ATE266671T1 - Nukleinsäuresynthese unter verwendung von mittels licht abspaltbaren schutzgruppen - Google Patents

Nukleinsäuresynthese unter verwendung von mittels licht abspaltbaren schutzgruppen

Info

Publication number
ATE266671T1
ATE266671T1 AT96911307T AT96911307T ATE266671T1 AT E266671 T1 ATE266671 T1 AT E266671T1 AT 96911307 T AT96911307 T AT 96911307T AT 96911307 T AT96911307 T AT 96911307T AT E266671 T1 ATE266671 T1 AT E266671T1
Authority
AT
Austria
Prior art keywords
nucleic acid
acid synthesis
cleared
light
protection groups
Prior art date
Application number
AT96911307T
Other languages
English (en)
Inventor
Michael C Dr Pirrung
Steven W Shuey
Jean-Claude Bradley
Original Assignee
Univ Duke
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Duke filed Critical Univ Duke
Application granted granted Critical
Publication of ATE266671T1 publication Critical patent/ATE266671T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Saccharide Compounds (AREA)
  • Carbon And Carbon Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Silicon Polymers (AREA)
  • Luminescent Compositions (AREA)
AT96911307T 1995-03-16 1996-03-15 Nukleinsäuresynthese unter verwendung von mittels licht abspaltbaren schutzgruppen ATE266671T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/406,327 US5908926A (en) 1995-03-16 1995-03-16 5'to 3' nucleic acid synthesis using 3'-photoremovable protecting group
PCT/US1996/003496 WO1996028457A1 (en) 1995-03-16 1996-03-15 Nucleic acid synthesis using photoremovable protecting groups

Publications (1)

Publication Number Publication Date
ATE266671T1 true ATE266671T1 (de) 2004-05-15

Family

ID=23607492

Family Applications (1)

Application Number Title Priority Date Filing Date
AT96911307T ATE266671T1 (de) 1995-03-16 1996-03-15 Nukleinsäuresynthese unter verwendung von mittels licht abspaltbaren schutzgruppen

Country Status (9)

Country Link
US (1) US5908926A (de)
EP (1) EP0815114B1 (de)
JP (1) JPH11501936A (de)
AT (1) ATE266671T1 (de)
AU (1) AU5422896A (de)
CA (1) CA2215657A1 (de)
DE (1) DE69632456T2 (de)
DK (1) DK0815114T3 (de)
WO (1) WO1996028457A1 (de)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030232361A1 (en) * 1993-10-26 2003-12-18 Affymetrix, Inc. Nucleic acid array preparation using purified phosphoramidites
WO1998020967A1 (en) * 1996-11-14 1998-05-22 Affymetrix, Inc. Chemical amplification for the synthesis of patterned arrays
US6326489B1 (en) 1997-08-05 2001-12-04 Howard Hughes Medical Institute Surface-bound, bimolecular, double-stranded DNA arrays
US6511803B1 (en) 1997-10-10 2003-01-28 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
CA2305449A1 (en) 1997-10-10 1999-04-22 President & Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6485944B1 (en) 1997-10-10 2002-11-26 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6222030B1 (en) * 1998-08-03 2001-04-24 Agilent Technologies, Inc. Solid phase synthesis of oligonucleotides using carbonate protecting groups and alpha-effect nucleophile deprotection
US6255476B1 (en) 1999-02-22 2001-07-03 Pe Corporation (Ny) Methods and compositions for synthesis of labelled oligonucleotides and analogs on solid-supports
CA2370478A1 (en) 1999-03-24 2000-09-28 Serge L. Beaucage N-acylphosphoramidites and their use in oligonucleotide synthesis
US6824987B1 (en) * 1999-05-11 2004-11-30 President And Fellows Of Harvard College Small molecule printing
US7932213B2 (en) * 1999-05-11 2011-04-26 President And Fellows Of Harvard College Small molecule printing
US6306599B1 (en) 1999-07-16 2001-10-23 Agilent Technologies Inc. Biopolymer arrays and their fabrication
US6451998B1 (en) 1999-10-18 2002-09-17 Agilent Technologies, Inc. Capping and de-capping during oligonucleotide synthesis
US6489466B2 (en) * 2000-01-28 2002-12-03 Linden Technologies, Inc. C-3′ protected monomeric nucleotides and synthesis of oligonucleotides on solid support
EP1176151B1 (de) * 2000-07-28 2014-08-20 Agilent Technologies, Inc. Synthese von Polynukleotiden unter Gebrauch von kombinierter Oxidations- und Entschützungschemie
US20020081589A1 (en) * 2000-10-12 2002-06-27 Jing-Shan Hu Gene expression monitoring using universal arrays
WO2002077194A2 (en) * 2001-03-26 2002-10-03 Linden Technologies, Inc. Polymer synthesis
US20020136772A1 (en) * 2001-03-26 2002-09-26 Tai-Nang Huang Polymer synthesis
US6852850B2 (en) 2001-10-31 2005-02-08 Agilent Technologies, Inc. Use of ionic liquids for fabrication of polynucleotide arrays
US6858720B2 (en) 2001-10-31 2005-02-22 Agilent Technologies, Inc. Method of synthesizing polynucleotides using ionic liquids
WO2003048179A2 (en) * 2001-12-03 2003-06-12 The Government Of The United States Of America, Represented By The Secretary Of The Department Of Health And Human Services Thermolabile hydroxyl protecting groups and methods of use
US20030170637A1 (en) * 2002-03-06 2003-09-11 Pirrung Michael C. Method of analyzing mRNA splice variants
CA2526690C (en) 2003-05-23 2014-01-14 Board Of Regents The University Of Texas System Structure based and combinatorially selected oligonucleoside phosphorothioate and phosphorodithioate aptamer targeting ap-1 transcription factors
US7276599B2 (en) * 2003-06-02 2007-10-02 Isis Pharmaceuticals, Inc. Oligonucleotide synthesis with alternative solvents
US20090317873A1 (en) * 2004-05-04 2009-12-24 Sridhar Govindarajan Design, synthesis and assembly of synthetic nucleic acids
US20060094024A1 (en) * 2004-11-01 2006-05-04 Pirrung Michael C Electrochemical arrays
WO2006065751A2 (en) * 2004-12-13 2006-06-22 Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services Cpg oligonucleotide prodrugs, compositions thereof and associated therapeutic methods
WO2008013569A2 (en) * 2006-01-03 2008-01-31 The President And Fellows Of Harvard College Small molecule printing
US8097711B2 (en) * 2006-09-02 2012-01-17 Agilent Technologies, Inc. Thioether substituted aryl carbonate protecting groups
KR20100025328A (ko) * 2008-08-27 2010-03-09 삼성전자주식회사 이중가닥 영역과 말단 단일가닥 영역을 포함하는 이중가닥 핵산 프로브가 고정된 마이크로어레이를 제조하는 방법
US8158391B2 (en) 2009-05-06 2012-04-17 Dna Twopointo, Inc. Production of an α-carboxyl-ω-hydroxy fatty acid using a genetically modified Candida strain

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4415732A (en) * 1981-03-27 1983-11-15 University Patents, Inc. Phosphoramidite compounds and processes
US5547839A (en) * 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
US5242974A (en) * 1991-11-22 1993-09-07 Affymax Technologies N.V. Polymer reversal on solid surfaces
US5143854A (en) * 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5744101A (en) * 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
WO1992010588A1 (en) * 1990-12-06 1992-06-25 Affymax Technologies N.V. Sequencing by hybridization of a target nucleic acid to a matrix of defined oligonucleotides
ES2155822T3 (es) * 1990-12-06 2001-06-01 Affymetrix Inc Compuestos y su utilizacion en una estrategia de sintesis binaria.
US5486633A (en) * 1994-11-10 1996-01-23 Duke University Photochemically-removable silyl protecting groups

Also Published As

Publication number Publication date
US5908926A (en) 1999-06-01
JPH11501936A (ja) 1999-02-16
AU5422896A (en) 1996-10-02
EP0815114A4 (de) 1999-02-24
EP0815114A1 (de) 1998-01-07
WO1996028457A1 (en) 1996-09-19
DE69632456T2 (de) 2005-07-14
CA2215657A1 (en) 1996-09-19
EP0815114B1 (de) 2004-05-12
DK0815114T3 (da) 2004-08-30
DE69632456D1 (de) 2004-06-17

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