ATE276372T1 - Pichia methanolica glyceraldehyd-3-phosphat- dehydrogenase-1-promoter und -terminator - Google Patents

Pichia methanolica glyceraldehyd-3-phosphat- dehydrogenase-1-promoter und -terminator

Info

Publication number
ATE276372T1
ATE276372T1 AT00944702T AT00944702T ATE276372T1 AT E276372 T1 ATE276372 T1 AT E276372T1 AT 00944702 T AT00944702 T AT 00944702T AT 00944702 T AT00944702 T AT 00944702T AT E276372 T1 ATE276372 T1 AT E276372T1
Authority
AT
Austria
Prior art keywords
terminator
promoter
phosphate dehydrogenase
pichia methanolica
gap1
Prior art date
Application number
AT00944702T
Other languages
English (en)
Inventor
Brady G Miller
James S Sloan
Christopher K Raymond
Erica Vanaja
Original Assignee
Zymogenetics Inc
Brady G Miller
James S Sloan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zymogenetics Inc, Brady G Miller, James S Sloan filed Critical Zymogenetics Inc
Application granted granted Critical
Publication of ATE276372T1 publication Critical patent/ATE276372T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
AT00944702T 1999-06-24 2000-06-16 Pichia methanolica glyceraldehyd-3-phosphat- dehydrogenase-1-promoter und -terminator ATE276372T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14070399P 1999-06-24 1999-06-24
PCT/US2000/016671 WO2000078978A1 (en) 1999-06-24 2000-06-16 Pichia methanolica glyceraldehyde 3-phosphate dehydrogenase 1 promoter and terminator

Publications (1)

Publication Number Publication Date
ATE276372T1 true ATE276372T1 (de) 2004-10-15

Family

ID=22492440

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00944702T ATE276372T1 (de) 1999-06-24 2000-06-16 Pichia methanolica glyceraldehyd-3-phosphat- dehydrogenase-1-promoter und -terminator

Country Status (11)

Country Link
US (1) US6613547B1 (de)
EP (1) EP1192263B1 (de)
JP (1) JP2003503030A (de)
AT (1) ATE276372T1 (de)
AU (1) AU5876300A (de)
CA (1) CA2397494A1 (de)
DE (1) DE60013829T2 (de)
DK (1) DK1192263T3 (de)
ES (1) ES2231217T3 (de)
MX (1) MXPA02000160A (de)
WO (1) WO2000078978A1 (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4464269B2 (ja) 2002-04-26 2010-05-19 協和発酵キリン株式会社 哺乳類型糖鎖生産メチロトロフ酵母
US7259255B2 (en) * 2003-06-25 2007-08-21 E. I. Du Pont De Nemours And Company Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast
US20060166236A1 (en) * 2004-12-15 2006-07-27 Chong-Sheng Yuan Allosteric enzyme coupled immunoassay (AECIA)

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2000101A1 (en) * 1988-12-22 1990-06-22 Mary E. Digan Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase gene
DE69133066T2 (de) * 1990-01-16 2003-03-06 Centro De Ingenieria Genetica Y Biotecnologia, Cubanacan Playa Verfahren zur Expression heterologischer Gene in der Hefe Pichia Pastoris, Expressionsverfahren und transformierte Mikroorganismen
US5965389A (en) * 1995-11-09 1999-10-12 Zymogenetics, Inc. Production of GAD65 in methylotrophic yeast
MX9605082A (es) * 1996-10-24 1998-04-30 Univ Autonoma De Nuevo Leon Levaduras metilotroficas modificadas geneticamente para la produccion y secrecion de hormona de crecimiento humano.
WO1999014347A1 (en) * 1997-09-15 1999-03-25 Zymogenetics, Inc. PROTEASE-DEFICIENT STRAINS OF $i(PICHIA METHANOLICA)
US6258559B1 (en) * 1999-03-22 2001-07-10 Zymogenetics, Inc. Method for producing proteins in transformed Pichia

Also Published As

Publication number Publication date
DK1192263T3 (da) 2005-01-31
US6613547B1 (en) 2003-09-02
DE60013829T2 (de) 2005-09-22
DE60013829D1 (en) 2004-10-21
MXPA02000160A (es) 2003-10-14
AU5876300A (en) 2001-01-09
JP2003503030A (ja) 2003-01-28
EP1192263A1 (de) 2002-04-03
WO2000078978A1 (en) 2000-12-28
ES2231217T3 (es) 2005-05-16
EP1192263B1 (de) 2004-09-15
CA2397494A1 (en) 2000-12-28

Similar Documents

Publication Publication Date Title
ATE352626T1 (de) Polypeptide mit galaktoseoxidaseaktivität und dafür kodierende nukleisäuren
DE602004028724D1 (de) Verfahren zur herstellung sezernierter polypeptide
WO2000056900A3 (en) Promoter sequences derived from fusarium venenatum and uses thereof
WO2003089598A3 (en) Polypeptides having xyloglucanase activity and nucleic acids encoding same
ZA967173B (en) Purified coprinus laccases and nucleic acids and encoding same
DE60043428D1 (de) POLYPEPTIDE MIT VERZWEIGUNGSENZYM-AKTIVITäT UND FüR DIESE KODIERENDE NUKLEINSäUREN
ATE486941T1 (de) Corynebacterium glutamicum gene, die für stoffwechselproteine kodieren
DK1332222T3 (da) Stofskifteændringer i fermenteringer der udtrykker rekombinante proteiner
ATE384787T1 (de) Tripeptidyl-aminopeptidase
DE60137829D1 (de) Menschliche stra6 polypeptide
DE69837475D1 (de) Polypeptide mit aminopeptidaseaktivität und dafür kodierende nukleinsäuren
AU2003294397A8 (en) Promoter variants for expressing genes in a fungal cell
ATE395424T1 (de) Verfahren zur herstellung von heterologen polypeptiden in trichothecene mangelmutanten filamentlösen pilzen
CN102533839B (zh) 融合表达含有二硫键蛋白质的方法
ES2140398T3 (es) Produccion de factor xiii a por tecnologia genetica.
EP1534835A4 (de) Yapsin-mangelmutantenstamm von hansenula polymorpha sowie verfahren zur herstellung rekombinanter proteine damit
ATE383420T1 (de) Verwendung des cyp52a2a promotors für die erhöhung der genexpression in hefe
BR0108883A (pt) Sequência de ácido nucleico isolado, construção de ácido nucleico, vetor, microorganismo recombinante, e, processo para a preparação de ácidos carboxìlicos quirais ou aquirais
ATE276372T1 (de) Pichia methanolica glyceraldehyd-3-phosphat- dehydrogenase-1-promoter und -terminator
DE69934017D1 (de) 2,6-beta-d-fruktanhydrolase enzym und verfahren zu dessen verwendung
IL144648A0 (en) Heat-inducible promoter
FR2785291B1 (fr) Procede de production in vivo de proteines chimiquement diversifiees par incorporation d'acides amines non conventionnels
ATE317896T1 (de) Polypeptide mit glucanotransferase aktivität und dafür kodierende nukleinsäuren
AU2001263920A1 (en) Method for the production of proteins in a yeast of the genusarxula and suitable promoters
EP1721976A4 (de) Modifizierter promotor

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 1192263

Country of ref document: EP

REN Ceased due to non-payment of the annual fee