ATE270334T1 - Verfahren zur verbesserung der transformationseffizienz. - Google Patents

Verfahren zur verbesserung der transformationseffizienz.

Info

Publication number
ATE270334T1
ATE270334T1 AT98901210T AT98901210T ATE270334T1 AT E270334 T1 ATE270334 T1 AT E270334T1 AT 98901210 T AT98901210 T AT 98901210T AT 98901210 T AT98901210 T AT 98901210T AT E270334 T1 ATE270334 T1 AT E270334T1
Authority
AT
Austria
Prior art keywords
transformation efficiency
improving transformation
improving
efficiency
transformation
Prior art date
Application number
AT98901210T
Other languages
English (en)
Inventor
Susan Jayne
Eric Barbour
Terry Meyer
Original Assignee
Pioneer Hi Bred Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred Int filed Critical Pioneer Hi Bred Int
Application granted granted Critical
Publication of ATE270334T1 publication Critical patent/ATE270334T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Steroid Compounds (AREA)
  • Prostheses (AREA)
  • Diaphragms For Electromechanical Transducers (AREA)
AT98901210T 1997-01-14 1998-01-09 Verfahren zur verbesserung der transformationseffizienz. ATE270334T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US3556097P 1997-01-14 1997-01-14
PCT/US1998/000359 WO1998030701A1 (en) 1997-01-14 1998-01-09 Methods for improving transformation efficiency

Publications (1)

Publication Number Publication Date
ATE270334T1 true ATE270334T1 (de) 2004-07-15

Family

ID=21883449

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98901210T ATE270334T1 (de) 1997-01-14 1998-01-09 Verfahren zur verbesserung der transformationseffizienz.

Country Status (10)

Country Link
US (1) US6096947A (de)
EP (1) EP0954584B1 (de)
AR (1) AR011527A1 (de)
AT (1) ATE270334T1 (de)
AU (1) AU729122B2 (de)
CA (1) CA2278028C (de)
DE (1) DE69824832T2 (de)
NZ (1) NZ336687A (de)
WO (1) WO1998030701A1 (de)
ZA (1) ZA98260B (de)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
UY24959A1 (es) * 1997-04-18 1998-10-14 Mogen Int Nv Gen codificador de la cianamida hidratasa como marcador de seleccion para plantas
AU7968098A (en) 1997-06-11 1998-12-30 United States Of America, As Represented By The Secretary Of Agriculture, The Transformation of wheat with the cyanamide hydratase gene
US6586661B1 (en) 1997-06-12 2003-07-01 North Carolina State University Regulation of quinolate phosphoribosyl transferase expression by transformation with a tobacco quinolate phosphoribosyl transferase nucleic acid
IL122270A0 (en) * 1997-11-20 1998-04-05 Yeda Res & Dev DNA molecules conferring to plants resistance to a herbicide and plants transformed thereby
AU1127000A (en) * 1998-10-23 2000-05-15 Mycogen Corporation Plant-optimized marker genes
US6395485B1 (en) * 2000-01-11 2002-05-28 Aventis Cropscience N.V. Methods and kits for identifying elite event GAT-ZM1 in biological samples
MXPA02012733A (es) 2000-06-23 2003-05-14 Du Pont Extrusturas recombinantes y su uso en la reduccion de la expresion de genes.
AU2001286843A1 (en) 2000-08-30 2002-03-13 North Carolina State University Transgenic plants containing molecular decoys that alter protein content therein
DE60128149D1 (en) 2000-11-07 2007-06-06 Univ North Carolina State Putrescin-n-methyltransferasepromotor
CN1638655A (zh) 2001-06-08 2005-07-13 韦克多烟草有限公司 修饰烟草中烟碱和亚硝胺的水平
US20070169227A1 (en) 2003-12-16 2007-07-19 Pioneer Hi-Bred International Inc. Dominant Gene Suppression Transgenes and Methods of Using Same
PT1696721E (pt) 2003-12-16 2010-05-06 Pioneer Hi Bred Int Transgénicos com supressão de gene dominante e processos para a sua utilização
EP2018435B1 (de) 2006-05-17 2012-07-11 Pioneer Hi-Bred International Inc. Künstliche Pflanzenminichromosome
AU2010339481B2 (en) 2009-12-30 2016-02-04 Pioneer Hi-Bred International, Inc. Methods and compositions for targeted polynucleotide modification
WO2012074868A2 (en) 2010-12-03 2012-06-07 Ms Technologies, Llc Optimized expression of glyphosate resistance encoding nucleic acid molecules in plant cells
JP6242345B2 (ja) 2012-02-01 2017-12-06 ダウ アグロサイエンシィズ エルエルシー グリホサート抵抗性植物および関連する方法
US9644213B2 (en) 2012-03-09 2017-05-09 Board Of Trustees Of Michigan State University Method of enhancing plant drought tolerance by expression of NDR1
WO2014107660A2 (en) * 2013-01-04 2014-07-10 Novogy, Inc. Microorganisms engineered to use unconventional sources of nitrogen
WO2018106470A1 (en) * 2016-12-08 2018-06-14 Syngenta Participations Ag Methods for improving transformation frequency
WO2019169150A1 (en) 2018-03-02 2019-09-06 Pioneer Hi-Bred International, Inc. Plant health assay
MX2021011836A (es) * 2019-04-01 2021-10-22 Syngenta Crop Protection Ag Aumento de la transformabilidad de las plantas por transferencia de citotipo.
EP3757219A1 (de) * 2019-06-28 2020-12-30 KWS SAAT SE & Co. KGaA Verbesserte pflanzenregeneration und -umwandlung unter verwendung des grf1-booster-gens
CN114736912B (zh) * 2022-03-24 2023-05-26 华南农业大学 优化的玉米rZmG2基因及其在提高植物遗传转化效率中的应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5380831A (en) * 1986-04-04 1995-01-10 Mycogen Plant Science, Inc. Synthetic insecticidal crystal protein gene
GB9504446D0 (en) * 1995-03-06 1995-04-26 Medical Res Council Improvements in or relating to gene expression

Also Published As

Publication number Publication date
NZ336687A (en) 2001-08-31
EP0954584A1 (de) 1999-11-10
CA2278028A1 (en) 1998-07-16
AU729122B2 (en) 2001-01-25
CA2278028C (en) 2007-11-13
WO1998030701A1 (en) 1998-07-16
US6096947A (en) 2000-08-01
DE69824832T2 (de) 2005-07-07
AU5734198A (en) 1998-08-03
EP0954584B1 (de) 2004-06-30
DE69824832D1 (de) 2004-08-05
AR011527A1 (es) 2000-08-30
ZA98260B (en) 1998-07-13

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