation of nucleic acids
DE3211309A1 (de) * 1982-03-26 1983-09-29 Metin Dipl.-Ing. 6100 Darmstadt Colpan Chromatographisches verfahren zur isolierung von makromolekuelen
IL66551A (en) * 1982-08-15 1985-11-29 Cais Michael Method for moving-bed chromatography and device therefor
DE3308932A1 (de) * 1983-03-12 1984-09-13 Hoechst Ag, 6230 Frankfurt Verfahren zur abtrennung von ribonukleinsaeuren aus einer loesung, die desoxyribonukleinsaeuren enthaelt
JPS60227166A (ja) * 1984-08-08 1985-11-12 Daicel Chem Ind Ltd 液体クロマトグラフイ−用カラム
US4719011A (en) * 1985-03-22 1988-01-12 H. T. Chemicals, Inc. High pressure liquid chromatography columns
DE3639949A1 (de) * 1986-11-22 1988-06-09 Diagen Inst Molekularbio Verfahren zur trennung von langkettigen nukleinsaeuren
US4935342A (en) * 1986-12-01 1990-06-19 Syngene, Inc. Method of isolating and purifying nucleic acids from biological samples
US4923978A (en) * 1987-12-28 1990-05-08 E. I. Du Pont De Nemours & Company Process for purifying nucleic acids
US4810381A (en) * 1987-12-28 1989-03-07 Minnesota Mining And Manufacturing Company Composite chromatographic article
US4921952A (en) * 1988-01-21 1990-05-01 The United States Of America As Represented By The United States Department Of Energy Nucleic acid isolation process
US5075430A (en) * 1988-12-12 1991-12-24 Bio-Rad Laboratories, Inc. Process for the purification of DNA on diatomaceous earth
IT1226210B (it) * 1988-12-22 1990-12-21 Claudio Schneider Procedimento per l'estrazione e la purificazione di dna
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US4935142A (en) * 1989-06-23 1990-06-19 Memtek Corporation Apparatus for retaining variable number of sheet membrane elements and a method for the use thereof
DE3935098C2 (de) * 1989-10-21 1995-05-24 Macherey Nagel & Co Chem Chromatographisches Trägermaterial sowie seine Verwendung in einem Verfahren zur chromatographischen Trennung von Nukleinsäuren
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Also Published As

Publication number Publication date
US5981736A (en) 1999-11-09
AU7987398A (en) 1999-01-19
CA2294749C (en) 2007-01-02
CA2294749A1 (en) 1999-01-07
DE69817818T2 (de) 2004-07-15
DE69817818D1 (de) 2003-10-09
JP2002511147A (ja) 2002-04-09
WO1999000168A1 (en) 1999-01-07
EP0991456B1 (de) 2003-09-03
EP0991456A1 (de) 2000-04-12

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