ATE283928T1 - Differenzierungsfärbung von bakterien unter verwendung von fluoreszenz - Google Patents

Differenzierungsfärbung von bakterien unter verwendung von fluoreszenz

Info

Publication number
ATE283928T1
ATE283928T1 AT99936969T AT99936969T ATE283928T1 AT E283928 T1 ATE283928 T1 AT E283928T1 AT 99936969 T AT99936969 T AT 99936969T AT 99936969 T AT99936969 T AT 99936969T AT E283928 T1 ATE283928 T1 AT E283928T1
Authority
AT
Austria
Prior art keywords
fluorescence
bacteria
red
differential staining
green
Prior art date
Application number
AT99936969T
Other languages
English (en)
Inventor
Paolo Fazii
Ettore Ciancaglini
Original Assignee
Paolo Fazii
Ettore Ciancaglini
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Paolo Fazii, Ettore Ciancaglini filed Critical Paolo Fazii
Application granted granted Critical
Publication of ATE283928T1 publication Critical patent/ATE283928T1/de

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/14Streptococcus; Staphylococcus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/06Quantitative determination
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/10Enterobacteria
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2304/00Chemical means of detecting microorganisms
    • C12Q2304/10DNA staining
    • C12Q2304/16Acridine orange

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT99936969T 1998-07-29 1999-07-27 Differenzierungsfärbung von bakterien unter verwendung von fluoreszenz ATE283928T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IT1998PE000017A IT1304854B1 (it) 1998-07-29 1998-07-29 Metodo di differenziazione cromatica dei microrganismi in fluorescenza
PCT/IT1999/000238 WO2000006765A1 (en) 1998-07-29 1999-07-27 Differential staining of bacteria using fluorescence

Publications (1)

Publication Number Publication Date
ATE283928T1 true ATE283928T1 (de) 2004-12-15

Family

ID=11393161

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99936969T ATE283928T1 (de) 1998-07-29 1999-07-27 Differenzierungsfärbung von bakterien unter verwendung von fluoreszenz

Country Status (6)

Country Link
EP (1) EP1029074B1 (de)
AT (1) ATE283928T1 (de)
AU (1) AU5193199A (de)
DE (1) DE69922334D1 (de)
IT (1) IT1304854B1 (de)
WO (1) WO2000006765A1 (de)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10024086C1 (de) * 2000-05-18 2001-09-27 Merck Patent Gmbh Phenolfreie Färbelösung
US9522396B2 (en) 2010-12-29 2016-12-20 S.D. Sight Diagnostics Ltd. Apparatus and method for automatic detection of pathogens
CN104169719B (zh) 2011-12-29 2017-03-08 思迪赛特诊断有限公司 用于检测生物样品中病原体的方法和系统
EP2999988A4 (de) 2013-05-23 2017-01-11 S.D. Sight Diagnostics Ltd. Verfahren und system zur bildgebung einer zellprobe
IL227276A0 (en) 2013-07-01 2014-03-06 Parasight Ltd A method and system for preparing a monolayer of cells, particularly suitable for diagnosis
US10831013B2 (en) 2013-08-26 2020-11-10 S.D. Sight Diagnostics Ltd. Digital microscopy systems, methods and computer program products
US10482595B2 (en) 2014-08-27 2019-11-19 S.D. Sight Diagnostics Ltd. System and method for calculating focus variation for a digital microscope
AU2016322966B2 (en) 2015-09-17 2021-10-14 S.D. Sight Diagnostics Ltd Methods and apparatus for detecting an entity in a bodily sample
US11733150B2 (en) 2016-03-30 2023-08-22 S.D. Sight Diagnostics Ltd. Distinguishing between blood sample components
US11307196B2 (en) 2016-05-11 2022-04-19 S.D. Sight Diagnostics Ltd. Sample carrier for optical measurements
EP3455626B1 (de) 2016-05-11 2025-08-06 S.D. Sight Diagnostics Ltd. Durchführung optischer messungen auf einer probe
AU2018369859B2 (en) 2017-11-14 2024-01-25 S.D. Sight Diagnostics Ltd Sample carrier for optical measurements
AU2020372024B2 (en) 2019-10-22 2026-03-12 S.D. Sight Diagnostics Ltd Accounting for errors in optical measurements
MX2022007127A (es) 2019-12-12 2022-07-11 S D Sight Diagnostics Ltd Generacion artificial de una imagen de frotis sanguineo en color.
AU2020400400B2 (en) 2019-12-12 2026-03-19 S.D. Sight Diagnostics Ltd Detecting platelets in a blood sample
BR112022011312A2 (pt) 2019-12-12 2022-08-23 S D Sight Diagnostics Ltd Análise de um analito disposto dentro de um meio

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4665024A (en) * 1984-10-01 1987-05-12 Becton, Dickinson And Company Fluorescent gram stain

Also Published As

Publication number Publication date
EP1029074A1 (de) 2000-08-23
EP1029074B1 (de) 2004-12-01
AU5193199A (en) 2000-02-21
ITPE980017A1 (it) 1998-10-29
IT1304854B1 (it) 2001-04-05
DE69922334D1 (de) 2005-01-05
WO2000006765A1 (en) 2000-02-10

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