ATE254648T1 - Rot-emittierende (8,9)benzophenoxazin- nukleinsäurefarbstoffe und verfahren zu deren verwendung - Google Patents

Rot-emittierende (8,9)benzophenoxazin- nukleinsäurefarbstoffe und verfahren zu deren verwendung

Info

Publication number
ATE254648T1
ATE254648T1 AT00959749T AT00959749T ATE254648T1 AT E254648 T1 ATE254648 T1 AT E254648T1 AT 00959749 T AT00959749 T AT 00959749T AT 00959749 T AT00959749 T AT 00959749T AT E254648 T1 ATE254648 T1 AT E254648T1
Authority
AT
Austria
Prior art keywords
emitting
red
nucleic acid
benzophenoxazine
acid dyes
Prior art date
Application number
AT00959749T
Other languages
English (en)
Inventor
Xiongwui Yan
Sheri Miraglia
Pau Miau Yuan
Original Assignee
Applera Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applera Corp filed Critical Applera Corp
Application granted granted Critical
Publication of ATE254648T1 publication Critical patent/ATE254648T1/de

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07DHETEROCYCLIC COMPOUNDS
    • C07D265/00Heterocyclic compounds containing six-membered rings having one nitrogen atom and one oxygen atom as the only ring hetero atoms
    • C07D265/281,4-Oxazines; Hydrogenated 1,4-oxazines
    • C07D265/341,4-Oxazines; Hydrogenated 1,4-oxazines condensed with carbocyclic rings
    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09BORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B19/00Oxazine dyes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Nitrogen And Oxygen As The Only Ring Hetero Atoms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Coloring (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
  • Plural Heterocyclic Compounds (AREA)
AT00959749T 1999-09-03 2000-09-01 Rot-emittierende (8,9)benzophenoxazin- nukleinsäurefarbstoffe und verfahren zu deren verwendung ATE254648T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/389,918 US6140500A (en) 1999-09-03 1999-09-03 Red-emitting [8,9]benzophenoxazine nucleic acid dyes and methods for their use
PCT/US2000/024057 WO2001018124A1 (en) 1999-09-03 2000-09-01 Red-emitting [8,9]benzophenoxazine nucleic acid dyes and methods for their use

Publications (1)

Publication Number Publication Date
ATE254648T1 true ATE254648T1 (de) 2003-12-15

Family

ID=23540303

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00959749T ATE254648T1 (de) 1999-09-03 2000-09-01 Rot-emittierende (8,9)benzophenoxazin- nukleinsäurefarbstoffe und verfahren zu deren verwendung

Country Status (8)

Country Link
US (1) US6140500A (de)
EP (1) EP1208160B1 (de)
JP (1) JP2003509528A (de)
AT (1) ATE254648T1 (de)
AU (1) AU753608B2 (de)
CA (1) CA2382593C (de)
DE (1) DE60006685T2 (de)
WO (1) WO2001018124A1 (de)

Families Citing this family (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6465644B1 (en) 2000-05-02 2002-10-15 Applera Corporation Sulfonated [8,9] benzophenoxazine dyes and the use of their labelled conjugates
CA2417816A1 (en) 2000-08-04 2002-02-14 Molecular Probes, Inc. Derivatives of 1,2-dihydro-7-hydroxyquinolines containing fused rings
US7169922B2 (en) 2000-08-04 2007-01-30 Invitrogen Corporation Derivatives of 1,2-dihydro-7-hydroxyquinolines containing fused rings
EP1317511B1 (de) * 2000-09-06 2005-01-19 Evotec OAI AG Oxazinderivate
EP1213328A1 (de) * 2000-12-08 2002-06-12 Evotec OAI AG Oxazinderivate
DE60216468T2 (de) 2001-03-09 2007-09-27 Boston Probes, Inc., Bedford Kombinationsoligomere betreffende verfahren, kits und zusammensetzungen
EP1448800A4 (de) 2001-11-21 2007-05-16 Applera Corp Digitaler assay
WO2004022578A2 (en) * 2002-09-08 2004-03-18 Applera Corporation Methods, compositions and libraries pertaining pna dimer and pna oligomer synthesis
US7570443B2 (en) 2003-09-19 2009-08-04 Applied Biosystems, Llc Optical camera alignment
US7417726B2 (en) * 2003-09-19 2008-08-26 Applied Biosystems Inc. Normalization of data using controls
US20060029948A1 (en) * 2003-09-19 2006-02-09 Gary Lim Sealing cover and dye compatibility selection
ATE419263T1 (de) * 2003-11-20 2009-01-15 Exiqon As Löscher-zusammensetzung mit anthraquinon
WO2005077125A2 (en) * 2004-02-11 2005-08-25 Applera Corporation Methods and compositions for detecting nucleic acids
US7473529B1 (en) * 2004-03-31 2009-01-06 Iowa State University Research Foundation, Inc. System and method for detecting cells or components thereof
US20050255485A1 (en) * 2004-05-14 2005-11-17 Livak Kenneth J Detection of gene duplications
EP2290072B1 (de) 2004-05-28 2014-12-17 Asuragen, Inc. Verfahren und Zusammensetzungen mit MicroRNA
WO2006016978A1 (en) * 2004-06-30 2006-02-16 Applera Corporation Analog probe complexes
ES2534304T3 (es) 2004-11-12 2015-04-21 Asuragen, Inc. Procedimientos y composiciones que implican miARN y moléculas inhibidoras de miARN
US20060292586A1 (en) * 2004-12-17 2006-12-28 Schroth Gary P ID-tag complexes, arrays, and methods of use thereof
US20060166238A1 (en) * 2004-12-22 2006-07-27 Ramsing Niels B Probes, libraries and kits for analysis of mixtures of nucleic acids and methods for constructing the same
EP2208796B1 (de) 2005-01-06 2014-06-18 Applied Biosystems, LLC Polypeptide mit Nukleinsäurebindungsaktivität und Zusammensetzungen und Verfahren zur Nukleinsäurenamplifikation
EP2312317A3 (de) 2005-05-03 2011-06-08 Life Technologies Corporation Fluoreszentes Erkennungssystem und Farbstoffsatz zur Verwendung damit
US8362250B2 (en) 2005-05-24 2013-01-29 Enzo Biochem, Inc. Fluorescent dyes and compounds, methods and kits useful for identifying specific organelles and regions in cells of interest
US20070087360A1 (en) * 2005-06-20 2007-04-19 Boyd Victoria L Methods and compositions for detecting nucleotides
US20070059713A1 (en) 2005-09-09 2007-03-15 Lee Jun E SSB-DNA polymerase fusion proteins
EP1994182B1 (de) 2006-03-15 2019-05-29 Siemens Healthcare Diagnostics Inc. Abgleit-nucleobase-analoga
US20080124726A1 (en) 2006-05-26 2008-05-29 Althea Technologies, Inc. Biochemical analysis of partitioned cells
US20080131878A1 (en) * 2006-12-05 2008-06-05 Asuragen, Inc. Compositions and Methods for the Detection of Small RNA
CA2671194A1 (en) * 2006-12-08 2008-06-19 Asuragen, Inc. Mir-20 regulated genes and pathways as targets for therapeutic intervention
US20080274458A1 (en) * 2007-05-01 2008-11-06 Latham Gary J Nucleic acid quantitation methods
EP2198050A1 (de) 2007-09-14 2010-06-23 Asuragen, INC. Mikrornas mit unterschiedlicher expression bei zervikalkarzinom und verwendungen davon
US8071562B2 (en) 2007-12-01 2011-12-06 Mirna Therapeutics, Inc. MiR-124 regulated genes and pathways as targets for therapeutic intervention
US8258111B2 (en) 2008-05-08 2012-09-04 The Johns Hopkins University Compositions and methods related to miRNA modulation of neovascularization or angiogenesis
US8715944B2 (en) * 2008-09-08 2014-05-06 Enzo Life Sciences, Inc. Fluorochromes for organelle tracing and multi-color imaging
US9250249B2 (en) 2008-09-08 2016-02-02 Enzo Biochem, Inc. Autophagy and phospholipidosis pathway assays
US9334281B2 (en) * 2008-09-08 2016-05-10 Enzo Life Sciences, Inc. Fluorochromes for organelle tracing and multi-color imaging
EP3597657B1 (de) 2009-05-21 2021-09-01 Siemens Healthcare Diagnostics Inc. Universaletiketten mit nichtnatürlichen nukleobasen
AU2010249421A1 (en) 2009-05-22 2011-12-08 Asuragen, Inc. miRNA biomarkers of prostate disease
EP2470897A4 (de) 2009-08-28 2013-05-29 Asuragen Inc Mirna-biomarker für lungenerkrankungen
EP2633073B1 (de) 2010-10-29 2017-04-26 Asuragen, Inc. Mpcr verfahren zur analyse von wiederholungssequenzen
WO2012092403A1 (en) 2010-12-29 2012-07-05 Life Technologies Corporation Ddao compounds as fluorescent reference standards
US9644241B2 (en) 2011-09-13 2017-05-09 Interpace Diagnostics, Llc Methods and compositions involving miR-135B for distinguishing pancreatic cancer from benign pancreatic disease
WO2014093291A1 (en) 2012-12-10 2014-06-19 Advandx, Inc. Use of probes for mass spectrometric identification of microorganisms or cells and associated conditions of interest
EP3425066A1 (de) 2014-01-05 2019-01-09 Biomirna Holdings Ltd. Lungenkrebsbestimmung mit mirna
WO2015120382A1 (en) 2014-02-07 2015-08-13 The Johns Hopkins University Predicting response to epigenetic drug therapy
EP3183054B1 (de) 2014-08-19 2020-09-23 Roche Diagnostics GmbH Verfahren und zusammensetzungen zum nachweis von nukleinsäuren
WO2016094330A2 (en) 2014-12-08 2016-06-16 20/20 Genesystems, Inc Methods and machine learning systems for predicting the liklihood or risk of having cancer
EP3292214A1 (de) 2015-05-04 2018-03-14 Academisch Medisch Centrum Verfahren zur quantifizierung von mirnas mittels normalisierung
US11214829B2 (en) 2015-07-23 2022-01-04 Asuragen, Inc. Methods, compositions, kits, and uses for analysis of nucleic acids comprising repeating A/T-rich segments
WO2018045162A1 (en) 2016-09-01 2018-03-08 Biogen Ma Inc. Biomarkers predictive of primary progressive multiple sclerosis and uses thereof
CN107602498B (zh) * 2017-07-26 2020-07-28 华南理工大学 一种荧光探针及其制备方法与在酪氨酸酶检测中的应用
WO2019193544A1 (en) * 2018-04-04 2019-10-10 Universidade Do Minho Benzophenoxazine derivatives for diagnosis of neoplasia or infection
WO2020090294A1 (ja) * 2018-10-31 2020-05-07 日立化成株式会社 イオン性化合物、有機エレクトロニクス材料、インク組成物、及び有機エレクトロニクス素子
EP3719144A1 (de) 2019-04-05 2020-10-07 Fundación para la Investigación Biomédica del Hospital Universitario de la Paz (FIBHULP) Mir-151a-3p als universelle endogene steuerung zur exosomenladungsnormalisierung
EP3999664A1 (de) 2019-07-19 2022-05-25 Fundación para la Investigación Biomédica del Hospital Universitario de la Paz (FIBHULP) Verfahren zur bestimmung des ansprechens auf die behandlung eines patienten mit nicht-kleinzelligem lungenkarzinom (nsclc)
EP4367104A4 (de) * 2021-07-02 2025-11-19 Univ Oregon Health & Science Wasserlösliche nervenfarbstoffe zur nervenspezifischen in-vivo-bildgebung
KR102783485B1 (ko) 2021-10-15 2025-03-20 아주대학교산학협력단 miR-625-3p의 건선 중증도 진단용 바이오마커로서의 용도
KR20240177780A (ko) 2023-06-19 2024-12-30 재단법인 아산사회복지재단 맥락막 비후 질환의 진단 또는 예후 예측용 조성물, 키트 및 진단 또는 예후 예측을 위한 정보를 제공하는 방법

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE488945C (de) * 1924-09-12 1930-01-25 I G Farbenindustrie Akt Ges Verfahren zur Darstellung von Aminoalkylaminosubstitutionsprodukten der Azin-, Oxazin- und Thiazinreihe
US4386149A (en) * 1978-02-28 1983-05-31 Ciba-Geigy Ag Process for the production of photographic images by dye diffusion transfer and photographic material suitable in this process
US4237281A (en) * 1978-10-13 1980-12-02 Ciba-Geigy Aktiengesellschaft Dyestuffs containing amino or imino groups
US4789742A (en) * 1984-08-27 1988-12-06 Spyros Theodoropulos Isomaleimide and isophthalimide derivatives of chromophors
US4622395A (en) * 1984-10-01 1986-11-11 Minnesota Mining And Manufacturing Company Phenoxazine and phenothiazine dyes and leuco forms thereof
US4714763A (en) * 1985-07-11 1987-12-22 Viomedics Inc. Novel oxazine-ureas and thiazine urea chromophors as fluorescent labels
US4962197A (en) * 1988-02-12 1990-10-09 Rowland Institute For Science Photo-inactivation of cancer cells
US5783687A (en) * 1990-03-14 1998-07-21 The Regents Of The University Of California Dyes designed for high sensitivity detection of double-stranded DNA
DE59309805D1 (de) * 1992-12-15 1999-11-04 Ciba Sc Holding Ag Oxazinfarbstoffe, Verfahren zu deren Herstellung und deren Verwendung
US5410030A (en) * 1993-04-05 1995-04-25 Molecular Probes, Inc. Dimers of unsymmetrical cyanine dyes containing pyridinium moieties
US5436134A (en) * 1993-04-13 1995-07-25 Molecular Probes, Inc. Cyclic-substituted unsymmetrical cyanine dyes
US5565554A (en) * 1994-07-29 1996-10-15 The Regents Of The University Of California Dimeric fluorescent energy transfer dyes comprising asymmetric cyanine azole-indolenine chromophores
GB9602265D0 (en) * 1996-02-05 1996-04-03 Amersham Int Plc Benzophenoxazine dyes
ID17684A (id) * 1996-02-08 1998-01-22 Bpb Plc Papan bersemen
US5876946A (en) * 1997-06-03 1999-03-02 Pharmacopeia, Inc. High-throughput assay

Also Published As

Publication number Publication date
AU753608B2 (en) 2002-10-24
DE60006685T2 (de) 2004-10-14
CA2382593C (en) 2008-04-08
JP2003509528A (ja) 2003-03-11
AU7101500A (en) 2001-04-10
WO2001018124A1 (en) 2001-03-15
EP1208160A1 (de) 2002-05-29
US6140500A (en) 2000-10-31
CA2382593A1 (en) 2001-03-15
DE60006685D1 (de) 2003-12-24
EP1208160B1 (de) 2003-11-19

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