ATE270337T1 - Materialen und methoden im zusammenhang mit bindung und präsentation von substanzen auf zelloberflächen - Google Patents

Materialen und methoden im zusammenhang mit bindung und präsentation von substanzen auf zelloberflächen

Info

Publication number
ATE270337T1
ATE270337T1 AT96929431T AT96929431T ATE270337T1 AT E270337 T1 ATE270337 T1 AT E270337T1 AT 96929431 T AT96929431 T AT 96929431T AT 96929431 T AT96929431 T AT 96929431T AT E270337 T1 ATE270337 T1 AT E270337T1
Authority
AT
Austria
Prior art keywords
presentation
substances
binding
materials
cell surfaces
Prior art date
Application number
AT96929431T
Other languages
English (en)
Inventor
Lothar Steidler
Ledeganckstraat
Erik Remaut
Jeremy Mark Wells
Court Road Tennis
Original Assignee
Lothar Steidler
Erik Remaut
Jeremy Mark Wells
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lothar Steidler, Erik Remaut, Jeremy Mark Wells filed Critical Lothar Steidler
Application granted granted Critical
Publication of ATE270337T1 publication Critical patent/ATE270337T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/08—Clostridium, e.g. Clostridium tetani
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/305—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
    • C07K14/31—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/36—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinomyces; from Streptomyces (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/16—Enzymes or microbial cells immobilised on or in a biological cell
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/746—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for lactic acid bacteria (Streptococcus; Lactococcus; Lactobacillus; Pediococcus; Enterococcus; Leuconostoc; Propionibacterium; Bifidobacterium; Sporolactobacillus)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/035—Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/22—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a Strep-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/55—Fusion polypeptide containing a fusion with a toxin, e.g. diphteria toxin

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Mirrors, Picture Frames, Photograph Stands, And Related Fastening Devices (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Sheet Holders (AREA)
  • Vehicle Interior And Exterior Ornaments, Soundproofing, And Insulation (AREA)
  • Details Of Rigid Or Semi-Rigid Containers (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Adhesive Tapes (AREA)
AT96929431T 1995-09-07 1996-09-06 Materialen und methoden im zusammenhang mit bindung und präsentation von substanzen auf zelloberflächen ATE270337T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9518323.2A GB9518323D0 (en) 1995-09-07 1995-09-07 Materials and methods relating to the attachment and display of substances on cell surfaces
PCT/GB1996/002195 WO1997009437A1 (en) 1995-09-07 1996-09-06 Materials and methods relating to the attachment and display of substances on cell surfaces

Publications (1)

Publication Number Publication Date
ATE270337T1 true ATE270337T1 (de) 2004-07-15

Family

ID=10780370

Family Applications (1)

Application Number Title Priority Date Filing Date
AT96929431T ATE270337T1 (de) 1995-09-07 1996-09-06 Materialen und methoden im zusammenhang mit bindung und präsentation von substanzen auf zelloberflächen

Country Status (16)

Country Link
US (1) US6190662B1 (de)
EP (1) EP0848756B1 (de)
JP (1) JP4180111B2 (de)
KR (1) KR19990044528A (de)
CN (1) CN1201493A (de)
AT (1) ATE270337T1 (de)
AU (1) AU729449B2 (de)
BR (1) BR9610133A (de)
CA (1) CA2231372A1 (de)
DE (1) DE69632827T2 (de)
GB (1) GB9518323D0 (de)
MX (1) MX9801864A (de)
NO (1) NO980976L (de)
NZ (1) NZ316580A (de)
WO (1) WO1997009437A1 (de)
ZA (1) ZA967562B (de)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5821088A (en) * 1990-05-11 1998-10-13 Siga Pharmaceuticals, Inc. Use of gram-positive bacteria to express recombinant proteins
GB9521568D0 (en) * 1995-10-20 1995-12-20 Lynxvale Ltd Delivery of biologically active polypeptides
DE19735105A1 (de) * 1997-08-13 1999-03-04 Univ Albert Ludwigs Freiburg Transportsystem zur Einbringung von Proteinen in Zielzellen mit Hilfe eines Fusionsproteins, Nucleinsäurekonstrukte kodierend für die Komponenten des Transportsystems und Arzneimittel, die Komponenten des Transportsystems umfassen
EP0916726A1 (de) * 1997-11-13 1999-05-19 Rijksuniversiteit te Groningen Befestigungs-Substanzen an Mikroorganismen
US6100388A (en) * 1998-03-16 2000-08-08 Biogaia Biologies Ab Lactobacilli harboring aggregation gene as a vaccine delivery vehicle
EP1012245A1 (de) * 1998-06-25 2000-06-28 Korea Green Cross Corporation Oberflächenexpressionssystem von hepatitis b virus oberflächenantigen und kernantigen von hepatitis c virus unter verwendung von eis-kern protein
KR100313135B1 (ko) 1999-02-22 2001-11-05 윤덕용 세포 표면 발현 모체로서의 대장균 유래 세포외막 단백질 ｃ(ｏ
PT1739178E (pt) 1999-07-05 2010-11-17 Actogenix Nv Libertação de péptidos trifólios
IL154284A0 (en) * 2000-09-25 2003-09-17 Nestle Sa Lactic acid bacteria capable of reducing an individual's tendency to develop allergic reactions
CN1484700A (zh) * 2000-11-02 2004-03-24 �¼��¹�����ѧ aopB基因,蛋白,同系物,片段和它们的变体,以及它们在细胞表面展示方面的应用
EP1239032A1 (de) 2001-03-02 2002-09-11 Société des Produits Nestlé S.A. Milchsäurebakterien als Mitteln zur Behandlung und Vorbeugung von Allergie
US7780961B2 (en) * 2001-05-03 2010-08-24 Actogenix N.V. Self-containing Lactococcus strain
JP2005529622A (ja) * 2002-06-19 2005-10-06 ヴェーイーベー・ヴェーゼットウェー 腸吸収を促進するための方法および手段
US7527966B2 (en) * 2002-06-26 2009-05-05 Transgenrx, Inc. Gene regulation in transgenic animals using a transposon-based vector
WO2004041863A2 (en) 2002-11-08 2004-05-21 Ablynx N.V. Single domain antibodies directed against interferon- gamma and uses therefor
EP1558645B1 (de) 2002-11-08 2011-07-27 Ablynx N.V. Stabilisierte einzel-domänen-antikörper in einer pharmazeutischen zusammensetzung ausgestaltet für inhalation
EP1900753B1 (de) 2002-11-08 2017-08-09 Ablynx N.V. Verfahren zur Verabreichung therapeutischer Polypeptide und Polypeptide dafür
AU2003298291A1 (en) * 2002-11-15 2004-06-15 Universiteit Gent Self-containing lactobacillus strain
EP1590443A4 (de) * 2003-01-29 2009-01-07 Osel Inc Oberflächenexpression biologisch aktiver proteine in bakterien
CA2539452C (en) * 2003-09-17 2013-10-29 Guava Technologies, Inc. Compositions and methods for analysis of target analytes
WO2006083516A2 (en) * 2005-01-12 2006-08-10 Osel, Inc. Modified cyanovirin-n polypeptide
ES2405552T3 (es) 2005-11-29 2013-05-31 Actogenix N.V. Inducción de tolerancia mucosa a antiantígenos de células beta de islotes pancreáticos
WO2008090223A2 (en) 2007-01-25 2008-07-31 Actogenix N.V. Treatment of immune disease by mucosal delivery of antigens using genetically modified lactobacillus
EP1972350A1 (de) * 2007-03-20 2008-09-24 Rijksuniversiteit Groningen Duales Targeting-System
WO2009137763A2 (en) * 2008-05-08 2009-11-12 Emory University Methods and compositions for the display of polypeptides on the pili of gram-positive bacteria
EP3181682B1 (de) * 2008-09-29 2021-04-21 Intrexon Actobiotics NV Reduzierte mikrobenkolonisierung an der schleimhaut
EP2892554B1 (de) 2012-09-07 2020-08-26 Emory University Hiv-immunstimulierende zusammensetzungen mit rekombinant exprimierten pili auf bakterien und entsprechende verfahren
EP2897638A1 (de) 2012-09-24 2015-07-29 Montana State University-Bozeman Rekombinanter lactococcus lactis zur expression des escherichia coli-kolonisationsfaktor-antigens i (cfa/i) fimbriae und verfahren zu deren verwendung
WO2018208726A1 (en) 2017-05-08 2018-11-15 Rensselaer Polytechnic Institute Multiplex detection of bacterial pathogens via cell wall binding domain complexes
AR118443A1 (es) * 2019-03-19 2021-10-06 Bayer Cropscience Lp Proteínas de fusión, bacterias recombinantes y fragmentos de exosporas para control de plagas y salud de las plantas
AU2021410776A1 (en) * 2020-12-23 2023-05-04 Chan Zuckerberg Biohub, Inc. Bacteria-engineered to elicit antigen-specific t cells

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5616686A (en) 1990-05-11 1997-04-01 The Rockefeller University Polypeptide of a hybrid surface protein by bacteria
US5348867A (en) * 1991-11-15 1994-09-20 George Georgiou Expression of proteins on bacterial surface
AU674311B2 (en) * 1992-03-13 1996-12-19 Rockefeller University, The Delivery and expression of a hybrid surface protein on the surface of gram positive bacteria

Also Published As

Publication number Publication date
KR19990044528A (ko) 1999-06-25
AU729449B2 (en) 2001-02-01
US6190662B1 (en) 2001-02-20
JP4180111B2 (ja) 2008-11-12
BR9610133A (pt) 1999-12-21
NO980976D0 (no) 1998-03-06
DE69632827T2 (de) 2005-07-07
EP0848756A1 (de) 1998-06-24
WO1997009437A1 (en) 1997-03-13
CA2231372A1 (en) 1997-03-13
GB9518323D0 (en) 1995-11-08
NO980976L (no) 1998-05-06
MX9801864A (es) 1998-11-29
AU6884496A (en) 1997-03-27
HK1014337A1 (en) 1999-09-24
JPH11511983A (ja) 1999-10-19
NZ316580A (en) 1999-09-29
DE69632827D1 (de) 2004-08-05
ZA967562B (en) 1998-03-06
CN1201493A (zh) 1998-12-09
EP0848756B1 (de) 2004-06-30

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