ATE280234T1 - Expression eines fusionspolypeptides, das ohne leadersequenz aus dem cytoplasma transportiert wird - Google Patents

Expression eines fusionspolypeptides, das ohne leadersequenz aus dem cytoplasma transportiert wird

Info

Publication number
ATE280234T1
ATE280234T1 AT94925175T AT94925175T ATE280234T1 AT E280234 T1 ATE280234 T1 AT E280234T1 AT 94925175 T AT94925175 T AT 94925175T AT 94925175 T AT94925175 T AT 94925175T AT E280234 T1 ATE280234 T1 AT E280234T1
Authority
AT
Austria
Prior art keywords
polypeptide
fusion
cytoplasm
expression
leader sequence
Prior art date
Application number
AT94925175T
Other languages
English (en)
Inventor
Desmond Mascarenhas
Yang Zhang
Pamela S Olson
David R Olsen
Pedro A Carrillo
Original Assignee
Celtrix Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Celtrix Pharma filed Critical Celtrix Pharma
Application granted granted Critical
Publication of ATE280234T1 publication Critical patent/ATE280234T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/545—IL-1
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/71—Receptors; Cell surface antigens; Cell surface determinants for growth factors; for growth regulators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/32—Fusion polypeptide fusions with soluble part of a cell surface receptor, "decoy receptors"
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/95—Fusion polypeptide containing a motif/fusion for degradation (ubiquitin fusions, PEST sequence)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT94925175T 1993-08-02 1994-08-02 Expression eines fusionspolypeptides, das ohne leadersequenz aus dem cytoplasma transportiert wird ATE280234T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/100,744 US5563046A (en) 1993-08-02 1993-08-02 Fusion polypeptides and proteins
PCT/US1994/008776 WO1995004076A1 (en) 1993-08-02 1994-08-02 Expression of fusion polypeptides transported out of the cytoplasm without leader sequences

Publications (1)

Publication Number Publication Date
ATE280234T1 true ATE280234T1 (de) 2004-11-15

Family

ID=22281315

Family Applications (1)

Application Number Title Priority Date Filing Date
AT94925175T ATE280234T1 (de) 1993-08-02 1994-08-02 Expression eines fusionspolypeptides, das ohne leadersequenz aus dem cytoplasma transportiert wird

Country Status (8)

Country Link
US (3) US5563046A (de)
EP (1) EP0722461B1 (de)
JP (1) JP3370094B2 (de)
AT (1) ATE280234T1 (de)
AU (1) AU688363B2 (de)
CA (1) CA2168429C (de)
DE (1) DE69434083T2 (de)
WO (1) WO1995004076A1 (de)

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Also Published As

Publication number Publication date
WO1995004076A1 (en) 1995-02-09
US5629172A (en) 1997-05-13
US5830706A (en) 1998-11-03
EP0722461A4 (de) 1997-02-26
EP0722461A1 (de) 1996-07-24
AU688363B2 (en) 1998-03-12
DE69434083T2 (de) 2006-02-09
EP0722461B1 (de) 2004-10-20
JP3370094B2 (ja) 2003-01-27
DE69434083D1 (de) 2004-11-25
CA2168429C (en) 2001-06-05
AU7519494A (en) 1995-02-28
JPH09504166A (ja) 1997-04-28
CA2168429A1 (en) 1995-02-09
US5563046A (en) 1996-10-08

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