ATE271605T1 - Ph abhängige iontauschermatrix und anwendungsverfahren in der isolierung von nukleinsäuren - Google Patents

Ph abhängige iontauschermatrix und anwendungsverfahren in der isolierung von nukleinsäuren

Info

Publication number
ATE271605T1
ATE271605T1 AT00935865T AT00935865T ATE271605T1 AT E271605 T1 ATE271605 T1 AT E271605T1 AT 00935865 T AT00935865 T AT 00935865T AT 00935865 T AT00935865 T AT 00935865T AT E271605 T1 ATE271605 T1 AT E271605T1
Authority
AT
Austria
Prior art keywords
ion exchange
isolation
nucleic acids
application methods
exchange matrix
Prior art date
Application number
AT00935865T
Other languages
English (en)
Inventor
Craig E Smith
Diana L Holmes
Daniel J Simpson
Jehoshua Katzhendler
Rex M Bitner
Josephine C Grosch
Original Assignee
Promega Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Promega Corp filed Critical Promega Corp
Application granted granted Critical
Publication of ATE271605T1 publication Critical patent/ATE271605T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J39/00—Cation exchange; Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
    • B01J39/26—Cation exchangers for chromatographic processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
    • B01J41/20—Anion exchangers for chromatographic processes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AT00935865T 1999-05-14 2000-05-05 Ph abhängige iontauschermatrix und anwendungsverfahren in der isolierung von nukleinsäuren ATE271605T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/312,172 US6310199B1 (en) 1999-05-14 1999-05-14 pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
PCT/US2000/012186 WO2000069872A2 (en) 1999-05-14 2000-05-05 pH DEPENDENT ION EXCHANGE MATRIX AND METHOD OF USE IN THE ISOLATION OF NUCLEIC ACIDS

Publications (1)

Publication Number Publication Date
ATE271605T1 true ATE271605T1 (de) 2004-08-15

Family

ID=23210195

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00935865T ATE271605T1 (de) 1999-05-14 2000-05-05 Ph abhängige iontauschermatrix und anwendungsverfahren in der isolierung von nukleinsäuren

Country Status (8)

Country Link
US (2) US6310199B1 (de)
EP (1) EP1179057B1 (de)
JP (1) JP4377550B2 (de)
AT (1) ATE271605T1 (de)
AU (1) AU772046B2 (de)
CA (1) CA2372054A1 (de)
DE (1) DE60012318T2 (de)
WO (1) WO2000069872A2 (de)

Families Citing this family (127)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
KR20010032806A (ko) * 1997-12-06 2001-04-25 매튜 존 베이커 핵산 분리방법
US6914137B2 (en) * 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
US7078224B1 (en) * 1999-05-14 2006-07-18 Promega Corporation Cell concentration and lysate clearance using paramagnetic particles
US6310199B1 (en) * 1999-05-14 2001-10-30 Promega Corporation pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
AU2594202A (en) * 2000-11-13 2002-05-21 Promega Corp Lysate clearance and nucleic acid isolation using silanized silica matrices
SE0004932D0 (sv) 2000-12-31 2000-12-31 Apbiotech Ab A method for mixed mode adsorption and mixed mode adsorbents
AU2002235795A1 (en) * 2000-12-31 2002-07-16 Amersham Biosciences Ab A method for the manufacture of compositions containing low concentrations of salts
US7829025B2 (en) 2001-03-28 2010-11-09 Venture Lending & Leasing Iv, Inc. Systems and methods for thermal actuation of microfluidic devices
US8895311B1 (en) 2001-03-28 2014-11-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
AU2003202026A1 (en) * 2002-01-16 2003-09-02 Dynal Biotech Asa Method for isolating nucleic acids and protein from a single sample
US20030215845A1 (en) * 2002-02-19 2003-11-20 Bille Todd William Selective extraction of DNA from groups of cells
AU2003216830A1 (en) * 2002-03-21 2003-10-08 Avecia Biotechnology Inc. Purification methods for oligonucleotides and their analogs
US20060035224A1 (en) * 2002-03-21 2006-02-16 Johansen Jack T Purification methods for oligonucleotides and their analogs
US7052840B2 (en) * 2002-04-03 2006-05-30 Capitol Genomix, Inc. Reversible association of nucleic acid with a carboxylated substrate
GB0210766D0 (en) * 2002-05-10 2002-06-19 Genovision As Isolating nucleic acid
US7597936B2 (en) * 2002-11-26 2009-10-06 University Of Utah Research Foundation Method of producing a pigmented composite microporous material
JP4824312B2 (ja) * 2002-11-26 2011-11-30 ユニバーシティ オブ ユタ リサーチ ファンデーション 微小孔物質、被分析物の局在化及び定量の方法、並びに被分析物の局在化及び定量用物品
GB0229287D0 (en) * 2002-12-16 2003-01-22 Dna Res Innovations Ltd Polyfunctional reagents
US20050042660A1 (en) * 2003-07-31 2005-02-24 Hall Gerald Edward Devices and methods for isolating RNA
US20050026153A1 (en) * 2003-07-31 2005-02-03 Iannotti Claudia A. Devices and methods for isolating RNA
JP4996248B2 (ja) 2003-07-31 2012-08-08 ハンディーラブ インコーポレイテッド 粒子含有サンプルの処理
DE10340746A1 (de) * 2003-09-01 2005-03-31 Decoma (Germany) Gmbh Dachboxeinrichtung, und Dachmodul mit integrierter Dachboxeinrichtung
EP1529840A1 (de) * 2003-11-04 2005-05-11 Qiagen GmbH Ein schnelles und preiswertes Verfahren zur Gewinnung von Nukleinsäuren
DE10355409A1 (de) * 2003-11-25 2005-06-30 Magnamedics Gmbh Sphärische, magnetische Silicagel-Träger mit vergrößerter Oberfläche für die Aufreinigung von Nukleinsäuren
US20060160122A1 (en) * 2004-02-18 2006-07-20 Applera Corporation Polyelectrolyte-coated size-exclusion ion-exchange particles
US20050181378A1 (en) * 2004-02-18 2005-08-18 Applera Corporation Polyelectrolyte-coated size-exclusion ion-exchange particles
SE0400490D0 (sv) * 2004-02-26 2004-02-26 Amersham Biosciences Ab Plasmid purification
EP2345739B8 (de) * 2004-05-03 2016-12-07 Handylab, Inc. Mikrofluidische Vorrichtung zur Verarbeitung polynukleotidhaltiger Proben
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
DE102004023586A1 (de) * 2004-05-13 2005-12-08 Universität Bremen Protonenleitfähiges, vernetztes Heteropolysiloxan, protonenleitfähige Membran und Verfahren zu deren Herstellung
US20060099605A1 (en) * 2004-11-11 2006-05-11 Hall Gerald E Jr Devices and methods for isolating RNA
US7402243B2 (en) * 2004-09-10 2008-07-22 Dionex Corporation Organosilanes and substrate bonded with same
EP1693108A1 (de) * 2004-12-04 2006-08-23 MERCK PATENT GmbH Mischmodus Anionenaustauschertrennungsmaterial
KR100647306B1 (ko) * 2004-12-23 2006-11-23 삼성전자주식회사 아미노기와 카르복실기를 포함하고 제1 ｐＨ에서 양전하를띠는 물질을 이용하여 핵산을 분리하는 방법
US7964380B2 (en) * 2005-01-21 2011-06-21 Argylia Technologies Nanoparticles for manipulation of biopolymers and methods of thereof
KR100647315B1 (ko) * 2005-02-02 2006-11-23 삼성전자주식회사 실란화된 고상 물질을 이용한 핵산의 분리 및 증폭 방법
US7468130B2 (en) * 2005-02-15 2008-12-23 Dionex Corporation Organosilanes and substrates covalently bonded with same and methods for synthesis and use same
US20060223072A1 (en) * 2005-03-31 2006-10-05 Boyes Barry E Methods of using a DNase I-like enzyme
US20060223073A1 (en) * 2005-03-31 2006-10-05 Boyes Barry E Methods of using a DNase I-like enzyme
KR100657957B1 (ko) * 2005-04-12 2006-12-14 삼성전자주식회사 아미노기와 카르복실기를 포함하고 제1 ｐＨ에서 양전하를띠는 물질을 이용하여 핵산을 분리하는 방법 및 상기방법에 사용될 수 있는 핵산 분리용 고상 물질
US20060234251A1 (en) * 2005-04-19 2006-10-19 Lumigen, Inc. Methods of enhancing isolation of RNA from biological samples
ES2381279T3 (es) 2005-05-06 2012-05-24 Gen-Probe Incorporated Métodos y productos para la captura de ácido nucleico diana
KR100668337B1 (ko) * 2005-05-21 2007-01-12 삼성전자주식회사 단백질에 비하여 핵산에 대한 결합력이 선택적으로 높은ｐＨ 의존성 이온 교환물질, 그가 고정화되어 있는 고체기판, 및 상기 물질 및 고체 기판을 이용하여 핵산을분리하는 방법
KR100668338B1 (ko) 2005-05-21 2007-01-12 삼성전자주식회사 신규한 ｐＨ 의존성 이온 교환물질, 그가 고정화되어 있는고체 기판, 및 상기 물질 및 고체 기판을 이용하여 핵산을분리하는 방법
US20060270843A1 (en) * 2005-05-26 2006-11-30 Hall Gerald E Jr Methods for isolation of nucleic acids
US20070015191A1 (en) * 2005-07-01 2007-01-18 Promega Corporation Network of buoyant particles for biomolecule purification and use of buoyant particles or network of buoyant particles for biomolecule purification
CN101258175B (zh) 2005-08-03 2011-11-16 默克专利股份公司 亲水交联聚合物
EP1754534A1 (de) 2005-08-03 2007-02-21 MERCK PATENT GmbH Hydrophiles vernetztes Polymer
EP1963526A4 (de) 2005-12-09 2009-11-18 Promega Corp Nukleinsäurereinigung mittels bindungsmatrix
KR100829574B1 (ko) * 2006-01-03 2008-05-14 삼성전자주식회사 마이크로어레이용 기판, 그 마이크로어레이용 기판을이용하여 생분자를 분석하는 방법, 및 그 마이크로어레이용기판을 포함하는 랩온어칩
US20070190526A1 (en) * 2006-02-16 2007-08-16 Nexgen Diagnostics Llc Methods of extracting nucleic acids
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
WO2007112114A2 (en) 2006-03-24 2007-10-04 Handylab, Inc. Integrated system for processing microfluidic samples, and method of using same
US8883490B2 (en) 2006-03-24 2014-11-11 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
JP4331256B2 (ja) * 2006-04-12 2009-09-16 パナソニック株式会社 有機分子膜構造体の形成方法
US20080102493A1 (en) * 2006-06-29 2008-05-01 Millipore Corporation Isolation of RNA and DNA from a biological sample
US20080026375A1 (en) * 2006-07-31 2008-01-31 Sigma Aldrich Co. Compositions and Methods for Isolation of Biological Molecules
US20080023395A1 (en) * 2006-07-31 2008-01-31 Sigma Aldrich Co. Compositions and Methods for Isolation of Biological Molecules
US20080026374A1 (en) * 2006-07-31 2008-01-31 Sigma Aldrich Co. Compositions and Methods for Isolation of Biological Molecules
GB2445442A (en) * 2006-09-26 2008-07-09 Ge Healthcare Bio Sciences Nucleic acid purification using anion exchange
GB2445441B (en) * 2006-09-26 2010-06-30 Ge Healthcare Bio Sciences Nucleic acid purification method
GB2443505B (en) * 2006-09-26 2008-12-31 Ge Healthcare Bio Sciences Nucleic acid purification method
US7492312B2 (en) * 2006-11-14 2009-02-17 Fam Adly T Multiplicative mismatched filters for optimum range sidelobe suppression in barker code reception
US8709787B2 (en) 2006-11-14 2014-04-29 Handylab, Inc. Microfluidic cartridge and method of using same
CA2629589C (en) 2007-04-20 2016-03-29 F.Hoffmann-La Roche Ag Isolation and purification of nucleic acid molecules with a solid phase
US7557232B2 (en) * 2007-05-25 2009-07-07 Dionex Corporation Compositions useful as chromatography stationary phases
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
WO2009012185A1 (en) 2007-07-13 2009-01-22 Handylab, Inc. Polynucleotide capture materials, and methods of using same
US9618139B2 (en) 2007-07-13 2017-04-11 Handylab, Inc. Integrated heater and magnetic separator
US8105783B2 (en) 2007-07-13 2012-01-31 Handylab, Inc. Microfluidic cartridge
US8287820B2 (en) 2007-07-13 2012-10-16 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US8569221B2 (en) 2007-08-30 2013-10-29 Kimberly-Clark Worldwide, Inc. Stain-discharging and removing system
WO2009100172A1 (en) 2008-02-07 2009-08-13 Ge Healthcare Bio-Sciences Corp. Isolation of dna, rna and protein from a single sample
JP5999899B2 (ja) 2008-04-08 2016-09-28 バイオ−ラッド ラボラトリーズ インコーポレーティッド 抗体のクロマトグラフィー精製法
GB0814570D0 (en) * 2008-08-08 2008-09-17 Diagnostics For The Real World Isolation of nucleic acid
KR101015502B1 (ko) * 2008-09-09 2011-02-22 삼성전자주식회사 제1 ｐＨ에서 양전하 및 제2 ｐＨ에서 음전하를 띠는 화합물 및 그를 이용하여 핵산을 분리하는 방법
KR20100070994A (ko) 2008-12-18 2010-06-28 토소가부시키가이샤 액체 크로마토그래피용 충전 재료 및 해당 충전 재료에 의한 생체고분자의 분리·정제 방법
DE102008063003A1 (de) * 2008-12-23 2010-06-24 Qiagen Gmbh Nukleinsäureaufreinigungsverfahren
DE102008063001A1 (de) 2008-12-23 2010-06-24 Qiagen Gmbh Nukleinsäureaufreinigungsverfahren
EP2256195A1 (de) 2009-05-12 2010-12-01 Qiagen GmbH Nukleinsäureaufreinigungsverfahren
US8222397B2 (en) 2009-08-28 2012-07-17 Promega Corporation Methods of optimal purification of nucleic acids and kit for use in performing such methods
US8039613B2 (en) 2009-08-28 2011-10-18 Promega Corporation Methods of purifying a nucleic acid and formulation and kit for use in performing such methods
CN102574101A (zh) * 2009-10-12 2012-07-11 通用电气健康护理生物科学股份公司 分离基质
CN102753705A (zh) * 2009-12-14 2012-10-24 B·吴 分离核酸物质的方法和材料
EA201200898A1 (ru) 2009-12-17 2013-05-30 Инстрактион Гмбх Специфичный сорбент для связывания белков и пептидов и способ разделения с его использованием
US20130261003A1 (en) 2010-08-06 2013-10-03 Ariosa Diagnostics, In. Ligation-based detection of genetic variants
US20120034603A1 (en) 2010-08-06 2012-02-09 Tandem Diagnostics, Inc. Ligation-based detection of genetic variants
US11203786B2 (en) 2010-08-06 2021-12-21 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US11031095B2 (en) 2010-08-06 2021-06-08 Ariosa Diagnostics, Inc. Assay systems for determination of fetal copy number variation
US20140342940A1 (en) 2011-01-25 2014-11-20 Ariosa Diagnostics, Inc. Detection of Target Nucleic Acids using Hybridization
US20130040375A1 (en) 2011-08-08 2013-02-14 Tandem Diagnotics, Inc. Assay systems for genetic analysis
US8700338B2 (en) 2011-01-25 2014-04-15 Ariosa Diagnosis, Inc. Risk calculation for evaluation of fetal aneuploidy
US10533223B2 (en) 2010-08-06 2020-01-14 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US10167508B2 (en) 2010-08-06 2019-01-01 Ariosa Diagnostics, Inc. Detection of genetic abnormalities
US9994897B2 (en) 2013-03-08 2018-06-12 Ariosa Diagnostics, Inc. Non-invasive fetal sex determination
US10131947B2 (en) 2011-01-25 2018-11-20 Ariosa Diagnostics, Inc. Noninvasive detection of fetal aneuploidy in egg donor pregnancies
US11270781B2 (en) 2011-01-25 2022-03-08 Ariosa Diagnostics, Inc. Statistical analysis for non-invasive sex chromosome aneuploidy determination
US8756020B2 (en) 2011-01-25 2014-06-17 Ariosa Diagnostics, Inc. Enhanced risk probabilities using biomolecule estimations
AU2012242510B2 (en) 2011-04-15 2015-05-14 Becton, Dickinson And Company Scanning real-time microfluidic thermocycler and methods for synchronized thermocycling and scanning optical detection
CN102250876B (zh) 2011-05-18 2014-08-13 李学敬 从生物材料中分离纯化rna的方法
WO2013012545A1 (en) 2011-07-20 2013-01-24 Conocophillips Company Surfactant system to increase hydrocarbon recovery
US8712697B2 (en) 2011-09-07 2014-04-29 Ariosa Diagnostics, Inc. Determination of copy number variations using binomial probability calculations
JP5899731B2 (ja) 2011-09-13 2016-04-06 ソニー株式会社 核酸精製方法、核酸抽出方法、及び核酸精製用キット
JP6117217B2 (ja) 2011-09-30 2017-04-19 ベクトン・ディキンソン・アンド・カンパニーＢｅｃｔｏｎ， Ｄｉｃｋｉｎｓｏｎ Ａｎｄ Ｃｏｍｐａｎｙ ユニット化された試薬ストリップ
EP2773892B1 (de) 2011-11-04 2020-10-07 Handylab, Inc. Vorrichtung zur vorbereitung von polynukleotidproben
ES2978107T3 (es) 2012-02-03 2024-09-05 Becton Dickinson Co Archivos externos para distribución de pruebas de diagnóstico molecular y determinación de compatibilidad entre pruebas
US10289800B2 (en) 2012-05-21 2019-05-14 Ariosa Diagnostics, Inc. Processes for calculating phased fetal genomic sequences
CN104583421A (zh) 2012-07-19 2015-04-29 阿瑞奥萨诊断公司 遗传变体的基于多重的顺序连接的检测
IN2015DN02055A (de) 2012-09-17 2015-08-14 Grace W R & Co
ES2929099T3 (es) 2014-05-02 2022-11-24 Grace W R & Co Material de soporte funcionalizado y métodos de fabricación y uso de material de soporte funcionalizado
US10000750B2 (en) 2014-10-09 2018-06-19 Promega Corporation Method of isolating nucleic acid
WO2016096590A1 (en) * 2014-12-16 2016-06-23 Unilever Plc Synthetic enzyme mimic based on a histidine scaffold
ES2896897T3 (es) 2015-06-05 2022-02-28 Grace W R & Co Agentes de clarificación para el bioprocesamiento de adsorbentes y métodos para producir y usar los mismos
FR3038616B1 (fr) * 2015-07-06 2020-11-06 Gl Biocontrol Procede de purification et de concentration d'acides nucleiques.
US20170298413A1 (en) * 2016-04-13 2017-10-19 New York Genome Center Methods for the isolation of biomolecules and uses thereof
WO2017184436A1 (en) * 2016-04-17 2017-10-26 Children's Medical Center Corporation Systems and methods for steroidal gels
CN108097336B (zh) * 2017-10-25 2019-02-26 重庆博蓝鹰生物技术有限公司 两性解离离子交换介质、应用方法和分离容量标定方法
US11896969B2 (en) * 2019-09-05 2024-02-13 Bio-Rad Laboratories, Inc. Anionic exchange-hydrophobic mixed mode chromatography resins
US20230028205A1 (en) * 2019-12-16 2023-01-26 Qiagen Gmbh Enrichment method
US20230183674A1 (en) * 2020-05-25 2023-06-15 Sekisui Medical Co., Ltd. Method for purifying nucleic acid
JP2023530938A (ja) * 2020-06-17 2023-07-20 インテグリス・インコーポレーテッド イオン交換膜、フィルタ及び方法
KR102412078B1 (ko) * 2021-12-23 2022-06-22 홍승범 다공성 이온 전하 결정체를 이용한 핵산 추출방법
WO2024233291A2 (en) * 2023-05-05 2024-11-14 Bio-Rad Laboratories, Inc. Multimodal chromatography resins with anionic and cationic ligands

Family Cites Families (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4297337A (en) 1979-04-13 1981-10-27 Corning Glass Works Solid-phase immunoassays using magnetic glass
US4395271A (en) 1979-04-13 1983-07-26 Corning Glass Works Method for making porous magnetic glass and crystal-containing structures
US4233169A (en) 1979-04-13 1980-11-11 Corning Glass Works Porous magnetic glass structure
US4298500A (en) 1980-05-05 1981-11-03 Varian Associates, Inc. Mixed phase chromatographic compositions
DE3211309A1 (de) 1982-03-26 1983-09-29 Metin Dipl.-Ing. 6100 Darmstadt Colpan Chromatographisches verfahren zur isolierung von makromolekuelen
US4695393A (en) 1983-05-12 1987-09-22 Advanced Magnetics Inc. Magnetic particles for use in separations
US4672040A (en) 1983-05-12 1987-06-09 Advanced Magnetics, Inc. Magnetic particles for use in separations
US4523996A (en) 1984-02-22 1985-06-18 Westinghouse Electric Corp. Method of separating cationic from anionic beads in mixed resin beds
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5076950A (en) 1985-12-20 1991-12-31 Syntex (U.S.A.) Inc. Magnetic composition for particle separation
DE3639949A1 (de) 1986-11-22 1988-06-09 Diagen Inst Molekularbio Verfahren zur trennung von langkettigen nukleinsaeuren
US4767670A (en) 1987-01-21 1988-08-30 E. I. Du Pont De Nemours And Company Chromatographic supports for separation of oligonucleotides
US5075430A (en) 1988-12-12 1991-12-24 Bio-Rad Laboratories, Inc. Process for the purification of DNA on diatomaceous earth
US5234809A (en) 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
US5523231A (en) 1990-02-13 1996-06-04 Amersham International Plc Method to isolate macromolecules using magnetically attractable beads which do not specifically bind the macromolecules
WO1992011933A1 (en) 1991-01-04 1992-07-23 Perseptive Biosystems, Inc. Sulfonamide bonded hydrophilic coatings
US5155018A (en) 1991-07-10 1992-10-13 Hahnemann University Process and kit for isolating and purifying RNA from biological sources
US5395498A (en) 1991-11-06 1995-03-07 Gombinsky; Moshe Method for separating biological macromolecules and means therfor
US5610274A (en) 1991-11-20 1997-03-11 Cpg, Inc. Production and use of magnetic porous inorganic materials
US5734020A (en) 1991-11-20 1998-03-31 Cpg, Inc. Production and use of magnetic porous inorganic materials
US5346994A (en) 1992-01-28 1994-09-13 Piotr Chomczynski Shelf-stable product and process for isolating RNA, DNA and proteins
US5316680A (en) 1992-10-21 1994-05-31 Cornell Research Foundation, Inc. Multimodal chromatographic separation media and process for using same
AT398973B (de) 1992-11-18 1995-02-27 Bonn Guenther Dr Verfahren zur trennung von nukleinsäuren
AU6230594A (en) 1993-02-01 1994-08-29 University Of Iowa Research Foundation, The Quartenary amine surfactants and methods of using same in isolation of rna
DE4307262A1 (de) 1993-03-02 1994-09-08 Christian Bergemann Magnetisches polymeres Siliciumdioxid
JP3696238B2 (ja) 1993-08-30 2005-09-14 プロメガ・コーポレイシヨン 核酸精製用組成物及び方法
US5990301A (en) 1994-02-07 1999-11-23 Qiagen Gmbh Process for the separation and purification of nucleic acids from biological sources
DE59507433D1 (de) 1994-02-07 2000-01-20 Qiagen Gmbh Verfahren zur herstellung endotoxinfreier oder an endotoxin abgereicherter nucleinsäuren und/oder oligonucleotide für die gentherapie
US5582988A (en) 1994-09-15 1996-12-10 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
US5705628A (en) 1994-09-20 1998-01-06 Whitehead Institute For Biomedical Research DNA purification and isolation using magnetic particles
US5652348A (en) 1994-09-23 1997-07-29 Massey University Chromatographic resins and methods for using same
US5654141A (en) 1994-11-18 1997-08-05 Thomas Jefferson University Amplification based detection of bacterial infection
US5660984A (en) 1994-12-09 1997-08-26 Davis; Thomas E. DNA isolating apparatus comprising a non-porous DNA binding, anion exchange resin and methods of use thereof
AR003122A1 (es) 1995-05-19 1998-07-08 Merck & Co Inc Un proceso para aislacion y purificacion de plasmidos en fermentadores de gran escala y el adn aislado y purificado obtenido mediante dicho proceso.
DE19520398B4 (de) 1995-06-08 2009-04-16 Roche Diagnostics Gmbh Magnetisches Pigment
JP2965131B2 (ja) 1995-07-07 1999-10-18 東洋紡績株式会社 核酸結合用磁性担体およびそれを用いる核酸単離方法
US5783686A (en) 1995-09-15 1998-07-21 Beckman Instruments, Inc. Method for purifying nucleic acids from heterogenous mixtures
SE9600590D0 (sv) 1996-02-19 1996-02-19 Pharmacia Biotech Ab Sätt för kromatografisk separation av peptider och nukleinsyra samt ny högaffin jonbytesmatris
JPH09327291A (ja) 1996-06-11 1997-12-22 Toyobo Co Ltd Ｒｎａの抽出精製方法
JP3940935B2 (ja) 1996-06-11 2007-07-04 東洋紡績株式会社 プラスミドｄｎａの抽出精製方法
US6027945A (en) 1997-01-21 2000-02-22 Promega Corporation Methods of isolating biological target materials using silica magnetic particles
HU225261B1 (en) 1997-01-21 2006-08-28 Grace W R & Co Silica adsorbent on magnetic substrate
US6310199B1 (en) * 1999-05-14 2001-10-30 Promega Corporation pH dependent ion exchange matrix and method of use in the isolation of nucleic acids

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US6806362B2 (en) 2004-10-19
CA2372054A1 (en) 2000-11-23
AU772046B2 (en) 2004-04-08
AU5126100A (en) 2000-12-05
JP2002543979A (ja) 2002-12-24
WO2000069872A2 (en) 2000-11-23
EP1179057A2 (de) 2002-02-13
DE60012318T2 (de) 2005-08-04
DE60012318D1 (de) 2004-08-26
JP4377550B2 (ja) 2009-12-02
US20010014650A1 (en) 2001-08-16
US6310199B1 (en) 2001-10-30
WO2000069872A3 (en) 2001-02-15

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