ATE266733T1 - Verfahren zur herstellung von heterologen polypeptiden in transformierten hefezellen - Google Patents

Verfahren zur herstellung von heterologen polypeptiden in transformierten hefezellen

Info

Publication number
ATE266733T1
ATE266733T1 AT99941413T AT99941413T ATE266733T1 AT E266733 T1 ATE266733 T1 AT E266733T1 AT 99941413 T AT99941413 T AT 99941413T AT 99941413 T AT99941413 T AT 99941413T AT E266733 T1 ATE266733 T1 AT E266733T1
Authority
AT
Austria
Prior art keywords
saccharomyces
heterologue
polypeptides
producing
yeast cells
Prior art date
Application number
AT99941413T
Other languages
English (en)
Inventor
Aradhana Srivastava
Jure Piskur
Jens Nielsen
Michi Egel-Mitani
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Application granted granted Critical
Publication of ATE266733T1 publication Critical patent/ATE266733T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/23—Aspartic endopeptidases (3.4.23)
    • C12Y304/23025—Saccharopepsin (3.4.23.25), i.e. yeast proteinase A

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Mycology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Botany (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AT99941413T 1998-09-09 1999-09-08 Verfahren zur herstellung von heterologen polypeptiden in transformierten hefezellen ATE266733T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DKPA199801131 1998-09-09
DKPA199900052 1999-01-18
PCT/DK1999/000473 WO2000014258A1 (en) 1998-09-09 1999-09-08 Method for the production of heterologous polypeptides in transformed yeast cells

Publications (1)

Publication Number Publication Date
ATE266733T1 true ATE266733T1 (de) 2004-05-15

Family

ID=26063197

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99941413T ATE266733T1 (de) 1998-09-09 1999-09-08 Verfahren zur herstellung von heterologen polypeptiden in transformierten hefezellen

Country Status (6)

Country Link
EP (1) EP1109922B1 (de)
JP (1) JP2002524082A (de)
AT (1) ATE266733T1 (de)
AU (1) AU5504099A (de)
DE (1) DE69917294T2 (de)
WO (1) WO2000014258A1 (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR0010806B1 (pt) * 1999-05-21 2014-02-18 Métodos e materiais para a síntese de produtos orgânicos
SE0002460D0 (sv) 2000-06-29 2000-06-29 Roslyn Bill Modified yeast
SE0004241D0 (sv) * 2000-06-29 2000-11-17 Gothia Yeast Solutions Ab Ubv Modified yeast
MXPA04004523A (es) 2001-11-14 2004-08-11 Procter & Gamble Composicion para el lavado automatico de platos y utensilios de cocina en forma de dosis unitaria que contiene un polimero antiescamas.
CA3068459A1 (en) * 2017-06-30 2019-01-03 Ptt Global Chemical Public Company Limited Microorganism with stabilized copy number of functional dna sequence and associated methods

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69632630T2 (de) * 1995-03-16 2005-05-25 Novozymes A/S Enzym mit aminopeptidaseactivität
US6190914B1 (en) * 1996-12-12 2001-02-20 Universiteit Van Amsterdam Methods for modulating metabolic pathways of micro-organisms and micro-organisms obtainable by said methods
EP0954589A1 (de) * 1997-01-24 1999-11-10 Novo Nordisk A/S Sythetische signalpeptid-sequenzen.

Also Published As

Publication number Publication date
EP1109922B1 (de) 2004-05-12
WO2000014258A1 (en) 2000-03-16
DE69917294D1 (de) 2004-06-17
DE69917294T2 (de) 2005-05-12
EP1109922A1 (de) 2001-06-27
AU5504099A (en) 2000-03-27
JP2002524082A (ja) 2002-08-06

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