ATE278036T1 - Verfahren zur analyse der dna-methylierung unter verwendung von fluoreszenzpolarisation - Google Patents
Verfahren zur analyse der dna-methylierung unter verwendung von fluoreszenzpolarisationInfo
- Publication number
- ATE278036T1 ATE278036T1 AT02704676T AT02704676T ATE278036T1 AT E278036 T1 ATE278036 T1 AT E278036T1 AT 02704676 T AT02704676 T AT 02704676T AT 02704676 T AT02704676 T AT 02704676T AT E278036 T1 ATE278036 T1 AT E278036T1
- Authority
- AT
- Austria
- Prior art keywords
- oligonucleotide
- genomic dna
- pair
- fluorescent label
- chemically treated
- Prior art date
Links
- 238000002875 fluorescence polarization Methods 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 230000007067 DNA methylation Effects 0.000 title 1
- 108091034117 Oligonucleotide Proteins 0.000 abstract 5
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 abstract 4
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 abstract 2
- 229940104302 cytosine Drugs 0.000 abstract 2
- 239000007850 fluorescent dye Substances 0.000 abstract 2
- 230000011987 methylation Effects 0.000 abstract 2
- 238000007069 methylation reaction Methods 0.000 abstract 2
- LRSASMSXMSNRBT-UHFFFAOYSA-N 5-methylcytosine Chemical compound CC1=CNC(=O)N=C1N LRSASMSXMSNRBT-UHFFFAOYSA-N 0.000 abstract 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 239000007795 chemical reaction product Substances 0.000 abstract 1
- 229940035893 uracil Drugs 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10104937A DE10104937B4 (de) | 2001-01-29 | 2001-01-29 | Fluoreszenzpolarisation 2 |
| PCT/EP2002/000922 WO2002061123A2 (en) | 2001-01-29 | 2002-01-29 | Method of analysing dna methylation using fluorescence polarisation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE278036T1 true ATE278036T1 (de) | 2004-10-15 |
Family
ID=7672767
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT02704676T ATE278036T1 (de) | 2001-01-29 | 2002-01-29 | Verfahren zur analyse der dna-methylierung unter verwendung von fluoreszenzpolarisation |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20040121359A1 (de) |
| EP (1) | EP1390529B1 (de) |
| AT (1) | ATE278036T1 (de) |
| AU (1) | AU2002238524A1 (de) |
| DE (2) | DE10104937B4 (de) |
| WO (1) | WO2002061123A2 (de) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10104938B4 (de) * | 2001-01-29 | 2005-06-23 | Epigenomics Ag | Fluoreszenzpolarisation 1 |
| DE10245779A1 (de) * | 2002-10-01 | 2004-04-29 | Epigenomics Ag | Verfahren und Nukleinsäuren für die verbesserte Behandlung von proliferativen Erkrankungen von Brustzellen |
| US7534873B2 (en) * | 2003-08-29 | 2009-05-19 | Applied Biosystems, Llc | Method and materials for quaternary amine catalyzed bisulfite conversion of cytosine to uracil |
| US7368239B2 (en) * | 2003-08-29 | 2008-05-06 | Applera Corporation | Method and materials for polyamine catalyzed bisulfite conversion of cytosine to uracil |
| US7371526B2 (en) * | 2003-08-29 | 2008-05-13 | Applera Corporation | Method and materials for bisulfite conversion of cytosine to uracil |
| WO2006034264A2 (en) * | 2004-09-21 | 2006-03-30 | Applera Corporation | Methods of using sulfur nucleophiles as improved alternatives to sodium bisulfite for methylated dna analysis |
| US7658288B2 (en) * | 2004-11-08 | 2010-02-09 | Applied Biosystems, Llc | Bisulfite conversion reagent |
| CN102618645B (zh) * | 2012-03-30 | 2014-07-09 | 中国人民解放军第四军医大学 | 基于荧光偏振的egfr基因启动子甲基化状态均相检测法 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB8902689D0 (en) * | 1989-02-07 | 1989-03-30 | Ici Plc | Assay method |
| US6059724A (en) * | 1997-02-14 | 2000-05-09 | Biosignal, Inc. | System for predicting future health |
| JP3948503B2 (ja) * | 1997-08-01 | 2007-07-25 | 誠 鶴岡 | 蛍光偏光法による核酸の測定方法およびＶｅｒｏ毒素生産菌の検出方法 |
| DE19754482A1 (de) * | 1997-11-27 | 1999-07-01 | Epigenomics Gmbh | Verfahren zur Herstellung komplexer DNA-Methylierungs-Fingerabdrücke |
| US6180408B1 (en) * | 1998-08-21 | 2001-01-30 | Washington University | Fluorescence polarization in nucleic acid analysis |
| US6331393B1 (en) * | 1999-05-14 | 2001-12-18 | University Of Southern California | Process for high-throughput DNA methylation analysis |
-
2001
- 2001-01-29 DE DE10104937A patent/DE10104937B4/de not_active Expired - Fee Related
-
2002
- 2002-01-29 AT AT02704676T patent/ATE278036T1/de not_active IP Right Cessation
- 2002-01-29 EP EP02704676A patent/EP1390529B1/de not_active Expired - Lifetime
- 2002-01-29 AU AU2002238524A patent/AU2002238524A1/en not_active Abandoned
- 2002-01-29 US US10/470,698 patent/US20040121359A1/en not_active Abandoned
- 2002-01-29 DE DE60201441T patent/DE60201441T2/de not_active Expired - Lifetime
- 2002-01-29 WO PCT/EP2002/000922 patent/WO2002061123A2/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| DE10104937A1 (de) | 2002-08-14 |
| US20040121359A1 (en) | 2004-06-24 |
| WO2002061123A2 (en) | 2002-08-08 |
| WO2002061123A3 (en) | 2003-12-04 |
| DE60201441T2 (de) | 2005-10-27 |
| DE60201441D1 (de) | 2004-11-04 |
| DE10104937B4 (de) | 2005-03-17 |
| AU2002238524A1 (en) | 2002-08-12 |
| EP1390529A2 (de) | 2004-02-25 |
| EP1390529B1 (de) | 2004-09-29 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |