ATE281532T1 - Methoden zur synthese von polynukleotiden durch ligation von multiplen oligomeren - Google Patents
Methoden zur synthese von polynukleotiden durch ligation von multiplen oligomerenInfo
- Publication number
- ATE281532T1 ATE281532T1 AT99937170T AT99937170T ATE281532T1 AT E281532 T1 ATE281532 T1 AT E281532T1 AT 99937170 T AT99937170 T AT 99937170T AT 99937170 T AT99937170 T AT 99937170T AT E281532 T1 ATE281532 T1 AT E281532T1
- Authority
- AT
- Austria
- Prior art keywords
- ligation
- synthesis
- oligomers
- methods
- polynucleotids
- Prior art date
Links
- 230000015572 biosynthetic process Effects 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 3
- 238000003786 synthesis reaction Methods 0.000 title abstract 3
- 102000003960 Ligases Human genes 0.000 abstract 1
- 108090000364 Ligases Proteins 0.000 abstract 1
- 229910019142 PO4 Inorganic materials 0.000 abstract 1
- 238000012300 Sequence Analysis Methods 0.000 abstract 1
- 230000003321 amplification Effects 0.000 abstract 1
- 238000004458 analytical method Methods 0.000 abstract 1
- 238000003556 assay Methods 0.000 abstract 1
- 238000010367 cloning Methods 0.000 abstract 1
- 230000000295 complement effect Effects 0.000 abstract 1
- 230000014509 gene expression Effects 0.000 abstract 1
- 238000002372 labelling Methods 0.000 abstract 1
- 238000012544 monitoring process Methods 0.000 abstract 1
- 230000035772 mutation Effects 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 102000040430 polynucleotide Human genes 0.000 abstract 1
- 108091033319 polynucleotide Proteins 0.000 abstract 1
- 239000002157 polynucleotide Substances 0.000 abstract 1
- 238000012216 screening Methods 0.000 abstract 1
- 230000002194 synthesizing effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Crystallography & Structural Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/121,887 US5998175A (en) | 1998-07-24 | 1998-07-24 | Methods of synthesizing and amplifying polynucleotides by ligation of multiple oligomers |
| US09/241,979 US6020138A (en) | 1998-07-24 | 1999-02-02 | Methods for detecting nucleic acids by ligation of multiple oligomers |
| US09/241,353 US6001614A (en) | 1998-07-24 | 1999-02-02 | Methods of synthesizing labeled polynucleotides by ligation of multiple oligomers |
| US09/245,984 US6013456A (en) | 1998-07-24 | 1999-02-05 | Methods of sequencing polynucleotides by ligation of multiple oligomers |
| PCT/US1999/014160 WO2000005412A1 (en) | 1998-07-24 | 1999-07-22 | Methods of synthesizing polynucleotides by ligation of multiple oligomers |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE281532T1 true ATE281532T1 (de) | 2004-11-15 |
Family
ID=27494390
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT99937170T ATE281532T1 (de) | 1998-07-24 | 1999-07-22 | Methoden zur synthese von polynukleotiden durch ligation von multiplen oligomeren |
Country Status (10)
| Country | Link |
|---|---|
| EP (1) | EP1017855B1 (de) |
| JP (1) | JP2002521036A (de) |
| AT (1) | ATE281532T1 (de) |
| AU (1) | AU772995B2 (de) |
| CA (1) | CA2304258C (de) |
| DE (1) | DE69921594T2 (de) |
| DK (1) | DK1017855T3 (de) |
| ES (1) | ES2233062T3 (de) |
| IL (1) | IL134729A (de) |
| WO (1) | WO2000005412A1 (de) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005529606A (ja) * | 2002-06-12 | 2005-10-06 | データスコープ・インベストメント・コーポレイション | ポリマー標識分子 |
| US7361821B2 (en) * | 2002-09-20 | 2008-04-22 | Intel Corporation | Controlled alignment of nanobarcodes encoding specific information for scanning probe microscopy (SPM) reading |
| WO2004038037A2 (en) * | 2002-09-20 | 2004-05-06 | Intel Corporation | Controlled alignment of nano-barcodes encoding specific information for scanning probe microscopy (spm) reading |
| WO2004044242A1 (en) * | 2002-11-14 | 2004-05-27 | John Nicholas Housby | Polymorphism assay |
| JP2007275006A (ja) * | 2006-04-10 | 2007-10-25 | Hitachi High-Technologies Corp | 核酸検出用プローブ作製法 |
| GB201612011D0 (en) | 2016-07-11 | 2016-08-24 | Glaxosmithkline Ip Dev Ltd | Novel processes for the production of oligonucleotides |
| CN110337497B (zh) * | 2017-04-13 | 2024-05-24 | 德国癌症研究公共权益基金会 | 通过多路连接标记寡核苷酸探针 |
| CN115852495B (zh) * | 2022-12-30 | 2023-09-15 | 苏州泓迅生物科技股份有限公司 | 一种基因突变文库的合成方法及其应用 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4883750A (en) * | 1984-12-13 | 1989-11-28 | Applied Biosystems, Inc. | Detection of specific sequences in nucleic acids |
| WO1989011211A2 (en) * | 1988-05-24 | 1989-11-30 | Gesellschaft Für Biotechnologische Forschung Mbh ( | Oligonucleotide bank and process for dna sequencing |
| ATE144556T1 (de) * | 1988-06-24 | 1996-11-15 | Amgen Inc | Verfahren und mittel zum nachweis von nukleinsäuresequenzen |
| US5132215A (en) * | 1988-09-15 | 1992-07-21 | Eastman Kodak Company | Method of making double-stranded dna sequences |
| WO1993020096A1 (en) * | 1991-05-08 | 1993-10-14 | Stratagene | Oligonucleotide libraries useful for producing primers |
| US5681943A (en) * | 1993-04-12 | 1997-10-28 | Northwestern University | Method for covalently linking adjacent oligonucleotides |
| DE4336911C2 (de) * | 1993-10-28 | 2002-11-21 | Biotechnolog Forschung Gmbh | Verfahren zur Primer-Herstellung und Template-Bank dafür |
| US5888731A (en) * | 1995-08-30 | 1999-03-30 | Visible Genetics Inc. | Method for identification of mutations using ligation of multiple oligonucleotide probes |
| US6312892B1 (en) * | 1996-07-19 | 2001-11-06 | Cornell Research Foundation, Inc. | High fidelity detection of nucleic acid differences by ligase detection reaction |
-
1999
- 1999-07-22 DE DE69921594T patent/DE69921594T2/de not_active Expired - Lifetime
- 1999-07-22 AU AU52052/99A patent/AU772995B2/en not_active Expired
- 1999-07-22 IL IL13472999A patent/IL134729A/xx not_active IP Right Cessation
- 1999-07-22 AT AT99937170T patent/ATE281532T1/de active
- 1999-07-22 DK DK99937170T patent/DK1017855T3/da active
- 1999-07-22 EP EP99937170A patent/EP1017855B1/de not_active Expired - Lifetime
- 1999-07-22 WO PCT/US1999/014160 patent/WO2000005412A1/en not_active Ceased
- 1999-07-22 ES ES99937170T patent/ES2233062T3/es not_active Expired - Lifetime
- 1999-07-22 JP JP2000561358A patent/JP2002521036A/ja active Pending
- 1999-07-22 CA CA2304258A patent/CA2304258C/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| AU772995B2 (en) | 2004-05-13 |
| JP2002521036A (ja) | 2002-07-16 |
| ES2233062T3 (es) | 2005-06-01 |
| WO2000005412A1 (en) | 2000-02-03 |
| DE69921594D1 (de) | 2004-12-09 |
| DK1017855T3 (da) | 2005-03-14 |
| IL134729A0 (en) | 2001-04-30 |
| CA2304258A1 (en) | 2000-02-03 |
| EP1017855B1 (de) | 2004-11-03 |
| AU5205299A (en) | 2000-02-14 |
| CA2304258C (en) | 2010-04-13 |
| IL134729A (en) | 2005-08-31 |
| DE69921594T2 (de) | 2005-11-10 |
| EP1017855A1 (de) | 2000-07-12 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification |
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