ATE27463T1 - Verfahren zur in vitro transformation von pflanzenprotoplasten mit plasmid-dns. - Google Patents

Verfahren zur in vitro transformation von pflanzenprotoplasten mit plasmid-dns.

Info

Publication number
ATE27463T1
ATE27463T1 AT83200207T AT83200207T ATE27463T1 AT E27463 T1 ATE27463 T1 AT E27463T1 AT 83200207 T AT83200207 T AT 83200207T AT 83200207 T AT83200207 T AT 83200207T AT E27463 T1 ATE27463 T1 AT E27463T1
Authority
AT
Austria
Prior art keywords
plasmid dna
procedure
incubation
plant protoplasts
vitro transformation
Prior art date
Application number
AT83200207T
Other languages
English (en)
Inventor
Robbert Adriaan Schilperoort
Franciscus Andries Krens
George Joseph Wullems
Original Assignee
Univ Leiden
Robbert Adriaan Schilperoort
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Leiden, Robbert Adriaan Schilperoort filed Critical Univ Leiden
Application granted granted Critical
Publication of ATE27463T1 publication Critical patent/ATE27463T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT83200207T 1982-02-11 1983-02-10 Verfahren zur in vitro transformation von pflanzenprotoplasten mit plasmid-dns. ATE27463T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
NL8200523A NL8200523A (nl) 1982-02-11 1982-02-11 Werkwijze voor het in vitro transformeren van planteprotoplasten met plasmide-dna.
EP83200207A EP0086537B1 (de) 1982-02-11 1983-02-10 Verfahren zur in vitro Transformation von Pflanzenprotoplasten mit Plasmid-DNS

Publications (1)

Publication Number Publication Date
ATE27463T1 true ATE27463T1 (de) 1987-06-15

Family

ID=19839239

Family Applications (1)

Application Number Title Priority Date Filing Date
AT83200207T ATE27463T1 (de) 1982-02-11 1983-02-10 Verfahren zur in vitro transformation von pflanzenprotoplasten mit plasmid-dns.

Country Status (6)

Country Link
US (1) US4684611A (de)
EP (1) EP0086537B1 (de)
JP (1) JPH0630589B2 (de)
AT (1) ATE27463T1 (de)
DE (1) DE3371792D1 (de)
NL (1) NL8200523A (de)

Families Citing this family (247)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8334139B1 (en) 1983-01-17 2012-12-18 Monsanto Technology Llc Plasmids for transforming plant cells
WO1984002920A1 (en) 1983-01-17 1984-08-02 Monsanto Co Genetically transformed plants
ATE73845T1 (de) * 1984-05-11 1992-04-15 Ciba Geigy Ag Transformation von pflanzenerbgut.
US5231019A (en) 1984-05-11 1993-07-27 Ciba-Geigy Corporation Transformation of hereditary material of plants
FI864720A7 (fi) * 1985-11-22 1987-05-23 Ciba Geigy Ag Suora geeninsiirto plastideihin ja mitokondrioihin.
ATE87032T1 (de) * 1986-12-05 1993-04-15 Ciba Geigy Ag Verbessertes verfahren zur transformation von pflanzlichen protoplasten.
ATE125418T1 (de) * 1987-05-20 1995-08-15 Ciba Geigy Ag Zeamays-pflanzen und transgenetische zeamays- pflanzen, die aus protoplasten oder aus von protoplasten erhaltenen zellen regeneriert wurden.
US5350689A (en) * 1987-05-20 1994-09-27 Ciba-Geigy Corporation Zea mays plants and transgenic Zea mays plants regenerated from protoplasts or protoplast-derived cells
US5128460A (en) * 1989-04-04 1992-07-07 Genelabs Incorporated Recombinant trichosanthin and coding sequence
CA2042093C (en) * 1990-05-09 2002-12-24 Gyula Hadlaczky Cell line carrying an excess of mammalian centromeres
US6605712B1 (en) * 1990-12-20 2003-08-12 Arch Development Corporation Gene transcription and ionizing radiation: methods and compositions
US5850025A (en) * 1991-09-19 1998-12-15 Sibia Neurosciences, Inc. Protection of plants against plant pathogens
US5422108A (en) * 1991-09-19 1995-06-06 Smart Plants International Inc. Protection of plants against plant pathogens
US5837851A (en) * 1994-12-08 1998-11-17 Pioneer Hi-Bred International, Inc. DNA promoter 5126 and constructs useful in a reversible nuclear genetic system for male sterility in transgenic plants
US5750868A (en) * 1994-12-08 1998-05-12 Pioneer Hi-Bred International, Inc. Reversible nuclear genetic system for male sterility in transgenic plants
EP0757102A1 (de) 1995-08-04 1997-02-05 Plant Genetic Systems N.V. Genetische Transformation durch Verwendung von einem PARP-Inhibitor
US6084164A (en) 1996-03-25 2000-07-04 Pioneer Hi-Bred International, Inc. Sunflower seeds with enhanced saturated fatty acid contents
US6025155A (en) * 1996-04-10 2000-02-15 Chromos Molecular Systems, Inc. Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes
US20020160970A1 (en) * 1996-04-10 2002-10-31 Gyula Hadlaczky Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes
US6077697A (en) * 1996-04-10 2000-06-20 Chromos Molecular Systems, Inc. Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes
US20030033617A1 (en) * 1996-04-10 2003-02-13 Gyula Hadlaczky Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes
NZ507093A (en) 1998-04-08 2003-08-29 Commw Scient Ind Res Org Methods and means for reducing the phenotypic expression of a nucleic acid of interest in a plant
CA2256121A1 (en) * 1998-06-08 1999-12-08 Hong Wang Cyclin-dependent kinase inhibitors as plant growth regulators
US6815579B1 (en) 1999-07-22 2004-11-09 The University Of British Columbia Plant long chain fatty acid biosynthetic enzyme
CA2284246A1 (en) 1999-10-01 2001-04-01 Agriculture And Agrifood Canada Of Agriculture And Agri-Food Plant fatty acid desaturases and alleles therefor
IL149459A0 (en) 1999-11-12 2002-11-10 Fibrogen Inc Recombinant gelatins
ES2321375T3 (es) 1999-12-28 2009-06-05 Bayer Bioscience N.V. Proteinas insecticidas de bacillus thuringiensis.
GB0002814D0 (en) 2000-02-09 2000-03-29 Univ York Nucleic acids and their uses
EP1366154A2 (de) 2000-09-15 2003-12-03 Her Majesty in right of Canada as represented by The Minister of Agricuture and Agri-Food Canada, Saskatoon Research Center Modulation der rekombination während der meiosis
US7517975B2 (en) 2000-09-26 2009-04-14 Pioneer Hi-Bred International, Inc. Nucleotide sequences mediating male fertility and method of using same
EP1911837B1 (de) * 2000-09-28 2011-05-25 Bioriginal Food & Science Corp. FAD5-2, Mitglied der Desaturase-Familie und Verwendungen davon
US8716022B2 (en) * 2000-11-17 2014-05-06 Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of Agriculture And Agri-Food Modulation of meiotic recombination
EP1988099B1 (de) * 2001-01-09 2012-11-14 Bayer CropScience NV Insektizide Proteine von Bacillus thuringiensis
WO2002057294A2 (en) * 2001-01-22 2002-07-25 Sangamo Biosciences, Inc. Zinc finger proteins for dna binding and gene regulation in plants
CA2340998C (en) 2001-03-21 2012-01-03 Saskatchewan Wheat Pool Selective modification of plant fatty acids
US20040242523A1 (en) * 2003-03-06 2004-12-02 Ana-Farber Cancer Institue And The Univiersity Of Chicago Chemo-inducible cancer gene therapy
US8034791B2 (en) 2001-04-06 2011-10-11 The University Of Chicago Activation of Egr-1 promoter by DNA damaging chemotherapeutics
ES2380007T3 (es) * 2001-04-06 2012-05-07 The University Of Chicago Inducción por agentes quimioterapéuticos de la actividad del promotor Egr-1 en la terapia génica
JP4338402B2 (ja) 2001-05-22 2009-10-07 ユニバーシティ オブ シカゴ N4ウイルス一本鎖dna依存的rnaポリメラーゼ
WO2002096923A1 (en) * 2001-05-30 2002-12-05 Chromos Molecular Systems, Inc. Plant artificial chromosomes, uses thereof and methods of preparing plant artificial chromosomes
AR037038A1 (es) 2001-10-26 2004-10-20 Baylor College Medicine Una composicion y metodo para alterar la masa corporal magra y las propiedades oseas en un sujeto
AU2002357143B2 (en) * 2001-12-11 2009-07-30 Advisys, Inc. Growth hormone releasing hormone supplementation for treating chronically ill subjects
US7262054B2 (en) * 2002-01-22 2007-08-28 Sangamo Biosciences, Inc. Zinc finger proteins for DNA binding and gene regulation in plants
US20060009409A1 (en) 2002-02-01 2006-01-12 Woolf Tod M Double-stranded oligonucleotides
US20030166282A1 (en) 2002-02-01 2003-09-04 David Brown High potency siRNAS for reducing the expression of target genes
EP2213737B1 (de) 2002-02-01 2012-11-07 Life Technologies Corporation Doppelsträngige Oligonukleotide
DE10212892A1 (de) 2002-03-20 2003-10-09 Basf Plant Science Gmbh Konstrukte und Verfahren zur Regulation der Genexpression
EP2213681A1 (de) 2002-03-22 2010-08-04 Bayer BioScience N.V. Neue insektizide Proteine von Bazillus Thuringiensis
US6916474B2 (en) 2002-07-15 2005-07-12 Board Of Regents, The University Of Texas System Antibodies with increased affinities for anthrax antigens
US20040268441A1 (en) 2002-07-19 2004-12-30 University Of South Carolina Compositions and methods for the modulation of gene expression in plants
ATE405658T1 (de) 2002-07-26 2008-09-15 Basf Plant Science Gmbh Neue selektionsverfahren
EP2269619A1 (de) 2002-08-12 2011-01-05 Jennerex Biotherapeutics ULC Verfahren und Zusammensetzungen in Bezug auf Pockenviren und Krebs
TW200424214A (en) * 2003-04-21 2004-11-16 Advisys Inc Plasmid mediated GHRH supplementation for renal failures
US20070224615A1 (en) * 2003-07-09 2007-09-27 Invitrogen Corporation Methods for assaying protein-protein interactions
JP4734498B2 (ja) 2003-07-09 2011-07-27 ライフ テクノロジーズ コーポレイション タンパク質−タンパク質相互作用を解析する方法
EP1502954A1 (de) * 2003-07-31 2005-02-02 greenovation Biotech GmbH Gebrauch von Konstrukten mit rekombinanten Sequenzmotifen zur Erhöhung der Genexpression in Moosen
IL157538A0 (en) 2003-08-21 2004-03-28 Bar Ilan Res & Dev Company Ltd Plant resistant to cytoplasm-feeding parasites
WO2005103254A1 (en) * 2004-04-22 2005-11-03 Yissum Research Development Company Of The Hebrew University Of Jerusalem UNIVERSAL TARGET SEQUENCES FOR siRNA GENE SILENCING
CA2857881A1 (en) 2004-11-12 2006-12-28 Asuragen, Inc. Methods and compositions involving mirna and mirna inhibitor molecules
EP1874936B1 (de) 2005-04-19 2013-10-30 BASF Plant Science GmbH Verbesserte verfahren zur kontrolle der genexpression
CN101300268A (zh) 2005-07-08 2008-11-05 墨西哥国立自治大学 新的具有杀虫活性的细菌蛋白质
KR101772375B1 (ko) * 2005-09-07 2017-08-29 신라젠(주) Gm-csf를 발현하는 폭스바이러스를 사용한 전이성 및/또는 전신 파종성 암의 전신 치료법
US8980246B2 (en) 2005-09-07 2015-03-17 Sillajen Biotherapeutics, Inc. Oncolytic vaccinia virus cancer therapy
EP2980220A1 (de) 2005-09-20 2016-02-03 BASF Plant Science GmbH Verbesserte verfahren zur steuerung der genexpression
US20090317421A1 (en) 2006-01-18 2009-12-24 Dominique Missiakas Compositions and methods related to staphylococcal bacterium proteins
PL1999141T3 (pl) 2006-03-21 2011-10-31 Bayer Cropscience Nv Nowe geny kodujące białka owadobójcze
US7666677B2 (en) 2006-07-05 2010-02-23 Luis Fabricio Medina-Bolivar Production of stilbenes in plant hairy root cultures
CA2663034C (en) 2006-09-15 2016-05-03 Ottawa Health Research Institute Oncolytic rhabdovirus
WO2008067547A2 (en) 2006-11-30 2008-06-05 Research Development Foundation Improved immunoglobulin libraries
CA2674499A1 (en) 2007-02-06 2008-08-14 Basf Plant Science Gmbh Use of alanine racemase genes to confer nematode resistance to plants
MX2009007608A (es) 2007-02-06 2009-07-27 Basf Plant Science Gmbh Composiciones y metodos que utilizan arn de interferencia para el control de nematodos.
CA2674562A1 (en) 2007-02-08 2008-08-14 Basf Plant Science Gmbh Compositions and methods using rna interference of opr3-like gene for control of nematodes
US20100017910A1 (en) 2007-02-09 2010-01-21 Basf Plant Science Gmbh Compositions and Methods Using RNA Interference of CDPK-Like For Control of Nematodes
KR20080084528A (ko) 2007-03-15 2008-09-19 제네렉스 바이오테라퓨틱스 인크. 종양살상형 백시니아 바이러스 암 치료
WO2008110522A1 (en) 2007-03-15 2008-09-18 Basf Plant Science Gmbh Use of nematode chitinase genes to control plant parasitic nematodes
WO2008118394A1 (en) * 2007-03-23 2008-10-02 New York University Methods of affecting nitrogen assimilation in plants
GB0709835D0 (en) 2007-05-22 2007-07-04 Plant Bioscience Ltd Composition and method for modulating plant root hair development
CN101688216B (zh) * 2007-06-01 2014-03-26 拜尔作物科学公司 编码杀虫蛋白质的新基因
CA2695530C (en) 2007-08-03 2016-07-05 Pioneer Hi-Bred International, Inc. Msca1 nucleotide sequences impacting plant male fertility and method of using same
EP2185190B1 (de) 2007-08-31 2015-06-24 University Of Chicago Verfahren und zusammensetzungen zur immunisierung gegen durch staphylokokken vermittelte lungenerkrankungen und -leiden
EP2554674B1 (de) 2007-12-21 2014-11-05 Keygene N.V. Trichomenspezifische Promotoren
CA2711499C (en) 2008-01-10 2018-09-25 Research Development Foundation Vaccines and diagnostics for the ehrlichioses
BRPI0911589A2 (pt) * 2008-05-01 2015-08-04 Bayer Bioscience Nv Manejo de resistência do inseto lagarta do cartucho em plantas transgênicas
CN101280315B (zh) * 2008-05-20 2010-09-01 中国农业科学院油料作物研究所 毛白杨木质素单体合成基因4-cl及应用
EP3279314A1 (de) 2008-06-04 2018-02-07 Cellular Dynamics International, Inc. Verfahren zur herstellung von ips-zellen unter verwendung eines nicht-viralen ansatzes
JP2012500005A (ja) 2008-08-12 2012-01-05 セルラー ダイナミクス インターナショナル, インコーポレイテッド iPS細胞を生成するための方法
EP2328603A4 (de) 2008-08-18 2013-01-02 Univ Maryland Apf-derivate und verwendungsverfahren
BRPI0917371A2 (pt) 2008-08-27 2015-11-17 Basf Plant Science Gmbh planta trangênica transformada com um vetor de expressão, semente, vetor de expressão, e, método para produzir uma planta trangênica resistente a nematóides.
GB2465748B (en) 2008-11-25 2012-04-25 Algentech Sas Plant cell transformation method
GB2465749B (en) 2008-11-25 2013-05-08 Algentech Sas Plant cell transformation method
EP2370568B1 (de) 2008-12-10 2017-07-19 Dana-Farber Cancer Institute, Inc. Mek mutanten mit resistenz gegen mek inhibitoren
DE112009003576T5 (de) 2008-12-11 2012-09-06 Basf Plant Science Gmbh Pflanzenwurzel-spezifische Nematodenresistenz
JP2012515532A (ja) 2009-01-20 2012-07-12 ラモット アット テル アビブ ユニバーシティ, リミテッド Mir−21プロモーター駆動性標的がん治療
GB2467167B (en) 2009-01-26 2013-09-11 Algentech Sas Gene targeting in plants
CA2754956A1 (en) 2009-03-20 2010-09-23 Basf Plant Science Company Gmbh Nematode-resistant transgenic plants
ES2784957T3 (es) 2009-04-03 2020-10-02 Univ Chicago Composiciones y métodos relacionados con variantes de la proteína A (SPA)
DK3061766T3 (da) 2009-04-28 2020-03-16 Univ Vanderbilt Sammensætninger og fremgangsmåder til behandlingen af sygdomme der involverer epitelcelleapoptose
DK2438160T3 (en) 2009-06-05 2016-01-11 Cellular Dynamics Int Inc Reprogramming of T cells and hematopoietic cells
CN102459615B (zh) 2009-06-08 2017-05-03 纽海姆有限公司 耐旱植物
CA2765357A1 (en) 2009-06-15 2010-12-23 Plant Bioscience Limited Production of viral capsids
US20120096600A1 (en) 2009-07-01 2012-04-19 Rene Ruiter Methods and means for obtaining plants with enhanced glyphosate tolerance
EP2462230B1 (de) * 2009-08-03 2015-07-15 Recombinetics, Inc. Verfahren und zusammensetzungen für gezielte genmodifikation
US20120131701A1 (en) 2009-08-05 2012-05-24 Chromocell Corporation Improved plants, microbes, and organisms
US20120151629A1 (en) 2009-08-25 2012-06-14 Basf Plant Science Company Gmbh Nematode-Resistant Transgenic Plants
BR112012005774B8 (pt) 2009-09-14 2023-02-14 Jennerex Inc uso de um inibidor de quinase antiangiogênico no tratamento de câncer
EP2357239A1 (de) 2009-10-29 2011-08-17 Universität zu Köln Verfahren und Mittel für ein auswählbares Markierungssystem bei Pflanzen
US8927812B2 (en) 2009-12-09 2015-01-06 Basf Plant Science Company Gmbh Methods for increasing the resistance of plants to fungi by silencing the fungal SMT1-gene
CA2836117C (en) 2009-12-10 2017-08-15 Ottawa Hospital Research Institute Oncolytic rhabdovirus
US10080799B2 (en) 2010-02-12 2018-09-25 Arizona Board Of Regents On Behalf Of Arizona State University Methods and compositions related to glycoprotein-immunoglobulin fusions
EP2539362A1 (de) 2010-02-23 2013-01-02 BASF Plant Science Company GmbH Nematodenresistente transgene pflanzen
EP3028699B1 (de) 2010-02-25 2018-03-21 Dana-Farber Cancer Institute, Inc. Braf-mutationen für resistenz gegen braf-inhibitoren
ES2631458T3 (es) 2010-03-04 2017-08-31 Interna Technologies B.V. Molécula de ARNmi definida por su fuente y sus usos terapéuticos en el cáncer asociado a la EMT
JP2013523818A (ja) 2010-04-05 2013-06-17 ザ・ユニバーシティー・オブ・シカゴ 免疫反応のエンハンサーとしてのプロテインA(SpA)抗体に関連する組成物および方法
WO2011126976A1 (en) 2010-04-07 2011-10-13 Vanderbilt University Reovirus vaccines and methods of use therefor
DK2560672T3 (en) 2010-04-19 2014-03-17 Res Dev Foundation RTEF-1 variants and their uses
GB201008720D0 (en) 2010-05-25 2010-07-07 Univ Aberdeen RxLR-leader peptides and protein translocation
CN103220905A (zh) 2010-05-28 2013-07-24 纽海姆有限公司 果实大小增大的植物
JP5908466B2 (ja) 2010-06-09 2016-04-26 デイナ ファーバー キャンサー インスティチュート,インコーポレイテッド Raf及びmek阻害剤に対する耐性を与えるmek1変異
JP5984217B2 (ja) 2010-06-15 2016-09-06 セルラー ダイナミクス インターナショナル, インコーポレイテッド 少量の末梢血からの人工多能性幹細胞の作製
JP6002128B2 (ja) 2010-07-02 2016-10-05 ザ・ユニバーシティ・オブ・シカゴThe University Of Chicago プロテインA(SpA)変種に関連する組成物および方法
NZ704322A (en) 2010-07-06 2016-07-29 Interna Technologies Bv Mirna and its diagnostic and therapeutic uses in diseases or conditions associated with melanoma, or in diseases or conditions associated with activated braf pathway
EP2590996B1 (de) 2010-07-08 2016-05-25 Bayer CropScience NV Glucosinolat-transporterprotein und verwendungen davon
WO2012018933A2 (en) 2010-08-04 2012-02-09 Cellular Dynamics International, Inc. Reprogramming immortalized b cells
US9095540B2 (en) 2010-09-09 2015-08-04 The University Of Chicago Methods and compositions involving protective staphylococcal antigens
EP3536337A1 (de) 2010-09-22 2019-09-11 The Regents of the University of Colorado, a body corporate Therapeutische anwendungen von smad7
CA2820706C (en) 2010-12-03 2018-05-22 Ms Technologies, Llc Optimized expression of glyphosate resistance encoding nucleic acid molecules in plant cells
CA2822004A1 (en) 2010-12-20 2012-06-28 Basf Plant Science Company Gmbh Nematode-resistant transgenic plants
KR101942237B1 (ko) 2011-01-04 2019-01-25 신라젠(주) 종양 항원에 대한 항체의 생산 및 종양용해 우두 바이러스의 투여에 의한 종양 특이적 보체 의존적 세포독성의 생산
EP2474617A1 (de) 2011-01-11 2012-07-11 InteRNA Technologies BV MIR zur Behandlung von Neoangiogenese
US8648230B2 (en) 2011-03-18 2014-02-11 Ms Technologies, Llc Regulatory regions preferentially expressing in non-pollen plant tissue
US9085631B2 (en) 2011-04-08 2015-07-21 Nov Vac APS Proteins and nucleic acids useful in vaccines targeting Staphylococcus aureus
US8945588B2 (en) 2011-05-06 2015-02-03 The University Of Chicago Methods and compositions involving protective staphylococcal antigens, such as EBH polypeptides
EP2535416A1 (de) 2011-05-24 2012-12-19 BASF Plant Science Company GmbH Entwicklung einer Phytophthora-resistenten Kartoffel mit verbessertem Ertrag
US9234193B2 (en) 2011-05-31 2016-01-12 Keygene N.V. Pest resistant plants
CA2872045A1 (en) 2011-06-08 2012-12-13 Children's Hospital Of Eastern Ontario Research Institute Inc. Compositions and methods for glioblastoma treatment
BR112014003315A2 (pt) 2011-08-15 2017-03-01 Univ Chicago composições e métodos relacionados a anticorpos para a proteína a estafilocócica
WO2013026015A1 (en) 2011-08-18 2013-02-21 Dana-Farber Cancer Institute, Inc. Muc1 ligand traps for use in treating cancers
WO2013053899A1 (en) 2011-10-12 2013-04-18 Moeller Niels Iversen Peptides derived from campylobacter jejuni and their use in vaccination
EP2768955A1 (de) 2011-10-19 2014-08-27 Keygene N.V. Verfahren und zusammensetzungen zur herstellung drimenol
ES2886147T3 (es) 2011-12-22 2021-12-16 Interna Tech B V MiARN para el tratamiento del cáncer de cabeza y de cuello
US9644213B2 (en) 2012-03-09 2017-05-09 Board Of Trustees Of Michigan State University Method of enhancing plant drought tolerance by expression of NDR1
EP2841101B1 (de) 2012-04-26 2019-08-07 University Of Chicago Zusammensetzungen und verfahren in zusammenhang mit antikörpern zur neutralisierung der koagulaseaktivität bei einer staphylococcus-aureus-erkrankung
AU2013252883B2 (en) 2012-04-26 2018-05-17 University Of Chicago Staphylococcal coagulase antigens and methods of their use
RU2014151361A (ru) 2012-05-18 2016-07-10 Пайонир Хай-Бред Интернэшнл, Инк. Последовательности индуцируемого промотора для регулируемой экспрессии и способы применения
US10201556B2 (en) 2012-11-06 2019-02-12 Interna Technologies B.V. Combination for use in treating diseases or conditions associated with melanoma, or treating diseases or conditions associated with activated B-raf pathway
US9598707B2 (en) 2012-11-26 2017-03-21 Arkansas State University-Jonesboro Method to increase the yield of products in plant material
US10125373B2 (en) 2013-01-22 2018-11-13 Arizona Board Of Regents On Behalf Of Arizona State University Geminiviral vector for expression of rituximab
CA2897485A1 (en) 2013-01-29 2014-08-07 Basf Plant Science Company Gmbh Fungal resistant plants expressing hcp6
US10462994B2 (en) 2013-01-29 2019-11-05 Basf Plant Science Company Gmbh Fungal resistant plants expressing HCP7
US10231397B2 (en) 2013-01-29 2019-03-19 Basf Plant Science Company Gmbh Fungal resistant plants expressing EIN2
CA2901501C (en) 2013-02-21 2023-03-07 Children's Hospital Of Eastern Ontario Research Institute Inc. Vaccine composition
US9783817B2 (en) 2013-03-04 2017-10-10 Arkansas State University Methods of expressing and detecting activity of expansin in plant cells
CA2900005A1 (en) 2013-03-08 2014-09-12 Basf Plant Science Company Gmbh Fungal resistant plants expressing mybtf
CN105358704B (zh) 2013-03-08 2020-01-03 科罗拉多大学董事会 Ptd-smad7治疗剂
WO2014142647A1 (en) 2013-03-14 2014-09-18 Wageningen Universiteit Fungals strains with improved citric acid and itaconic acid production
EA033454B1 (ru) 2013-08-14 2019-10-31 Inst Of Genetics And Developmental Biology Способы модулирования размера семян и органов у растений
EP3043825A4 (de) 2013-09-09 2017-05-03 Figene, LLC Gentherapie zur regeneration von chondrozyten oder knorpelzellen
EP3065706A4 (de) 2013-11-08 2017-11-29 Baylor Research Institute Nukleare lokalisierung von glp-1 zur stimulation der herzmuskelregenerierung und reversion von herzversagen
CA2965327C (en) 2013-11-08 2023-05-09 The Board Of Regents Of The University Of Texas System Vh4 antibodies against gray matter neuron and astrocyte
GB201319876D0 (en) 2013-11-11 2013-12-25 Plant Bioscience Ltd Methods of modulating seed and organ size in plants
ES3012932T3 (en) 2013-12-03 2025-04-10 Evaxion Biotech As Proteins and nucleic acids useful in vaccines targeting staphylococcus aureus
US9714429B2 (en) 2014-01-28 2017-07-25 Arkansas State University Regulatory sequence of cupin family gene
US10059775B2 (en) 2014-01-29 2018-08-28 Dana-Farber Cancer Institute, Inc. Antibodies against the MUC1-C/extracellular domain (MUC1-C/ECD)
US10214741B2 (en) 2014-02-14 2019-02-26 University Of Utah Research Foundation Methods and compositions for inhibiting retinopathy of prematurity
WO2015130783A1 (en) 2014-02-25 2015-09-03 Research Development Foundation Sty peptides for inhibition of angiogenesis
US20180169211A1 (en) 2014-11-13 2018-06-21 Evaxion Biotech Aps Peptides derived from acinetobacter baumannii and their use in vaccination
WO2016113252A2 (en) 2015-01-12 2016-07-21 Evaxion Biotech Aps Proteins and nucleic acids useful in vaccines targeting klebsiella pneumoniae
EP3250675A1 (de) 2015-01-28 2017-12-06 SABIC Global Technologies B.V. Verfahren und zusammensetzungen zum hocheffizienten herstellung von biokraftstoff und/oder biomasse
BR112017016688B1 (pt) 2015-02-04 2024-01-23 Basf Plant Science Company Gmbh Método para aumentar a resistência fúngica, método para a produção de um produto e método para criar uma planta resistente a fungos
EP3259346B1 (de) 2015-02-20 2024-08-07 Baylor College of Medicine P63-inaktivierung zur behandlung von herzinsuffizienz
EP3317295B1 (de) 2015-07-04 2022-05-18 Evaxion Biotech A/S Proteine und nukleinsäuren, die in impfstoffen gegen pseudomonas aeruginosa nützlich sind
IL257831B2 (en) 2015-09-04 2025-11-01 Keygene Nv Diplosporic garden
WO2017053433A1 (en) 2015-09-21 2017-03-30 Modern Meadow, Inc. Fiber reinforced tissue composites
MX2018005274A (es) 2015-10-30 2019-09-19 The Regents Of The Universtiy Of California Metodos para la generacion de celulas-t a partir de celulas madre y metodos inmunoterapeuticos que utilizan las celulas-t.
JP6987068B2 (ja) 2015-11-09 2021-12-22 ザ・チルドレンズ・ホスピタル・オブ・フィラデルフィアThe Children’S Hospital Of Philadelphia 癌マーカーおよび治療標的としてのグリピカン2
EP3205666B1 (de) 2016-02-15 2020-04-22 Modern Meadow, Inc. Verfahren zur herstellung eines biologisch hergestellten materials mit kollagenfibrillen
EP3419654B1 (de) 2016-02-22 2022-04-27 Evaxion Biotech A/S In impfstoffen gegen staphylococcus aureus nützliche proteine und nukleinsäuren
AU2017232723B2 (en) 2016-03-15 2023-04-20 National Research Council Of Canada Modulating plant abiotic stress responses using the kanghan gene family
WO2017168348A1 (en) 2016-03-31 2017-10-05 Baylor Research Institute Angiopoietin-like protein 8 (angptl8)
US10563216B2 (en) 2016-04-18 2020-02-18 Bloomsburg University of Pennsylvania Compositions and methods of delivering molecules to plants
EP3054014A3 (de) 2016-05-10 2016-11-23 BASF Plant Science Company GmbH Benutzung einer fungizids auf transgenen pflanzen
CN109689089A (zh) 2016-05-25 2019-04-26 国家医疗保健研究所 治疗癌症的方法和组合物
WO2017216384A1 (en) 2016-06-17 2017-12-21 Evaxion Biotech Aps Vaccination targeting ichthyophthirius multifiliis
EP3260542A1 (de) 2016-06-20 2017-12-27 Algentech Proteinproduktion in pflanzenzellen
JP7075900B2 (ja) 2016-06-20 2022-05-26 ボード オブ スーパーバイザーズ オブ ルイジアナ ステート ユニバーシティ アンド アグリカルチュアル アンド メカニカル カレッジ 緑藻重炭酸輸送体およびその用途
WO2017220787A1 (en) 2016-06-24 2017-12-28 Evaxion Biotech Aps Vaccines against aearomonas salmonicida infection
US11414464B2 (en) 2016-07-22 2022-08-16 Evaxion Biotech A/S Chimeric proteins for inducing immunity towards infection with S. aureus
JP2019530440A (ja) 2016-09-02 2019-10-24 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア インターロイキン6受容体α結合単鎖可変断片を含む方法および組成物
US11718648B2 (en) 2017-01-05 2023-08-08 Evaxion Biotech A/S Vaccines targeting Pseudomonas aeruginosa
US12534736B2 (en) 2017-03-07 2026-01-27 The United States Of America, As Represented By The Secretary Of Agriculture Plants with increased photorespiration efficiency
WO2018213679A1 (en) 2017-05-19 2018-11-22 Second Genome, Inc. Proteins for the treatment of epithelial barrier function disorders
CA3008850A1 (en) 2017-06-29 2018-12-29 Modern Meadow, Inc. Yeast strains and methods for producing collagen
US11497762B2 (en) 2017-11-03 2022-11-15 Interna Technologies B.V. MiRNA molecule, equivalent, antagomir, or source thereof for treating and/or diagnosing a condition and/or a disease associated with neuronal deficiency or for neuronal (re)generation
AU2018253595A1 (en) 2017-11-13 2019-05-30 Modern Meadow, Inc. Biofabricated leather articles having zonal properties
EP3710014A1 (de) 2017-11-14 2020-09-23 Henry Ford Health System Zusammensetzungen zur verwendung bei der behandlung und vorbeugung von kardiovaskulären störungen infolge von zerebrovaskulären verletzungen
KR20250008153A (ko) 2017-11-24 2025-01-14 인쎄름 (엥스띠뛰 나씨오날 드 라 쌍떼 에 드 라 흐쉐르슈 메디깔) 암 치료를 위한 방법 및 조성물
BR112020013715A2 (pt) 2018-01-05 2020-12-01 Ottawa Hospital Research Institute vetores de vaccinia modificados
WO2019145399A1 (en) 2018-01-24 2019-08-01 Evaxion Biotech Aps Vaccines for prophylaxis of s. aureus infections
WO2019185129A1 (en) 2018-03-27 2019-10-03 The University Court Of The University Of Glasgow Bacterial pathogen derived resistance in plants
CN112601452A (zh) 2018-07-13 2021-04-02 路易斯安那州立大学监事会和农机学院 绿藻的bestrophin碳酸氢盐转运蛋白
CN120866381A (zh) 2018-08-13 2025-10-31 奥尔胡斯大学 表达识别脂壳寡糖的异源受体的基因改变的植物
US12190997B2 (en) 2018-08-13 2025-01-07 Aarhus Universitet Genetically altered LysM receptors with altered agonist specificity and affinity
WO2020069313A2 (en) 2018-09-28 2020-04-02 Henry Ford Health System Use of extracellular vesicles in combination with tissue plasminogen activator and/or thrombectomy to treat stroke
EP3870207A1 (de) 2018-10-22 2021-09-01 Evaxion Biotech ApS Impfstoff gegen m. catharrhalis
CN113544276A (zh) 2018-12-06 2021-10-22 瓦赫宁根大学 基因改变植物nin-基因以对细胞分裂素应答的方法
AU2020209847B2 (en) 2019-01-17 2024-10-17 Modern Meadow, Inc. Layered collagen materials and methods of making the same
WO2020174044A1 (en) 2019-02-27 2020-09-03 Evaxion Biotech Aps Vaccines targeting h. influenzae
EP3942052A1 (de) 2019-03-21 2022-01-26 University of Essex Enterprises Limited Verfahren zur erhöhung der biomasse in einer pflanze durch stimulation der rubp-regeneration und des elektronentransports
MX2021013833A (es) 2019-05-14 2022-03-17 Univ Chicago Metodos y composiciones que comprenden variantes de la proteina a de estafilococos (spa).
CN113874388B (zh) 2019-05-29 2025-09-16 主基因有限公司 孤雌生殖基因
EP3997111A4 (de) 2019-07-11 2023-07-26 The Regents Of The University Of California Verfahren zur verbesserten regeneration transgener pflanzen mit wachstumsregulierendem faktor (grf), grf-interaktionsfaktor (gif) oder chimären grf-gif-genen und proteinen
CN120484131A (zh) 2019-07-19 2025-08-15 费城儿童医院 包含磷脂酰肌醇蛋白聚糖2结合结构域的嵌合抗原受体
BR112022001945A2 (pt) 2019-08-02 2022-05-03 Hughes Howard Med Inst Motivos proteicos de ligação à rubisco e usos dos mesmos
GB201911068D0 (en) 2019-08-02 2019-09-18 Univ Edinburgh Modified higher plants
WO2021032769A1 (en) 2019-08-19 2021-02-25 Aarhus Universitet Modified exopolysaccharide receptors for recognizing and structuring microbiota
TW202128160A (zh) 2019-10-18 2021-08-01 美國加利福尼亞大學董事會 用於治療病原性血管病症之方法及組合物
EP4087593A1 (de) 2020-01-06 2022-11-16 Evaxion Biotech A/S Impfstoffe, die gegen neisseria gonorrhoeae gerichtet sind
EP4110928A1 (de) 2020-02-28 2023-01-04 Cambridge Enterprise Limited Verfahren, pflanzen und zusammensetzungen zum überwinden der nährstoffunterdrückung der mykorrhiza-symbiose
WO2021194343A1 (en) 2020-03-25 2021-09-30 Erasmus University Medical Center Rotterdam Reporter system for radionuclide imaging
US20210355326A1 (en) 2020-05-01 2021-11-18 Modern Meadow, Inc. Protein polyurethane alloys and layered materials including the same
CN116323952A (zh) 2020-05-19 2023-06-23 奥尔胡斯大学 Lysm受体基序
WO2022053130A1 (en) 2020-09-09 2022-03-17 Sid Alex Group, S.R.O. Antago-mir-155 for treatment of v-src, c-src-tyrosine kinase-induced cancers
CN116568131B (zh) 2020-10-13 2026-01-06 主基因有限公司 单性生殖基因的修饰的启动子
JP2023548556A (ja) 2020-11-05 2023-11-17 ボード オブ リージェンツ,ザ ユニバーシティ オブ テキサス システム Egfr抗原を標的とする操作されたt細胞受容体および使用方法
US20240123004A1 (en) 2021-02-09 2024-04-18 University Of Houston System Oncolytic virus for systemic delivery and enhanced anti-tumor activities
WO2022175815A1 (en) 2021-02-19 2022-08-25 Pfizer Inc. Methods of protecting rna
BR112023024446A2 (pt) 2021-05-26 2024-02-06 Univ Illinois Plantas c4 com aumento de eficiência fotossintética
WO2022256695A1 (en) 2021-06-03 2022-12-08 Mazen Animal Health Inc. Oral administration of coronavirus spike protein for altering cytokine levels and providing passive immunity to newborn pigs
WO2023280807A1 (en) 2021-07-05 2023-01-12 Evaxion Biotech A/S Vaccines targeting neisseria gonorrhoeae
WO2023089556A1 (en) 2021-11-22 2023-05-25 Pfizer Inc. Reducing risk of antigen mimicry in immunogenic medicaments
GB2614309A (en) 2021-12-24 2023-07-05 Stratosvir Ltd Improved vaccinia virus vectors
EP4469079A1 (de) 2022-01-28 2024-12-04 Pfizer Inc. Coronavirus-antigenvarianten
EP4493213A1 (de) 2022-03-16 2025-01-22 University of Houston System System zur persistenten hsv-genabgabe
US20230323480A1 (en) 2022-04-11 2023-10-12 The Regents Of The University Of California Methods of screening for plant gain of function mutations and compositions therefor
JP2025516318A (ja) 2022-05-04 2025-05-27 エヴァクシオン・バイオテック・アクティエセルスカブ ブドウ球菌タンパク質変異体およびトランケート
EP4634214A1 (de) 2022-12-15 2025-10-22 Aarhus Universitet Synthetische aktivierung von multimeren transmembranrezeptoren
WO2024130212A1 (en) 2022-12-16 2024-06-20 Turnstone Biologics Corp. Recombinant vaccinia virus encoding one or more natural killer cell and t lymphocyte inhibitors
EP4658671A2 (de) 2023-02-03 2025-12-10 Aarhus Universitet Verbesserung der stickstofffixierung mit fund
WO2024186630A1 (en) 2023-03-03 2024-09-12 Henry Ford Health System Use of extracellular vesicles for the treatment of cancer
US20240344078A1 (en) 2023-03-14 2024-10-17 Aarhus Universitet Genetically altered nfr5 receptor kinases
AU2024237471A1 (en) 2023-03-14 2025-09-04 Aarhus Universitet Genetically altered nfr1 receptor kinases
AU2024268293A1 (en) 2023-05-05 2025-11-06 The Board Of Trustees Of The University Of Illinois Modified upstream open reading frames for modulating npq relaxation
EP4724466A1 (de) 2023-06-09 2026-04-15 Aarhus Universitet Symrk-phosphorylierung zur wurzelknötchenorganogenese
US20250092418A1 (en) 2023-08-28 2025-03-20 Niab Methods of genetically altering a plant nin-gene to be responsive to auxin
WO2025050009A2 (en) 2023-09-01 2025-03-06 Children's Hospital Medical Center Identification of targets for immunotherapy in melanoma using splicing-derived neoantigens
US20250361514A1 (en) 2024-05-24 2025-11-27 University of Kaiserslautern-Landau Engineering cassava for improved growth and yield
WO2026006779A1 (en) 2024-06-27 2026-01-02 The Regents Of The University Of California Transgenic plants expressing fern rapid non-photochemical quenching (npq) proteins

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4394448A (en) * 1978-02-24 1983-07-19 Szoka Jr Francis C Method of inserting DNA into living cells

Also Published As

Publication number Publication date
US4684611A (en) 1987-08-04
NL8200523A (nl) 1983-09-01
JPS58146282A (ja) 1983-08-31
EP0086537A1 (de) 1983-08-24
EP0086537B1 (de) 1987-05-27
JPH0630589B2 (ja) 1994-04-27
DE3371792D1 (en) 1987-07-02

Similar Documents

Publication Publication Date Title
ATE27463T1 (de) Verfahren zur in vitro transformation von pflanzenprotoplasten mit plasmid-dns.
AU560340B2 (en) Rennin from recombinant dna
ES2120946T3 (es) Sistemas y medios de cultivo celular.
BG60053B2 (bg) Метод за повишаване способността за получаване на антибиотик
DE69637656D1 (de) Methoden zur Züchtung von Tierzellen
ATE153214T1 (de) Medien zur zellzucht und verfahren dazu
ATE309267T1 (de) Methode zur proliferation und differenzierung von b-zellen und verwendungen dafür
ATE181479T1 (de) Verfahren zur entwicklung von neuen pflanzentypen mit kapazität zur stickstoffbindung ebenfalls in deren blättern
Buczek et al. The influence of gibberellic acid and kinetin on the growth of Scenedesmus quadricauda (Turp.) Breb.
ES8800723A1 (es) Un metodo para preparar renina humana.
AU583795B2 (en) Process for the maintenance and proliferation of virus genomes and derivatives thereof
AU581819B2 (en) Process for the immortalization of human and animal cells
JPS5783279A (en) Cultivation of microscopic green alga
AU6262786A (en) Growing aids virus in cell lines lacking human class ii histocompatability antigens
Gagnon et al. Autodegradation of RNA of plant cell ribosomes
DE69021005D1 (de) Verfahren zur Kultur von Tomaten-Protoplasten.
DE3871322D1 (de) Verfahren zur kultur von pflanzengewebe.
DE69923595D1 (de) Herstellung von proteinen
EP0166130A3 (de) Zusätze für Zellkultur, Kulturmedium für serumlose Kultur und Verfahren zur Zellkultur, die sie benutzen
ES542143A0 (es) Procedimiento para obtener un cultivo celular
JPS5596091A (en) Production of cells of actinomycetes microorganism
ATE49777T1 (de) Verfahren zur zuechtung von hepatozyten und cozuechtung von hepatozyten und von behandelten epithelien leberzellen.
KR890013189A (ko) 지치속 식물의 조직배양에 의한 시코닌 유도체의 제조방법
SU1692145A1 (ru) Способ получения клеточной культуры

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
REN Ceased due to non-payment of the annual fee