ATE486943T1 - Schnelles screening-verfahren translationaler fusionspartner zur produktion rekombinanter proteine sowie durch screening damit erhaltene translationale fusionspartner - Google Patents

Schnelles screening-verfahren translationaler fusionspartner zur produktion rekombinanter proteine sowie durch screening damit erhaltene translationale fusionspartner

Info

Publication number
ATE486943T1
ATE486943T1 AT04808645T AT04808645T ATE486943T1 AT E486943 T1 ATE486943 T1 AT E486943T1 AT 04808645 T AT04808645 T AT 04808645T AT 04808645 T AT04808645 T AT 04808645T AT E486943 T1 ATE486943 T1 AT E486943T1
Authority
AT
Austria
Prior art keywords
fusion partners
translational fusion
screening
production
recombinant proteins
Prior art date
Application number
AT04808645T
Other languages
English (en)
Inventor
Jung-Hoon Sohn
Eui-Sung Choi
Jung-Hoon Bae
Eung-Suck Lee
Mi-Kyung Shin
Original Assignee
Korea Res Inst Of Bioscience
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR1020040003610A external-priority patent/KR100626753B1/ko
Priority claimed from KR1020040003957A external-priority patent/KR20050076139A/ko
Application filed by Korea Res Inst Of Bioscience filed Critical Korea Res Inst Of Bioscience
Application granted granted Critical
Publication of ATE486943T1 publication Critical patent/ATE486943T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/53—Colony-stimulating factor [CSF]
    • C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/55—IL-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT04808645T 2004-01-17 2004-12-30 Schnelles screening-verfahren translationaler fusionspartner zur produktion rekombinanter proteine sowie durch screening damit erhaltene translationale fusionspartner ATE486943T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR1020040003610A KR100626753B1 (ko) 2004-01-17 2004-01-17 재조합 단백질 생산용 맞춤형 단백질융합인자의 초고속선별 방법
KR1020040003957A KR20050076139A (ko) 2004-01-19 2004-01-19 단백질 분비 생산을 유도하는 단백질융합인자
PCT/KR2004/003517 WO2005068658A1 (en) 2004-01-17 2004-12-30 Rapid screening method of translational fusion partners for producing recombinant proteins and translational fusion partners screened therefrom

Publications (1)

Publication Number Publication Date
ATE486943T1 true ATE486943T1 (de) 2010-11-15

Family

ID=34797866

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04808645T ATE486943T1 (de) 2004-01-17 2004-12-30 Schnelles screening-verfahren translationaler fusionspartner zur produktion rekombinanter proteine sowie durch screening damit erhaltene translationale fusionspartner

Country Status (7)

Country Link
US (2) US8673822B2 (de)
EP (2) EP1713930B1 (de)
JP (2) JP5148879B2 (de)
AT (1) ATE486943T1 (de)
DE (1) DE602004029936D1 (de)
DK (2) DK2275556T3 (de)
WO (1) WO2005068658A1 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2275556T3 (da) 2004-01-17 2013-07-08 Korea Res Inst Of Bioscience Hurtig fremgangsmåde til screening af translationsfusionspartnere til fremstilling af rekombinante proteiner og translationsfusionpartnere screenet derfra
KR20070009269A (ko) * 2005-07-15 2007-01-18 한국생명공학연구원 재조합단백질 생산용 단백질융합인자 라이브러리 및이로부터 획득된 단백질융합인자
JP5524977B2 (ja) 2008-12-04 2014-06-18 コリア リサーチ インスティテュート オブ バイオサイエンス アンド バイオテクノロジー 高分泌性蛋白質のスクリーニングおよび組換え蛋白質の生産のための融合パートナーとしてのその用途
KR101291241B1 (ko) 2010-11-04 2013-08-01 한국생명공학연구원 효모에서 인체 상피세포 성장인자를 대량 생산하는 방법
CN114848614A (zh) * 2013-07-18 2022-08-05 曼金德公司 热稳定性干粉药物组合物和方法
CN104694523A (zh) * 2015-03-21 2015-06-10 江南大学 一种精氨酸脱亚胺酶的胞外表达方法及其应用
EP4453019A1 (de) * 2021-12-23 2024-10-30 Helsingin Yliopisto Verfahren zum screenen eines signalpeptids zur effizienten expression und sekretion eines heterologen polypeptids in säugerzellen
WO2025092927A1 (en) * 2023-10-31 2025-05-08 Chienyu Investment Co., Ltd. Oral formulation for activation of autologous stem cells

Family Cites Families (26)

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Publication number Priority date Publication date Assignee Title
WO1984004330A1 (en) * 1983-04-22 1984-11-08 Amgen Secretion of exogenous polypeptides from yeast
FI871919A7 (fi) * 1986-05-02 1987-11-03 Gist Brocades Nv Erityssignaaliselektiovektoreita solunulkoiseen proteiinisynteesiin Bacillus-bakteereilla
FR2648713B1 (fr) * 1989-06-26 1994-06-10 Commissariat Energie Atomique Proteines hybrides entre une enzyme extracytoplasmique et une autre proteine, leur procede de preparation, ainsi que leur application comme agent de diagnostic
US5212058A (en) * 1990-05-09 1993-05-18 Massachusetts Institute Of Technology Nucleic acid encoding ubiquitin-specific proteases
US5547871A (en) * 1993-01-25 1996-08-20 American Cyanamid Company Heterologous signal sequences for secretion of insect controlling proteins
US5536637A (en) * 1993-04-07 1996-07-16 Genetics Institute, Inc. Method of screening for cDNA encoding novel secreted mammalian proteins in yeast
US5563046A (en) * 1993-08-02 1996-10-08 Celtrix Pharmaceuticals, Inc. Fusion polypeptides and proteins
ATE197608T1 (de) * 1993-12-10 2000-12-15 Korea Inst Sci & Tech Signalsequenzen für die secretion heterologer proteine von hefe
EP0773296B1 (de) * 1995-02-03 2006-09-13 Asahi Glass Company Ltd. Gen für ein sekretionssignal und dieses enthaltender vektor
WO1997040146A1 (en) 1996-04-24 1997-10-30 Genetics Institute, Inc. Yeast invertase gene as reporter system for isolating cytokines
EP1170366A1 (de) 1996-04-24 2002-01-09 Genetics Institute, Inc. Secreted proteins obtained by using Yeast invertase gene as reporter system
US5952171A (en) * 1996-11-19 1999-09-14 Millennium Biotherapeutics, Inc. Method for identifying genes encoding secreted or membrane-associated proteins
US6136569A (en) * 1997-02-27 2000-10-24 Genentech, Inc. Method of creating a cDNA library enriched in signal sequences
US20060009633A9 (en) * 1997-11-13 2006-01-12 Genset, S.A. Complementary DNAs encoding proteins with signal peptides
US6548633B1 (en) * 1998-12-22 2003-04-15 Genset, S.A. Complementary DNA's encoding proteins with signal peptides
US6150098A (en) * 1998-02-20 2000-11-21 Amgen Inc. Methods for identifying novel secreted mammalian polypeptides
AU3193399A (en) * 1998-03-23 1999-10-18 Genentech Inc. Method of selection for genes encoding secreted and transmembrane proteins
US6753314B1 (en) * 1999-04-01 2004-06-22 Curagen Corporation Protein-protein complexes and methods of using same
CA2368098A1 (en) * 1999-06-25 2001-01-04 Genset S.A. Complementary dna's encoding proteins with signal peptides
US7029842B2 (en) * 2000-04-07 2006-04-18 Novozymes A/S Signal sequence trapping
AU4409101A (en) * 2000-04-07 2001-10-23 Novozymes As Signal sequence trapping
US20020127557A1 (en) * 2001-03-09 2002-09-12 Ruoying Tan Method for identification of cDNAs encoding signal peptides
US7314974B2 (en) * 2002-02-21 2008-01-01 Monsanto Technology, Llc Expression of microbial proteins in plants for production of plants with improved properties
DK2275556T3 (da) 2004-01-17 2013-07-08 Korea Res Inst Of Bioscience Hurtig fremgangsmåde til screening af translationsfusionspartnere til fremstilling af rekombinante proteiner og translationsfusionpartnere screenet derfra
KR20070009269A (ko) 2005-07-15 2007-01-18 한국생명공학연구원 재조합단백질 생산용 단백질융합인자 라이브러리 및이로부터 획득된 단백질융합인자
JP5524977B2 (ja) * 2008-12-04 2014-06-18 コリア リサーチ インスティテュート オブ バイオサイエンス アンド バイオテクノロジー 高分泌性蛋白質のスクリーニングおよび組換え蛋白質の生産のための融合パートナーとしてのその用途

Also Published As

Publication number Publication date
EP2275556A1 (de) 2011-01-19
DE602004029936D1 (de) 2010-12-16
DK1713930T3 (da) 2011-01-17
JP5662363B2 (ja) 2015-01-28
JP2007517519A (ja) 2007-07-05
WO2005068658A1 (en) 2005-07-28
US20070275385A1 (en) 2007-11-29
JP2012105675A (ja) 2012-06-07
US9012367B2 (en) 2015-04-21
JP5148879B2 (ja) 2013-02-20
EP1713930A4 (de) 2007-02-28
US8673822B2 (en) 2014-03-18
US20140220631A1 (en) 2014-08-07
DK2275556T3 (da) 2013-07-08
EP2275556B1 (de) 2013-04-24
EP1713930B1 (de) 2010-11-03
EP1713930A1 (de) 2006-10-25

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